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Gene information
General information
TC008471 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11107876..11112099
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008471
    iBeetle screen iB_04499

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 2
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 2

    vitellogenic egg chamber deposition degenerated - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008471 RA
      MNKASLYSK YSGLQKNDA SFVIDNYLR LIKWKPNAN 
      ILDIGSGDG NVIFELLLP KIPKHFAKF VGTDISEEM 
      VLFAKNQCN DPKIDFLQM DISATIPPE FHEYFDHIF 
      SFYCLHWVV EQRQAMKNI FDMLKPGGE MLLTFLASN 
      PIYDIYERM AKSNKWGPY MNNLKKYIS PYHHSEDPE 
      TELENLLKK EGFITHLCR VENRSYTFP SFSVLSKSV 
      SAVNPFIKK LPENEIDTY IEDYLKEVR KLKTITIET 
      CNNNDNEEK IHVPYKLFV TFASKPV
    CDS sequence
    • >TC008471 RA
      atgaacaaa gcctcactg tactcaaaa tacagcggt 
      ttgcaaaaa aacgatgcg tcttttgta atcgacaat 
      tacttgaga ctcatcaag tggaagccc aacgcgaat 
      attttagac atcggctcg ggtgacggt aatgtaata 
      ttcgagctt ttactcccg aaaatcccc aaacatttc 
      gccaaattc gtcggaacg gacatctcc gaagaaatg 
      gtccttttt gcgaaaaat cagtgcaac gatccgaaa 
      atcgatttc ctacaaatg gatatttcg gcaacaatt 
      ccgcccgaa tttcacgaa tacttcgac catattttt 
      tcgttttat tgcttgcat tgggtggtg gaacagagg 
      caagccatg aagaacata ttcgacatg ctgaaaccg 
      gggggcgaa atgctcctg actttcctt gccagcaac 
      ccgatttat gacatttac gaacgaatg gctaaatcc 
      aacaaatgg gggccatac atgaacaat ttaaaaaaa 
      tacatctcg ccctatcac cattcggag gatcctgaa 
      accgagctg gagaatttg ctgaaaaag gaagggttt 
      attacgcat ttgtgtcga gtggagaat cgctcgtac 
      acttttccc agtttttca gttttgtca aaatcggtt 
      tcagcggtg aatccgttc attaaaaaa ctgcctgaa 
      aacgaaatt gacacctac attgaggat tacctcaaa 
      gaggtcagg aaactgaag acgatcaca atcgaaacg 
      tgtaacaat aacgacaat gaagagaaa atacacgtg 
      ccgtacaaa ctcttcgtt acatttgcc tcgaaaccg 
      gtctga
    mRNA sequence
    • >TC008471 RA
      ttagttgac accaaacac ttggtctat tttctttaa 
      aaaatgaac aaagcctca ctgtactca aaatacagc 
      ggtttgcaa aaaaacgat gcgtctttt gtaatcgac 
      aattacttg agactcatc aagtggaag cccaacgcg 
      aatatttta gacatcggc tcgggtgac ggtaatgta 
      atattcgag cttttactc ccgaaaatc cccaaacat 
      ttcgccaaa ttcgtcgga acggacatc tccgaagaa 
      atggtcctt tttgcgaaa aatcagtgc aacgatccg 
      aaaatcgat ttcctacaa atggatatt tcggcaaca 
      attccgccc gaatttcac gaatacttc gaccatatt 
      ttttcgttt tattgcttg cattgggtg gtggaacag 
      aggcaagcc atgaagaac atattcgac atgctgaaa 
      ccggggggc gaaatgctc ctgactttc cttgccagc 
      aacccgatt tatgacatt tacgaacga atggctaaa 
      tccaacaaa tgggggcca tacatgaac aatttaaaa 
      aaatacatc tcgccctat caccattcg gaggatcct 
      gaaaccgag ctggagaat ttgctgaaa aaggaaggg 
      tttattacg catttgtgt cgagtggag aatcgctcg 
      tacactttt cccagtttt tcagttttg tcaaaatcg 
      gtttcagcg gtgaatccg ttcattaaa aaactgcct 
      gaaaacgaa attgacacc tacattgag gattacctc 
      aaagaggtc aggaaactg aagacgatc acaatcgaa 
      acgtgtaac aataacgac aatgaagag aaaatacac 
      gtgccgtac aaactcttc gttacattt gcctcgaaa 
      ccggtctga tttcagaaa gaatagttg caggaagac 
      gagtacaaa ttattatat ttttaagaa ttatttttt 
      tatttatta ttcttagtt tcaacgtac tgaacctca 
      ataagtaca aattatctt tagatatcg tagctttat 
      agttatata tttactcta aggtaattg gtttatttt 
      ttatatatt tttttattt acgtatatt ctataaaca 
      gagtagggc ccggtaaat cgcttaaat tacatcggt 
      aacaaaaaa tttggttgt attttttta cccatgtat 
      ttcagagat ttgccaaat tattaggaa tggtttacg 
      gaatgatat tttctaaaa tatttttat ataaaaaat 
      ttaaataaa caaatgttt agata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY