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Gene information
General information
TC008521 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12128752..12153795
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008521
    iBeetle screen iB_04509

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: 15 of 29 (51,72%)
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008521 RA
      MDTILRRIN VVGWHRMLV VFLFIWLVI LVFTALPML 
      GPHIPSGET KTLERLKRA LTDLEALRK QNNELQEIF 
      KDINVDSLR GDQKEAIEN FQYRLTKAE HTFSKNQVG 
      YVSAKEEPN SEYELLRRR IYSNTKEFW YFIHSGLLD 
      LEKKAHESA PEIVDSVKY LLSLGVEHK RSLLHDIDQ 
      LAEVDGYAT WREKEANDL SGLVQKRFH FLQNPADCK 
      TAKKLICSL NKGCGYGCQ LHHAVYCFM VAYGTKRTL 
      ILKSKGWRY HKAGWEEIF KPVSDTCVD PSGKSVSNW 
      PGNSETQVV NLPIIDSLS PRPPFLPLA IPEDLAPRL 
      TRLHGDPIV WWVGQILKF LLRPQPQTA AVMQETMTH 
      MGFKRPIVG VHVRRTDKV GTEAAYHGI EEYMTAVDE 
      YYKELELKT SVDKRRIYL ATDDPKVIA DAKSKYSHY 
      EILGDPSIS KTAAISTRY SDSSLFGII NDINMLAMS 
      DYLVCTFSS QVCRVAYEI MQTYYPDAS SRFRSLDDV 
      YYYGGQNPH NTIAVLSHE PRKNGEMSV VAGDLIGVA 
      GNHWNGFSK GRNLRTNQI ALYPSFKVK DKIETAKFP 
      TYAEVDKHE NDDQQIS
    • >TC008521 RB
      MLGPHIPSG ETKTLERLK RALTDLEAL RKQNNELQE 
      IFKDINVDS LRGDQKEAI ENFQYRLTK AEHTFSKNQ 
      VGYVSAKEE PNSEYELLR RRIYSNTKE FWYFIHSGL 
      LDLEKKAHE SAPEIVDSV KYLLSLGVE HKRSLLHDI 
      DQLAEVDGY ATWREKEAN DLSGLVQKR FHFLQNPAD 
      CKTAKKLIC SLNKGCGYG CQLHHAVYC FMVAYGTKR 
      TLILKSKGW RYHKAGWEE IFKPVSDTC VDPSGKSVS 
      NWPGNSETQ VVNLPIIDS LSPRPPFLP LAIPEDLAP 
      RLTRLHGDP IVWWVGQIL KFLLRPQPQ TAAVMQETM 
      THMGFKRPI VGVHVRRTD KVGTEAAYH GIEEYMTAV 
      DEYYKELEL KTSVDKRRI YLATDDPKV IADAKSKYS 
      HYEILGDPS ISKTAAIST RYSDSSLFG IINDINMLA 
      MSDYLVCTF SSQVCRVAY EIMQTYYPD ASSRFRSLD 
      DVYYYGGQN PHNTIAVLS HEPRKNGEM SVVAGDLIG 
      VAGNHWNGF SKGRNLRTN QIALYPSFK VKDKIETAK 
      FPTYAEVDK HENDDQQIS
    CDS sequence
    • >TC008521 RA
      atggatacg atattaaga agaataaat gtcgtcgga 
      tggcatcga atgctagtt gtttttctt tttatttgg 
      ctggttata cttgttttc acggcactg cctatgcta 
      ggacctcac ataccctca ggagagacc aaaactttg 
      gagcgctta aaaagagcg ttgacggat ttggaagcc 
      ctccggaaa caaaataat gaactacag gaaattttt 
      aaagacatt aatgtcgat tcgttgcgt ggagaccag 
      aaggaagcg atcgaaaac tttcaatac cgtttaaca 
      aaggccgaa cacacattt agcaaaaat caagtaggt 
      tatgtgagt gcgaaagaa gagcccaac tcagagtat 
      gagctttta agacgccga atttatagc aataccaaa 
      gaattttgg tattttatt cattcaggt ttgttggat 
      cttgaaaaa aaagctcat gaatcggct ccggaaata 
      gtcgattct gttaaatat ttactgagt ttgggtgtt 
      gaacataaa aggtctttg ttgcatgat attgatcag 
      ttggctgaa gtcgatggt tatgcgaca tggagggaa 
      aaagaagcg aatgacttg agtggactt gtacagaag 
      aggtttcat tttttgcaa aatccggcc gattgcaaa 
      acagccaaa aaacttatt tgcagtttg aataagggt 
      tgtggatac ggttgtcaa ttacaccac gccgtgtat 
      tgttttatg gtagcttat ggaacaaag cgcactctg 
      attttgaaa tcaaaaggt tggcgttat cataaagct 
      ggatgggaa gaaatattt aaacctgtt agtgacact 
      tgtgttgat cctagtgga aaatcggtc agcaactgg 
      cctggcaac tctgagact caagttgtt aatttacca 
      ataatagat tcgttatca ccacgtcca cctttctta 
      ccactagcc attccagaa gatttagct ccgcgattg 
      actcgactt catggagat ccaattgtt tggtgggtg 
      gggcaaatt ttaaagttt cttcttagg ccacagcct 
      caaactgca gcagttatg caagaaacg atgacccat 
      atggggttc aagaggcct attgtgggt gttcatgtt 
      cgtagaacg gataaggtt ggcaccgaa gctgcctat 
      cacggtatc gaagaatat atgactgct gttgacgaa 
      tactataaa gagttggaa cttaaaaca agtgttgat 
      aaaagaaga atctattta gctactgac gacccaaaa 
      gtaattgcg gatgctaaa agtaaatat tcccactat 
      gaaattttg ggtgatcct tcgatttcg aaaacagcg 
      gcaatttca acaagatat tccgattcg tcattgttc 
      ggtattatc aatgatatt aacatgttg gctatgagt 
      gattatctg gtttgcacg ttttcgtct caggtttgt 
      cgagtggcc tacgaaata atgcagact tattacccc 
      gatgcttca tcccgtttt cgctcacta gatgacgtc 
      tactattac ggtggtcaa aatcctcac aataccatc 
      gctgtgtta tcgcacgag ccgagaaaa aacggtgaa 
      atgagtgtt gtcgcaggc gacttgatc ggtgtggct 
      ggtaatcat tggaacggt ttcagtaaa ggtcggaat 
      ttacgaact aatcaaatc gcattatat cccagtttt 
      aaagttaaa gacaaaatt gaaacagcc aaatttcct 
      acgtatgca gaagtagac aaacatgaa aatgacgac 
      caacaaatt tcttag
    • >TC008521 RB
      atgctagga cctcacata ccctcagga gagaccaaa 
      actttggag cgcttaaaa agagcgttg acggatttg 
      gaagccctc cggaaacaa aataatgaa ctacaggaa 
      atttttaaa gacattaat gtcgattcg ttgcgtgga 
      gaccagaag gaagcgatc gaaaacttt caataccgt 
      ttaacaaag gccgaacac acatttagc aaaaatcaa 
      gtaggttat gtgagtgcg aaagaagag cccaactca 
      gagtatgag cttttaaga cgccgaatt tatagcaat 
      accaaagaa ttttggtat tttattcat tcaggtttg 
      ttggatctt gaaaaaaaa gctcatgaa tcggctccg 
      gaaatagtc gattctgtt aaatattta ctgagtttg 
      ggtgttgaa cataaaagg tctttgttg catgatatt 
      gatcagttg gctgaagtc gatggttat gcgacatgg 
      agggaaaaa gaagcgaat gacttgagt ggacttgta 
      cagaagagg tttcatttt ttgcaaaat ccggccgat 
      tgcaaaaca gccaaaaaa cttatttgc agtttgaat 
      aagggttgt ggatacggt tgtcaatta caccacgcc 
      gtgtattgt tttatggta gcttatgga acaaagcgc 
      actctgatt ttgaaatca aaaggttgg cgttatcat 
      aaagctgga tgggaagaa atatttaaa cctgttagt 
      gacacttgt gttgatcct agtggaaaa tcggtcagc 
      aactggcct ggcaactct gagactcaa gttgttaat 
      ttaccaata atagattcg ttatcacca cgtccacct 
      ttcttacca ctagccatt ccagaagat ttagctccg 
      cgattgact cgacttcat ggagatcca attgtttgg 
      tgggtgggg caaatttta aagtttctt cttaggcca 
      cagcctcaa actgcagca gttatgcaa gaaacgatg 
      acccatatg gggttcaag aggcctatt gtgggtgtt 
      catgttcgt agaacggat aaggttggc accgaagct 
      gcctatcac ggtatcgaa gaatatatg actgctgtt 
      gacgaatac tataaagag ttggaactt aaaacaagt 
      gttgataaa agaagaatc tatttagct actgacgac 
      ccaaaagta attgcggat gctaaaagt aaatattcc 
      cactatgaa attttgggt gatccttcg atttcgaaa 
      acagcggca atttcaaca agatattcc gattcgtca 
      ttgttcggt attatcaat gatattaac atgttggct 
      atgagtgat tatctggtt tgcacgttt tcgtctcag 
      gtttgtcga gtggcctac gaaataatg cagacttat 
      taccccgat gcttcatcc cgttttcgc tcactagat 
      gacgtctac tattacggt ggtcaaaat cctcacaat 
      accatcgct gtgttatcg cacgagccg agaaaaaac 
      ggtgaaatg agtgttgtc gcaggcgac ttgatcggt 
      gtggctggt aatcattgg aacggtttc agtaaaggt 
      cggaattta cgaactaat caaatcgca ttatatccc 
      agttttaaa gttaaagac aaaattgaa acagccaaa 
      tttcctacg tatgcagaa gtagacaaa catgaaaat 
      gacgaccaa caaatttct tag
    mRNA sequence
    • >TC008521 RA
      ttgtactta gaaaacaac tgctgtcag attgttgag 
      tgttttatt gttgttaaa acaaattga cacgtattt 
      ctgtcgtgg tgttgttac gtacgtttt tccaatagt 
      ttacataaa taaacaaat aatggatat cgtgagacg 
      aatctgtgc aaggtttaa ttgaataca attcagata 
      tggaaaagt gtaacttaa cctacaaat aagcgatgg 
      atacgatat taagaagaa taaatgtcg tcggatggc 
      atcgaatgc tagttgttt ttcttttta tttggctgg 
      ttatacttg ttttcacgg cactgccta tgctaggac 
      ctcacatac cctcaggag agaccaaaa ctttggagc 
      gcttaaaaa gagcgttga cggatttgg aagccctcc 
      ggaaacaaa ataatgaac tacaggaaa tttttaaag 
      acattaatg tcgattcgt tgcgtggag accagaagg 
      aagcgatcg aaaactttc aataccgtt taacaaagg 
      ccgaacaca catttagca aaaatcaag taggttatg 
      tgagtgcga aagaagagc ccaactcag agtatgagc 
      ttttaagac gccgaattt atagcaata ccaaagaat 
      tttggtatt ttattcatt caggtttgt tggatcttg 
      aaaaaaaag ctcatgaat cggctccgg aaatagtcg 
      attctgtta aatatttac tgagtttgg gtgttgaac 
      ataaaaggt ctttgttgc atgatattg atcagttgg 
      ctgaagtcg atggttatg cgacatgga gggaaaaag 
      aagcgaatg acttgagtg gacttgtac agaagaggt 
      ttcattttt tgcaaaatc cggccgatt gcaaaacag 
      ccaaaaaac ttatttgca gtttgaata agggttgtg 
      gatacggtt gtcaattac accacgccg tgtattgtt 
      ttatggtag cttatggaa caaagcgca ctctgattt 
      tgaaatcaa aaggttggc gttatcata aagctggat 
      gggaagaaa tatttaaac ctgttagtg acacttgtg 
      ttgatccta gtggaaaat cggtcagca actggcctg 
      gcaactctg agactcaag ttgttaatt taccaataa 
      tagattcgt tatcaccac gtccacctt tcttaccac 
      tagccattc cagaagatt tagctccgc gattgactc 
      gacttcatg gagatccaa ttgtttggt gggtggggc 
      aaattttaa agtttcttc ttaggccac agcctcaaa 
      ctgcagcag ttatgcaag aaacgatga cccatatgg 
      ggttcaaga ggcctattg tgggtgttc atgttcgta 
      gaacggata aggttggca ccgaagctg cctatcacg 
      gtatcgaag aatatatga ctgctgttg acgaatact 
      ataaagagt tggaactta aaacaagtg ttgataaaa 
      gaagaatct atttagcta ctgacgacc caaaagtaa 
      ttgcggatg ctaaaagta aatattccc actatgaaa 
      ttttgggtg atccttcga tttcgaaaa cagcggcaa 
      tttcaacaa gatattccg attcgtcat tgttcggta 
      ttatcaatg atattaaca tgttggcta tgagtgatt 
      atctggttt gcacgtttt cgtctcagg tttgtcgag 
      tggcctacg aaataatgc agacttatt accccgatg 
      cttcatccc gttttcgct cactagatg acgtctact 
      attacggtg gtcaaaatc ctcacaata ccatcgctg 
      tgttatcgc acgagccga gaaaaaacg gtgaaatga 
      gtgttgtcg caggcgact tgatcggtg tggctggta 
      atcattgga acggtttca gtaaaggtc ggaatttac 
      gaactaatc aaatcgcat tatatccca gttttaaag 
      ttaaagaca aaattgaaa cagccaaat ttcctacgt 
      atgcagaag tagacaaac atgaaaatg acgaccaac 
      aaatttctt agttgacca ttattgttg tgataaatt 
      tatttttga ctgtaagcc tctatacta gtctgagta 
      aaaatattg ttattataa gttttctat ttttagttg 
      acttttttg gtaattttt gctggtgaa tcaactgaa 
      acaattttt ggattttaa aagtgagtt ggcaatact 
      actaaattt tatttttaa cttaacaaa ctgcaaata 
      ttttacatt caagataag atacaaaat gaacaaaaa 
      gtatgaaaa catgcgttt tctgattaa agtgattaa 
      aaattaaaa acaaaattt ttgtttaca caaattata 
      gtatctgag ttattttta caacgttgg caggtttgt 
      gtcttgtta aagttgatt cattcttat acacgaaca 
      tttgaaatg attactttt ttctgcaag ggaacaaat 
      aacgattaa gtggtgcat ttagttgca ttttttctc 
      gtacaaatt gaggtagtg ttaaccgtc actttgtat 
      acaaatata catattttt tttggaacc aatctctta 
      agtatatac ggggtgttt taaaattct agtcagctg 
      tgtttctcg tttttcagg tcatttgat ttattggga 
      aaaaatgtt cttgttggc gacatgata ataattaat 
      ttagaatac actatataa taaatcatt tatcattgt 
      tattgaaac acagaaatg gaaaatttg cgcgaaatt 
      ttaaattat aattttcaa atcttaaaa aaagtagcc 
      gaaaaatca ttaacttaa aaactttct ttttatcaa 
      aacaagtta ctaccctac caaatttgt caagacttg 
      aaactgaaa taaatatta aaaataata agattaaat 
      ttgaaatta aaaatacaa ttttttcgc tttttaaag 
      aatttgtaa aatgattat tgattatca cacttttgg 
      ctaagtttg tacttgtgt atttagtat catttgaaa 
      ttaccgccc aaactgcag ttccgtcta ctgtcaggt 
      ttatatgaa aattggtgg gaaatttaa acgtaaaag 
      tgtttacat aatttttgc acttgttta gttgcaaag 
      tgaaatttt gctcatttt acaaaaaaa attcttaat 
      tgtttttat gtttgaaaa aatcgaaga gtattagtg 
      caatagaag aaaataaaa cgaaaaacg agcttatga 
      aacattgtt tcaaatgta gaaaatggg tataagtgt 
      tataattgt ttgttccta cattttatt atgtatgtt 
      aaaaaataa acgtgtaga aatactact tgctacgtc 
      catcagagt aacattgtt caacatatc tttattttt 
      aaattatta ttttatcca agacagtaa atggaaaac 
      gttacgccc tctgttggt cgattttgt agtgatttt 
      aaaattggc caccagatt gcgtaataa ttcttctta 
      tatcatata ataaaatta tgtgccaaa agcattagt 
      ttcattttt tagaaaatt taaaatgtg caaatgtaa 
      gtagaagat atgttttta tgcacaatg ttattatag 
      catataaat caggacatg tagaaattg tacaaatat 
      ataaatcag gcacctgag gtcttcatt tcttcaaca 
      aaaaaaaga gatttttgg ttgtaagta ctctgagga 
      agagcactt tgttattat tagttaata tttgtaata 
      aaatatttt cacagtg
    • >TC008521 RB
      ttgtactta gaaaacaac tgctgtcag attgttgag 
      tgttttatt gttgttaaa acaaattga cacgtattt 
      ctgtcgtgg tgttgttac gtacgtttt tccaatagt 
      ttacataaa taaacaaat aatggatat cgtgagacg 
      aatctgtgc aaggtttaa ttgaataca attcagata 
      tggaaaagt gtaacttaa cctacaaat aagcgatgg 
      atacgatat taagaagaa taaatgtcg tcggatggc 
      atcgaatgc tagttgttt ttcttttta tttggctgg 
      ttatacttg ttttcacgg cactgccta tgctaggac 
      ctcacatac cctcaggag agaccaaaa ctttggagc 
      gcttaaaaa gagcgttga cggatttgg aagccctcc 
      ggaaacaaa ataatgaac tacaggaaa tttttaaag 
      acattaatg tcgattcgt tgcgtggag accagaagg 
      aagcgatcg aaaactttc aataccgtt taacaaagg 
      ccgaacaca catttagca aaaatcaag taggttatg 
      tgagtgcga aagaagagc ccaactcag agtatgagc 
      ttttaagac gccgaattt atagcaata ccaaagaat 
      tttggtatt ttattcatt caggtttgt tggatcttg 
      aaaaaaaag ctcatgaat cggctccgg aaatagtcg 
      attctgtta aatatttac tgagtttgg gtgttgaac 
      ataaaaggt ctttgttgc atgatattg atcagttgg 
      ctgaagtcg atggttatg cgacatgga gggaaaaag 
      aagcgaatg acttgagtg gacttgtac agaagaggt 
      ttcattttt tgcaaaatc cggccgatt gcaaaacag 
      ccaaaaaac ttatttgca gtttgaata agggttgtg 
      gatacggtt gtcaattac accacgccg tgtattgtt 
      ttatggtag cttatggaa caaagcgca ctctgattt 
      tgaaatcaa aaggttggc gttatcata aagctggat 
      gggaagaaa tatttaaac ctgttagtg acacttgtg 
      ttgatccta gtggaaaat cggtcagca actggcctg 
      gcaactctg agactcaag ttgttaatt taccaataa 
      tagattcgt tatcaccac gtccacctt tcttaccac 
      tagccattc cagaagatt tagctccgc gattgactc 
      gacttcatg gagatccaa ttgtttggt gggtggggc 
      aaattttaa agtttcttc ttaggccac agcctcaaa 
      ctgcagcag ttatgcaag aaacgatga cccatatgg 
      ggttcaaga ggcctattg tgggtgttc atgttcgta 
      gaacggata aggttggca ccgaagctg cctatcacg 
      gtatcgaag aatatatga ctgctgttg acgaatact 
      ataaagagt tggaactta aaacaagtg ttgataaaa 
      gaagaatct atttagcta ctgacgacc caaaagtaa 
      ttgcggatg ctaaaagta aatattccc actatgaaa 
      ttttgggtg atccttcga tttcgaaaa cagcggcaa 
      tttcaacaa gatattccg attcgtcat tgttcggta 
      ttatcaatg atattaaca tgttggcta tgagtgatt 
      atctggttt gcacgtttt cgtctcagg tttgtcgag 
      tggcctacg aaataatgc agacttatt accccgatg 
      cttcatccc gttttcgct cactagatg acgtctact 
      attacggtg gtcaaaatc ctcacaata ccatcgctg 
      tgttatcgc acgagccga gaaaaaacg gtgaaatga 
      gtgttgtcg caggcgact tgatcggtg tggctggta 
      atcattgga acggtttca gtaaaggtc ggaatttac 
      gaactaatc aaatcgcat tatatccca gttttaaag 
      ttaaagaca aaattgaaa cagccaaat ttcctacgt 
      atgcagaag tagacaaac atgaaaatg acgaccaac 
      aaatttctt agttgacca ttattgttg tgataaatt 
      tatttttga ctgtaagcc tctatacta gtctgagta 
      aaaatattg ttattataa gttttctat ttttagttg 
      acttttttg gtaattttt gctggtgaa tcaactgaa 
      acaattttt ggattttaa aagtgagtt ggcaatact 
      actaaattt tatttttaa cttaacaaa ctgcaaata 
      ttttacatt caagataag atacaaaat gaacaaaaa 
      gtatgaaaa catgcgttt tctgattaa agtgattaa 
      aaattaaaa acaaaattt ttgtttaca caaattata 
      gtatctgag ttattttta caacgttgg caggtttgt 
      gtcttgtta aagttgatt cattcttat acacgaaca 
      tttgaaatg attactttt ttctgcaag ggaacaaat 
      aacgattaa gtggtgcat ttagttgca ttttttctc 
      gtacaaatt gaggtagtg ttaaccgtc actttgtat 
      acaaatata catattttt tttggaacc aatctctta 
      agtatatac ggggtgttt taaaattct agtcagctg 
      tgtttctcg tttttcagg tcatttgat ttattggga 
      aaaaatgtt cttgttggc gacatgata ataattaat 
      ttagaatac actatataa taaatcatt tatcattgt 
      tattgaaac acagaaatg gaaaatttg cgcgaaatt 
      ttaaattat aattttcaa atcttaaaa aaagtagcc 
      gaaaaatca ttaacttaa aaactttct ttttatcaa 
      aacaagtta ctaccctac caaatttgt caagacttg 
      aaactgaaa taaatatta aaaataata agattaaat 
      ttgaaatta aaaatacaa ttttttcgc tttttaaag 
      aatttgtaa aatgattat tgattatca cacttttgg 
      ctaagtttg tacttgtgt atttagtat catttgaaa 
      ttaccgccc aaactgcag ttccgtcta ctgtcaggt 
      ttatatgaa aattggtgg gaaatttaa acgtaaaag 
      tgtttacat aatttttgc acttgttta gttgcaaag 
      tgaaatttt gctcatttt acaaaaaaa attcttaat 
      tgtttttat gtttgaaaa aatcgaaga gtattagtg 
      caatagaag aaaataaaa cgaaaaacg agcttatga 
      aacattgtt tcaaatgta gaaaatggg tataagtgt 
      tataattgt ttgttccta cattttatt atgtatgtt 
      aaaaaataa acgtgtaga aatactact tgctacgtc 
      catcagagt aacattgtt caacatatc tttattttt 
      aaattatta ttttatcca agacagtaa atggaaaac 
      gttacgccc tctgttggt cgattttgt agtgatttt 
      aaaattggc caccagatt gcgtaataa ttcttctta 
      tatcatata ataaaatta tgtgccaaa agcattagt 
      ttcattttt tagaaaatt taaaatgtg caaatgtaa 
      gtagaagat atgttttta tgcacaatg ttattatag 
      catataaat caggacatg tagaaattg tacaaatat 
      ataaatcag gcacctgag gtcttcatt tcttcaaca 
      aaaaaaaga gatttttgg ttgtaagta ctctgagga 
      agagcactt tgttattat tagttaata tttgtaata 
      aaatatttt cacagtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY