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Gene information
General information
TC008584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1516737..1521607
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008584
      iBeetle screen iB_04522

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008584 RA
        MLIRQIIVT LVIALGVLC DTADNKIEE CILPEDLVN 
        EIASYAPTV QKIINTSIN GSFKGITYN ELAYFVDKF 
        GNRISGSQN LEDAIDFMI KKSSASGLD NVHGEEVKV 
        PHWIRGSES ATMIEPRTK SLTLLGLGN SVGTPPEGI 
        LAEVLVVKS FDELNAKAG QAKGKIVVY NQDYISYGQ 
        SVAYRGQGA TAAAKVGGV ASLIRSITP FSLNTPHTG 
        MQSYKDNVT KIPTACITV EDAHLLQRL QDEGYTIKI 
        LLKMEAQTL PDVTSRNVV AEIEGSKQP EKVVIVSGH 
        IDSWDVGDG AMDDGGGVF ISWGALALL KNLNLKPKR 
        TIRSVFWTA EEFGYVGAF GYAQQHQNE TDNFVFLME 
        SDIGTFNPV GLEYAGGAT GYCIVQEIA KLLAPINAT 
        KTRQSPSVG SDIEIWTGN GIPGASLDT ENDKYFWYH 
        HTPADTLDA EDSVSLDKN TALWASVAY VIADLSIDF 
        PRDSLKTKH
      CDS sequence
      • >TC008584 RA
        atgttgatc cgtcaaatt atcgtaact ctagtcata 
        gcattaggg gtgttgtgt gatactgcc gacaataaa 
        attgaagaa tgcattctt ccagaagat ctcgtcaat 
        gagattgct tcatatgca ccaactgta caaaaaatt 
        atcaatact tcaattaat ggaagcttc aagggaatc 
        acttacaat gaactggct tatttcgtc gacaaattc 
        gggaaccgg atttccggc tcccaaaat ttagaagat 
        gcaattgat ttcatgatt aagaaatct agtgcttcc 
        ggcctagac aatgttcat ggcgaagaa gttaaagta 
        cctcattgg ataagaggt tcagaaagt gcaacaatg 
        atagaacca cgtacaaaa agtttgacc cttttgggt 
        ttgggaaac agtgtagga accccaccg gaaggaatt 
        ttagctgaa gttttggtt gttaaatct tttgacgaa 
        ttgaatgca aaagctgga caggcaaag ggaaaaatc 
        gttgtttac aaccaagat tacatcagt tatggccaa 
        tctgttgct tataggggt caaggagct accgctgct 
        gctaaagtc ggtggtgta gcaagttta atccgttcc 
        atcaccccc ttttctcta aacacgcca catacagga 
        atgcaaagt tacaaagat aatgttact aaaattccg 
        acggcttgt attacagta gaagatgca catttatta 
        caaaggttg caagatgaa ggttacacg ataaagatt 
        cttttaaaa atggaagca caaactttg cctgatgta 
        acatccaga aacgttgtg gctgaaatt gaaggcagc 
        aaacaacca gaaaaagtc gtcattgtt tcaggccat 
        atcgatagt tgggatgtt ggtgatggt gcaatggat 
        gatggaggt ggtgttttt atttcatgg ggtgctttg 
        gcacttctt aaaaacctt aacttgaaa cctaaaaga 
        acaatccga agtgtattt tggacggct gaagagttt 
        ggttatgtt ggtgctttt ggatatgca cagcagcat 
        caaaacgaa actgataat tttgttttt cttatggag 
        tccgatatt ggaactttt aatcctgtg ggtctagaa 
        tatgcagga ggagctaca ggttattgc atcgttcaa 
        gaaattgca aagttgtta gcacctatt aacgccact 
        aaaacgaga caatccccc tctgtaggt tctgatatt 
        gaaatttgg accggtaat ggaattcct ggagcgtct 
        cttgatacc gaaaatgat aaatatttt tggtaccac 
        catacacct gctgacact ttagatgca gaagattca 
        gtttcttta gataaaaat actgctctt tgggcgtct 
        gttgcgtat gttatcgct gatttaagt attgatttt 
        cctcgagat tcactcaag actaaacac tga
      mRNA sequence
      • >TC008584 RA
        tttgtgtcg cgatgttga tccgtcaaa ttatcgtaa 
        ctctagtca tagcattag gggtgttgt gtgatactg 
        ccgacaata aaattgaag aatgcattc ttccagaag 
        atctcgtca atgagattg cttcatatg caccaactg 
        tacaaaaaa ttatcaata cttcaatta atggaagct 
        tcaagggaa tcacttaca atgaactgg cttatttcg 
        tcgacaaat tcgggaacc ggatttccg gctcccaaa 
        atttagaag atgcaattg atttcatga ttaagaaat 
        ctagtgctt ccggcctag acaatgttc atggcgaag 
        aagttaaag tacctcatt ggataagag gttcagaaa 
        gtgcaacaa tgatagaac cacgtacaa aaagtttga 
        cccttttgg gtttgggaa acagtgtag gaaccccac 
        cggaaggaa ttttagctg aagttttgg ttgttaaat 
        cttttgacg aattgaatg caaaagctg gacaggcaa 
        agggaaaaa tcgttgttt acaaccaag attacatca 
        gttatggcc aatctgttg cttataggg gtcaaggag 
        ctaccgctg ctgctaaag tcggtggtg tagcaagtt 
        taatccgtt ccatcaccc ccttttctc taaacacgc 
        cacatacag gaatgcaaa gttacaaag ataatgtta 
        ctaaaattc cgacggctt gtattacag tagaagatg 
        cacatttat tacaaaggt tgcaagatg aaggttaca 
        cgataaaga ttcttttaa aaatggaag cacaaactt 
        tgcctgatg taacatcca gaaacgttg tggctgaaa 
        ttgaaggca gcaaacaac cagaaaaag tcgtcattg 
        tttcaggcc atatcgata gttgggatg ttggtgatg 
        gtgcaatgg atgatggag gtggtgttt ttatttcat 
        ggggtgctt tggcacttc ttaaaaacc ttaacttga 
        aacctaaaa gaacaatcc gaagtgtat tttggacgg 
        ctgaagagt ttggttatg ttggtgctt ttggatatg 
        cacagcagc atcaaaacg aaactgata attttgttt 
        ttcttatgg agtccgata ttggaactt ttaatcctg 
        tgggtctag aatatgcag gaggagcta caggttatt 
        gcatcgttc aagaaattg caaagttgt tagcaccta 
        ttaacgcca ctaaaacga gacaatccc cctctgtag 
        gttctgata ttgaaattt ggaccggta atggaattc 
        ctggagcgt ctcttgata ccgaaaatg ataaatatt 
        tttggtacc accatacac ctgctgaca ctttagatg 
        cagaagatt cagtttctt tagataaaa atactgctc 
        tttgggcgt ctgttgcgt atgttatcg ctgatttaa 
        gtattgatt ttcctcgag attcactca agactaaac 
        actgattat aatcaacta tctcaagta tttaaataa 
        aatgattag aatcta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY