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Gene information
General information
TC008516 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12043503..12049124
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008516
    iBeetle screen iB_04508

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008516 RA
      MTTNSQNMG HSTINMLLT MIAFIVVVL SLSLAYLKE 
      GPSNLSCSF AYYTLYLLF TAFLAVLPF GGPKVPKDT 
      NRQEFHVLT GGLSLVSCL LVLFTLEVF GVKILNFIT 
      GNLFDLTIS ALLTGLFLT FYVHLRSYF LPNECLNPK 
      VIGKNAIES LFLGREVLL DCAFLCENT KNKTLLVLS 
      TIKFVYFID GLLMEYTYE TSMEVRQVG FGFGCGVGN 
      FVYPFLMSV LTKYQISTN LQFENWRLF LTCVVFTIG 
      YLIYRVSNN QKYNFRENP GAKGFKSIP TGVKNKKLL 
      CSGLWGIVR HPNYLGDIL VHWSFTGFM LCVPGFMIY 
      FDILFMIDR SSRDNMNCK EKYGKVWDQ YCDEVKYIL 
      VPWIY
    • >TC008516 RB
      MTTNSQNMG HSTINMLLT MIAFIVVVL SLSLAYLKE 
      GPSNLSCSF AYYTLYLLF TAFLAVLPF GGPKVPKDT 
      NRQEFHVLT GGLSLVSCL LVLFTLEVF GVKILNFIT 
      GNLFDLTIS ALLTGLFLT FYVHLRSYF LPNECLNPK 
      VIGKNAIES LFLGREVLL DCAFLCENT KNKTLLVLS 
      TIKFVYFID GLLMEYTYE TSMEVRQVG FGFGCGVGN 
      FVYPFLMSV LTKYQISTN LQFENWRLF LTCVVFTIG 
      YLIYRVSNN QKYNFRENP GAKGFMIYF DILFMIDRS 
      SRDNMNCKE KYGKVWDQY CDEVKYILV PWIY
    CDS sequence
    • >TC008516 RA
      atgacaaca aactcacaa aatatgggt cattctact 
      atcaacatg ttattaacc atgattgcg tttatcgta 
      gtcgtatta tccttatct ctagcttat ctcaaggaa 
      ggaccatca aacttatca tgtagtttt gcctactac 
      accctgtac ctacttttc acagccttc ttagcagtg 
      ttgccattc ggaggcccg aaagtgcca aaagacaca 
      aaccgacaa gaatttcat gttttaaca ggaggtctt 
      agtttagtt tcttgtttg ctcgtttta ttcactttg 
      gaagtgttt ggcgtcaaa attctaaat tttattact 
      ggaaatttg tttgatttg acaatttct gcacttttg 
      acggggctt ttcctcaca ttttatgtc catttgagg 
      agctatttt ttaccgaat gagtgtttg aatcctaaa 
      gtaattggg aaaaatgca attgaaagt cttttcctt 
      ggacgagag gtgctactt gattgtgcg tttctttgc 
      gaaaataca aaaaataaa accttgctt gttttgagt 
      acaattaag tttgtttac tttatcgat ggtctgttg 
      atggaatac acttacgaa acttcgatg gaagtgcga 
      caagttggt tttgggttt ggttgtggg gttggtaat 
      tttgtttac ccatttttg atgagtgta ttgacaaaa 
      taccaaatc tccaccaat cttcaattt gagaattgg 
      cgacttttt cttacttgt gtcgttttc acaattggt 
      tatctcatc tatcgtgtt agcaacaat cagaagtac 
      aattttagg gaaaatcct ggggcaaaa ggtttcaaa 
      tcgattccg actggtgtt aaaaacaag aaactgcta 
      tgttcagga ttgtgggga attgtacgt catcccaat 
      tatttgggg gatatttta gttcactgg tcgttcact 
      ggctttatg ttgtgtgtg ccaggattt atgatctat 
      ttcgatatt ttgtttatg attgatcgt tcaagtcgg 
      gataatatg aattgtaag gagaaatat ggtaaagtt 
      tgggaccaa tattgcgat gaagtgaaa tatattctt 
      gtaccttgg atttattaa
    • >TC008516 RB
      atgacaaca aactcacaa aatatgggt cattctact 
      atcaacatg ttattaacc atgattgcg tttatcgta 
      gtcgtatta tccttatct ctagcttat ctcaaggaa 
      ggaccatca aacttatca tgtagtttt gcctactac 
      accctgtac ctacttttc acagccttc ttagcagtg 
      ttgccattc ggaggcccg aaagtgcca aaagacaca 
      aaccgacaa gaatttcat gttttaaca ggaggtctt 
      agtttagtt tcttgtttg ctcgtttta ttcactttg 
      gaagtgttt ggcgtcaaa attctaaat tttattact 
      ggaaatttg tttgatttg acaatttct gcacttttg 
      acggggctt ttcctcaca ttttatgtc catttgagg 
      agctatttt ttaccgaat gagtgtttg aatcctaaa 
      gtaattggg aaaaatgca attgaaagt cttttcctt 
      ggacgagag gtgctactt gattgtgcg tttctttgc 
      gaaaataca aaaaataaa accttgctt gttttgagt 
      acaattaag tttgtttac tttatcgat ggtctgttg 
      atggaatac acttacgaa acttcgatg gaagtgcga 
      caagttggt tttgggttt ggttgtggg gttggtaat 
      tttgtttac ccatttttg atgagtgta ttgacaaaa 
      taccaaatc tccaccaat cttcaattt gagaattgg 
      cgacttttt cttacttgt gtcgttttc acaattggt 
      tatctcatc tatcgtgtt agcaacaat cagaagtac 
      aattttagg gaaaatcct ggggcaaaa ggatttatg 
      atctatttc gatattttg tttatgatt gatcgttca 
      agtcgggat aatatgaat tgtaaggag aaatatggt 
      aaagtttgg gaccaatat tgcgatgaa gtgaaatat 
      attcttgta ccttggatt tattaa
    mRNA sequence
    • >TC008516 RA
      tgaatatta aatttttac aataacaag tagcaaagt 
      ataaggaaa cctttctca caatgacaa caaactcac 
      aaaatatgg gtcattcta ctatcaaca tgttattaa 
      ccatgattg cgtttatcg tagtcgtat tatccttat 
      ctctagctt atctcaagg aaggaccat caaacttat 
      catgtagtt ttgcctact acaccctgt acctacttt 
      tcacagcct tcttagcag tgttgccat tcggaggcc 
      cgaaagtgc caaaagaca caaaccgac aagaatttc 
      atgttttaa caggaggtc ttagtttag tttcttgtt 
      tgctcgttt tattcactt tggaagtgt ttggcgtca 
      aaattctaa attttatta ctggaaatt tgtttgatt 
      tgacaattt ctgcacttt tgacggggc ttttcctca 
      cattttatg tccatttga ggagctatt ttttaccga 
      atgagtgtt tgaatccta aagtaattg ggaaaaatg 
      caattgaaa gtcttttcc ttggacgag aggtgctac 
      ttgattgtg cgtttcttt gcgaaaata caaaaaata 
      aaaccttgc ttgttttga gtacaatta agtttgttt 
      actttatcg atggtctgt tgatggaat acacttacg 
      aaacttcga tggaagtgc gacaagttg gttttgggt 
      ttggttgtg gggttggta attttgttt acccatttt 
      tgatgagtg tattgacaa aataccaaa tctccacca 
      atcttcaat ttgagaatt ggcgacttt ttcttactt 
      gtgtcgttt tcacaattg gttatctca tctatcgtg 
      ttagcaaca atcagaagt acaatttta gggaaaatc 
      ctggggcaa aaggtttca aatcgattc cgactggtg 
      ttaaaaaca agaaactgc tatgttcag gattgtggg 
      gaattgtac gtcatccca attatttgg gggatattt 
      tagttcact ggtcgttca ctggcttta tgttgtgtg 
      tgccaggat ttatgatct atttcgata ttttgttta 
      tgattgatc gttcaagtc gggataata tgaattgta 
      aggagaaat atggtaaag tttgggacc aatattgcg 
      atgaagtga aatatattc ttgtacctt ggatttatt 
      aaagaataa acaattttt caatgt
    • >TC008516 RB
      tgaatatta aatttttac aataacaag tagcaaagt 
      ataaggaaa cctttctca caatgacaa caaactcac 
      aaaatatgg gtcattcta ctatcaaca tgttattaa 
      ccatgattg cgtttatcg tagtcgtat tatccttat 
      ctctagctt atctcaagg aaggaccat caaacttat 
      catgtagtt ttgcctact acaccctgt acctacttt 
      tcacagcct tcttagcag tgttgccat tcggaggcc 
      cgaaagtgc caaaagaca caaaccgac aagaatttc 
      atgttttaa caggaggtc ttagtttag tttcttgtt 
      tgctcgttt tattcactt tggaagtgt ttggcgtca 
      aaattctaa attttatta ctggaaatt tgtttgatt 
      tgacaattt ctgcacttt tgacggggc ttttcctca 
      cattttatg tccatttga ggagctatt ttttaccga 
      atgagtgtt tgaatccta aagtaattg ggaaaaatg 
      caattgaaa gtcttttcc ttggacgag aggtgctac 
      ttgattgtg cgtttcttt gcgaaaata caaaaaata 
      aaaccttgc ttgttttga gtacaatta agtttgttt 
      actttatcg atggtctgt tgatggaat acacttacg 
      aaacttcga tggaagtgc gacaagttg gttttgggt 
      ttggttgtg gggttggta attttgttt acccatttt 
      tgatgagtg tattgacaa aataccaaa tctccacca 
      atcttcaat ttgagaatt ggcgacttt ttcttactt 
      gtgtcgttt tcacaattg gttatctca tctatcgtg 
      ttagcaaca atcagaagt acaatttta gggaaaatc 
      ctggggcaa aaggattta tgatctatt tcgatattt 
      tgtttatga ttgatcgtt caagtcggg ataatatga 
      attgtaagg agaaatatg gtaaagttt gggaccaat 
      attgcgatg aagtgaaat atattcttg taccttgga 
      tttattaaa gaataaaca atttttcaa tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY