Status
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Gene information
General information
TC032960
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008526
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12238367..12264026
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032960
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04511

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032960 RA
      MFESRVVKT DVTAVKLIN NFVLAGIGN FLHIFDLNG 
      KFLKKLNVF DGQKIHGIN LDDVTDTLL LYGGCYMQT 
      ITCNFLQCE FTKLHAKTV HGWVLTSRW LKSKWIAAV 
      VMLNKLLIF DGQLNILRE IVCAEKCIL YSAFISYND 
      IDELVVLSG TVFGEIVIW KVNHFNIQE SSPVVKRLT 
      GHKGVIFSI SYNEEAGYI CSSSDDRST ILWSIEKKS 
      IHSELQRTH CNILLHRKI YGHQSRVFR SLLLQNLIV 
      TAGEDSTVN VWDFEGQLI KKITHQGGP IWALDGNTD 
      VVVSGGGDC GVLISSVQS NSLCNSLLL PENYNPKRI 
      GLLRSSNLV VITEDGVLF YYARNLNMW IKIDQHKVL 
      QSYNVLEVS KCKSLFSLS GYHGEIFIY QEEENSARL 
      LFFCL
    CDS sequence
    • >TC032960 RA
      atgttcgaa tcacgtgtt gtgaaaacg gatgtaacc 
      gctgtgaag ttaattaat aacttcgtt ttagctggt 
      attggtaac tttttacat atttttgac ctcaacgga 
      aaatttcta aaaaaatta aacgtcttt gatggacag 
      aaaatacat ggaataaat cttgatgat gtcactgac 
      acgttacta ttgtatggt ggttgttat atgcagaca 
      ataacttgt aattttcta caatgtgaa tttacaaaa 
      ttgcatgcg aaaactgtc catggatgg gttctaaca 
      agtcggtgg ttgaaaagt aaatggatc gctgctgta 
      gttatgcta aacaagtta ttaattttt gacggccag 
      ttgaatata ctgcgagaa attgtttgt gctgagaaa 
      tgcatcttg tatagtgct ttcatttcc tacaacgac 
      attgacgaa ttggtggtc ttgtctgga acagttttt 
      ggggaaata gtaatttgg aaagtgaat cactttaat 
      attcaggaa agtagtccc gttgtcaaa agactgact 
      ggtcataag ggtgttatt ttttccatt agttacaat 
      gaagaagca ggttatatt tgttcttca tctgacgat 
      cgttctacc attttgtgg tcaatcgag aagaagtca 
      attcacagt gagttgcaa cgtactcat tgtaacatc 
      cttttacat cgcaaaatt tacggtcat cagtctcgt 
      gtattccga tcattatta ctacaaaat ttaatagtg 
      acagctggt gaagattct accgtaaac gtttgggat 
      ttcgaagga caattaata aaaaaaata actcatcaa 
      ggtggacca atttgggca ttagatggt aacactgat 
      gtggtggtg tcaggaggt ggtgattgc ggagtgtta 
      atatcgtca gtacaatca aattcgcta tgtaacagt 
      ttacttctt ccagagaat tataatcct aagcgaatt 
      ggactttta cgcagcagt aatctagtt gtgattacc 
      gaggatggt gtattattt tactatgct cgtaattta 
      aacatgtgg ataaaaatc gatcaacat aaggtgtta 
      caaagttat aacgtgctt gaagtgtca aagtgtaaa 
      agtttgttt tcgctttct gggtaccat ggcgagatt 
      tttatctac caggaagaa gaaaactca gctcgactg 
      ttgtttttt tgtttgtga
    mRNA sequence
    • >TC032960 RA
      ccgttgctg tctgttggc gtatgatgt ggataggga 
      ttgttgttt tatcattta taagtttgt aaatgttcc 
      aaaagtgtt aaatattct attacattt gcaccaaaa 
      atcccgcga ggttgacaa taatcaata ccttattca 
      tttcgtttt caaagcaag atgttcgaa tcacgtgtt 
      gtgaaaacg gatgtaacc gctgtgaag ttaattaat 
      aacttcgtt ttagctggt attggtaac tttttacat 
      atttttgac ctcaacgga aaatttcta aaaaaatta 
      aacgtcttt gatggacag aaaatacat ggaataaat 
      cttgatgat gtcactgac acgttacta ttgtatggt 
      ggttgttat atgcagaca ataacttgt aattttcta 
      caatgtgaa tttacaaaa ttgcatgcg aaaactgtc 
      catggatgg gttctaaca agtcggtgg ttgaaaagt 
      aaatggatc gctgctgta gttatgcta aacaagtta 
      ttaattttt gacggccag ttgaatata ctgcgagaa 
      attgtttgt gctgagaaa tgcatcttg tatagtgct 
      ttcatttcc tacaacgac attgacgaa ttggtggtc 
      ttgtctgga acagttttt ggggaaata gtaatttgg 
      aaagtgaat cactttaat attcaggaa agtagtccc 
      gttgtcaaa agactgact ggtcataag ggtgttatt 
      ttttccatt agttacaat gaagaagca ggttatatt 
      tgttcttca tctgacgat cgttctacc attttgtgg 
      tcaatcgag aagaagtca attcacagt gagttgcaa 
      cgtactcat tgtaacatc cttttacat cgcaaaatt 
      tacggtcat cagtctcgt gtattccga tcattatta 
      ctacaaaat ttaatagtg acagctggt gaagattct 
      accgtaaac gtttgggat ttcgaagga caattaata 
      aaaaaaata actcatcaa ggtggacca atttgggca 
      ttagatggt aacactgat gtggtggtg tcaggaggt 
      ggtgattgc ggagtgtta atatcgtca gtacaatca 
      aattcgcta tgtaacagt ttacttctt ccagagaat 
      tataatcct aagcgaatt ggactttta cgcagcagt 
      aatctagtt gtgattacc gaggatggt gtattattt 
      tactatgct cgtaattta aacatgtgg ataaaaatc 
      gatcaacat aaggtgtta caaagttat aacgtgctt 
      gaagtgtca aagtgtaaa agtttgttt tcgctttct 
      gggtaccat ggcgagatt tttatctac caggaagaa 
      gaaaactca gctcgactg ttgtttttt tgtttgtga 
      atccaaatt atcgctttg gtatctcga tggagcaaa 
      tgttacgtt tgtgacagt ttttgatct tccgagatg 
      taaagacat atggattag cgcagcctg tcaaggtct 
      tcacggaaa atatattgc cggtgatcg tagaggaaa 
      tatttatat ttacaaatt gggatttgc tgcaccaat 
      tttcacata taagaaagc tcacagtta tatgggagt 
      tactcattt atgtaattt tgataatga catagtgtc 
      attgggacg tgatggtac tgttaagac gtttacgat 
      agaaaataa ctctctggt ccataagtc tagtgataa 
      ggttccaac atcgtggct tgcttcagt ggttggtcc 
      ttttctgtt gggtttttc tggaaacgt ttttgtcgt 
      ttgggatta tatcaaaaa aagaatttt atttgagca 
      tgtttgtgg tgggggtca tcgatcgtg ggactttaa 
      caaattaaa taatatgat atcgtttgc gtatgttca 
      aaacggtat tgtgaaaat tcttaattg ggatgaaca 
      tgaagtcta ttctgtagt tgctggctt tcacgccaa 
      tacaattaa cagtgcgag aattttgac aactactga 
      taaaaattt tattgtttt gtcgggcgg agaagatac 
      aaatttacg aatttcgtt gtttggttc aaattattt 
      cttacctgc taagagttt caagtcaca tttgtcttg 
      tattcggtg catttgtct ttacgaatt atataaaaa 
      ccggtattt ggtgtttac tggtggcgg tagaggcca 
      gataatctg ttggaaatt tgttttggt tgataagcc 
      tggcgatag tctttgtag tgaagaatg ttccttcta 
      tgaaaagtt tgacggtga attgtttga atcagaagt 
      acgtatcat ggatttgaa gacactgac tttgggaga 
      agatctgtt attgtttgc tgctgcgtc tgacggaaa 
      tgtaaatat gtttaccgt tgttaagga gaacacaaa 
      atacgtgct tgtcccttt gataaagtt attctacca 
      aaataaatg cgttacgaa actttgttt gatagacgt 
      ttttcaatc taatgtttt aggttcatt cagtacaga 
      tggtacatt ggcgttttg ggatgttac ttgtattga 
      tacaaagtt aggaatttg tccttttaa aattttgtc 
      gcttcacca gtcaggaat tgctgactg ctgttttaa 
      atttgtcaa agaaaattc ttttgtact tttgacggg 
      tggtgatga cgggtcgtt tgtattgac tttgtttca 
      catatcaaa aagtgcagc tgatggcct gaagtttga 
      aaaagtttt ttctttttg cgattgtca ctctcactg 
      cgctcaagt aactggcac ccagatttc gagtaatta 
      ttttataac aacatcaat tgatcagaa attgttact 
      tttcaagtg gactgttga gaaagacaa gcttgtggt 
      gaaagcttg tggtcgcta caattccgc cgttacgga 
      tgttcaggg gctgcaata taaagagaa ggatgatta 
      ttttaatat atttgttta tggtgaagg tttagagtt 
      tattacata taaaaaaaa ataaatact tttgtaatg 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY