| General information | |||
| TC032951
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 11536994..11580264 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032951 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04504 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
Fertility
egg production: number no/almost no eggs -
mature egg not present - Number of animals showing the phenotype: 3
mature egg not present - Number of animals showing the phenotype: 3
- >TC032951 RA
MHCGMFGLS SMVSRGLYT YLHKTGRDG CLGIHEHQE YRGFYGHVF SRLIPNSTS GGTITIAGS VSMDGKCTG TSYTDNGIT WDDVVVTAS VKITVQEYE ANLKLDQDE ISLRGVACS FLTGYCIDA VIGETVWNP VALPDCDKY SLIFCGKGH IIRQVNDPQ ATSYLVVEQ GDKVFALSL MKPDAVCGM RVWQTEHPK LLVTETEPE LISRNWKQQ VTTENIDLP AYINSKFLY MEQSLKTSV DRAYAYTFH RRCLLRREI LKNRLVLAP LSPNVVSTL MSGNNKRKQ TRGESPINA RRVRGSTDG SDQTVSSID RLTEVMADF LERSNNRPT AFATKEEVV PPFNPEDRD QSAEAWCRK VDELRGIFR WTEDATIYY ALAKLRGLA EVWYKGLPS VNMSWAEWK EKLRTAFPS TRDFNDDLS RMMQRQKRR DESYARYFY EKLSLINAC RIIGTDAVS CLIGGIDDV VVKNGRESR QSPNPGIAF RIPRDDQRH KSAPETSYS DHFVVLTSV PGGKNPSPR KTIRRETAF GAGLLRLRK RRALSPTVP EASTGKQPE IGAAMQLLS WYRSHGEIL QQEKQRPED GSTTFELTA DRATATVRA HVVPNSVQE VPVLIGRNF TEMPHIVIV KDQTSLNII ATDAESTLS KITPEPALS KIKLRVAQN TIIPQNHAG HIAVSGDEY EGDVLIEAA IRPQEGHEY CLPRTIIHV KANDTAFLP CINLSDNNL NFRKGQTIA RAWPCIEEE PPTEMVLRV NNSPPTDLP LDQVKVGPI NENERDRLF RLLMDYRDC FALTIDELG CAKSAEMEI RLSEDKPFT YRPYRMARS EQDTVKDIV QELLDNNIV RESESNYCS PVLLVKKKN GEQRLCIDY RKLNAQTVK DNHPLPRVD DQIDRLQGG VYFTSLDLR SGYHQIPLS EESKKYTSF VTPFGQYEY NRVPFGLTN APRTFQRFM NKILKPARE NAAVYLDDV LLHAKDVNE ALQNLQKVF EILRSEGLT LNLKKCSFL MTSVTFLGF DIEDGKIKP GQDKTKAIE AYTAPKSVH QIRQFIGLT GYFRHFVKD YALIAKPLT NLTKKSTAW NWGDKEEEA FRKLQAALV TRPTLVLFN AELETGVHT DASALGLAG ILLQKQPDE RLHPVAYYS RQTTDTEQK YHSYELETL AVVESLKKF RSYLLGIKF TVVTDCNAL RATSVKKQI IPRIARWWL QLQEFTFEV KYRPGNRMK HVDALSRNP VESGEIGEV LHIEEADWV LSGQLTDDK IKRIRQILS KPPTNDEER KIYKNYALR DGRVYRITV RGIQWVVPR GMRHQVVRA AHDDFGHFA VEKTLSRLC EHYWFPRMR KYVEQYIAC CIACLYNKR ASGRKEGFL HPIKKTAEP LNVFHVDHL GPFPKSRRG HTHIIAGID AFTKFVFLR AVKSTKVKY VTEYFRDIF ATYGKPKIL ISDQGSCFT SKRFKQFCR QNNIRHVMN AVATPRANG QVERLNRTI LAALLSSTL EEERWDEHI RNVQFAINN VANKSTRKT PSQLLLGYV PRNGTDVVL RDEITQVSS VVEDLVATR EEALAKMSQ AQDNQKKNF DKRRKRPRR YKQGDLVLV EKNVASNTG TSRKLIPPY SGPMVVKTV LPNDRYVVT DMKGSHRTS KKTNYERVV AVDRMRPWR VPGGVSDDT DSESGEDEI VPSDEDPEV TDEVRGSTD GSDQTVSSI DRLTEVMAD FLERSNNRP TAFATKEEV VPPFNPEDR DQSAEAWCR KVDELRGIF RWTEDATIY YALAKLRGL AEVWYKGLP SVNMSWAEW KEKLRTAFP STRDFNDDL SRMMQRQKR RDESYARYF YEKLSLINA CRIIGTDAV SCLIGGIDD VVVKNGRES RQSPNPGIA FRIPRDDQR HKSAPETSY SDHFVVLTS TPGSKNPSP RKTIRRETV FGAGLLRLR KRRTLSPTV PEASTGKQP EIGAAMQLL SCKTRDPAN QKYYKSILI NGVESTAYV DLGSSCTTI RLAEVQKLG LDHDSNQKT ALKAFGNGV VATLGSTTF ELTADRATA TVRAHVVPN SVQEVPVLI GRNFTEMPH IVIVKDQTS LNIIATDAE STLSKITPE PALSKIKLR VAQNTIIPQ NHAGHIAVS GDEYEGDVL IEAAIRPQE GHEYCLPRT IIHVKANDT AFLPCINLS DNNLNFRKG QTIARAWPC IEEEPPTEM VLRVNNSPP TDLPLDQVK VGPINENER DRLFRLLMD YRDCFALTI DELGCAKSA EMEIRLSED KPFTYRPYR MARSEQDTV KDIVQELLD NNIVRESES NYCSPVLLV KKKNGEQRL CIDYRKLNA QTVKDNHPL PRVDDQIDR LQGGVYFTS LDLRSGYHQ IPLSEESKK YTSFVTPFG QYEYNRVPF GLTNAPRTF QRFMNKILK PARENAAVY LDDVFLHAK DVNEALQNL QKVFEILRS EGLTLNLKK CSFLMTSVT FLRFDIEDG KIKPGQDKT KAIEAYTAP KSVHQIRQF IGLTGYFRH FVKDYALIA KPLTNLTKK STAWNWGDK EEEAFRKLQ AALVTRPTL VLFNAELET EVHTDASAL GLAGILLQK QPDERLHPV AYYSRQTTD TEQKYHSYE LETLAVVES LKKFRSYLL GIKFTVVTD CNALRATSV KKQIIPRIA RWWLQLQEF TFEVKYRPG NRMKHVDAL SRNPVESGE IGEVLHIEE ADWVLSGQL TDDKIKRIR QILSKPPTN DEERKIYKN YALRDGRVY RITVRGIQW VVPRGMRHQ VVRAAHDDF GHFAVEKTL SRLCEHYWF PRMRKYVEQ YIACCIACL YNKRASGRK EGFLHPIKK TAEPLNVFH VDHLGPFPK SRRGHTHII AGIDAFTKF VFLRAVKST KVKYVTEYF RDIFATYGK PEILISDQG SCFTSKRFK QFCRQNNIR HVMNAVATP RANGQVERL NRTILAALL SSTLEEERW DEHIRNVQF AINNVANKS TRKTPSQLL LGYVPRNGT DVVLRDEIT QVSSVVEDL VATREEALA KMSQAQDNQ KKNFDKRRK RPRRYKQGD LVLVEKNVA SNTGTSRKL IPPYSGPMV VKTVLPNDR YVVTDMKGS HRTSKKTNY ERVVAVDRM RPWRVPGGV SDDTDSESG EDEIVPSDE DPEVTDDSD KKNSDVSDA EEESRGSVS ELQFTAEEL NAHPEVLSK SQIPSIKKK KDETVAKMV QKYKSLYGK ETTGDVNPP VTKIPGAKS CGILKENQN PEVLEPILA PLQAGTSAN TLESRKIRR NKKVDKL
- >TC032951 RA
atgcactgt ggaatgttt ggtctctcc agcatggtt tcacgcggc ttatacacc tatctccat aagacagga cgagatggc tgtcttggc atccatgaa catcaagaa tatcgcgga ttctatgga catgtcttc tcaaggtta atacctaat agtaccagt ggaggaaca attacaata gccggaagt gttagcatg gatggaaaa tgtactggg actagttac accgacaat ggaataact tgggacgac gtggttgtt acggcttca gtcaaaata actgttcaa gaatatgaa gccaacttg aagctggat caggacgaa ataagtttg agaggagtc gcttgttca ttcctgaca ggatactgt atcgacgct gtcattgga gaaactgtc tggaatcca gtagcgtta ccagactgt gataaatat tcattgata ttttgcgga aaaggacac ataattcgt caggtgaac gaccctcaa gcaacaagc tacttagtg gtggaacag ggcgataaa gtatttgca ttatcactc atgaaaccc gacgcggtg tgtggaatg cgcgtttgg caaactgaa catcctaaa ttattagtg acagaaacc gaaccggaa ctcatttca cgaaattgg aaacaacaa gtgaccacc gagaacatc gatttacca gcttatatc aattcgaaa ttcctctac atggaacaa tcactgaaa acttcagtt gaccgtgcc tatgcttac accttccat cgaagatgt cttcttaga cgagaaatc ttgaagaat cgattagtg ttggcacct ttatctcca aatgtagtc agcacattg atgagcgga aataataag aggaagcaa actcgtggc gaatcgcca attaacgcg cgaagggtt agaggcagc accgacggg tctgatcaa accgtctct tcgatcgac cgcctcacc gaagtgatg gcagatttt ctcgagcgg tcaaacaac cgccctacc gcgtttgcc acaaaggag gaggtggta ccacccttc aacccagag gaccgcgac caaagcgcc gaggcctgg tgtcgcaag gtggacgag ttaaggggt attttccgt tggacggaa gatgccaca atttattat gctttggcg aaattgcgg ggactcgcc gaggtgtgg tacaaaggg ttaccctcc gtaaacatg tcttgggcg gaatggaag gaaaaatta aggactgcg ttcccatcc actcgcgat ttcaatgac gatctctcg agaatgatg cagcggcag aaacgtcgg gatgaaagc tacgctcgg tacttttat gaaaagctt tcgctcata aacgcttgc cgcatcata ggaaccgac gccgtttca tgcttaatc gggggcatt gacgacgtc gttgtaaaa aacgggcgc gagagcagg caatcacca aaccccgga atcgctttt cgcatacct cgcgacgat cagcgacac aaaagcgct cccgagacc agctacagc gaccacttc gtcgttctc acgagcgtc ccaggtggc aaaaaccca tcaccgagg aagacaatt cgacgagaa acggccttc ggtgccggt ttgctacga ttgcggaaa agaagggca ctatcgccg acagtgccg gaagccagc accggaaaa cagccggaa atcggagct gcgatgcaa ctactgtcg tggtaccgg tcacacgga gaaatattg caacaagaa aaacaaaga cccgaagac gggtctacg accttcgag ctaacagct gatcgggca acggcaacc gttcgggcc cacgtggtc ccaaattct gtccaggaa gtacctgtc ctaatcggt cgcaatttc accgagatg ccccacatt gtcatcgta aaggaccag acctcgtta aatataatc gcaaccgac gcagaatca acactgtca aaaatcaca cctgaaccg gcattatca aaaatcaaa cttcgagtc gcgcaaaac accatcatc ccgcaaaat cacgccggt catattgca gtttccgga gacgagtat gagggtgat gtcctcatt gaagctgcg ataagacct caagaaggt catgaatac tgtctaccg cgtacaatt attcacgtc aaggcaaat gataccgcc tttttacca tgtatcaac ctatcggac aacaattta aactttaga aagggacag acaattgca agggcctgg ccctgcatt gaagaagaa ccacccact gagatggtc ttgcgggta aataactca cccccaacc gatctaccg ctcgatcag gtaaaagtc gggcccatc aatgagaac gaaagggat cgccttttt cgattattg atggattat cgagattgt tttgcactc acgatagat gaactcgga tgtgcaaaa tcggcggaa atggaaata aggctctcc gaggataaa cccttcact tatcggccg tatcgaatg gcgagatcg gagcaggac acagtcaaa gatattgtg caagagctt ttggacaat aatatcgtc agagaaagt gagtcaaat tactgctca ccggtatta ttagtcaaa aagaaaaat ggcgaacaa agattgtgc atcgattac cggaagtta aatgcacaa acggttaag gacaaccat cccctacct cgggtggac gatcaaatt gatcggctg cagggtggc gtgtatttc acgagtctc gatttgcgg tcgggttac catcaaatc ccgctaagt gaagagtcg aagaagtat acttcattt gtaaccccc ttcggtcaa tatgagtac aacagagtg ccattcgga ctgacgaat gcgccgcga acgtttcag cgattcatg aataagatc ttgaaacca gcgcgagaa aacgccgcc gtgtatttg gatgacgtc cttttacac gcaaaagac gtcaacgaa gcgttacaa aatctacaa aaagtgttc gagattctc agaagcgaa ggattaaca cttaacttg aaaaagtgt tcgtttctg atgacctcc gtgacattt ttgggattt gatatcgag gacggaaaa atcaaaccc ggtcaagat aaaaccaaa gccatagag gcatatacc gctccgaaa tcagtacac cagattaga caattcatc gggttgacg ggatatttc cgtcacttc gtcaaagat tacgcgttg atcgcgaaa cctttgaca aatctgacg aagaaaagt accgcctgg aattggggc gacaaagag gaagaggcg ttccgcaaa ttacaggcg gcacttgtg acgcgcccg acgttagta ttatttaat gccgagcta gagaccgga gttcacacc gacgccagt gcgttaggg ctggccgga attttattg caaaaacaa cccgacgag cgattacat ccagtggcg tactacagc cggcaaacg accgatact gagcaaaaa tatcattcg tacgagctc gagacacta gccgtcgtg gaaagctta aagaaattt cgatcttac ctgctcggt ataaaattt accgtcgtg accgactgt aatgcatta cgagcgacg agcgttaaa aaacaaata atcccgcgc attgccaga tggtggctc caactgcaa gaatttaca tttgaggtg aagtacaga cccggaaat cgtatgaaa catgtcgat gcgttaagc agaaatccg gtggaatcg ggagaaatc ggcgaagta cttcacatc gaagaggcg gattgggtc ctatcgggg caactaacc gacgacaaa ataaagcgc atccgccaa attttgtcg aaacccccg acaaatgac gaagaacgg aaaatttat aaaaattac gccctacgc gatggacgc gtgtatcgc attaccgtg cggggaatc caatgggtt gtccctaga gggatgaga caccaggtg gtgcgagcc gcacacgac gatttcggt cattttgcg gtggaaaag acattgtcc cgactgtgc gagcattat tggttccct aggatgcgt aagtacgta gagcaatac atcgcttgc tgcattgcg tgtttgtac aataagcgc gcatcgggt agaaaagaa gggtttctt catcccatc aagaagacc gcggaaccg ctaaatgtg ttccatgtt gatcattta ggaccattc cccaaaagt cgtcgaggt cacacgcac atcatcgcc ggaatcgac gcgtttaca aaattcgtt tttctccgt gcagtcaag tcgaccaaa gtaaaatac gtaaccgaa tatttccgt gatattttc gccacgtat ggcaaacca aagatttta atatcagat caaggaagc tgttttact tcaaaacgc ttcaaacaa ttttgccga caaaataat attcgtcac gtgatgaac gcagtcgcg acgccaagg gcgaacggc caagttgag cgcctaaat cggacgatt ttagcggcc cttttatct tcgaccctt gaggaggaa cggtgggac gagcacatc aggaatgtg cagttcgca attaataac gttgcaaat aagtccacc cggaaaaca ccatcccaa ttgttactg ggatatgta ccgcgcaac ggaactgat gtcgtactt cgcgatgaa atcacccaa gtatcttcc gttgtagag gatttagta gctactcga gaggaagca ctcgcaaag atgtcccag gcacaagat aatcagaaa aagaatttt gacaagcga cgaaaacga ccacggcgc tacaaacag ggtgaccta gtgttggtg gaaaagaac gtcgcttca aacacaggt accagccga aaattaata cccccttac agcggcccg atggtggta aaaacagta ttacctaat gatcgctac gtcgtaacc gacatgaaa gggtcacac agaacttcg aaaaagacc aattacgaa cgcgtagtt gctgtcgat cggatgcga ccatggcgc gtgccagga ggagtgtca gatgacacc gacagtgaa agtggggaa gacgagatt gtcccatct gacgaggac ccggaagtg acagacgag gttagaggc agcaccgac gggtctgat caaaccgtc tcttcgatc gaccgcctc accgaagtg atggcagat tttctcgag cggtcaaac aaccgccct accgcgttt gccacaaag gaggaggtg gtaccaccc ttcaaccca gaggaccgc gaccaaagc gccgaggcc tggtgtcgc aaggtggac gagttaagg ggtattttc cgttggacg gaagatgcc acaatttat tatgctttg gcgaaattg cggggactc gccgaggtg tggtacaaa gggttaccc tccgtaaac atgtcttgg gcggaatgg aaggaaaaa ttaaggact gcgttccca tccactcgc gatttcaat gacgatctc tcgagaatg atgcagcgg cagaaacgt cgggatgaa agctacgct cggtacttt tatgaaaag ctttcgctc ataaacgct tgccgcatc ataggaacc gacgccgtt tcatgctta atcgggggc attgacgac gtcgttgta aaaaacggg cgcgagagc aggcaatca ccaaacccc ggaatcgct tttcgcata cctcgcgac gatcagcga cacaaaagc gctcccgaa accagctac agcgaccac ttcgtcgtt ctcacgagc accccaggt agcaaaaac ccatcaccg aggaagaca attcgacga gaaacggtc ttcggtgcc ggtttgcta cgattgcgg aaaagaagg acactatcg ccgacagtg ccggaagcc agcaccgga aaacagccg gaaatcgga gctgcgatg caactactg tcgtgcaaa acgcgcgat ccggctaat caaaaatat tacaaatca atcttaata aacggtgta gaatcaacg gcgtatgta gatttaggt agttcatgt accacaata cgtttagcg gaggttcaa aaattaggg ttggatcac gattccaat caaaaaacc gcactaaaa gcttttggg aacggggtt gttgcaacg ttagggtct acgaccttc gagctaaca gctgatcgg gcaacggca accgttcgg gcccacgtg gtcccaaat tctgtccag gaagtacct gtcctaatc ggtcgcaat ttcaccgag atgccccac attgtcatc gtaaaggac cagacctcg ttaaatata atcgcaacc gacgcagaa tcaacactg tcaaaaatc acacctgaa ccggcatta tcaaaaatc aaacttcga gtcgcgcaa aacaccatc atcccgcaa aatcacgcc ggtcatatt gcagtttcc ggagacgag tatgagggt gatgtcctc attgaagct gcgataaga cctcaagaa ggtcatgaa tactgtcta ccgcgtaca attattcac gtcaaggca aatgatacc gccttttta ccatgtatc aacctatcg gacaacaat ttaaacttt agaaaggga cagacaatt gcaagggcc tggccctgc attgaagaa gaaccaccc actgagatg gtcttgcgg gtaaataac tcaccccca accgatcta ccgctcgat caggtaaaa gtcgggccc atcaatgag aacgaaagg gatcgcctt tttcgatta ttgatggat tatcgagat tgttttgca ctcacgata gatgaactc ggatgtgca aaatcggcg gaaatggaa ataaggctc tccgaggat aaacccttc acttatcgg ccgtatcga atggcgaga tcggagcag gacacagtc aaagatatt gtgcaagag cttttggac aataatatc gtcagagaa agcgagtca aattactgc tcaccggta ttattagtc aaaaagaaa aatggcgaa caaagattg tgcatcgat taccggaag ttaaatgca caaacggtt aaggacaac catccccta cctcgagtg gacgatcaa attgatcgg ctgcagggt ggcgtgtat ttcacgagt ctcgatttg cggtcgggt taccatcaa atcccgcta agtgaagag tcgaagaag tatacttca tttgttacc cccttcggt caatatgag tacaacaga gtgccattc ggactgacg aatgcgccg cgaacgttt cagcgattc atgaataag attttgaaa ccagcgcga gaaaacgcc gccgtgtat ttggatgac gtcttttta cacgcaaaa gacgtcaac gaagcgttg caaaattta caaaaagtg ttcgagatt ctcagaagc gaaggatta acacttaac ttgaaaaag tgttcgttt ctgatgacc tccgtgaca tttttgaga tttgatatc gaggacgga aaaatcaaa cccggtcaa gataagacc aaagccata gaggcatat accgctccg aaatcagta caccaaatt agacaattc atcgggttg acgggatat ttccgtcac ttcgtcaaa gattacgcg ttgatcgct aaacctttg acaaatctg acgaagaaa agtaccgcc tggaattgg ggcgacaaa gaggaagaa gcgttccgc aaattacag gcggcactt gtgacgcgc ccgacgtta gtattattt aatgccgag ctagagacc gaagttcac accgacgcc agtgcgtta gggctggcc ggaatttta ttgcaaaaa caacccgac gagcgatta catccagtg gcgtactac agccggcaa acgaccgat actgagcaa aaatatcat tcgtacgag ctcgagaca ctagccgtc gtggaaagc ttaaagaaa tttcgatct tacctgctc ggtataaaa tttaccgtc gtgaccgac tgtaatgca ttacgagcg acgagcgtt aaaaaacaa ataatcccg cgcattgcc agatggtgg ctccaactg caagaattt acatttgag gtgaagtac agacccgga aatcgtatg aaacatgtc gatgcgtta agcagaaat ccggtggaa tcgggagaa atcggcgaa gtacttcac atcgaagag gcggattgg gtcctatcg gggcaacta accgacgac aaaataaag cgcatccgc caaattttg tcgaaaccc ccgacaaat gacgaagaa cggaagatt tataaaaat tacgcccta cgcgatgga cgcgtgtat cgcattacc gtgcgggga atccaatgg gttgtccct agagggatg agacaccag gtagtgcga gccgcacac gacgatttc gggcatttt gcggtggaa aagacattg tcccgactg tgcgagcat tattggttc cctaggatg cgtaagtac gtagagcaa tacatcgct tgctgcatt gcgtgtttg tacaataag cgcgcatcg ggtagaaaa gaagggttt cttcatccc atcaagaag accgcggaa ccgctaaat gtgttccat gttgatcat ttaggacca ttccccaaa agtcgtcga ggtcacacg cacatcatc gccggaatc gacgcgttt acaaaattc gtttttctc cgtgcagtc aagtcgacc aaagtaaaa tacgtaacc gaatatttc cgtgatatt ttcgccacg tatggcaaa ccagagatt ttaatatca gatcaagga agctgtttt acttcaaaa cgcttcaaa caattttgc cgacaaaat aatattcgt cacgtgatg aacgcagtc gcgacgcca agggcgaac ggccaagtt gagcgccta aatcggacg attttagcg gccctttta tcttcgaca cttgaggag gaacggtgg gacgagcac atcaggaat gtgcagttt gcaattaat aacgttgca aataagtcc acccggaaa acaccatcc caattgtta ctaggatat gtaccgcgc aacggaact gatgtcgta cttcgcgat gaaatcacc caagtatct tccgttgta gaggattta gtagctact cgagaggaa gcactcgca aagatgtcc caggcacaa gataatcag aaaaagaat tttgacaag cgacgaaaa cgaccacgg cgctacaaa cagggtgac ctagtgttg gtggaaaag aacgtcgct tcaaacaca ggtaccagc cgaaaatta ataccccct tacagcggc ccgatggtg gtaaaaaca gtattacct aatgatcgc tacgtcgta accgacatg aaagggtca cacagaact tcgaaaaag accaattac gaacgcgta gttgctgtc gatcggatg cgaccatgg cgcgtgcca ggaggagtg tcagatgac accgacagc gaaagtggg gaagacgag attgtccca tctgacgag gacccggaa gtgacagac gatagtgat aagaagaat agtgatgtg agcgatgct gaagaagag tcacgagga tctgtcagc gagctacaa tttacagct gaagagtta aacgcacac ccagaagtt ctatcaaag tcgcaaata ccttcaatt aagaagaaa aaagacgag actgttgca aagatggtc caaaaatat aaatcgtta tatggaaaa gagactacc ggagatgta aacccaccc gttactaaa attccaggt gctaaaagt tgtggaatt ttaaaagaa aatcaaaac cctgaagtc ctagaacca attcttgct cctttacaa gctggaaca tctgcaaac acactggaa tcccgtaaa atacgaaga aacaaaaaa gttgacaaa ttgtga
- >TC032951 RA
ttggtgggc tgtattacg ggaagaacg aactccctc atcgcatat gattgtcaa aatgcgtct actaacatc actgttgtg tcactgaac gacgtatct ccgtgtcca gatcctacc cttagttac agttcttaa cccaagtcc atacaagta atccagaaa aatcaattt gaaaaaata cacgtgaaa acttgtcta atggaagta acccgtcta atcatgcac tgtggaatg tttggtctc tccagcatg gtttcacgc ggcttatac acctatctc cataagaca ggacgagat ggctgtctt ggcatccat gaacatcaa gaatatcgc ggattctat ggacatgtc ttctcaagg ttaatacct aatagtacc agtggagga acaattaca atagccgga agtgttagc atggatgga aaatgtact gggactagt tacaccgac aatggaata acttgggac gacgtggtt gttacggct tcagtcaaa ataactgtt caagaatat gaagccaac ttgaagctg gatcaggac gaaataagt ttgagagga gtcgcttgt tcattcctg acaggatac tgtatcgac gctgtcatt ggagaaact gtctggaat ccagtagcg ttaccagac tgtgataaa tattcattg atattttgc ggaaaagga cacataatt cgtcaggtg aacgaccct caagcaaca agctactta gtggtggaa cagggcgat aaagtattt gcattatca ctcatgaaa cccgacgcg gtgtgtgga atgcgcgtt tggcaaact gaacatcct aaattatta gtgacagaa accgaaccg gaactcatt tcacgaaat tggaaacaa caagtgacc accgagaac atcgattta ccagcttat atcaattcg aaattcctc tacatggaa caatcactg aaaacttca gttgaccgt gcctatgct tacaccttc catcgaaga tgtcttctt agacgagaa atcttgaag aatcgatta gtgttggca cctttatct ccaaatgta gtcagcaca ttgatgagc ggaaataat aagaggaag caaactcgt ggcgaatcg ccaattaac gcgcgaagg gttagaggc agcaccgac gggtctgat caaaccgtc tcttcgatc gaccgcctc accgaagtg atggcagat tttctcgag cggtcaaac aaccgccct accgcgttt gccacaaag gaggaggtg gtaccaccc ttcaaccca gaggaccgc gaccaaagc gccgaggcc tggtgtcgc aaggtggac gagttaagg ggtattttc cgttggacg gaagatgcc acaatttat tatgctttg gcgaaattg cggggactc gccgaggtg tggtacaaa gggttaccc tccgtaaac atgtcttgg gcggaatgg aaggaaaaa ttaaggact gcgttccca tccactcgc gatttcaat gacgatctc tcgagaatg atgcagcgg cagaaacgt cgggatgaa agctacgct cggtacttt tatgaaaag ctttcgctc ataaacgct tgccgcatc ataggaacc gacgccgtt tcatgctta atcgggggc attgacgac gtcgttgta aaaaacggg cgcgagagc aggcaatca ccaaacccc ggaatcgct tttcgcata cctcgcgac gatcagcga cacaaaagc gctcccgag accagctac agcgaccac ttcgtcgtt ctcacgagc gtcccaggt ggcaaaaac ccatcaccg aggaagaca attcgacga gaaacggcc ttcggtgcc ggtttgcta cgattgcgg aaaagaagg gcactatcg ccgacagtg ccggaagcc agcaccgga aaacagccg gaaatcgga gctgcgatg caactactg tcgtggtac cggtcacac ggagaaata ttgcaacaa gaaaaacaa agacccgaa gacgggtct acgaccttc gagctaaca gctgatcgg gcaacggca accgttcgg gcccacgtg gtcccaaat tctgtccag gaagtacct gtcctaatc ggtcgcaat ttcaccgag atgccccac attgtcatc gtaaaggac cagacctcg ttaaatata atcgcaacc gacgcagaa tcaacactg tcaaaaatc acacctgaa ccggcatta tcaaaaatc aaacttcga gtcgcgcaa aacaccatc atcccgcaa aatcacgcc ggtcatatt gcagtttcc ggagacgag tatgagggt gatgtcctc attgaagct gcgataaga cctcaagaa ggtcatgaa tactgtcta ccgcgtaca attattcac gtcaaggca aatgatacc gccttttta ccatgtatc aacctatcg gacaacaat ttaaacttt agaaaggga cagacaatt gcaagggcc tggccctgc attgaagaa gaaccaccc actgagatg gtcttgcgg gtaaataac tcaccccca accgatcta ccgctcgat caggtaaaa gtcgggccc atcaatgag aacgaaagg gatcgcctt tttcgatta ttgatggat tatcgagat tgttttgca ctcacgata gatgaactc ggatgtgca aaatcggcg gaaatggaa ataaggctc tccgaggat aaacccttc acttatcgg ccgtatcga atggcgaga tcggagcag gacacagtc aaagatatt gtgcaagag cttttggac aataatatc gtcagagaa agtgagtca aattactgc tcaccggta ttattagtc aaaaagaaa aatggcgaa caaagattg tgcatcgat taccggaag ttaaatgca caaacggtt aaggacaac catccccta cctcgggtg gacgatcaa attgatcgg ctgcagggt ggcgtgtat ttcacgagt ctcgatttg cggtcgggt taccatcaa atcccgcta agtgaagag tcgaagaag tatacttca tttgtaacc cccttcggt caatatgag tacaacaga gtgccattc ggactgacg aatgcgccg cgaacgttt cagcgattc atgaataag atcttgaaa ccagcgcga gaaaacgcc gccgtgtat ttggatgac gtcctttta cacgcaaaa gacgtcaac gaagcgtta caaaatcta caaaaagtg ttcgagatt ctcagaagc gaaggatta acacttaac ttgaaaaag tgttcgttt ctgatgacc tccgtgaca tttttggga tttgatatc gaggacgga aaaatcaaa cccggtcaa gataaaacc aaagccata gaggcatat accgctccg aaatcagta caccagatt agacaattc atcgggttg acgggatat ttccgtcac ttcgtcaaa gattacgcg ttgatcgcg aaacctttg acaaatctg acgaagaaa agtaccgcc tggaattgg ggcgacaaa gaggaagag gcgttccgc aaattacag gcggcactt gtgacgcgc ccgacgtta gtattattt aatgccgag ctagagacc ggagttcac accgacgcc agtgcgtta gggctggcc ggaatttta ttgcaaaaa caacccgac gagcgatta catccagtg gcgtactac agccggcaa acgaccgat actgagcaa aaatatcat tcgtacgag ctcgagaca ctagccgtc gtggaaagc ttaaagaaa tttcgatct tacctgctc ggtataaaa tttaccgtc gtgaccgac tgtaatgca ttacgagcg acgagcgtt aaaaaacaa ataatcccg cgcattgcc agatggtgg ctccaactg caagaattt acatttgag gtgaagtac agacccgga aatcgtatg aaacatgtc gatgcgtta agcagaaat ccggtggaa tcgggagaa atcggcgaa gtacttcac atcgaagag gcggattgg gtcctatcg gggcaacta accgacgac aaaataaag cgcatccgc caaattttg tcgaaaccc ccgacaaat gacgaagaa cggaaaatt tataaaaat tacgcccta cgcgatgga cgcgtgtat cgcattacc gtgcgggga atccaatgg gttgtccct agagggatg agacaccag gtggtgcga gccgcacac gacgatttc ggtcatttt gcggtggaa aagacattg tcccgactg tgcgagcat tattggttc cctaggatg cgtaagtac gtagagcaa tacatcgct tgctgcatt gcgtgtttg tacaataag cgcgcatcg ggtagaaaa gaagggttt cttcatccc atcaagaag accgcggaa ccgctaaat gtgttccat gttgatcat ttaggacca ttccccaaa agtcgtcga ggtcacacg cacatcatc gccggaatc gacgcgttt acaaaattc gtttttctc cgtgcagtc aagtcgacc aaagtaaaa tacgtaacc gaatatttc cgtgatatt ttcgccacg tatggcaaa ccaaagatt ttaatatca gatcaagga agctgtttt acttcaaaa cgcttcaaa caattttgc cgacaaaat aatattcgt cacgtgatg aacgcagtc gcgacgcca agggcgaac ggccaagtt gagcgccta aatcggacg attttagcg gccctttta tcttcgacc cttgaggag gaacggtgg gacgagcac atcaggaat gtgcagttc gcaattaat aacgttgca aataagtcc acccggaaa acaccatcc caattgtta ctgggatat gtaccgcgc aacggaact gatgtcgta cttcgcgat gaaatcacc caagtatct tccgttgta gaggattta gtagctact cgagaggaa gcactcgca aagatgtcc caggcacaa gataatcag aaaaagaat tttgacaag cgacgaaaa cgaccacgg cgctacaaa cagggtgac ctagtgttg gtggaaaag aacgtcgct tcaaacaca ggtaccagc cgaaaatta ataccccct tacagcggc ccgatggtg gtaaaaaca gtattacct aatgatcgc tacgtcgta accgacatg aaagggtca cacagaact tcgaaaaag accaattac gaacgcgta gttgctgtc gatcggatg cgaccatgg cgcgtgcca ggaggagtg tcagatgac accgacagt gaaagtggg gaagacgag attgtccca tctgacgag gacccggaa gtgacagac gaggttaga ggcagcacc gacgggtct gatcaaacc gtctcttcg atcgaccgc ctcaccgaa gtgatggca gattttctc gagcggtca aacaaccgc cctaccgcg tttgccaca aaggaggag gtggtacca cccttcaac ccagaggac cgcgaccaa agcgccgag gcctggtgt cgcaaggtg gacgagtta aggggtatt ttccgttgg acggaagat gccacaatt tattatgct ttggcgaaa ttgcgggga ctcgccgag gtgtggtac aaagggtta ccctccgta aacatgtct tgggcggaa tggaaggaa aaattaagg actgcgttc ccatccact cgcgatttc aatgacgat ctctcgaga atgatgcag cggcagaaa cgtcgggat gaaagctac gctcggtac ttttatgaa aagctttcg ctcataaac gcttgccgc atcatagga accgacgcc gtttcatgc ttaatcggg ggcattgac gacgtcgtt gtaaaaaac gggcgcgag agcaggcaa tcaccaaac cccggaatc gcttttcgc atacctcgc gacgatcag cgacacaaa agcgctccc gaaaccagc tacagcgac cacttcgtc gttctcacg agcacccca ggtagcaaa aacccatca ccgaggaag acaattcga cgagaaacg gtcttcggt gccggtttg ctacgattg cggaaaaga aggacacta tcgccgaca gtgccggaa gccagcacc ggaaaacag ccggaaatc ggagctgcg atgcaacta ctgtcgtgc aaaacgcgc gatccggct aatcaaaaa tattacaaa tcaatctta ataaacggt gtagaatca acggcgtat gtagattta ggtagttca tgtaccaca atacgttta gcggaggtt caaaaatta gggttggat cacgattcc aatcaaaaa accgcacta aaagctttt gggaacggg gttgttgca acgttaggg tctacgacc ttcgagcta acagctgat cgggcaacg gcaaccgtt cgggcccac gtggtccca aattctgtc caggaagta cctgtccta atcggtcgc aatttcacc gagatgccc cacattgtc atcgtaaag gaccagacc tcgttaaat ataatcgca accgacgca gaatcaaca ctgtcaaaa atcacacct gaaccggca ttatcaaaa atcaaactt cgagtcgcg caaaacacc atcatcccg caaaatcac gccggtcat attgcagtt tccggagac gagtatgag ggtgatgtc ctcattgaa gctgcgata agacctcaa gaaggtcat gaatactgt ctaccgcgt acaattatt cacgtcaag gcaaatgat accgccttt ttaccatgt atcaaccta tcggacaac aatttaaac tttagaaag ggacagaca attgcaagg gcctggccc tgcattgaa gaagaacca cccactgag atggtcttg cgggtaaat aactcaccc ccaaccgat ctaccgctc gatcaggta aaagtcggg cccatcaat gagaacgaa agggatcgc ctttttcga ttattgatg gattatcga gattgtttt gcactcacg atagatgaa ctcggatgt gcaaaatcg gcggaaatg gaaataagg ctctccgag gataaaccc ttcacttat cggccgtat cgaatggcg agatcggag caggacaca gtcaaagat attgtgcaa gagcttttg gacaataat atcgtcaga gaaagcgag tcaaattac tgctcaccg gtattatta gtcaaaaag aaaaatggc gaacaaaga ttgtgcatc gattaccgg aagttaaat gcacaaacg gttaaggac aaccatccc ctacctcga gtggacgat caaattgat cggctgcag ggtggcgtg tatttcacg agtctcgat ttgcggtcg ggttaccat caaatcccg ctaagtgaa gagtcgaag aagtatact tcatttgtt acccccttc ggtcaatat gagtacaac agagtgcca ttcggactg acgaatgcg ccgcgaacg tttcagcga ttcatgaat aagattttg aaaccagcg cgagaaaac gccgccgtg tatttggat gacgtcttt ttacacgca aaagacgtc aacgaagcg ttgcaaaat ttacaaaaa gtgttcgag attctcaga agcgaagga ttaacactt aacttgaaa aagtgttcg tttctgatg acctccgtg acatttttg agatttgat atcgaggac ggaaaaatc aaacccggt caagataag accaaagcc atagaggca tataccgct ccgaaatca gtacaccaa attagacaa ttcatcggg ttgacggga tatttccgt cacttcgtc aaagattac gcgttgatc gctaaacct ttgacaaat ctgacgaag aaaagtacc gcctggaat tggggcgac aaagaggaa gaagcgttc cgcaaatta caggcggca cttgtgacg cgcccgacg ttagtatta tttaatgcc gagctagag accgaagtt cacaccgac gccagtgcg ttagggctg gccggaatt ttattgcaa aaacaaccc gacgagcga ttacatcca gtggcgtac tacagccgg caaacgacc gatactgag caaaaatat cattcgtac gagctcgag acactagcc gtcgtggaa agcttaaag aaatttcga tcttacctg ctcggtata aaatttacc gtcgtgacc gactgtaat gcattacga gcgacgagc gttaaaaaa caaataatc ccgcgcatt gccagatgg tggctccaa ctgcaagaa tttacattt gaggtgaag tacagaccc ggaaatcgt atgaaacat gtcgatgcg ttaagcaga aatccggtg gaatcggga gaaatcggc gaagtactt cacatcgaa gaggcggat tgggtccta tcggggcaa ctaaccgac gacaaaata aagcgcatc cgccaaatt ttgtcgaaa cccccgaca aatgacgaa gaacggaag atttataaa aattacgcc ctacgcgat ggacgcgtg tatcgcatt accgtgcgg ggaatccaa tgggttgtc cctagaggg atgagacac caggtagtg cgagccgca cacgacgat ttcgggcat tttgcggtg gaaaagaca ttgtcccga ctgtgcgag cattattgg ttccctagg atgcgtaag tacgtagag caatacatc gcttgctgc attgcgtgt ttgtacaat aagcgcgca tcgggtaga aaagaaggg tttcttcat cccatcaag aagaccgcg gaaccgcta aatgtgttc catgttgat catttagga ccattcccc aaaagtcgt cgaggtcac acgcacatc atcgccgga atcgacgcg tttacaaaa ttcgttttt ctccgtgca gtcaagtcg accaaagta aaatacgta accgaatat ttccgtgat attttcgcc acgtatggc aaaccagag attttaata tcagatcaa ggaagctgt tttacttca aaacgcttc aaacaattt tgccgacaa aataatatt cgtcacgtg atgaacgca gtcgcgacg ccaagggcg aacggccaa gttgagcgc ctaaatcgg acgatttta gcggccctt ttatcttcg acacttgag gaggaacgg tgggacgag cacatcagg aatgtgcag tttgcaatt aataacgtt gcaaataag tccacccgg aaaacacca tcccaattg ttactagga tatgtaccg cgcaacgga actgatgtc gtacttcgc gatgaaatc acccaagta tcttccgtt gtagaggat ttagtagct actcgagag gaagcactc gcaaagatg tcccaggca caagataat cagaaaaag aattttgac aagcgacga aaacgacca cggcgctac aaacagggt gacctagtg ttggtggaa aagaacgtc gcttcaaac acaggtacc agccgaaaa ttaataccc ccttacagc ggcccgatg gtggtaaaa acagtatta cctaatgat cgctacgtc gtaaccgac atgaaaggg tcacacaga acttcgaaa aagaccaat tacgaacgc gtagttgct gtcgatcgg atgcgacca tggcgcgtg ccaggagga gtgtcagat gacaccgac agcgaaagt ggggaagac gagattgtc ccatctgac gaggacccg gaagtgaca gacgatagt gataagaag aatagtgat gtgagcgat gctgaagaa gagtcacga ggatctgtc agcgagcta caatttaca gctgaagag ttaaacgca cacccagaa gttctatca aagtcgcaa ataccttca attaagaag aaaaaagac gagactgtt gcaaagatg gtccaaaaa tataaatcg ttatatgga aaagagact accggagat gtaaaccca cccgttact aaaattcca ggtgctaaa agttgtgga attttaaaa gaaaatcaa aaccctgaa gtcctagaa ccaattctt gctccttta caagctgga acatctgca aacacactg gaatcccgt aaaatacga agaaacaaa aaagttgac aaattgtga gctccagcg tttagtttt gttggagca actacaaac taccagagt acaacgaga atattttaa agaaaaatt agataattt a
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