Status
Please wait ...
Gene information
General information
TC008629 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13374943..13457891
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008629
    iBeetle screen iB_04526

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Comment: every thoratic segment was separatly blistered on the outer side. 2 blisters per segment in all dead prepupa

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    thorax partially blistered - Number of animals showing the phenotype: 5
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008629 RA
      MDVLQKNYD DGIHPGYMD GGAGAGLYE PHVAHRPGL 
      QGLHHSPHL NHAMHPYHA NHVNPTANH VMGGAVPDV 
      GKRDKDAIY GHPLFPLLA LIFEKCELA TCTPREPGV 
      AGGDVCSSE SFNEDIAVF SKQIRQEKP YYIADPEVD 
      SLMVQAIQV LRFHLLELE KVHELCDNF CHRYISCLK 
      GKMPIDLVI DERDGGKPP ELTGSTNGD GGARSNADS 
      TSHTDGAST PDVRPPSSS LSYGGPVND DVRSPGTPG 
      PLSQAPASQ QSLDASDPD AMGKWCPRR EWSSPPDAR 
      AASDAARRG VLYSSVFLG SPGDASNAS IGSGEGTGE 
      EDDDTNGKK NQKKRGIFP KVATNILRA WLFQHLTHP 
      YPSEDQKKQ LAQDTGLTI LQVNNWFIN ARRRIVQPM 
      IDQSNRAVF SPHAGPSGA YTDDASAAW RSSLPQPVR 
      ALSRRVLRT PPVRSPLAC DNDNGHINM RLLNVIILC 
      TKCKCSAPV FS
    CDS sequence
    • >TC008629 RA
      atggatgtg cttcaaaaa aattatgac gacggcatc 
      caccctggg tacatggac ggcggcgcc ggggcgggt 
      ttgtacgaa ccccacgtg gcgcacagg ccggggttg 
      caaggactt caccattca ccccacctg aaccatgcc 
      atgcatccg taccatgcg aaccatgtc aacccaacc 
      gccaaccac gtcatgggt ggagcggtg ccggacgtc 
      ggcaaaagg gacaaggac gccatctac ggtcacccg 
      ctgtttccc ctgctggct ctcattttc gaaaagtgc 
      gagttggca acgtgtacc cctcgagag cctggagtg 
      gcgggagga gacgtctgc tcctcggag tcgttcaac 
      gaagacata gccgttttc tccaaacag atccgacaa 
      gaaaagccc tattacata gcagatccc gaggtagat 
      tcattaatg gtccaggct atacaagta cttcggttt 
      cacctttta gaattggaa aaggtccat gagttgtgt 
      gataatttc tgccatcgg tatatcagt tgtctgaaa 
      ggcaaaatg cccatcgac ttggtgata gacgagagg 
      gacggaggc aagccgccc gaattgacg ggatcgacg 
      aatggagat ggtggtgct aggagtaac gcggattcg 
      acatcacac acggacggt gcgagtacg ccggatgtc 
      aggccgccg tcgtcgtcg ttatcgtac ggaggcccg 
      gtgaatgac gacgtgagg tcgcccgga acacctgga 
      cctctatct caagcaccc gcatcccag caaagtctc 
      gacgcctcg gatccagac gccatgggc aaatggtgt 
      ccgaggcga gaatggtcg tcgcctcca gacgctcga 
      gcggccagc gacgctgcg cgaagaggc gtcctttac 
      tcctccgtc ttcctcgga agtcctggt gatgccagt 
      aatgccagc atcgggagc ggggaggga acaggcgag 
      gaagatgac gacacgaac gggaagaag aatcaaaaa 
      aagagaggc atcttccct aaggtcgcc accaacata 
      ctcagagcg tggctgttc caacaccta acgcatcct 
      tatccctcg gaagaccaa aaaaagcag ttggcgcaa 
      gatacgggt ttaacgata ctacaagtc aacaactgg 
      ttcattaat gccaggcgg agaatagtt caacctatg 
      atcgaccag tcgaataga gcagtgttt tctccgcac 
      gcagggcct agcggagcg tacacagat gatgcatcg 
      gccgcctgg agatccagc cttccacaa ccagtacgc 
      gcactatcc cgcagagta ctacggaca ccacctgtg 
      agatccccc ctcgcttgt gacaacgac aatggacat 
      ataaatatg cgactctta aatgtgatt attctctgt 
      acaaagtgt aaatgtagt gccccagtg tttagttaa
    mRNA sequence
    • >TC008629 RA
      tcattcatc tgagcccca ctctgcccc cttcgttcg 
      gcgtgattg acagttggc agacgactg cggaccgcg 
      caggatctc cgcacgcgc ttcggacac ggcagctcg 
      gcgcaccat caccaccga gagatctga gaccccatc 
      caccgaccc catgcccga gggggcccc cagcacctg 
      ctcgcaccg gcccagtgt acacgacag tgcagtgaa 
      ccgcagtga gtgtgataa aagtgcgcg ccaagagtg 
      actctgacg gtgctacgg tgcgaccac ccggtgcag 
      ttcatggct cagccacgg gtgtaaaaa attcttttt 
      gaccgattt ttaaagaat agtgcgaaa cgattcgac 
      atggatgtg cttcaaaaa aattatgac gacggcatc 
      caccctggg tacatggac ggcggcgcc ggggcgggt 
      ttgtacgaa ccccacgtg gcgcacagg ccggggttg 
      caaggactt caccattca ccccacctg aaccatgcc 
      atgcatccg taccatgcg aaccatgtc aacccaacc 
      gccaaccac gtcatgggt ggagcggtg ccggacgtc 
      ggcaaaagg gacaaggac gccatctac ggtcacccg 
      ctgtttccc ctgctggct ctcattttc gaaaagtgc 
      gagttggca acgtgtacc cctcgagag cctggagtg 
      gcgggagga gacgtctgc tcctcggag tcgttcaac 
      gaagacata gccgttttc tccaaacag atccgacaa 
      gaaaagccc tattacata gcagatccc gaggtagat 
      tcattaatg gtccaggct atacaagta cttcggttt 
      cacctttta gaattggaa aaggtccat gagttgtgt 
      gataatttc tgccatcgg tatatcagt tgtctgaaa 
      ggcaaaatg cccatcgac ttggtgata gacgagagg 
      gacggaggc aagccgccc gaattgacg ggatcgacg 
      aatggagat ggtggtgct aggagtaac gcggattcg 
      acatcacac acggacggt gcgagtacg ccggatgtc 
      aggccgccg tcgtcgtcg ttatcgtac ggaggcccg 
      gtgaatgac gacgtgagg tcgcccgga acacctgga 
      cctctatct caagcaccc gcatcccag caaagtctc 
      gacgcctcg gatccagac gccatgggc aaatggtgt 
      ccgaggcga gaatggtcg tcgcctcca gacgctcga 
      gcggccagc gacgctgcg cgaagaggc gtcctttac 
      tcctccgtc ttcctcgga agtcctggt gatgccagt 
      aatgccagc atcgggagc ggggaggga acaggcgag 
      gaagatgac gacacgaac gggaagaag aatcaaaaa 
      aagagaggc atcttccct aaggtcgcc accaacata 
      ctcagagcg tggctgttc caacaccta acgcatcct 
      tatccctcg gaagaccaa aaaaagcag ttggcgcaa 
      gatacgggt ttaacgata ctacaagtc aacaactgg 
      ttcattaat gccaggcgg agaatagtt caacctatg 
      atcgaccag tcgaataga gcagtgttt tctccgcac 
      gcagggcct agcggagcg tacacagat gatgcatcg 
      gccgcctgg agatccagc cttccacaa ccagtacgc 
      gcactatcc cgcagagta ctacggaca ccacctgtg 
      agatccccc ctcgcttgt gacaacgac aatggacat 
      ataaatatg cgactctta aatgtgatt attctctgt 
      acaaagtgt aaatgtagt gccccagtg tttagttaa 
      cgggaagga cttgtacaa aagtattag actactcgt 
      gtgctgact gctgtacat agatagtgg taggtgcgg 
      gccgacccc ggagcggtg cccggaccg cggtgtata 
      ctgaaggga cagccgcaa ccgtgtaaa gtgatgtgg 
      tctcgtagc ttaggttcc tcgatgtgt taccttaca 
      ataccctcg tcctagtcc ccatccagt ccagaaacg 
      atcagcccg atcctcctc ctcttcttc actctgtcc 
      gccgtcacg tttcttaaa aaaaaatct tcaaaaagt 
      acgacgcgt acaaaaaaa taatccaag gttgtgctg 
      ttgtaaatt actagttgt tatatctgt tgtgatttt 
      tttgctatt tgaggaaaa aacccgaac tctccttgt 
      cccgatgat gatatacga tacgatata cgtaactac 
      tgaacctag agaacaaat cgtcgtgga ggtggccca 
      gcggacccc ttcgcccta cgactagaa taataatat 
      caatacaca agtatacag aatgtcgca aagtcactc 
      aggtggtcc atggctgat acctaaatt attatcgta 
      ccactgtta tagttaatt caagacacc acgcatcca 
      gatcgcagt tttcgttca tttgtacca ctctgatga 
      ttttgttta gttacttgc cagggacaa acggaggac 
      tggccggct gattccgga atgtccgca caagccttg 
      cctggttat tgttattgt tgttttgta aaaaagaaa 
      cggcatttc agaagaacc cgtcagtcg tcccgtatt 
      aaagtttag cgacaaggc ggaatttca tttcgatga 
      ttctaaatt aaaccgtca ggccttgtc ctggactag 
      ttttgtgtc cgtgcaagt ccttcgagg ccgccgccc 
      ccaatacac gacgtatac gactttagt gatgttata 
      ggttattat tatatgaaa ttatggcgt acacaatgg 
      cggcgacga ggcggagtt gcacggctc acactcacc 
      tattgtttg aaacttgtg tgtattctt cgttacctt 
      tgttagggt cagggcggg gggctacaa gtatactga 
      tttttacac tccgattta tagtttttg atctgatgt 
      actctcgag tgcgttgta catatttaa tgcccgcgc 
      cgacacgat acgattccc tctgcccca ggcccagtc 
      gttttttgt acatacaat tacctccga tgagaatgt 
      tggctgttc gttttaagt gatttccat catgaatca 
      ttattcgcc ttatccaat gtatacgaa aaaactaaa 
      atcaataca gatcaatga gagaatatc gtacctctg 
      tgttatgta tgagaggaa actttattg tgttctgct 
      ttttaatga aagtatata ttaatatta taaacgatt 
      atgggacga gatacctct aaactaatt tggtaacgc 
      cgtgtcctc tgtatagtg tttttttga aacgtgagc 
      attaaatgc tacatcttg tgtacttat ataaaatgt 
      gtttttaat actaccgaa ccactatcc ctccctgtt 
      cagttttaa taataatag tacaaattc tagttttac 
      tgtagtgct atatgtgac tgtcctctc cttccgccc 
      tccgtcaca cgatccgcc gatgtacag cagtgcgta 
      gcacaaaaa gggaaacaa tccctaaaa tccacggcc 
      gcaaagccg ccagatccg gcacctatt gttttcctt 
      ttttgttat gcacattca tttcaaaat ttctctttc 
      tttcatctt aattttagt ttcctccaa tttgtcacg 
      atcgcatta ttttcgtat tatgctgca agatgggac 
      gtttaactt ttctgtact gtgattgaa ttattgtta 
      attaagtgc aatttatgt tcatgtaaa taaacaatt 
      atttcaaat ttcaatact aataaacaa acaaaagta 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY