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Gene information
General information
TC008532 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451492.1: 195418..199557
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008532
    iBeetle screen iB_04513

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008532 RA
      MEHKKLWGG RFSASTDSN LEKLNSSIT FDKRMYAED 
      IEGSKAYAE ALKKINLLT EHETTQICK GLDRVKIEW 
      DQNLFETKE GDEDIHTAN ERRLKEIIG EPATKLHVG 
      RSRNDQVVT DMKLWLRSH LQDLDKIVK SLIKVIITR 
      SAHEIDIMM PGYTHLQRA QPVRWSHYL LSHAWNLKK 
      DCDRLDDIV KLINIMPLG SGALAGNPF NIDRMSLAS 
      SLDFDSVTQ NSMQAVGDR DFIAEFLFW ASMVGIHLS 
      RLAEDLILY SSKEFEFVT IDDTYSTGS SLMPQKRNP 
      DSLELIRGI GGSLFGQCC SFMMVLKSL PSTYNKDLQ 
      YDKEAMFTT FDKLKSILE VATGTVKTL KVNQDKCKN 
      ALSFEMLAT DVAYYLVRK EVPFRDAHF LAGKVVAEA 
      ERKGVSICD LTLEELKNI SSSFDVDIG RVWNYEHSI 
      EQYKAIGGT AKSSVAKQI ELLEKWLM
    CDS sequence
    • >TC008532 RA
      atggaacac aaaaagtta tggggaggt cgtttttcg 
      gcatcgacc gactcaaat ttagaaaaa ctgaactct 
      tctattact ttcgataaa cggatgtat gctgaagat 
      atcgaaggc agtaaggcg tacgctgaa gctttgaaa 
      aaaattaat ctcttaact gagcatgaa actacacaa 
      atttgcaag ggcctagac cgagttaaa atcgaatgg 
      gaccaaaat ttatttgaa acgaaagaa ggagatgaa 
      gatatacac acggccaat gaacgacgt ctcaaggaa 
      ataattgga gaaccagct accaagctc cacgttggt 
      cgcagcaga aacgaccaa gtggtcacc gacatgaaa 
      ctttggctc cgaagtcat ttacaagac ttggacaaa 
      atcgtgaaa tccttaatc aaagtgatt atcaccaga 
      tctgcacac gaaattgat ataatgatg cctggttat 
      acccacttg caacgagcc caacctgtg cgatggagt 
      cactacttg ttgagccat gcctggaat cttaaaaaa 
      gactgcgac cgattggac gacattgtc aagcttatc 
      aacatcatg cccttagga agtggtgcc ttggctggg 
      aaccctttc aatattgat agaatgagt ttagcttca 
      tcacttgac tttgactct gttacccaa aacagcatg 
      caagcggtg ggcgatcgt gactttatt gctgaattc 
      ttgttttgg gcctcaatg gttggaatt catttaagt 
      cgtctagct gaagatctg attctctac agctctaag 
      gaattcgaa tttgtcaca atcgatgat acctactcc 
      actgggagt agcttaatg ccccaaaaa cgcaatcct 
      gatagtctc gaattaatt cgaggtatt gggggaagt 
      ttatttgga caatgttgt agttttatg atggttctg 
      aaaagttta ccctcgact tacaacaaa gacttgcaa 
      tatgacaag gaagcaatg tttactact ttcgacaaa 
      ctcaagtcc attttagaa gtagccacg ggtacggtc 
      aagactctc aaagtgaac caagataag tgtaaaaat 
      gcgttaagt tttgagatg ttagctacc gacgtggct 
      tattatctg gtgcggaaa gaagtaccg tttagagat 
      gctcatttt ttggctgga aaggtcgta gctgaagcg 
      gaacgtaaa ggagtatcc atttgtgat ttaacgttg 
      gaagaattg aagaacatt agttcatcc tttgacgtg 
      gacattgga cgagtttgg aactatgaa catagcatc 
      gaacaatat aaagctatt ggtgggaca gctaaatca 
      agtgttgcc aaacaaatt gagttactc gaaaaatgg 
      ttgatgtaa
    mRNA sequence
    • >TC008532 RA
      aatatggtc aaaaggtca caagaatct tatttaaaa 
      caatgctgg ggatattac ttacatcct tgattgagt 
      tagttttta aactctata attctcaat gagcgataa 
      gaggcagtg aaattacat aatattatt tcgatattg 
      taaactgtt aatacgagt cataaataa tcaatgaag 
      aaaacaaat taaacagaa tggaacaca aaaagttat 
      ggggaggtc gtttttcgg catcgaccg actcaaatt 
      tagaaaaac tgaactctt ctattactt tcgataaac 
      ggatgtatg ctgaagata tcgaaggca gtaaggcgt 
      acgctgaag ctttgaaaa aaattaatc tcttaactg 
      agcatgaaa ctacacaaa tttgcaagg gcctagacc 
      gagttaaaa tcgaatggg accaaaatt tatttgaaa 
      cgaaagaag gagatgaag atatacaca cggccaatg 
      aacgacgtc tcaaggaaa taattggag aaccagcta 
      ccaagctcc acgttggtc gcagcagaa acgaccaag 
      tggtcaccg acatgaaac tttggctcc gaagtcatt 
      tacaagact tggacaaaa tcgtgaaat ccttaatca 
      aagtgatta tcaccagat ctgcacacg aaattgata 
      taatgatgc ctggttata cccacttgc aacgagccc 
      aacctgtgc gatggagtc actacttgt tgagccatg 
      cctggaatc ttaaaaaag actgcgacc gattggacg 
      acattgtca agcttatca acatcatgc ccttaggaa 
      gtggtgcct tggctggga accctttca atattgata 
      gaatgagtt tagcttcat cacttgact ttgactctg 
      ttacccaaa acagcatgc aagcggtgg gcgatcgtg 
      actttattg ctgaattct tgttttggg cctcaatgg 
      ttggaattc atttaagtc gtctagctg aagatctga 
      ttctctaca gctctaagg aattcgaat ttgtcacaa 
      tcgatgata cctactcca ctgggagta gcttaatgc 
      cccaaaaac gcaatcctg atagtctcg aattaattc 
      gaggtattg ggggaagtt tatttggac aatgttgta 
      gttttatga tggttctga aaagtttac cctcgactt 
      acaacaaag acttgcaat atgacaagg aagcaatgt 
      ttactactt tcgacaaac tcaagtcca ttttagaag 
      tagccacgg gtacggtca agactctca aagtgaacc 
      aagataagt gtaaaaatg cgttaagtt ttgagatgt 
      tagctaccg acgtggctt attatctgg tgcggaaag 
      aagtaccgt ttagagatg ctcattttt tggctggaa 
      aggtcgtag ctgaagcgg aacgtaaag gagtatcca 
      tttgtgatt taacgttgg aagaattga agaacatta 
      gttcatcct ttgacgtgg acattggac gagtttgga 
      actatgaac atagcatcg aacaatata aagctattg 
      gtgggacag ctaaatcaa gtgttgcca aacaaattg 
      agttactcg aaaaatggt tgatgtaat gtttgcaaa 
      ttcataata aacttctgt gtgaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY