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Gene information
General information
TC032960
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008526 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12238367..12264026
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032960
    iBeetle screen iB_04511

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032960 RA
      MFESRVVKT DVTAVKLIN NFVLAGIGN FLHIFDLNG 
KFLKKLNVF DGQKIHGIN LDDVTDTLL LYGGCYMQT 
ITCNFLQCE FTKLHAKTV HGWVLTSRW LKSKWIAAV 
VMLNKLLIF DGQLNILRE IVCAEKCIL YSAFISYND 
IDELVVLSG TVFGEIVIW KVNHFNIQE SSPVVKRLT 
GHKGVIFSI SYNEEAGYI CSSSDDRST ILWSIEKKS 
IHSELQRTH CNILLHRKI YGHQSRVFR SLLLQNLIV 
TAGEDSTVN VWDFEGQLI KKITHQGGP IWALDGNTD 
VVVSGGGDC GVLISSVQS NSLCNSLLL PENYNPKRI 
GLLRSSNLV VITEDGVLF YYARNLNMW IKIDQHKVL 
QSYNVLEVS KCKSLFSLS GYHGEIFIY QEEENSARL 
LFFCL
    CDS sequence
    • >TC032960 RA
      atgttcgaa tcacgtgtt gtgaaaacg gatgtaacc 
gctgtgaag ttaattaat aacttcgtt ttagctggt 
attggtaac tttttacat atttttgac ctcaacgga 
aaatttcta aaaaaatta aacgtcttt gatggacag 
aaaatacat ggaataaat cttgatgat gtcactgac 
acgttacta ttgtatggt ggttgttat atgcagaca 
ataacttgt aattttcta caatgtgaa tttacaaaa 
ttgcatgcg aaaactgtc catggatgg gttctaaca 
agtcggtgg ttgaaaagt aaatggatc gctgctgta 
gttatgcta aacaagtta ttaattttt gacggccag 
ttgaatata ctgcgagaa attgtttgt gctgagaaa 
tgcatcttg tatagtgct ttcatttcc tacaacgac 
attgacgaa ttggtggtc ttgtctgga acagttttt 
ggggaaata gtaatttgg aaagtgaat cactttaat 
attcaggaa agtagtccc gttgtcaaa agactgact 
ggtcataag ggtgttatt ttttccatt agttacaat 
gaagaagca ggttatatt tgttcttca tctgacgat 
cgttctacc attttgtgg tcaatcgag aagaagtca 
attcacagt gagttgcaa cgtactcat tgtaacatc 
cttttacat cgcaaaatt tacggtcat cagtctcgt 
gtattccga tcattatta ctacaaaat ttaatagtg 
acagctggt gaagattct accgtaaac gtttgggat 
ttcgaagga caattaata aaaaaaata actcatcaa 
ggtggacca atttgggca ttagatggt aacactgat 
gtggtggtg tcaggaggt ggtgattgc ggagtgtta 
atatcgtca gtacaatca aattcgcta tgtaacagt 
ttacttctt ccagagaat tataatcct aagcgaatt 
ggactttta cgcagcagt aatctagtt gtgattacc 
gaggatggt gtattattt tactatgct cgtaattta 
aacatgtgg ataaaaatc gatcaacat aaggtgtta 
caaagttat aacgtgctt gaagtgtca aagtgtaaa 
agtttgttt tcgctttct gggtaccat ggcgagatt 
tttatctac caggaagaa gaaaactca gctcgactg 
ttgtttttt tgtttgtga
    mRNA sequence
    • >TC032960 RA
      ccgttgctg tctgttggc gtatgatgt ggataggga 
ttgttgttt tatcattta taagtttgt aaatgttcc 
aaaagtgtt aaatattct attacattt gcaccaaaa 
atcccgcga ggttgacaa taatcaata ccttattca 
tttcgtttt caaagcaag atgttcgaa tcacgtgtt 
gtgaaaacg gatgtaacc gctgtgaag ttaattaat 
aacttcgtt ttagctggt attggtaac tttttacat 
atttttgac ctcaacgga aaatttcta aaaaaatta 
aacgtcttt gatggacag aaaatacat ggaataaat 
cttgatgat gtcactgac acgttacta ttgtatggt 
ggttgttat atgcagaca ataacttgt aattttcta 
caatgtgaa tttacaaaa ttgcatgcg aaaactgtc 
catggatgg gttctaaca agtcggtgg ttgaaaagt 
aaatggatc gctgctgta gttatgcta aacaagtta 
ttaattttt gacggccag ttgaatata ctgcgagaa 
attgtttgt gctgagaaa tgcatcttg tatagtgct 
ttcatttcc tacaacgac attgacgaa ttggtggtc 
ttgtctgga acagttttt ggggaaata gtaatttgg 
aaagtgaat cactttaat attcaggaa agtagtccc 
gttgtcaaa agactgact ggtcataag ggtgttatt 
ttttccatt agttacaat gaagaagca ggttatatt 
tgttcttca tctgacgat cgttctacc attttgtgg 
tcaatcgag aagaagtca attcacagt gagttgcaa 
cgtactcat tgtaacatc cttttacat cgcaaaatt 
tacggtcat cagtctcgt gtattccga tcattatta 
ctacaaaat ttaatagtg acagctggt gaagattct 
accgtaaac gtttgggat ttcgaagga caattaata 
aaaaaaata actcatcaa ggtggacca atttgggca 
ttagatggt aacactgat gtggtggtg tcaggaggt 
ggtgattgc ggagtgtta atatcgtca gtacaatca 
aattcgcta tgtaacagt ttacttctt ccagagaat 
tataatcct aagcgaatt ggactttta cgcagcagt 
aatctagtt gtgattacc gaggatggt gtattattt 
tactatgct cgtaattta aacatgtgg ataaaaatc 
gatcaacat aaggtgtta caaagttat aacgtgctt 
gaagtgtca aagtgtaaa agtttgttt tcgctttct 
gggtaccat ggcgagatt tttatctac caggaagaa 
gaaaactca gctcgactg ttgtttttt tgtttgtga 
atccaaatt atcgctttg gtatctcga tggagcaaa 
tgttacgtt tgtgacagt ttttgatct tccgagatg 
taaagacat atggattag cgcagcctg tcaaggtct 
tcacggaaa atatattgc cggtgatcg tagaggaaa 
tatttatat ttacaaatt gggatttgc tgcaccaat 
tttcacata taagaaagc tcacagtta tatgggagt 
tactcattt atgtaattt tgataatga catagtgtc 
attgggacg tgatggtac tgttaagac gtttacgat 
agaaaataa ctctctggt ccataagtc tagtgataa 
ggttccaac atcgtggct tgcttcagt ggttggtcc 
ttttctgtt gggtttttc tggaaacgt ttttgtcgt 
ttgggatta tatcaaaaa aagaatttt atttgagca 
tgtttgtgg tgggggtca tcgatcgtg ggactttaa 
caaattaaa taatatgat atcgtttgc gtatgttca 
aaacggtat tgtgaaaat tcttaattg ggatgaaca 
tgaagtcta ttctgtagt tgctggctt tcacgccaa 
tacaattaa cagtgcgag aattttgac aactactga 
taaaaattt tattgtttt gtcgggcgg agaagatac 
aaatttacg aatttcgtt gtttggttc aaattattt 
cttacctgc taagagttt caagtcaca tttgtcttg 
tattcggtg catttgtct ttacgaatt atataaaaa 
ccggtattt ggtgtttac tggtggcgg tagaggcca 
gataatctg ttggaaatt tgttttggt tgataagcc 
tggcgatag tctttgtag tgaagaatg ttccttcta 
tgaaaagtt tgacggtga attgtttga atcagaagt 
acgtatcat ggatttgaa gacactgac tttgggaga 
agatctgtt attgtttgc tgctgcgtc tgacggaaa 
tgtaaatat gtttaccgt tgttaagga gaacacaaa 
atacgtgct tgtcccttt gataaagtt attctacca 
aaataaatg cgttacgaa actttgttt gatagacgt 
ttttcaatc taatgtttt aggttcatt cagtacaga 
tggtacatt ggcgttttg ggatgttac ttgtattga 
tacaaagtt aggaatttg tccttttaa aattttgtc 
gcttcacca gtcaggaat tgctgactg ctgttttaa 
atttgtcaa agaaaattc ttttgtact tttgacggg 
tggtgatga cgggtcgtt tgtattgac tttgtttca 
catatcaaa aagtgcagc tgatggcct gaagtttga 
aaaagtttt ttctttttg cgattgtca ctctcactg 
cgctcaagt aactggcac ccagatttc gagtaatta 
ttttataac aacatcaat tgatcagaa attgttact 
tttcaagtg gactgttga gaaagacaa gcttgtggt 
gaaagcttg tggtcgcta caattccgc cgttacgga 
tgttcaggg gctgcaata taaagagaa ggatgatta 
ttttaatat atttgttta tggtgaagg tttagagtt 
tattacata taaaaaaaa ataaatact tttgtaatg 
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    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY