General information | |||
TC008664 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 13787628..13790225 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008664 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04532 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
- >TC008664 RA
MGTAVSKNF ELSTNDYLL KFVSTQAIS QNDPFWNRL VAFNISPPM TSNDQLALE SKIENLCQL LLKNNHTSG NLGSIIDVF LSRATELLT ATNTDASLF IWQLFNILF IIRCIVKFL CETVTEEQL IEQIEVNGN GTRLETFVD ALVKVIVDV EVTDATYGI HLEAITTLL VFLSVQLHS GRKSDQSNI YRLIMRGKH TTHAPLFIK CLLTNYMKQ AKVPVGFGA NTGHSLVLG IAAELWSIL TFSRKSDEV LVSETSDYQ EVPLATQSL LLVLVLVHH WTTKNNPYR NSLFLCMNS ADNNPIAPI QANMFSRID YNVLYATIC KKASGDVTT LLLYLLIHR NDSFRAFLL SRTDLDHLI IPILKTLYN APNSNSHHI YMSLIILLI LSEDDSFNK SIHEIKLKN IVWYTERTL TEVSLGGLL VLVVIRTVQ YNMLKMRDK YLHTNCLAA LANMSAQFR DLHPYVSQR LVSLFETLA KRYQKLSTK LWNGKSEKN SEYAITVSQ DGDDMEQDI TVLEEVLRM VLEIFNSCL SSQLVNNPN LVYTLLYNK HIFEPFKEN VAFQDILQN IDIIIKYFS SLLDDKTQQ NEVDAYQVL NVIQQGAKR WPKDKLKKF PDLKFKYVE EDQPEEFFI PYVWTLVSQ QSILHWTND NSPLLATVC
- >TC008664 RA
atgggtacg gctgttagc aaaaatttt gaattgtcc acgaatgat tatttactt aaatttgta agcactcaa gcgatatca caaaatgac cccttttgg aaccgactg gtggctttc aacatttct ccacctatg acaagtaat gaccagcta gcgttagag tcaaaaatt gaaaacctt tgccaactg ttgctgaaa aataaccac acctcagga aatttgggt tcaattatt gacgtgttc ttgtcccga gctaccgag cttttgact gccaccaac accgacgca agcttattc atttggcag ttatttaac attctgttc attattcgc tgcattgtt aagtttctt tgcgaaacc gtgactgag gagcagcta atagagcaa attgaagta aacggcaat gggacaaga cttgagact ttcgtagac gctctagtt aaagttatc gtcgatgtt gaagttaca gatgccacg tatggcata cacctcgag gccattacc acacttcta gtattttta tcagtgcaa ttacattca gggcgaaag tcagaccaa agcaatatt tatagactt ataatgagg ggcaagcac acaacgcac gccccttta ttcatcaaa tgcttgcta actaattac atgaaacag gcgaaggtg ccggtgggg tttggggcc aacactggc cacagcctc gttctgggg atcgcagcg gaattgtgg tctatttta acgttcagc agaaaatcc gatgaagtt ttggtgtct gaaactagc gattatcag gaggtacct ttagccacc cagagtttg ctgctggtt ttagtgtta gtgcaccat tggaccact aagaacaac ccttacagg aatagccta tttttgtgt atgaatagt gccgacaat aatccaata gccccgatt caagccaat atgttttcg aggatagat tacaacgtt ctttacgca acaatttgc aaaaaagcg tcaggggac gtgaccaca ttattactc tatttacta atccaccgg aatgattca tttagggcc tttcttttg agtcgaacg gatttagat cacttgatt attccaatt ttgaagact ttgtataac gccccgaac agcaactcg catcatatc tacatgtca cttattatc ctactaata ttgagtgag gatgattct ttcaacaaa tcaatccat gaaattaaa ctgaaaaat atcgtgtgg tatacggag cggaccctc actgaggtc tcgcttggc ggcttgctg gtccttgtg gtgatacga acagtacaa tacaatatg ctgaaaatg cgtgataaa tacctgcac acaaattgt ttggcggct ttggcgaat atgtcggca cagtttcga gatttgcat ccgtacgtt agccaacgt ttggtttca ctgtttgaa actttggcg aaacggtat cagaaatta tccacaaag ttatggaac ggcaaaagt gagaagaac tcggagtac gccatcaca gtgtctcaa gacggtgac gatatggag caggatatc acagttctt gaggaggtt ttgaggatg gttttggaa attttcaat tcgtgtctt tccagtcag ttggtcaat aacccaaat ctagtttac acgcttttg tacaataaa catattttc gaaccgttt aaggaaaat gttgcgttt caagacata ctccaaaat attgacatt ataattaaa tacttttca agtttgttg gacgataaa acgcaacaa aacgaagtg gatgcctat caagttctt aacgttatt caacaaggt gcaaagcgg tggcctaaa gacaagttg aagaaattt cccgatttg aagttcaaa tatgtggag gaagatcaa ccagaggag tttttcata ccctatgtt tggacttta gttagccag caatctatc ctccactgg actaacgat aattcgcca ctactagcg actgtttgc taa
- >TC008664 RA
gttcgttca aatgtcatt tgacaggtg acgtgttta ctttttacg ataaggtta tgtcctagc aaatttatg gaaaaaatg ttaatggtg tgcgttata cattaaaat tgagcgtaa ataaaaatg ggtacggct gttagcaaa aattttgaa ttgtccacg aatgattat ttacttaaa tttgtaagc actcaagcg atatcacaa aatgacccc ttttggaac cgactggtg gctttcaac atttctcca cctatgaca agtaatgac cagctagcg ttagagtca aaaattgaa aacctttgc caactgttg ctgaaaaat aaccacacc tcaggaaat ttgggttca attattgac gtgttcttg tcccgagct accgagctt ttgactgcc accaacacc gacgcaagc ttattcatt tggcagtta tttaacatt ctgttcatt attcgctgc attgttaag tttctttgc gaaaccgtg actgaggag cagctaata gagcaaatt gaagtaaac ggcaatggg acaagactt gagactttc gtagacgct ctagttaaa gttatcgtc gatgttgaa gttacagat gccacgtat ggcatacac ctcgaggcc attaccaca cttctagta tttttatca gtgcaatta cattcaggg cgaaagtca gaccaaagc aatatttat agacttata atgaggggc aagcacaca acgcacgcc cctttattc atcaaatgc ttgctaact aattacatg aaacaggcg aaggtgccg gtggggttt ggggccaac actggccac agcctcgtt ctggggatc gcagcggaa ttgtggtct attttaacg ttcagcaga aaatccgat gaagttttg gtgtctgaa actagcgat tatcaggag gtaccttta gccacccag agtttgctg ctggtttta gtgttagtg caccattgg accactaag aacaaccct tacaggaat agcctattt ttgtgtatg aatagtgcc gacaataat ccaatagcc ccgattcaa gccaatatg ttttcgagg atagattac aacgttctt tacgcaaca atttgcaaa aaagcgtca ggggacgtg accacatta ttactctat ttactaatc caccggaat gattcattt agggccttt cttttgagt cgaacggat ttagatcac ttgattatt ccaattttg aagactttg tataacgcc ccgaacagc aactcgcat catatctac atgtcactt attatccta ctaatattg agtgaggat gattctttc aacaaatca atccatgaa attaaactg aaaaatatc gtgtggtat acggagcgg accctcact gaggtctcg cttggcggc ttgctggtc cttgtggtg atacgaaca gtacaatac aatatgctg aaaatgcgt gataaatac ctgcacaca aattgtttg gcggctttg gcgaatatg tcggcacag tttcgagat ttgcatccg tacgttagc caacgtttg gtttcactg tttgaaact ttggcgaaa cggtatcag aaattatcc acaaagtta tggaacggc aaaagtgag aagaactcg gagtacgcc atcacagtg tctcaagac ggtgacgat atggagcag gatatcaca gttcttgag gaggttttg aggatggtt ttggaaatt ttcaattcg tgtctttcc agtcagttg gtcaataac ccaaatcta gtttacacg cttttgtac aataaacat attttcgaa ccgtttaag gaaaatgtt gcgtttcaa gacatactc caaaatatt gacattata attaaatac ttttcaagt ttgttggac gataaaacg caacaaaac gaagtggat gcctatcaa gttcttaac gttattcaa caaggtgca aagcggtgg cctaaagac aagttgaag aaatttccc gatttgaag ttcaaatat gtggaggaa gatcaacca gaggagttt ttcataccc tatgtttgg actttagtt agccagcaa tctatcctc cactggact aacgataat tcgccacta ctagcgact gtttgctaa cattttaat gtgtaatta cttatttat tatattatt gtttaaact gagttattg tttcataat ttatcattt gttccttga atgttagat attcatttt attttccag tatgtattt tgtaaaaga aacttattg tacaataaa cttaaagta acttg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
