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Gene information
General information
TC008477 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11162841..11185513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008477
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04500

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs podomer slightly shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008477 RA
      MNLGLFNRM NLKESGENK GMYSEWTSL SLMLQKNGD 
      ENQSVLEKF GLGTSKEVT LGVVRQLAS NLGISQPAE 
      PSPLVTDKE VQWCMEVLC FGLSLPLSE HEAIKDCVN 
      VYCEWLSAL LSPKVCVPQ PIVEDPNVY ARKIIAHLH 
      YLFIPRKGE GIDTINRQA VLCHRVLRT LQQIAHESK 
      LLERETWEA LLLFLLAIN DTLLSPPTV KDDVADQLC 
      ERVLSVLFE IWLIACAQC FPSPPLWKT LRECCMNWR 
      HRVALIEQW NRVNLVLTS KVLAFMYGP GFPELKIGD 
      DDLITHGMS NECIAQSWY RFLNTIGNP ISLTKPEVI 
      SQTQKFLQF AISTDNVND PCQHPCLQA LPLIFLKAI 
      KGIAGQVDA FLGVAQAVW WENMITGGS TDKSKEAAP 
      NQTSATYSP SPTPPTQRR LAKSFSVAP SSVSKGIPK 
      SSLIGLTTS RSSVVATAS APNSGPSSA SSASSLTSF 
      GVETRNPLA PGRPRCNSI LHLFGEWLF EAAFLGCDL 
      HNRQTHDQN KRPSSVLME SYSQPSSIS DIPSLTIDK 
      YEGGKAEAL GTLCRIMCA KKTGEEILP VYLARFYLA 
      VQQGLKAPN RECGETMVA ILMNSSDLF RLDLDGIRV 
      LIPVFITAL EIVLPDKDL KLNSNVSKI ELRRASIHL 
      LSSMLVLPL HFQNVVIKE LISSGFSER PVTFAQLKP 
      RLMNLVMNA LQIETDPQN THMLLGSLF LCVQDSSIY 
      EKVEQVTQP PTDTSSNLL SSASDTAST MSMASSYDR 
      STSPEHFGP VDSYDLMPI DSAHALFVR ATYLVCHRL 
      ISSWKTDLN VSLAALELL SGLARIHIK ESDAMECKR 
      AVKWLCDYI VSQCSRPPP AHSKDLHST IVAAFQCCS 
      VWLLEHPYL LRDKDCLTT VLEVAELGV SGTKSIGKP 
      GENIKMKEQ KELKPASMR VRDAAENLL TCILEQVGY 
      FPNECGPES ISSLLDELA LLQHCNSWP TNCTDQAVA 
      VERFRYFVT EGSVMLALL EEPLGNDQD PQPTVTILI 
      RGPFGRHAW TMQLRHLPR HRAGMKSHA LNTGRPVVM 
      HETPVCPQV KQKYFPDSV ERVTQCKVD QSIPPLDSV 
      IAQDEETNK LVQLIERQT TVEANLAAS QSNCVPQEC 
      TPPPVCHDF QTSRLFLSH FGLLSLGDE EKADGAPTL 
      TALDSSSVD FCKDLSVLD HLSPRTCDT VHIFYVKSQ 
      QVDANDIVN NVREQSGIS AYFFEFIHT LGWPVEVNK 
      HPGWTGHVS TSWKISPQE NSVSARNSS YDGTTHVLY 
      WADACSEIA FVVPSVIKP PEPSEQSYS ASFWYERSL 
      SETSKDKRA LSLELDKQP VPPRRSGPR SNLHPHTNT 
      KIMVVWLES FEDHLNLPI DDLLNCMHT GLERGSVTK 
      SSEVLVIYL HLMASGLLR VHLQGPPGR VGLASPLID 
      GMVVSRRAL GPLVRHTAL NMARRRRLD SDSYQPPHV 
      RRRLKIQEM VQKYKREMT KPELLTYLF SST
    CDS sequence
    • >TC008477 RA
      atgaatttg gggctgttt aatcgcatg aacttgaaa 
      gaaagcggc gagaataag gggatgtat tcagaatgg 
      acgtcttta agtctcatg cttcagaag aacggcgac 
      gaaaaccaa agtgtttta gaaaagttt ggtttaggg 
      acgagcaaa gaagtgact cttggcgta gtaagacaa 
      ttggcgagt aatttgggt attagtcaa ccagcagaa 
      cctagtcct ctagtgact gataaagag gtgcaatgg 
      tgtatggaa gtactgtgc tttgggctg tctttaccc 
      ctatcagaa catgaggca atcaaagat tgtgttaat 
      gtttactgt gagtggctg tcagcgtta ctgagcccc 
      aaagtttgc gttcctcag ccaattgtt gaagatccg 
      aatgtgtac gcgcggaag attatcgcc caccttcac 
      tatttattt attccaagg aaaggagaa ggaattgat 
      acaatcaac aggcaagcc gttttgtgt cacagggtg 
      ttgcgcact ctccagcaa atcgctcac gagtcaaaa 
      ttgctcgaa cgcgagact tgggaagca cttctttta 
      tttttactc gcaataaat gacacgtta ctatcacca 
      ccaacagta aaagacgac gtagctgac caactttgc 
      gaaagagtt ttaagtgtt ttatttgag atatggctc 
      atagcgtgc gctcaatgt ttcccttca ccacctctc 
      tggaaaact ttgagagag tgttgtatg aattggcgt 
      catcgagtc gcactaatc gagcaatgg aatcgagtc 
      aatttagtt ttaacatcc aaagttttg gcgttcatg 
      tacggaccg ggtttccct gaactaaaa atcggcgat 
      gacgatttg ataactcat ggtatgtca aatgagtgt 
      atagctcag agttggtac cgttttttg aacacaatc 
      ggaaatccg attagtttg actaaacct gaagttatt 
      agccaaact cagaagttt cttcaattt gcgataagc 
      acagacaat gtaaacgac ccctgccaa catccctgt 
      cttcaggcc ctccctttg atttttctc aaagcaatc 
      aagggaata gctggtcaa gtggacgca tttctcggt 
      gtagcacaa gctgtttgg tgggaaaat atgataaca 
      gggggcagc actgataag agtaaagaa gccgcacca 
      aatcaaact tctgctact tattcacct agtcctact 
      cctcctact caacgcaga ttggcaaaa agttttagt 
      gtggcaccg tcctctgtc tctaagggt attccgaaa 
      tcgtctctg atcggtttg acgacaagt cggagttcg 
      gttgttgcg accgcttcg gccccaaat tcgggacct 
      tcgtcagct tcgagcgct tcttcttta acgtctttt 
      ggtgttgaa acacgcaac cccttagcg ccaggaagg 
      ccccgatgt aacagtatt ttgcattta tttggcgag 
      tggttattt gaagctgct tttctcggt tgcgattta 
      cacaaccga caaacacac gaccaaaat aaaagaccc 
      agttcggtt ttgatggag agttattcg caaccgagt 
      tcaatcagt gacattcct agcttgacc atagataag 
      tatgaaggt ggcaaggct gaagctttg ggtacgctt 
      tgtcgaatt atgtgtgcg aaaaaaacc ggcgaggaa 
      atacttccc gtgtattta gctcggttt tatctcgct 
      gtacaacaa gggttgaaa gcaccaaat cgcgagtgt 
      ggcgaaacc atggttgca atattaatg aactcatca 
      gatttattc cgacttgat ttagacggg attcgagtc 
      cttatccct gtttttata acagcgctt gagattgtt 
      ttaccagac aaagatttg aaattaaat tcgaatgtt 
      tcaaaaatt gaattaaga agagcttcc atccattta 
      ttgtcatca atgttggtt ttgccgcta cattttcag 
      aacgttgtg attaaggaa ttgataagt tcaggtttc 
      tcagagcgt cctgtaact ttcgctcaa ctcaaacca 
      cgattgatg aatctcgtc atgaatgca cttcagatt 
      gagaccgac cctcaaaat actcatatg ttacttgga 
      agtctgttt ttatgtgtt caagattcg tcgatttat 
      gagaaagtc gaacaagtg acacaacca ccgactgat 
      acttcctcg aatttgcta agttcagcg tccgacact 
      gccagtacc atgagcatg gctagcagt tatgaccgt 
      tccacttca cctgaacac tttggtcct gttgattcg 
      tatgattta atgccgata gattcggca catgccttg 
      tttgttagg gcgacttat ttggtatgt catcgattg 
      atcagttcg tggaaaaca gatctgaat gtttctctg 
      gcggcactt gaactgctc tcaggactc gccagaata 
      cacatcaaa gagtcggac gcgatggag tgcaaaagg 
      gccgtaaag tggctttgc gactacatc gtttcgcaa 
      tgttctcgc cctccacca gcccactcc aaagacctg 
      cactccacc atcgtggcc gctttccaa tgttgttcc 
      gtctggctc ttggaacac ccttattta ctccgcgac 
      aaagactgt cttaccaca gtccttgaa gtggcagaa 
      ttgggcgtt tctggcact aaatcaatc ggaaaaccc 
      ggcgaaaac ataaaaatg aaagaacag aaagaactc 
      aaaccggct tcgatgcga gtgcgagac gctgcggaa 
      aatctcctc acttgtatt ttagaacaa gttgggtat 
      ttccccaat gaatgcggc ccagaatca atttcatca 
      cttcttgac gaacttgct ttgttgcaa cattgcaac 
      tcgtggccc accaactgt acggatcaa gctgttgct 
      gttgaacgt tttaggtac tttgtgact gagggctcg 
      gttatgttg gctctactg gaagaaccg ttggggaat 
      gaccaagac ccgcaaccg acagtcacg attttaata 
      cgagggcca ttcggacgg catgcctgg accatgcaa 
      ttgaggcat ttgccgagg catagagca ggcatgaaa 
      tcgcatgcc ttaaacact ggacgtcct gttgtcatg 
      cacgaaaca cccgtttgt ccgcaagtc aaacaaaag 
      tattttccc gacagtgtt gagagagtc acgcagtgc 
      aaagttgat cagtcaatt ccgccgctt gattcggtc 
      atagcccaa gacgaggaa acaaacaaa ctggtccaa 
      ttaatcgag cgacaaacg acagttgag gcaaatcta 
      gccgcctca caatcgaat tgtgtgcca caagagtgt 
      acaccgcct cctgtttgt cacgatttt cagacttct 
      aggttgttt ttgtcgcat ttcgggtta ttgtcactc 
      ggtgatgag gaaaaagcg gatggggcc ccgactttg 
      acggctttg gatagcagt tcggttgat ttttgtaag 
      gatttgtcc gttttggat catttgagt ccgaggact 
      tgtgatacc gttcatatt ttctacgtc aagtcgcaa 
      caagttgac gcaaatgat attgtgaat aatgtgagg 
      gagcaaagt gggatttcg gcctacttt ttcgagttt 
      attcacact ttgggatgg cctgttgag gtgaacaaa 
      cacccaggg tggactggc cacgtttcg acgagttgg 
      aaaatttcc cctcaggaa aactccgtt tccgcgcgt 
      aactccagc tatgacggc accacccat gtcttgtat 
      tgggccgac gcctgctct gaaatagct tttgtggtc 
      ccctctgtc ataaaacca ccagaacca tctgaacaa 
      tcttacagc gcctcattc tggtacgaa cgaagcttg 
      tctgagaca agcaaagac aagagggcc ctaagtctc 
      gaactggac aaacaaccg gtgcctcct cgacgctcg 
      gggccccgg tcaaacctc cacccgcac actaatacg 
      aaaataatg gtggtatgg ttggagagt ttcgaggat 
      cacttaaat cttccgatt gatgattta ctgaattgt 
      atgcacacc ggtttagag cgaggctcc gtcacaaaa 
      agcagcgaa gtgttggtc atttactta catttgatg 
      gcttcgggg ctgttgagg gtacatctg caagggcct 
      ccagggcgt gtggggctc gcgtcgccc cttatcgac 
      ggaatggtg gtcagtcgg cgcgccttg ggccccctt 
      gtgaggcac acggcccta aatatggct cgtaggaga 
      aggcttgac tccgatagt tatcagccc cctcatgtc 
      agacgcagg cttaaaatc caggaaatg gttcagaaa 
      tataaacgc gaaatgact aaacctgaa ttattaact 
      tatttattt agttctact taa
    mRNA sequence
    • >TC008477 RA
      ttcggtgca tgacagctg tttttgacg tctgttcac 
      gtcattatt tttatcaac ataaacaac agttaatcc 
      acatcgagc ttatgaatt tggggctgt ttaatcgca 
      tgaacttga aagaaagcg gcgagaata aggggatgt 
      attcagaat ggacgtctt taagtctca tgcttcaga 
      agaacggcg acgaaaacc aaagtgttt tagaaaagt 
      ttggtttag ggacgagca aagaagtga ctcttggcg 
      tagtaagac aattggcga gtaatttgg gtattagtc 
      aaccagcag aacctagtc ctctagtga ctgataaag 
      aggtgcaat ggtgtatgg aagtactgt gctttgggc 
      tgtctttac ccctatcag aacatgagg caatcaaag 
      attgtgtta atgtttact gtgagtggc tgtcagcgt 
      tactgagcc ccaaagttt gcgttcctc agccaattg 
      ttgaagatc cgaatgtgt acgcgcgga agattatcg 
      cccaccttc actatttat ttattccaa ggaaaggag 
      aaggaattg atacaatca acaggcaag ccgttttgt 
      gtcacaggg tgttgcgca ctctccagc aaatcgctc 
      acgagtcaa aattgctcg aacgcgaga cttgggaag 
      cacttcttt tatttttac tcgcaataa atgacacgt 
      tactatcac caccaacag taaaagacg acgtagctg 
      accaacttt gcgaaagag ttttaagtg ttttatttg 
      agatatggc tcatagcgt gcgctcaat gtttccctt 
      caccacctc tctggaaaa ctttgagag agtgttgta 
      tgaattggc gtcatcgag tcgcactaa tcgagcaat 
      ggaatcgag tcaatttag ttttaacat ccaaagttt 
      tggcgttca tgtacggac cgggtttcc ctgaactaa 
      aaatcggcg atgacgatt tgataactc atggtatgt 
      caaatgagt gtatagctc agagttggt accgttttt 
      tgaacacaa tcggaaatc cgattagtt tgactaaac 
      ctgaagtta ttagccaaa ctcagaagt ttcttcaat 
      ttgcgataa gcacagaca atgtaaacg acccctgcc 
      aacatccct gtcttcagg ccctccctt tgatttttc 
      tcaaagcaa tcaagggaa tagctggtc aagtggacg 
      catttctcg gtgtagcac aagctgttt ggtgggaaa 
      atatgataa cagggggca gcactgata agagtaaag 
      aagccgcac caaatcaaa cttctgcta cttattcac 
      ctagtccta ctcctccta ctcaacgca gattggcaa 
      aaagtttta gtgtggcac cgtcctctg tctctaagg 
      gtattccga aatcgtctc tgatcggtt tgacgacaa 
      gtcggagtt cggttgttg cgaccgctt cggccccaa 
      attcgggac cttcgtcag cttcgagcg cttcttctt 
      taacgtctt ttggtgttg aaacacgca accccttag 
      cgccaggaa ggccccgat gtaacagta ttttgcatt 
      tatttggcg agtggttat ttgaagctg cttttctcg 
      gttgcgatt tacacaacc gacaaacac acgaccaaa 
      ataaaagac ccagttcgg ttttgatgg agagttatt 
      cgcaaccga gttcaatca gtgacattc ctagcttga 
      ccatagata agtatgaag gtggcaagg ctgaagctt 
      tgggtacgc tttgtcgaa ttatgtgtg cgaaaaaaa 
      ccggcgagg aaatacttc ccgtgtatt tagctcggt 
      tttatctcg ctgtacaac aagggttga aagcaccaa 
      atcgcgagt gtggcgaaa ccatggttg caatattaa 
      tgaactcat cagatttat tccgacttg atttagacg 
      ggattcgag tccttatcc ctgttttta taacagcgc 
      ttgagattg ttttaccag acaaagatt tgaaattaa 
      attcgaatg tttcaaaaa ttgaattaa gaagagctt 
      ccatccatt tattgtcat caatgttgg ttttgccgc 
      tacattttc agaacgttg tgattaagg aattgataa 
      gttcaggtt tctcagagc gtcctgtaa ctttcgctc 
      aactcaaac cacgattga tgaatctcg tcatgaatg 
      cacttcaga ttgagaccg accctcaaa atactcata 
      tgttacttg gaagtctgt ttttatgtg ttcaagatt 
      cgtcgattt atgagaaag tcgaacaag tgacacaac 
      caccgactg atacttcct cgaatttgc taagttcag 
      cgtccgaca ctgccagta ccatgagca tggctagca 
      gttatgacc gttccactt cacctgaac actttggtc 
      ctgttgatt cgtatgatt taatgccga tagattcgg 
      cacatgcct tgtttgtta gggcgactt atttggtat 
      gtcatcgat tgatcagtt cgtggaaaa cagatctga 
      atgtttctc tggcggcac ttgaactgc tctcaggac 
      tcgccagaa tacacatca aagagtcgg acgcgatgg 
      agtgcaaaa gggccgtaa agtggcttt gcgactaca 
      tcgtttcgc aatgttctc gccctccac cagcccact 
      ccaaagacc tgcactcca ccatcgtgg ccgctttcc 
      aatgttgtt ccgtctggc tcttggaac acccttatt 
      tactccgcg acaaagact gtcttacca cagtccttg 
      aagtggcag aattgggcg tttctggca ctaaatcaa 
      tcggaaaac ccggcgaaa acataaaaa tgaaagaac 
      agaaagaac tcaaaccgg cttcgatgc gagtgcgag 
      acgctgcgg aaaatctcc tcacttgta ttttagaac 
      aagttgggt atttcccca atgaatgcg gcccagaat 
      caatttcat cacttcttg acgaacttg ctttgttgc 
      aacattgca actcgtggc ccaccaact gtacggatc 
      aagctgttg ctgttgaac gttttaggt actttgtga 
      ctgagggct cggttatgt tggctctac tggaagaac 
      cgttgggga atgaccaag acccgcaac cgacagtca 
      cgattttaa tacgagggc cattcggac ggcatgcct 
      ggaccatgc aattgaggc atttgccga ggcatagag 
      caggcatga aatcgcatg ccttaaaca ctggacgtc 
      ctgttgtca tgcacgaaa cacccgttt gtccgcaag 
      tcaaacaaa agtattttc ccgacagtg ttgagagag 
      tcacgcagt gcaaagttg atcagtcaa ttccgccgc 
      ttgattcgg tcatagccc aagacgagg aaacaaaca 
      aactggtcc aattaatcg agcgacaaa cgacagttg 
      aggcaaatc tagccgcct cacaatcga attgtgtgc 
      cacaagagt gtacaccgc ctcctgttt gtcacgatt 
      ttcagactt ctaggttgt ttttgtcgc atttcgggt 
      tattgtcac tcggtgatg aggaaaaag cggatgggg 
      ccccgactt tgacggctt tggatagca gttcggttg 
      atttttgta aggatttgt ccgttttgg atcatttga 
      gtccgagga cttgtgata ccgttcata ttttctacg 
      tcaagtcgc aacaagttg acgcaaatg atattgtga 
      ataatgtga gggagcaaa gtgggattt cggcctact 
      ttttcgagt ttattcaca ctttgggat ggcctgttg 
      aggtgaaca aacacccag ggtggactg gccacgttt 
      cgacgagtt ggaaaattt cccctcagg aaaactccg 
      tttccgcgc gtaactcca gctatgacg gcaccaccc 
      atgtcttgt attgggccg acgcctgct ctgaaatag 
      cttttgtgg tcccctctg tcataaaac caccagaac 
      catctgaac aatcttaca gcgcctcat tctggtacg 
      aacgaagct tgtctgaga caagcaaag acaagaggg 
      ccctaagtc tcgaactgg acaaacaac cggtgcctc 
      ctcgacgct cggggcccc ggtcaaacc tccacccgc 
      acactaata cgaaaataa tggtggtat ggttggaga 
      gtttcgagg atcacttaa atcttccga ttgatgatt 
      tactgaatt gtatgcaca ccggtttag agcgaggct 
      ccgtcacaa aaagcagcg aagtgttgg tcatttact 
      tacatttga tggcttcgg ggctgttga gggtacatc 
      tgcaagggc ctccagggc gtgtggggc tcgcgtcgc 
      cccttatcg acggaatgg tggtcagtc ggcgcgcct 
      tgggccccc ttgtgaggc acacggccc taaatatgg 
      ctcgtagga gaaggcttg actccgata gttatcagc 
      cccctcatg tcagacgca ggcttaaaa tccaggaaa 
      tggttcaga aatataaac gcgaaatga ctaaacctg 
      aattattaa cttatttat ttagttcta cttaaataa 
      aattaaatt gaattgatt atttttcgg cgtctcatg 
      ttttgtaac cattcctcg agttgttgt tggggctct 
      ttggggggc tggtagtag tgtttaggg ggcttgccc 
      tcttcaaga cgaacgaag taatggcag tgcatgtat 
      ttaattaat ccagctctg cctctcgca tgtcttctt 
      acccctttg ataacttgg cctttaccc acaaatgat 
      tcggctgga aatgattaa tttacatag aaattgtcg 
      tttggggag aggtaaacc cctgatgga tttatgtgt 
      acactttga atttcatta aaagttaaa aaaatat
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