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Gene information
General information
TC008696 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14200158..14202799
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008696
    iBeetle screen iB_04534

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008696 RA
      MARGKVKYR DVVGGRSKK SKKFDKKKT KPRKTYSLY 
RQDTKKPTK KRKFEASEE NYEKRQKVE KEAQPEQES 
SESEEEIDP MKELMSTFG SKLQHKQSL AISSSDESS 
DEEDENSPE DILEENEDT DEETLTKNE DTPDSEEDE 
DEVAVDSAQ NEDSDNLND PFVKHVCYE LHESVLKSL 
QSSPVLVND SVLNWPSLG RLLVQIPQF EELKEEKNS 
FTIEEKKKF APPGSVPKK MNTSDLKKP ENLFIKSQI 
IDNLSKAND SLKKDDTVF TDFQSELFS IINNYQDLY 
FTQRNFNNA EEIRFVYCL HAVNHILKT RLKVVHHNV 
RLSKKDDVP EDFRDQGLV RPKILMVVP FKDSAFKII 
QMIINILLP EDKGHVMNK KRFLEEYTG NEIPMPKKN 
PKPEDYELT FTGNTSDDF KIGITVTKK SLKLFADFY 
SSDIIIASP LGLRTIIGA EGEPQRDYD FLASIELLI 
LDQTELFSM QNWDHFIHV INHLHLQPK DSHGTDFSR 
VRTWSLNGW AQYYRQTLI FSSIVLPEI NSIFNKKCN 
NFAGKVKIS NPVELGSIG QVVVQIPHV FHKFEAPNA 
QAAIEARFE FFINKLLPL QRDSLMKQT MIYVPSYFD 
FVRLRNYFK KEELSFGQI CEYSKDGKV ARARDMFYH 
GDTHSLLYT ERFHFFNRV RVKGIRHLV FYQPPTFPH 
FYYEMCNLM QEANMNKKI GSLSNMTVN VIYSKYDVH 
RLAAIVGTE RATKMLQSE RNVHMMVTG AD
    CDS sequence
    • >TC008696 RA
      atggccaga ggtaaggta aagtaccga gacgtggtt 
ggaggccgg tcaaaaaaa tcgaaaaag tttgataaa 
aagaaaacc aaaccgaga aagacttac agcttgtat 
cgtcaagac actaaaaag cccactaag aagcgtaag 
tttgaggct agtgaagaa aattacgag aaacggcaa 
aaagttgaa aaagaagct cagcccgag caagagtct 
tcagaaagt gaggaggaa attgatccg atgaaggag 
ttaatgtct acttttggt tcaaaactg caacataaa 
caatcactt gcgatatca agtagcgac gaaagttca 
gacgaagaa gatgaaaac tctcctgag gacattctt 
gaggaaaat gaagacacg gacgaggaa acattaaca 
aaaaatgaa gacacccca gattcggaa gaagatgag 
gatgaggtg gcagttgat agcgcgcaa aatgaggat 
agtgataac ttgaatgat ccctttgta aaacatgtt 
tgttatgaa ctgcatgaa agtgtttta aagtcatta 
caatcctct ccagtattg gtcaacgat tctgtttta 
aactggcca agtttaggg cgcctgtta gttcaaata 
ccacaattt gaagaatta aaggaagag aaaaattcg 
tttacaata gaagaaaag aaaaaattt gcaccaccg 
ggttcagta cctaaaaaa atgaacacc tcagacctc 
aaaaaaccg gagaatttg ttcattaaa tcacaaatt 
atagataat ttatctaag gccaatgat tcactaaaa 
aaggatgat acagtattt actgatttt cagtctgaa 
ttgttttcg ataattaac aattatcaa gatttgtat 
tttacccaa agaaacttt aataatgct gaggaaatc 
agatttgtt tattgcctt catgccgta aatcacatt 
ttaaaaaca cggttaaaa gttgttcac cacaatgta 
cgtttatca aaaaaggac gacgttcct gaggatttt 
agagaccag ggattagtt aggcccaag attcttatg 
gttgttccc ttcaaagac tcggctttt aaaatcatc 
caaatgata ataaacata ctcctgcct gaagataaa 
ggtcacgta atgaacaaa aaaagattt ctggaggag 
tacacaggc aatgaaatt ccgatgccc aaaaagaac 
cccaaaccc gaagactac gaactgact ttcaccgga 
aacacaagc gacgatttt aagattggc attactgtt 
acaaaaaag agcctgaag ctgttcgct gacttttac 
tcctccgat attatcata gcttcgcct ttggggctc 
cggacaata atcggcgct gaaggcgaa ccccaacgt 
gactacgat tttctcgcc tccatagaa cttctaatt 
ctggaccaa accgaatta ttttcgatg caaaactgg 
gaccacttt attcacgtt ataaatcat ctccacttg 
cagccgaaa gactcacat gggactgat ttttcgcga 
gttcggacc tggagtttg aacggatgg gcgcaatat 
taccgacag actttgata ttttcgtcg attgttttg 
cccgagatt aactcaatt tttaataag aaatgtaac 
aatttcgct gggaaagtc aaaatctcg aatccagtc 
gagttgggg tcgattgga caagtcgtt gttcagata 
ccgcacgta tttcataaa tttgaggcc ccgaatgca 
caggccgca attgaggcc cggtttgag ttttttatc 
aataaactc ttacctctg cagagggac agtttgatg 
aaacagacg atgatttat gtgccgagt tatttcgat 
tttgtgcgt ttgaggaat tattttaag aaggaggag 
ctcagtttt gggcaaatt tgcgaatat tctaaggac 
ggtaaagta gcccgagcc cgagacatg ttttatcac 
ggagatact cactcttta ttgtatact gagcggttt 
cattttttt aatcgcgtc agagttaaa ggtatacgc 
catttagtt ttttatcaa ccgccgact tttcctcac 
ttttattat gaaatgtgt aatctaatg caggaagcc 
aacatgaat aaaaaaatc gggagttta tcgaatatg 
acggtaaat gtaatttat tcgaaatat gatgttcac 
cggttagcg gcaattgtt gggacagaa agggccaca 
aaaatgtta caatcggag agaaatgtc cacatgatg 
gtcaccggt gctgattaa
    mRNA sequence
    • >TC008696 RA
      gttgacgta ttaacctca cttttatta taacctttc 
tcaaaaata aaaaacgtt aaaagaggc ctatctaca 
attttttat agaagacta gtgtattgc gctattttt 
tcacaaaaa cggtatggc cagaggtaa ggtaaagta 
ccgagacgt ggttggagg ccggtcaaa aaaatcgaa 
aaagtttga taaaaagaa aaccaaacc gagaaagac 
ttacagctt gtatcgtca agacactaa aaagcccac 
taagaagcg taagtttga ggctagtga agaaaatta 
cgagaaacg gcaaaaagt tgaaaaaga agctcagcc 
cgagcaaga gtcttcaga aagtgagga ggaaattga 
tccgatgaa ggagttaat gtctacttt tggttcaaa 
actgcaaca taaacaatc acttgcgat atcaagtag 
cgacgaaag ttcagacga agaagatga aaactctcc 
tgaggacat tcttgagga aaatgaaga cacggacga 
ggaaacatt aacaaaaaa tgaagacac cccagattc 
ggaagaaga tgaggatga ggtggcagt tgatagcgc 
gcaaaatga ggatagtga taacttgaa tgatccctt 
tgtaaaaca tgtttgtta tgaactgca tgaaagtgt 
tttaaagtc attacaatc ctctccagt attggtcaa 
cgattctgt tttaaactg gccaagttt agggcgcct 
gttagttca aataccaca atttgaaga attaaagga 
agagaaaaa ttcgtttac aatagaaga aaagaaaaa 
atttgcacc accgggttc agtacctaa aaaaatgaa 
cacctcaga cctcaaaaa accggagaa tttgttcat 
taaatcaca aattataga taatttatc taaggccaa 
tgattcact aaaaaagga tgatacagt atttactga 
ttttcagtc tgaattgtt ttcgataat taacaatta 
tcaagattt gtattttac ccaaagaaa ctttaataa 
tgctgagga aatcagatt tgtttattg ccttcatgc 
cgtaaatca cattttaaa aacacggtt aaaagttgt 
tcaccacaa tgtacgttt atcaaaaaa ggacgacgt 
tcctgagga ttttagaga ccagggatt agttaggcc 
caagattct tatggttgt tcccttcaa agactcggc 
ttttaaaat catccaaat gataataaa catactcct 
gcctgaaga taaaggtca cgtaatgaa caaaaaaag 
atttctgga ggagtacac aggcaatga aattccgat 
gcccaaaaa gaaccccaa acccgaaga ctacgaact 
gactttcac cggaaacac aagcgacga ttttaagat 
tggcattac tgttacaaa aaagagcct gaagctgtt 
cgctgactt ttactcctc cgatattat catagcttc 
gcctttggg gctccggac aataatcgg cgctgaagg 
cgaacccca acgtgacta cgattttct cgcctccat 
agaacttct aattctgga ccaaaccga attattttc 
gatgcaaaa ctgggacca ctttattca cgttataaa 
tcatctcca cttgcagcc gaaagactc acatgggac 
tgatttttc gcgagttcg gacctggag tttgaacgg 
atgggcgca atattaccg acagacttt gatattttc 
gtcgattgt tttgcccga gattaactc aatttttaa 
taagaaatg taacaattt cgctgggaa agtcaaaat 
ctcgaatcc agtcgagtt ggggtcgat tggacaagt 
cgttgttca gataccgca cgtatttca taaatttga 
ggccccgaa tgcacaggc cgcaattga ggcccggtt 
tgagttttt tatcaataa actcttacc tctgcagag 
ggacagttt gatgaaaca gacgatgat ttatgtgcc 
gagttattt cgattttgt gcgtttgag gaattattt 
taagaagga ggagctcag ttttgggca aatttgcga 
atattctaa ggacggtaa agtagcccg agcccgaga 
catgtttta tcacggaga tactcactc tttattgta 
tactgagcg gtttcattt ttttaatcg cgtcagagt 
taaaggtat acgccattt agtttttta tcaaccgcc 
gacttttcc tcactttta ttatgaaat gtgtaatct 
aatgcagga agccaacat gaataaaaa aatcgggag 
tttatcgaa tatgacggt aaatgtaat ttattcgaa 
atatgatgt tcaccggtt agcggcaat tgttgggac 
agaaagggc cacaaaaat gttacaatc ggagagaaa 
tgtccacat gatggtcac cggtgctga ttaaaagac 
agaaattaa agatctaaa tgtgattgt attttttat 
gttaataaa aaattaaca aaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY