Status
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Gene information
General information
TC008696 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14200158..14202799
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008696
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04534

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008696 RA
      MARGKVKYR DVVGGRSKK SKKFDKKKT KPRKTYSLY 
      RQDTKKPTK KRKFEASEE NYEKRQKVE KEAQPEQES 
      SESEEEIDP MKELMSTFG SKLQHKQSL AISSSDESS 
      DEEDENSPE DILEENEDT DEETLTKNE DTPDSEEDE 
      DEVAVDSAQ NEDSDNLND PFVKHVCYE LHESVLKSL 
      QSSPVLVND SVLNWPSLG RLLVQIPQF EELKEEKNS 
      FTIEEKKKF APPGSVPKK MNTSDLKKP ENLFIKSQI 
      IDNLSKAND SLKKDDTVF TDFQSELFS IINNYQDLY 
      FTQRNFNNA EEIRFVYCL HAVNHILKT RLKVVHHNV 
      RLSKKDDVP EDFRDQGLV RPKILMVVP FKDSAFKII 
      QMIINILLP EDKGHVMNK KRFLEEYTG NEIPMPKKN 
      PKPEDYELT FTGNTSDDF KIGITVTKK SLKLFADFY 
      SSDIIIASP LGLRTIIGA EGEPQRDYD FLASIELLI 
      LDQTELFSM QNWDHFIHV INHLHLQPK DSHGTDFSR 
      VRTWSLNGW AQYYRQTLI FSSIVLPEI NSIFNKKCN 
      NFAGKVKIS NPVELGSIG QVVVQIPHV FHKFEAPNA 
      QAAIEARFE FFINKLLPL QRDSLMKQT MIYVPSYFD 
      FVRLRNYFK KEELSFGQI CEYSKDGKV ARARDMFYH 
      GDTHSLLYT ERFHFFNRV RVKGIRHLV FYQPPTFPH 
      FYYEMCNLM QEANMNKKI GSLSNMTVN VIYSKYDVH 
      RLAAIVGTE RATKMLQSE RNVHMMVTG AD
    CDS sequence
    • >TC008696 RA
      atggccaga ggtaaggta aagtaccga gacgtggtt 
      ggaggccgg tcaaaaaaa tcgaaaaag tttgataaa 
      aagaaaacc aaaccgaga aagacttac agcttgtat 
      cgtcaagac actaaaaag cccactaag aagcgtaag 
      tttgaggct agtgaagaa aattacgag aaacggcaa 
      aaagttgaa aaagaagct cagcccgag caagagtct 
      tcagaaagt gaggaggaa attgatccg atgaaggag 
      ttaatgtct acttttggt tcaaaactg caacataaa 
      caatcactt gcgatatca agtagcgac gaaagttca 
      gacgaagaa gatgaaaac tctcctgag gacattctt 
      gaggaaaat gaagacacg gacgaggaa acattaaca 
      aaaaatgaa gacacccca gattcggaa gaagatgag 
      gatgaggtg gcagttgat agcgcgcaa aatgaggat 
      agtgataac ttgaatgat ccctttgta aaacatgtt 
      tgttatgaa ctgcatgaa agtgtttta aagtcatta 
      caatcctct ccagtattg gtcaacgat tctgtttta 
      aactggcca agtttaggg cgcctgtta gttcaaata 
      ccacaattt gaagaatta aaggaagag aaaaattcg 
      tttacaata gaagaaaag aaaaaattt gcaccaccg 
      ggttcagta cctaaaaaa atgaacacc tcagacctc 
      aaaaaaccg gagaatttg ttcattaaa tcacaaatt 
      atagataat ttatctaag gccaatgat tcactaaaa 
      aaggatgat acagtattt actgatttt cagtctgaa 
      ttgttttcg ataattaac aattatcaa gatttgtat 
      tttacccaa agaaacttt aataatgct gaggaaatc 
      agatttgtt tattgcctt catgccgta aatcacatt 
      ttaaaaaca cggttaaaa gttgttcac cacaatgta 
      cgtttatca aaaaaggac gacgttcct gaggatttt 
      agagaccag ggattagtt aggcccaag attcttatg 
      gttgttccc ttcaaagac tcggctttt aaaatcatc 
      caaatgata ataaacata ctcctgcct gaagataaa 
      ggtcacgta atgaacaaa aaaagattt ctggaggag 
      tacacaggc aatgaaatt ccgatgccc aaaaagaac 
      cccaaaccc gaagactac gaactgact ttcaccgga 
      aacacaagc gacgatttt aagattggc attactgtt 
      acaaaaaag agcctgaag ctgttcgct gacttttac 
      tcctccgat attatcata gcttcgcct ttggggctc 
      cggacaata atcggcgct gaaggcgaa ccccaacgt 
      gactacgat tttctcgcc tccatagaa cttctaatt 
      ctggaccaa accgaatta ttttcgatg caaaactgg 
      gaccacttt attcacgtt ataaatcat ctccacttg 
      cagccgaaa gactcacat gggactgat ttttcgcga 
      gttcggacc tggagtttg aacggatgg gcgcaatat 
      taccgacag actttgata ttttcgtcg attgttttg 
      cccgagatt aactcaatt tttaataag aaatgtaac 
      aatttcgct gggaaagtc aaaatctcg aatccagtc 
      gagttgggg tcgattgga caagtcgtt gttcagata 
      ccgcacgta tttcataaa tttgaggcc ccgaatgca 
      caggccgca attgaggcc cggtttgag ttttttatc 
      aataaactc ttacctctg cagagggac agtttgatg 
      aaacagacg atgatttat gtgccgagt tatttcgat 
      tttgtgcgt ttgaggaat tattttaag aaggaggag 
      ctcagtttt gggcaaatt tgcgaatat tctaaggac 
      ggtaaagta gcccgagcc cgagacatg ttttatcac 
      ggagatact cactcttta ttgtatact gagcggttt 
      cattttttt aatcgcgtc agagttaaa ggtatacgc 
      catttagtt ttttatcaa ccgccgact tttcctcac 
      ttttattat gaaatgtgt aatctaatg caggaagcc 
      aacatgaat aaaaaaatc gggagttta tcgaatatg 
      acggtaaat gtaatttat tcgaaatat gatgttcac 
      cggttagcg gcaattgtt gggacagaa agggccaca 
      aaaatgtta caatcggag agaaatgtc cacatgatg 
      gtcaccggt gctgattaa
    mRNA sequence
    • >TC008696 RA
      gttgacgta ttaacctca cttttatta taacctttc 
      tcaaaaata aaaaacgtt aaaagaggc ctatctaca 
      attttttat agaagacta gtgtattgc gctattttt 
      tcacaaaaa cggtatggc cagaggtaa ggtaaagta 
      ccgagacgt ggttggagg ccggtcaaa aaaatcgaa 
      aaagtttga taaaaagaa aaccaaacc gagaaagac 
      ttacagctt gtatcgtca agacactaa aaagcccac 
      taagaagcg taagtttga ggctagtga agaaaatta 
      cgagaaacg gcaaaaagt tgaaaaaga agctcagcc 
      cgagcaaga gtcttcaga aagtgagga ggaaattga 
      tccgatgaa ggagttaat gtctacttt tggttcaaa 
      actgcaaca taaacaatc acttgcgat atcaagtag 
      cgacgaaag ttcagacga agaagatga aaactctcc 
      tgaggacat tcttgagga aaatgaaga cacggacga 
      ggaaacatt aacaaaaaa tgaagacac cccagattc 
      ggaagaaga tgaggatga ggtggcagt tgatagcgc 
      gcaaaatga ggatagtga taacttgaa tgatccctt 
      tgtaaaaca tgtttgtta tgaactgca tgaaagtgt 
      tttaaagtc attacaatc ctctccagt attggtcaa 
      cgattctgt tttaaactg gccaagttt agggcgcct 
      gttagttca aataccaca atttgaaga attaaagga 
      agagaaaaa ttcgtttac aatagaaga aaagaaaaa 
      atttgcacc accgggttc agtacctaa aaaaatgaa 
      cacctcaga cctcaaaaa accggagaa tttgttcat 
      taaatcaca aattataga taatttatc taaggccaa 
      tgattcact aaaaaagga tgatacagt atttactga 
      ttttcagtc tgaattgtt ttcgataat taacaatta 
      tcaagattt gtattttac ccaaagaaa ctttaataa 
      tgctgagga aatcagatt tgtttattg ccttcatgc 
      cgtaaatca cattttaaa aacacggtt aaaagttgt 
      tcaccacaa tgtacgttt atcaaaaaa ggacgacgt 
      tcctgagga ttttagaga ccagggatt agttaggcc 
      caagattct tatggttgt tcccttcaa agactcggc 
      ttttaaaat catccaaat gataataaa catactcct 
      gcctgaaga taaaggtca cgtaatgaa caaaaaaag 
      atttctgga ggagtacac aggcaatga aattccgat 
      gcccaaaaa gaaccccaa acccgaaga ctacgaact 
      gactttcac cggaaacac aagcgacga ttttaagat 
      tggcattac tgttacaaa aaagagcct gaagctgtt 
      cgctgactt ttactcctc cgatattat catagcttc 
      gcctttggg gctccggac aataatcgg cgctgaagg 
      cgaacccca acgtgacta cgattttct cgcctccat 
      agaacttct aattctgga ccaaaccga attattttc 
      gatgcaaaa ctgggacca ctttattca cgttataaa 
      tcatctcca cttgcagcc gaaagactc acatgggac 
      tgatttttc gcgagttcg gacctggag tttgaacgg 
      atgggcgca atattaccg acagacttt gatattttc 
      gtcgattgt tttgcccga gattaactc aatttttaa 
      taagaaatg taacaattt cgctgggaa agtcaaaat 
      ctcgaatcc agtcgagtt ggggtcgat tggacaagt 
      cgttgttca gataccgca cgtatttca taaatttga 
      ggccccgaa tgcacaggc cgcaattga ggcccggtt 
      tgagttttt tatcaataa actcttacc tctgcagag 
      ggacagttt gatgaaaca gacgatgat ttatgtgcc 
      gagttattt cgattttgt gcgtttgag gaattattt 
      taagaagga ggagctcag ttttgggca aatttgcga 
      atattctaa ggacggtaa agtagcccg agcccgaga 
      catgtttta tcacggaga tactcactc tttattgta 
      tactgagcg gtttcattt ttttaatcg cgtcagagt 
      taaaggtat acgccattt agtttttta tcaaccgcc 
      gacttttcc tcactttta ttatgaaat gtgtaatct 
      aatgcagga agccaacat gaataaaaa aatcgggag 
      tttatcgaa tatgacggt aaatgtaat ttattcgaa 
      atatgatgt tcaccggtt agcggcaat tgttgggac 
      agaaagggc cacaaaaat gttacaatc ggagagaaa 
      tgtccacat gatggtcac cggtgctga ttaaaagac 
      agaaattaa agatctaaa tgtgattgt attttttat 
      gttaataaa aaattaaca aaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY