| General information | |||
| TC008696 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 14200158..14202799 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008696 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04534 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
- >TC008696 RA
MARGKVKYR DVVGGRSKK SKKFDKKKT KPRKTYSLY RQDTKKPTK KRKFEASEE NYEKRQKVE KEAQPEQES SESEEEIDP MKELMSTFG SKLQHKQSL AISSSDESS DEEDENSPE DILEENEDT DEETLTKNE DTPDSEEDE DEVAVDSAQ NEDSDNLND PFVKHVCYE LHESVLKSL QSSPVLVND SVLNWPSLG RLLVQIPQF EELKEEKNS FTIEEKKKF APPGSVPKK MNTSDLKKP ENLFIKSQI IDNLSKAND SLKKDDTVF TDFQSELFS IINNYQDLY FTQRNFNNA EEIRFVYCL HAVNHILKT RLKVVHHNV RLSKKDDVP EDFRDQGLV RPKILMVVP FKDSAFKII QMIINILLP EDKGHVMNK KRFLEEYTG NEIPMPKKN PKPEDYELT FTGNTSDDF KIGITVTKK SLKLFADFY SSDIIIASP LGLRTIIGA EGEPQRDYD FLASIELLI LDQTELFSM QNWDHFIHV INHLHLQPK DSHGTDFSR VRTWSLNGW AQYYRQTLI FSSIVLPEI NSIFNKKCN NFAGKVKIS NPVELGSIG QVVVQIPHV FHKFEAPNA QAAIEARFE FFINKLLPL QRDSLMKQT MIYVPSYFD FVRLRNYFK KEELSFGQI CEYSKDGKV ARARDMFYH GDTHSLLYT ERFHFFNRV RVKGIRHLV FYQPPTFPH FYYEMCNLM QEANMNKKI GSLSNMTVN VIYSKYDVH RLAAIVGTE RATKMLQSE RNVHMMVTG AD
- >TC008696 RA
atggccaga ggtaaggta aagtaccga gacgtggtt ggaggccgg tcaaaaaaa tcgaaaaag tttgataaa aagaaaacc aaaccgaga aagacttac agcttgtat cgtcaagac actaaaaag cccactaag aagcgtaag tttgaggct agtgaagaa aattacgag aaacggcaa aaagttgaa aaagaagct cagcccgag caagagtct tcagaaagt gaggaggaa attgatccg atgaaggag ttaatgtct acttttggt tcaaaactg caacataaa caatcactt gcgatatca agtagcgac gaaagttca gacgaagaa gatgaaaac tctcctgag gacattctt gaggaaaat gaagacacg gacgaggaa acattaaca aaaaatgaa gacacccca gattcggaa gaagatgag gatgaggtg gcagttgat agcgcgcaa aatgaggat agtgataac ttgaatgat ccctttgta aaacatgtt tgttatgaa ctgcatgaa agtgtttta aagtcatta caatcctct ccagtattg gtcaacgat tctgtttta aactggcca agtttaggg cgcctgtta gttcaaata ccacaattt gaagaatta aaggaagag aaaaattcg tttacaata gaagaaaag aaaaaattt gcaccaccg ggttcagta cctaaaaaa atgaacacc tcagacctc aaaaaaccg gagaatttg ttcattaaa tcacaaatt atagataat ttatctaag gccaatgat tcactaaaa aaggatgat acagtattt actgatttt cagtctgaa ttgttttcg ataattaac aattatcaa gatttgtat tttacccaa agaaacttt aataatgct gaggaaatc agatttgtt tattgcctt catgccgta aatcacatt ttaaaaaca cggttaaaa gttgttcac cacaatgta cgtttatca aaaaaggac gacgttcct gaggatttt agagaccag ggattagtt aggcccaag attcttatg gttgttccc ttcaaagac tcggctttt aaaatcatc caaatgata ataaacata ctcctgcct gaagataaa ggtcacgta atgaacaaa aaaagattt ctggaggag tacacaggc aatgaaatt ccgatgccc aaaaagaac cccaaaccc gaagactac gaactgact ttcaccgga aacacaagc gacgatttt aagattggc attactgtt acaaaaaag agcctgaag ctgttcgct gacttttac tcctccgat attatcata gcttcgcct ttggggctc cggacaata atcggcgct gaaggcgaa ccccaacgt gactacgat tttctcgcc tccatagaa cttctaatt ctggaccaa accgaatta ttttcgatg caaaactgg gaccacttt attcacgtt ataaatcat ctccacttg cagccgaaa gactcacat gggactgat ttttcgcga gttcggacc tggagtttg aacggatgg gcgcaatat taccgacag actttgata ttttcgtcg attgttttg cccgagatt aactcaatt tttaataag aaatgtaac aatttcgct gggaaagtc aaaatctcg aatccagtc gagttgggg tcgattgga caagtcgtt gttcagata ccgcacgta tttcataaa tttgaggcc ccgaatgca caggccgca attgaggcc cggtttgag ttttttatc aataaactc ttacctctg cagagggac agtttgatg aaacagacg atgatttat gtgccgagt tatttcgat tttgtgcgt ttgaggaat tattttaag aaggaggag ctcagtttt gggcaaatt tgcgaatat tctaaggac ggtaaagta gcccgagcc cgagacatg ttttatcac ggagatact cactcttta ttgtatact gagcggttt cattttttt aatcgcgtc agagttaaa ggtatacgc catttagtt ttttatcaa ccgccgact tttcctcac ttttattat gaaatgtgt aatctaatg caggaagcc aacatgaat aaaaaaatc gggagttta tcgaatatg acggtaaat gtaatttat tcgaaatat gatgttcac cggttagcg gcaattgtt gggacagaa agggccaca aaaatgtta caatcggag agaaatgtc cacatgatg gtcaccggt gctgattaa
- >TC008696 RA
gttgacgta ttaacctca cttttatta taacctttc tcaaaaata aaaaacgtt aaaagaggc ctatctaca attttttat agaagacta gtgtattgc gctattttt tcacaaaaa cggtatggc cagaggtaa ggtaaagta ccgagacgt ggttggagg ccggtcaaa aaaatcgaa aaagtttga taaaaagaa aaccaaacc gagaaagac ttacagctt gtatcgtca agacactaa aaagcccac taagaagcg taagtttga ggctagtga agaaaatta cgagaaacg gcaaaaagt tgaaaaaga agctcagcc cgagcaaga gtcttcaga aagtgagga ggaaattga tccgatgaa ggagttaat gtctacttt tggttcaaa actgcaaca taaacaatc acttgcgat atcaagtag cgacgaaag ttcagacga agaagatga aaactctcc tgaggacat tcttgagga aaatgaaga cacggacga ggaaacatt aacaaaaaa tgaagacac cccagattc ggaagaaga tgaggatga ggtggcagt tgatagcgc gcaaaatga ggatagtga taacttgaa tgatccctt tgtaaaaca tgtttgtta tgaactgca tgaaagtgt tttaaagtc attacaatc ctctccagt attggtcaa cgattctgt tttaaactg gccaagttt agggcgcct gttagttca aataccaca atttgaaga attaaagga agagaaaaa ttcgtttac aatagaaga aaagaaaaa atttgcacc accgggttc agtacctaa aaaaatgaa cacctcaga cctcaaaaa accggagaa tttgttcat taaatcaca aattataga taatttatc taaggccaa tgattcact aaaaaagga tgatacagt atttactga ttttcagtc tgaattgtt ttcgataat taacaatta tcaagattt gtattttac ccaaagaaa ctttaataa tgctgagga aatcagatt tgtttattg ccttcatgc cgtaaatca cattttaaa aacacggtt aaaagttgt tcaccacaa tgtacgttt atcaaaaaa ggacgacgt tcctgagga ttttagaga ccagggatt agttaggcc caagattct tatggttgt tcccttcaa agactcggc ttttaaaat catccaaat gataataaa catactcct gcctgaaga taaaggtca cgtaatgaa caaaaaaag atttctgga ggagtacac aggcaatga aattccgat gcccaaaaa gaaccccaa acccgaaga ctacgaact gactttcac cggaaacac aagcgacga ttttaagat tggcattac tgttacaaa aaagagcct gaagctgtt cgctgactt ttactcctc cgatattat catagcttc gcctttggg gctccggac aataatcgg cgctgaagg cgaacccca acgtgacta cgattttct cgcctccat agaacttct aattctgga ccaaaccga attattttc gatgcaaaa ctgggacca ctttattca cgttataaa tcatctcca cttgcagcc gaaagactc acatgggac tgatttttc gcgagttcg gacctggag tttgaacgg atgggcgca atattaccg acagacttt gatattttc gtcgattgt tttgcccga gattaactc aatttttaa taagaaatg taacaattt cgctgggaa agtcaaaat ctcgaatcc agtcgagtt ggggtcgat tggacaagt cgttgttca gataccgca cgtatttca taaatttga ggccccgaa tgcacaggc cgcaattga ggcccggtt tgagttttt tatcaataa actcttacc tctgcagag ggacagttt gatgaaaca gacgatgat ttatgtgcc gagttattt cgattttgt gcgtttgag gaattattt taagaagga ggagctcag ttttgggca aatttgcga atattctaa ggacggtaa agtagcccg agcccgaga catgtttta tcacggaga tactcactc tttattgta tactgagcg gtttcattt ttttaatcg cgtcagagt taaaggtat acgccattt agtttttta tcaaccgcc gacttttcc tcactttta ttatgaaat gtgtaatct aatgcagga agccaacat gaataaaaa aatcgggag tttatcgaa tatgacggt aaatgtaat ttattcgaa atatgatgt tcaccggtt agcggcaat tgttgggac agaaagggc cacaaaaat gttacaatc ggagagaaa tgtccacat gatggtcac cggtgctga ttaaaagac agaaattaa agatctaaa tgtgattgt attttttat gttaataaa aaattaaca aaatc
