Status
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Gene information
General information
TC032973
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008590,TC008589
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1626958..1640545
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032973
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva segment random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032973 RA
      MEDHNKDET NFNLLQDKR LKPNGHEIK AYKRRWIIL 
      TIFIVYAAV NSYQWIEYS IINNIITRY YNVTPVMVD 
      WTSIIYMAL YAPLVIPAS YILDKYGLR AAGLIGGLG 
      TALGTSIKV FSIGRESFW IVLLGQAVV SASQLLILC 
      LPPRIAAVW FKPNEVSTA CSLGVFGTQ LGAALGFVL 
      SPMLVKNRE NVEEIGHDL KILCWGLTA VILPTAIAI 
      IFYFPMQPP KPPSITQVE ERNRTEKFS AKLFFQSIS 
      VLMKNMPFV IHVIAYGIN IGVFSAIGT LLNQLVLRY 
      FKHLPDAEE FAGRAGLVM IVIGMIGSI LFGIFLDKT 
      HKYKETTVY TYLLSSVSI LAFMFALEM KSKILVYIA 
      IGVVGLFTN AYMPVGFEF AMELTFPSS EGTTTGLLM 
      APSQVLGVV FALMLGQLN VVIGPFCVS AMEHDIVIE 
      KFLPTENRN QEIVLYKKR WLILTLYIL YAAINAFQW 
      FEYSIIANV LMKFYNVSA VSVDWTSII YMALYMPMV 
      IPVSYLMEK LDLRQIAAI GVLGTALGT IVKVYSLSP 
      DQMWVTYLG QTILSSMQV FILPLPPRI ASVWFGSKE 
      VSLACALGV FGTQLGQAI GFVVPPIVV RYSEDVETI 
      GRDLGVLVK GLAWLTLVV TVAVLTYFS AHPTTPPND 
      INNERTHFF KSLKNLFRN RSFNFHTVA YGINIAVFT 
      SISTLLNQF FLFHFPKSE EDAGRVGLI MVILGMMGA 
      IAFGYILDK THRYKEITL CMYIASFLS VIAFMFSLE 
      SGSKILVYI SGALVGLFI NAYMPVGLE LAIELTYPE 
      PESTSSGIL ISMTQTLGV LFTLMLGES YVKVEAWLD 
      LIH
    CDS sequence
    • >TC032973 RA
      atggaagat cataataag gatgaaaca aatttcaat 
      ctactccaa gataagcgg ttgaaacca aatggtcac 
      gaaatcaag gcttacaaa cgacgatgg ataatttta 
      acaattttc atagtttac gctgctgta aactcgtac 
      caatggatc gaatattca attatcaac aacataatt 
      acaagatat tacaacgtt actcctgtg atggtggac 
      tggacatca atcatttac atggccctc tatgcacct 
      ttagtcata ccagcttcc tacattttg gataaatat 
      ggtttacgt gccgctgga ctaattgga ggactagga 
      acagcatta ggaacctca attaaagta ttttcaatc 
      ggacgtgaa tccttctgg attgtcctt ctaggtcaa 
      gcagttgta tcagcatca caactttta attctatgt 
      cttccacca agaatagca gctgtttgg ttcaaaccc 
      aatgaagta tcaactgct tgttctctt ggtgtattt 
      ggaactcaa cttggtgct gccctgggg tttgtgcta 
      tcacccatg cttgtgaaa aatcgtgaa aatgtggaa 
      gaaattgga catgacttg aaaatattg tgctggggt 
      ctcacagct gtcatttta ccaacagcg attgcgatt 
      atattttat tttccaatg caacctcca aagccacca 
      agcattaca caagtggag gaaagaaat agaacggaa 
      aaattttct gcaaaatta ttttttcaa tcaatttca 
      gttctgatg aaaaatatg ccgtttgta atccacgtc 
      atagcttat ggtatcaat attggggtt ttttctgcc 
      attggtact ctcttgaat caacttgtg ttacgctat 
      tttaaacac ttaccagat gctgaagaa tttgctggt 
      agagctgga ttggttatg attgtcatt ggaatgatc 
      gggtcaatt ttatttgga atattcctt gacaagaca 
      cataaatac aaagaaacc acagtttat acttactta 
      ttgtcttca gttagtatt cttgcgttc atgtttgca 
      ttagaaatg aagtcgaaa attttggtt tatattgca 
      ataggagta gttggctta tttacaaat gcctatatg 
      ccagtaggt tttgagttt gcgatggag ttaactttc 
      ccttcttct gaaggtact accacagga ttacttatg 
      gcaccatca caagtttta ggagttgtt tttgcgcta 
      atgttgggt cagcttaac gttgtaatt gggccgttt 
      tgcgtgtct gcaatggaa cacgacatt gtcattgaa 
      aaatttcta ccaaccgaa aacagaaac caagaaatt 
      gttttgtac aaaaaacga tggttaata cttacttta 
      tacatttta tatgccgca attaatgct ttccaatgg 
      tttgaatac tcaataatt gcaaatgtt ttaatgaaa 
      ttttacaat gttagtgcc gtttccgtt gattggact 
      tcaataatt tacatggca ttgtatatg ccaatggtt 
      ataccagta tcatatctc atggagaaa ttggattta 
      cgccaaatt gcagcaatt ggtgttttg ggaaccgca 
      ttaggcaca attgttaaa gtctattca ctttcacca 
      gatcaaatg tgggtgaca tatctgggc caaacaatt 
      ctttcttcc atgcaagtt tttattcta cctctacca 
      cctcgcata gcttcggtt tggtttggt tcgaaagaa 
      gtatcttta gcatgtgca ttgggtgtg tttgggaca 
      cagttaggt caagcgatt ggatttgtt gttcctccg 
      attgttgta cgatacagt gaagatgtg gagacgatt 
      gggcgtgat ttgggtgtt ctagtcaaa ggattagct 
      tggcttacc ttagttgtt actgttgct gttttaaca 
      tatttttcc gctcatccc accactcca ccaaatgat 
      ataaacaac gaaagaacc cattttttt aaatccttg 
      aaaaattta tttaggaat aggagtttt aattttcat 
      actgtcgca tatgggatt aatattgct gtgtttact 
      tcaatttca acgctactg aatcagttt tttttgttt 
      cactttcca aaatcggaa gaagatgct ggaagagtt 
      ggtttaata atggtaatt ttgggcatg atgggagct 
      atagctttc ggttatatt ttagacaag acacaccga 
      tacaaagaa ataacgttg tgcatgtat attgcatcc 
      tttttatca gttattgct tttatgttt tccctcgaa 
      tcgggttca aaaattttg gtctacatt tccggagct 
      ttagtaggc ttattcata aatgcatat atgccagtt 
      ggactagaa ttagcaata gagctgact tatccagaa 
      cctgaaagc acaagttcc ggaattttg atctccatg 
      acccagaca ttgggtgtt ttatttact ttaatgtta 
      ggagaaagt tatgtaaaa gtggaggcg tggcttgat 
      ctcattcac tag
    mRNA sequence
    • >TC032973 RA
      cgtttgtta taatacaga ttacgtgat aacaatgca 
      tatttaaac aagctgctt taaaaaatt aaaagatat 
      cgttttttt gcacaactt aaccaacac tgtatttat 
      aatggaaga tcataataa ggatgaaac aaatttcaa 
      tctactcca agataagcg gttgaaacc aaatggtca 
      cgaaatcaa ggcttacaa acgacgatg gataatttt 
      aacaatttt catagttta cgctgctgt aaactcgta 
      ccaatggat cgaatattc aattatcaa caacataat 
      tacaagata ttacaacgt tactcctgt gatggtgga 
      ctggacatc aatcattta catggccct ctatgcacc 
      tttagtcat accagcttc ctacatttt ggataaata 
      tggtttacg tgccgctgg actaattgg aggactagg 
      aacagcatt aggaacctc aattaaagt attttcaat 
      cggacgtga atccttctg gattgtcct tctaggtca 
      agcagttgt atcagcatc acaactttt aattctatg 
      tcttccacc aagaatagc agctgtttg gttcaaacc 
      caatgaagt atcaactgc ttgttctct tggtgtatt 
      tggaactca acttggtgc tgccctggg gtttgtgct 
      atcacccat gcttgtgaa aaatcgtga aaatgtgga 
      agaaattgg acatgactt gaaaatatt gtgctgggg 
      tctcacagc tgtcatttt accaacagc gattgcgat 
      tatatttta ttttccaat gcaacctcc aaagccacc 
      aagcattac acaagtgga ggaaagaaa tagaacgga 
      aaaattttc tgcaaaatt attttttca atcaatttc 
      agttctgat gaaaaatat gccgtttgt aatccacgt 
      catagctta tggtatcaa tattggggt tttttctgc 
      cattggtac tctcttgaa tcaacttgt gttacgcta 
      ttttaaaca cttaccaga tgctgaaga atttgctgg 
      tagagctgg attggttat gattgtcat tggaatgat 
      cgggtcaat tttatttgg aatattcct tgacaagac 
      acataaata caaagaaac cacagttta tacttactt 
      attgtcttc agttagtat tcttgcgtt catgtttgc 
      attagaaat gaagtcgaa aattttggt ttatattgc 
      aataggagt agttggctt atttacaaa tgcctatat 
      gccagtagg ttttgagtt tgcgatgga gttaacttt 
      cccttcttc tgaaggtac taccacagg attacttat 
      ggcaccatc acaagtttt aggagttgt ttttgcgct 
      aatgttggg tcagcttaa cgttgtaat tgggccgtt 
      ttgcgtgtc tgcaatgga acacgacat tgtcattga 
      aaaatttct accaaccga aaacagaaa ccaagaaat 
      tgttttgta caaaaaacg atggttaat acttacttt 
      atacatttt atatgccgc aattaatgc tttccaatg 
      gtttgaata ctcaataat tgcaaatgt tttaatgaa 
      attttacaa tgttagtgc cgtttccgt tgattggac 
      ttcaataat ttacatggc attgtatat gccaatggt 
      tataccagt atcatatct catggagaa attggattt 
      acgccaaat tgcagcaat tggtgtttt gggaaccgc 
      attaggcac aattgttaa agtctattc actttcacc 
      agatcaaat gtgggtgac atatctggg ccaaacaat 
      tctttcttc catgcaagt ttttattct acctctacc 
      acctcgcat agcttcggt ttggtttgg ttcgaaaga 
      agtatcttt agcatgtgc attgggtgt gtttgggac 
      acagttagg tcaagcgat tggatttgt tgttcctcc 
      gattgttgt acgatacag tgaagatgt ggagacgat 
      tgggcgtga tttgggtgt tctagtcaa aggattagc 
      ttggcttac cttagttgt tactgttgc tgttttaac 
      atatttttc cgctcatcc caccactcc accaaatga 
      tataaacaa cgaaagaac ccatttttt taaatcctt 
      gaaaaattt atttaggaa taggagttt taattttca 
      tactgtcgc atatgggat taatattgc tgtgtttac 
      ttcaatttc aacgctact gaatcagtt ttttttgtt 
      tcactttcc aaaatcgga agaagatgc tggaagagt 
      tggtttaat aatggtaat tttgggcat gatgggagc 
      tatagcttt cggttatat tttagacaa gacacaccg 
      atacaaaga aataacgtt gtgcatgta tattgcatc 
      ctttttatc agttattgc ttttatgtt ttccctcga 
      atcgggttc aaaaatttt ggtctacat ttccggagc 
      tttagtagg cttattcat aaatgcata tatgccagt 
      tggactaga attagcaat agagctgac ttatccaga 
      acctgaaag cacaagttc cggaatttt gatctccat 
      gacccagac attgggtgt tttatttac tttaatgtt 
      aggagaaag ttatgtaaa agtggaggc gtggcttga 
      tctcattca ctagatggc gtagcctgt gctataagc 
      tataagtgc gtatctcct cttgccaaa caagctatc 
      atatggtag gatataaaa tgaataata ctctttgtt 
      ctacatgtg gttgtgaac atgaacaaa agtggtttt 
      tgttacatt ctgtaccta acgtgagtt aacaccgtg 
      ttatagaat gatgtgata agtgaacca gatttgact 
      tttattaat tcaattatg caattattt agttaattg 
      ttctgaaac tactttatt atccgcatt taaaaaatc 
      atgagtgaa caaatatca ggtaggtat ctaataata 
      gtttataat tgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY