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Gene information
General information
TC008636 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13482853..13485682
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008636
    iBeetle screen iB_04527

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008636 RA
      MSDVSLAKT PVEELAGGD QPEDSEQPE VYKPYKVFI 
      NHVDSYHSK YISAYITDQ IYGLSKLMR QGGPGEEAP 
      GEEAAAVKP AMQDLPKDK YEFIGTLTS VYKEPSEDI 
      LYTLSEDDE DFREKILKC GFIIYDITK YPKQIEKAL 
      KTLSILTEQ LNDISKIGP KTYEQFNET KIFILISTI 
      MTWGLTKPL DPDDPDLPF SEVDYRKRR PHPNYKEHY 
      ACEKEVIKI GQQYKDKLK TYVVCTGLT YGEEEDILD 
      FFFKTAWYN EVLPIFDNG NNKIPFIHV RDLAKALHG 
      LMENAPPKP QYILAVEQT PTTLKKFIK TLSKALGDG 
      KVANIMSEE AFLYKNMTQ TVFERCTVN LNMEPVFLV 
      ENLQLEWQS ELTIAENML QVVREFTTK RNLRPVKII 
      IHGPPGSGK THLAKKMCE YYNSKYVSI PTMLEDYIK 
      IWKESLIRP EKPKLEETE YLGEEELEE EVEDEEDPQ 
      QILEQIREM ELFMEAGIE KIPDDYITG LLRSYLAKN 
      FCQNRGYVL DDYPRLPAQ AKELFGQGE NIGGEEEEM 
      EAPDFAEKV LPNLVVGLV ATDEFITQR IMKMQEEEI 
      RDTPYAEEV ILERLKEFR ENNTDENTV LNYFDELEI 
      DILLVEVKD DDNEEAFSL IANQMGPPS TFGLTPEEE 
      EELRRIQEQ ERQLALEKE ELNKQLELE KAEDERKNK 
      MEAWSEMLE KLQMEEEKI LVAQSEPLR HYLMKYVFP 
      ILTKGLIEL ATLKPDDPV DFLAEYLFK ENPEGKMFD 
      PSYNVECED LYTEFRGYI DKIIDENEA FD
    CDS sequence
    • >TC008636 RA
      atgtcagat gtctcgctt gcaaaaact ccggttgag 
      gaactggcc ggaggagac caacccgaa gactccgaa 
      caacctgaa gtgtacaaa ccttacaaa gtttttatc 
      aaccatgtg gattcttat cactccaaa tacatctcg 
      gcctacata acggaccaa atttatgga ttgtccaag 
      ctgatgagg caagggggc ccaggcgag gaggccccg 
      ggagaggaa gcagcggcg gtaaagccc gccatgcaa 
      gacctcccc aaagacaaa tacgaattc atcggcaca 
      cttacctcg gtttataaa gaaccgtcc gaagacatt 
      ctttacacc ctcagcgaa gatgatgaa gattttcgc 
      gaaaagatc ctaaagtgt ggcttcata atttacgac 
      attacgaaa tatccgaaa caaattgaa aaggcgctc 
      aaaacgttg tctattctt acagaacag ttaaatgat 
      attagtaaa atcgggccg aaaacctac gagcagttt 
      aacgaaacc aagattttt attctcatt tcgaccatc 
      atgacctgg ggattgaca aagccatta gaccccgat 
      gacccggac ttgcctttt tcggaagtg gactatagg 
      aaacggagg ccacatcct aattataag gaacattac 
      gcttgcgaa aaagaagtt ataaaaatt gggcaacaa 
      tataaagac aaattaaaa acgtatgtt gtatgcacc 
      ggcctgacg tatggcgaa gaagaagat attttggac 
      ttttttttc aaaacggca tggtacaat gaagtcttg 
      ccaattttt gataacggc aacaacaaa attcctttt 
      atccacgtc cgcgattta gcaaaagcc cttcatggg 
      ctaatggaa aacgcgcct ccgaagcct cagtatatt 
      ttagcagtc gagcaaacg ccgacaact ctcaaaaag 
      tttatcaaa actttaagc aaggcttta ggtgacgga 
      aaagtggcg aatatcatg tccgaggaa gctttcctc 
      tacaaaaac atgacccaa actgttttt gagcgatgc 
      actgttaat cttaacatg gaaccggtg tttctcgtt 
      gaaaatctg caattagag tggcagagc gaattaact 
      attgctgaa aacatgctt caagtggtc cgcgaattt 
      acaacaaaa cgaaaccta aggcctgtg aaaataata 
      atacatggg cctcccggc tccggcaaa actcacttg 
      gcgaaaaaa atgtgcgaa tattataac tccaaatat 
      gtctcaatt cccacaatg ttggaagat tacataaaa 
      atttggaaa gaaagtctc ataaggcct gaaaaaccc 
      aaattggaa gaaaccgag taccttggc gaagaggaa 
      ctggaagaa gaggtggaa gacgaagaa gacccgcaa 
      caaattttg gagcaaata cgagaaatg gaactgttt 
      atggaagct gggattgag aaaatccct gatgattac 
      atcaccggg ttattacga tcgtatttg gcgaaaaac 
      ttttgccaa aatcggggt tacgtcctc gatgattac 
      cctcgcttg ccggctcaa gccaaagaa cttttcggg 
      caaggtgaa aacataggg ggcgaagaa gaagaaatg 
      gaagctcca gactttgcc gaaaaagtt ttgcccaac 
      cttgttgtc ggcttggtg gccactgat gaatttatt 
      acccaaagg atcatgaaa atgcaagag gaagaaata 
      agggacact ccttacgct gaagaagta attttggaa 
      agactgaag gagtttcgc gagaacaac actgatgaa 
      aacactgtt ttgaattat tttgatgag ctggaaatt 
      gatatatta cttgtggaa gttaaagac gatgataat 
      gaggaagcc ttttctctc atagctaat caaatgggt 
      ccaccgtcc actttcggt ttaacacca gaggaagaa 
      gaagagttg aggagaata caagaacag gagagacaa 
      ttagctttg gagaaagaa gagctgaat aaacaactc 
      gaattggaa aaagcggaa gacgagcgc aagaataaa 
      atggaagct tggtcagaa atgcttgaa aagttgcaa 
      atggaagaa gagaagatt ttagtggcc cagagcgaa 
      cctttgaga cactatttg atgaaatac gtatttccg 
      attctaact aaaggtttg atagaacta gctacgttg 
      aaaccggat gatccagtt gattttctt gctgaatat 
      cttttcaaa gaaaatccc gagggtaaa atgtttgat 
      ccttcgtac aatgtcgag tgcgaggat ctttacacc 
      gagtttcgg ggatacatt gacaaaatt attgacgag 
      aacgaggca tttgattga
    mRNA sequence
    • >TC008636 RA
      ttaattttg atttttttg gaaacgttg tcaaacatt 
      tttaaacta aaatgtcag atgtctcgc ttgcaaaaa 
      ctccggttg aggaactgg ccggaggag accaacccg 
      aagactccg aacaacctg aagtgtaca aaccttaca 
      aagttttta tcaaccatg tggattctt atcactcca 
      aatacatct cggcctaca taacggacc aaatttatg 
      gattgtcca agctgatga ggcaagggg gcccaggcg 
      aggaggccc cgggagagg aagcagcgg cggtaaagc 
      ccgccatgc aagacctcc ccaaagaca aatacgaat 
      tcatcggca cacttacct cggtttata aagaaccgt 
      ccgaagaca ttctttaca ccctcagcg aagatgatg 
      aagattttc gcgaaaaga tcctaaagt gtggcttca 
      taatttacg acattacga aatatccga aacaaattg 
      aaaaggcgc tcaaaacgt tgtctattc ttacagaac 
      agttaaatg atattagta aaatcgggc cgaaaacct 
      acgagcagt ttaacgaaa ccaagattt ttattctca 
      tttcgacca tcatgacct ggggattga caaagccat 
      tagaccccg atgacccgg acttgcctt tttcggaag 
      tggactata ggaaacgga ggccacatc ctaattata 
      aggaacatt acgcttgcg aaaaagaag ttataaaaa 
      ttgggcaac aatataaag acaaattaa aaacgtatg 
      ttgtatgca ccggcctga cgtatggcg aagaagaag 
      atattttgg acttttttt tcaaaacgg catggtaca 
      atgaagtct tgccaattt ttgataacg gcaacaaca 
      aaattcctt ttatccacg tccgcgatt tagcaaaag 
      cccttcatg ggctaatgg aaaacgcgc ctccgaagc 
      ctcagtata ttttagcag tcgagcaaa cgccgacaa 
      ctctcaaaa agtttatca aaactttaa gcaaggctt 
      taggtgacg gaaaagtgg cgaatatca tgtccgagg 
      aagctttcc tctacaaaa acatgaccc aaactgttt 
      ttgagcgat gcactgtta atcttaaca tggaaccgg 
      tgtttctcg ttgaaaatc tgcaattag agtggcaga 
      gcgaattaa ctattgctg aaaacatgc ttcaagtgg 
      tccgcgaat ttacaacaa aacgaaacc taaggcctg 
      tgaaaataa taatacatg ggcctcccg gctccggca 
      aaactcact tggcgaaaa aaatgtgcg aatattata 
      actccaaat atgtctcaa ttcccacaa tgttggaag 
      attacataa aaatttgga aagaaagtc tcataaggc 
      ctgaaaaac ccaaattgg aagaaaccg agtaccttg 
      gcgaagagg aactggaag aagaggtgg aagacgaag 
      aagacccgc aacaaattt tggagcaaa tacgagaaa 
      tggaactgt ttatggaag ctgggattg agaaaatcc 
      ctgatgatt acatcaccg ggttattac gatcgtatt 
      tggcgaaaa acttttgcc aaaatcggg gttacgtcc 
      tcgatgatt accctcgct tgccggctc aagccaaag 
      aacttttcg ggcaaggtg aaaacatag ggggcgaag 
      aagaagaaa tggaagctc cagactttg ccgaaaaag 
      ttttgccca accttgttg tcggcttgg tggccactg 
      atgaattta ttacccaaa ggatcatga aaatgcaag 
      aggaagaaa taagggaca ctccttacg ctgaagaag 
      taattttgg aaagactga aggagtttc gcgagaaca 
      acactgatg aaaacactg ttttgaatt attttgatg 
      agctggaaa ttgatatat tacttgtgg aagttaaag 
      acgatgata atgaggaag ccttttctc tcatagcta 
      atcaaatgg gtccaccgt ccactttcg gtttaacac 
      cagaggaag aagaagagt tgaggagaa tacaagaac 
      aggagagac aattagctt tggagaaag aagagctga 
      ataaacaac tcgaattgg aaaaagcgg aagacgagc 
      gcaagaata aaatggaag cttggtcag aaatgcttg 
      aaaagttgc aaatggaag aagagaaga ttttagtgg 
      cccagagcg aacctttga gacactatt tgatgaaat 
      acgtatttc cgattctaa ctaaaggtt tgatagaac 
      tagctacgt tgaaaccgg atgatccag ttgattttc 
      ttgctgaat atcttttca aagaaaatc ccgagggta 
      aaatgtttg atccttcgt acaatgtcg agtgcgagg 
      atctttaca ccgagtttc ggggataca ttgacaaaa 
      ttattgacg agaacgagg catttgatt gaaagacga 
      tttttgaat aatacattt tattttgta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY