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Gene information
General information
TC008652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13677523..13678879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008652
    iBeetle screen iB_04528

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008652 RA
      MVTFHKLLF TILGLLIAT IFLLSQYRT ESTPAQTLI 
      KRSVDGKPT FLGNNVTKT ILYWTPMFQ SPHFYLGTG 
      SKIFEKCAY KNCYATYVK NELPVEKFH AIMFHAVEY 
      QEKLFGKPQ KRNPNQFYI FSNKESPVN TPSFIKDFN 
      NFYNWTMTY RLDSDILRP YGFLIKKKT DYELPTVEE 
      IQKRPKKIA WFVSNCKTS SQRELLVNE IQKEIHVDV 
      YGKCSALHC EKDNTEACY DKMERDYKF YLSFENSIC 
      EDYVTEKLY NVLQRNIVP IVYGGADYN TLAPPKSVI 
      NVMDFMSVK DLVKHIKYL DSHPEKYLK FLEWKKDYI 
      VETSSTRSL CTLCQKLHE PIKQKIYSD ITKWWAGKN 
      KNKCMVNKN GFLDKYLLQ S
    CDS sequence
    • >TC008652 RA
      atggtaacg tttcataaa ctattattt accattctt 
      gggttatta atagcaacg atcttcctc ttatcacaa 
      taccgaacc gaatcgaca cccgcgcaa acattaata 
      aaacgcagt gtagacggc aaaccaacc tttctgggc 
      aataatgtc accaaaacg attctttac tggacccca 
      atgttccaa agtccccat ttctacctc ggcactggg 
      tcaaaaatt ttcgaaaaa tgtgcctac aagaactgt 
      tacgccacc tacgttaaa aatgaactt cccgtcgaa 
      aaattccac gctataatg ttccacgcc gtggaatac 
      caggagaaa ttgttcggc aagccccaa aaacgcaac 
      ccgaatcaa ttttatatt ttctctaac aaggaatca 
      ccagtgaat actccgtcc ttcatcaag gattttaac 
      aacttttat aattggaca atgacgtac cgattggat 
      tccgatatc cttcgtcct tacggcttc cttattaag 
      aaaaaaacc gactacgag ttaccaaca gttgaagaa 
      attcaaaaa cggccgaaa aaaattgcg tggtttgtc 
      tcgaattgt aaaacctcg agtcaaaga gaactactg 
      gtcaatgaa atccaaaaa gagatacac gtagatgtt 
      tacggaaaa tgtagtgct ttgcactgt gagaaagat 
      aatacggaa gcctgctac gataagatg gaaagggac 
      tacaaattt tacctctcg tttgaaaat tcgatttgt 
      gaagactac gttacagag aagttgtac aatgttctg 
      caacgaaat atagtgcca attgtgtac ggtggtgcc 
      gattataac actctcgcg ccgcctaaa tctgtgatt 
      aatgtgatg gattttatg tcggttaaa gacttagtt 
      aagcatata aaatatctg gacagtcac cccgaaaag 
      tatttaaag tttctcgag tggaaaaag gactacatt 
      gtcgaaact tcaagtacc cggtcgctt tgtacgcta 
      tgtcagaaa ttacacgag cccataaaa caaaaaatt 
      tacagtgat attacgaaa tggtgggcg ggaaaaaat 
      aagaataaa tgtatggtt aataaaaat ggttttctg 
      gataagtat ttactccag tcgtga
    mRNA sequence
    • >TC008652 RA
      tgtcttatc aataaataa aaatggtaa cgtttcata 
      aactattat ttaccattc ttgggttat taatagcaa 
      cgatcttcc tcttatcac aataccgaa ccgaatcga 
      cacccgcgc aaacattaa taaaacgca gtgtagacg 
      gcaaaccaa cctttctgg gcaataatg tcaccaaaa 
      cgattcttt actggaccc caatgttcc aaagtcccc 
      atttctacc tcggcactg ggtcaaaaa ttttcgaaa 
      aatgtgcct acaagaact gttacgcca cctacgtta 
      aaaatgaac ttcccgtcg aaaaattcc acgctataa 
      tgttccacg ccgtggaat accaggaga aattgttcg 
      gcaagcccc aaaaacgca acccgaatc aattttata 
      ttttctcta acaaggaat caccagtga atactccgt 
      ccttcatca aggatttta acaactttt ataattgga 
      caatgacgt accgattgg attccgata tccttcgtc 
      cttacggct tccttatta agaaaaaaa ccgactacg 
      agttaccaa cagttgaag aaattcaaa aacggccga 
      aaaaaattg cgtggtttg tctcgaatt gtaaaacct 
      cgagtcaaa gagaactac tggtcaatg aaatccaaa 
      aagagatac acgtagatg tttacggaa aatgtagtg 
      ctttgcact gtgagaaag ataatacgg aagcctgct 
      acgataaga tggaaaggg actacaaat tttacctct 
      cgtttgaaa attcgattt gtgaagact acgttacag 
      agaagttgt acaatgttc tgcaacgaa atatagtgc 
      caattgtgt acggtggtg ccgattata acactctcg 
      cgccgccta aatctgtga ttaatgtga tggatttta 
      tgtcggtta aagacttag ttaagcata taaaatatc 
      tggacagtc accccgaaa agtatttaa agtttctcg 
      agtggaaaa aggactaca ttgtcgaaa cttcaagta 
      cccggtcgc tttgtacgc tatgtcaga aattacacg 
      agcccataa aacaaaaaa tttacagtg atattacga 
      aatggtggg cgggaaaaa ataagaata aatgtatgg 
      ttaataaaa atggttttc tggataagt atttactcc 
      agtcgtgaa tcgattcgt gagccagtg gttacgaaa 
      attttattt acttaaagc ctttacttg acgaatttg 
      tgccaaaga tacttgtaa agtatattt aatgacaaa 
      gtccttgaa ttcttcgta gttgttagc aattgtatg 
      ttaatattg tactctttt acttagatt tgtatgtac 
      gaattaata aattgtttt aaatttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY