| General information | |||
| TC032960
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 12238367..12264026 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032960 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04511 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC032960 RA
MFESRVVKT DVTAVKLIN NFVLAGIGN FLHIFDLNG KFLKKLNVF DGQKIHGIN LDDVTDTLL LYGGCYMQT ITCNFLQCE FTKLHAKTV HGWVLTSRW LKSKWIAAV VMLNKLLIF DGQLNILRE IVCAEKCIL YSAFISYND IDELVVLSG TVFGEIVIW KVNHFNIQE SSPVVKRLT GHKGVIFSI SYNEEAGYI CSSSDDRST ILWSIEKKS IHSELQRTH CNILLHRKI YGHQSRVFR SLLLQNLIV TAGEDSTVN VWDFEGQLI KKITHQGGP IWALDGNTD VVVSGGGDC GVLISSVQS NSLCNSLLL PENYNPKRI GLLRSSNLV VITEDGVLF YYARNLNMW IKIDQHKVL QSYNVLEVS KCKSLFSLS GYHGEIFIY QEEENSARL LFFCL
- >TC032960 RA
atgttcgaa tcacgtgtt gtgaaaacg gatgtaacc gctgtgaag ttaattaat aacttcgtt ttagctggt attggtaac tttttacat atttttgac ctcaacgga aaatttcta aaaaaatta aacgtcttt gatggacag aaaatacat ggaataaat cttgatgat gtcactgac acgttacta ttgtatggt ggttgttat atgcagaca ataacttgt aattttcta caatgtgaa tttacaaaa ttgcatgcg aaaactgtc catggatgg gttctaaca agtcggtgg ttgaaaagt aaatggatc gctgctgta gttatgcta aacaagtta ttaattttt gacggccag ttgaatata ctgcgagaa attgtttgt gctgagaaa tgcatcttg tatagtgct ttcatttcc tacaacgac attgacgaa ttggtggtc ttgtctgga acagttttt ggggaaata gtaatttgg aaagtgaat cactttaat attcaggaa agtagtccc gttgtcaaa agactgact ggtcataag ggtgttatt ttttccatt agttacaat gaagaagca ggttatatt tgttcttca tctgacgat cgttctacc attttgtgg tcaatcgag aagaagtca attcacagt gagttgcaa cgtactcat tgtaacatc cttttacat cgcaaaatt tacggtcat cagtctcgt gtattccga tcattatta ctacaaaat ttaatagtg acagctggt gaagattct accgtaaac gtttgggat ttcgaagga caattaata aaaaaaata actcatcaa ggtggacca atttgggca ttagatggt aacactgat gtggtggtg tcaggaggt ggtgattgc ggagtgtta atatcgtca gtacaatca aattcgcta tgtaacagt ttacttctt ccagagaat tataatcct aagcgaatt ggactttta cgcagcagt aatctagtt gtgattacc gaggatggt gtattattt tactatgct cgtaattta aacatgtgg ataaaaatc gatcaacat aaggtgtta caaagttat aacgtgctt gaagtgtca aagtgtaaa agtttgttt tcgctttct gggtaccat ggcgagatt tttatctac caggaagaa gaaaactca gctcgactg ttgtttttt tgtttgtga
- >TC032960 RA
ccgttgctg tctgttggc gtatgatgt ggataggga ttgttgttt tatcattta taagtttgt aaatgttcc aaaagtgtt aaatattct attacattt gcaccaaaa atcccgcga ggttgacaa taatcaata ccttattca tttcgtttt caaagcaag atgttcgaa tcacgtgtt gtgaaaacg gatgtaacc gctgtgaag ttaattaat aacttcgtt ttagctggt attggtaac tttttacat atttttgac ctcaacgga aaatttcta aaaaaatta aacgtcttt gatggacag aaaatacat ggaataaat cttgatgat gtcactgac acgttacta ttgtatggt ggttgttat atgcagaca ataacttgt aattttcta caatgtgaa tttacaaaa ttgcatgcg aaaactgtc catggatgg gttctaaca agtcggtgg ttgaaaagt aaatggatc gctgctgta gttatgcta aacaagtta ttaattttt gacggccag ttgaatata ctgcgagaa attgtttgt gctgagaaa tgcatcttg tatagtgct ttcatttcc tacaacgac attgacgaa ttggtggtc ttgtctgga acagttttt ggggaaata gtaatttgg aaagtgaat cactttaat attcaggaa agtagtccc gttgtcaaa agactgact ggtcataag ggtgttatt ttttccatt agttacaat gaagaagca ggttatatt tgttcttca tctgacgat cgttctacc attttgtgg tcaatcgag aagaagtca attcacagt gagttgcaa cgtactcat tgtaacatc cttttacat cgcaaaatt tacggtcat cagtctcgt gtattccga tcattatta ctacaaaat ttaatagtg acagctggt gaagattct accgtaaac gtttgggat ttcgaagga caattaata aaaaaaata actcatcaa ggtggacca atttgggca ttagatggt aacactgat gtggtggtg tcaggaggt ggtgattgc ggagtgtta atatcgtca gtacaatca aattcgcta tgtaacagt ttacttctt ccagagaat tataatcct aagcgaatt ggactttta cgcagcagt aatctagtt gtgattacc gaggatggt gtattattt tactatgct cgtaattta aacatgtgg ataaaaatc gatcaacat aaggtgtta caaagttat aacgtgctt gaagtgtca aagtgtaaa agtttgttt tcgctttct gggtaccat ggcgagatt tttatctac caggaagaa gaaaactca gctcgactg ttgtttttt tgtttgtga atccaaatt atcgctttg gtatctcga tggagcaaa tgttacgtt tgtgacagt ttttgatct tccgagatg taaagacat atggattag cgcagcctg tcaaggtct tcacggaaa atatattgc cggtgatcg tagaggaaa tatttatat ttacaaatt gggatttgc tgcaccaat tttcacata taagaaagc tcacagtta tatgggagt tactcattt atgtaattt tgataatga catagtgtc attgggacg tgatggtac tgttaagac gtttacgat agaaaataa ctctctggt ccataagtc tagtgataa ggttccaac atcgtggct tgcttcagt ggttggtcc ttttctgtt gggtttttc tggaaacgt ttttgtcgt ttgggatta tatcaaaaa aagaatttt atttgagca tgtttgtgg tgggggtca tcgatcgtg ggactttaa caaattaaa taatatgat atcgtttgc gtatgttca aaacggtat tgtgaaaat tcttaattg ggatgaaca tgaagtcta ttctgtagt tgctggctt tcacgccaa tacaattaa cagtgcgag aattttgac aactactga taaaaattt tattgtttt gtcgggcgg agaagatac aaatttacg aatttcgtt gtttggttc aaattattt cttacctgc taagagttt caagtcaca tttgtcttg tattcggtg catttgtct ttacgaatt atataaaaa ccggtattt ggtgtttac tggtggcgg tagaggcca gataatctg ttggaaatt tgttttggt tgataagcc tggcgatag tctttgtag tgaagaatg ttccttcta tgaaaagtt tgacggtga attgtttga atcagaagt acgtatcat ggatttgaa gacactgac tttgggaga agatctgtt attgtttgc tgctgcgtc tgacggaaa tgtaaatat gtttaccgt tgttaagga gaacacaaa atacgtgct tgtcccttt gataaagtt attctacca aaataaatg cgttacgaa actttgttt gatagacgt ttttcaatc taatgtttt aggttcatt cagtacaga tggtacatt ggcgttttg ggatgttac ttgtattga tacaaagtt aggaatttg tccttttaa aattttgtc gcttcacca gtcaggaat tgctgactg ctgttttaa atttgtcaa agaaaattc ttttgtact tttgacggg tggtgatga cgggtcgtt tgtattgac tttgtttca catatcaaa aagtgcagc tgatggcct gaagtttga aaaagtttt ttctttttg cgattgtca ctctcactg cgctcaagt aactggcac ccagatttc gagtaatta ttttataac aacatcaat tgatcagaa attgttact tttcaagtg gactgttga gaaagacaa gcttgtggt gaaagcttg tggtcgcta caattccgc cgttacgga tgttcaggg gctgcaata taaagagaa ggatgatta ttttaatat atttgttta tggtgaagg tttagagtt tattacata taaaaaaaa ataaatact tttgtaatg a
