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Gene information
General information
TC008467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11087876..11096374
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008467
      iBeetle screen iB_04498

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008467 RA
        MRISKGTII ILVSMLLIS IFLANVVNL LSTDGTKVN 
        LSMKDEYLK VADSSDHLI WFLQISDIH ISVFQDPFR 
        VTDLKDFCF RTVDVIKPP VVLASGDLT DAKTADHIG 
        SQQLEKEWQ YYRNVLNEC RVREKTVWL DIRGNHDNF 
        NVAGADSKQ NYYLNYSIQ GRQHSRSYI YQLSKGSDL 
        YSFIGIDAC LDPGPRRPF NFIGKLDQT EIDHINKLI 
        ERTKISGSN YTIWFGHFP TSCILSTGS EGVRNLIRK 
        DPKGLVYVC GHLHKLGGL VPKMYTLQK SGFLELELG 
        DWKDNRVFR LLAIDHGML SFVDVAHGE WPVGLITNP 
        KHAQFINSA KENLDNIRK STHIRVLAF SLGEITSVK 
        IRINDGKWM DLYRVEGPL YVLKWNPKK YLSGLHTME 
        LLIKDSHGR EKFYSQPFA LDDTRLSFS LLSRIALMT 
        DASTIFRLM FVVTLITCI LPLIVFRLL HRMAEDKKL 
        TPPRLRGSF CRMWLRKFW VLSTVDRIF WPIFLYPLY 
        LSFGPWSVG YLVEDHVGV IFAWGIFVN GSYLPGSFT 
        YAYGFIQLV TFQIPLTFI LAHGVDHSI QYKVINAQK 
        KKTICGHIG LHLPFLLIF GIQVQMAYL FWLAYGTLA 
        FILGPLRTW SLFLAAVLW YNAIYLPER CTRKAANLL 
        QIVPARDHS DSNVLDIKQ
      CDS sequence
      • >TC008467 RA
        atgaggata tccaagggc acaatcatt attttagtc 
        tcgatgcta ctcatctca atttttctt gcaaatgtt 
        gtaaatttg ttaagcact gatgggacg aaagttaac 
        ttatcaatg aaggatgaa tatctgaaa gtggcggac 
        agtagcgat cacctaatt tggttttta caaatttct 
        gacatacac attagtgtt tttcaagac ccgtttcgc 
        gtaacggat ttgaaggat ttttgcttt cgaactgtt 
        gacgtaatt aagcctcct gtggtcttg gcttctggt 
        gatttaacc gacgccaaa acagccgac cacattggc 
        tcgcaacaa ttggaaaaa gagtggcaa tattacaga 
        aatgtactc aacgaatgc cgagtcaga gaaaaaact 
        gtttggctt gatattaga ggaaatcac gataatttt 
        aacgtagca ggagctgac tctaaacaa aactactat 
        ttaaattac tcaattcaa gggcgccag cactctcgc 
        tcctacatt taccaactt tcaaagggc tccgattta 
        tactccttt attgggatc gacgcctgc ttggaccca 
        ggccctaga cgccctttc aacttcatc ggaaaactc 
        gaccaaacc gaaatcgac cacatcaac aaactgatc 
        gaaagaacg aaaatttcg ggcagtaat tacacgatt 
        tggtttggt cattttccg acttcttgc attttgagt 
        acagggagc gagggtgtt cgaaattta atccggaaa 
        gacccgaaa ggtttggtt tatgtttgt ggacatttg 
        cacaaactt gggggtctg gtgcctaaa atgtacact 
        ttgcaaaag tcgggcttt ttggagttg gaacttggg 
        gactggaaa gacaacaga gtgtttcgt ttgttggct 
        attgatcac ggaatgttg tctttcgtg gacgtcgcg 
        catggagag tggcctgtg gggcttata actaacccc 
        aaacatgca cagtttatc aactcggcg aaagagaat 
        cttgacaat attagaaag tccactcat atcagagtg 
        ttagcgttt tcgttagga gagataacg tctgtaaaa 
        atacgtata aacgatggt aaatggatg gacctgtac 
        cgtgtagag ggtcctcta tacgtactg aagtggaac 
        cccaagaaa tacctttca gggctccac accatggaa 
        ttattaatt aaagactct cacggaagg gaaaagttt 
        tactcacaa cccttcgca ctggacgat actcgtcta 
        tcatttagt cttttgtct cgaatagct ttaatgact 
        gatgctagc accatcttt cgattgatg ttcgttgtg 
        acgttaatt acgtgcata ttaccctta atcgtattt 
        cgacttctc cacagaatg gcggaagac aaaaaatta 
        actcctccg cgcctcaga ggctctttt tgtcggatg 
        tggttgcgt aaattctgg gtgctctca accgttgat 
        cggattttt tggcctata tttttatac cctctttac 
        ctgtccttc ggaccatgg tcggtggga tacttggtg 
        gaggaccat gtgggggtg atttttgca tggggtatt 
        ttcgtcaat ggctcctac ttacccggt tctttcact 
        tacgcttat ggatttatc cagttggtt acattccaa 
        ataccttta acgtttatt ctggcccat ggagtggat 
        cacagtatc caatataaa gtgataaat gcacaaaaa 
        aagaaaaca atttgtggc cacattggg ttacatttg 
        ccctttttg ctgatattc gggattcaa gtacagatg 
        gcgtacttg ttctggttg gcttatggg actttggcc 
        tttattttg gggcctttg aggacgtgg tctttgttt 
        ttggctgca gttctatgg tacaatgcc atctatcta 
        ccagagagg tgtacaagg aaagctgca aacttgttg 
        caaattgta ccagcgaga gatcacagc gacagcaat 
        gtcctcgat atcaagcag taa
      mRNA sequence
      • >TC008467 RA
        aaaagaaat gacacttga catttgtca aagtggttg 
        tgatgattg gaaattttt aaattcatt gtgcaataa 
        attcttgaa taagccggt gtattgtca cacaaccaa 
        gcgatgaat agtcaagaa cagacggtg cggttttac 
        tacaacaat caaaaatca tgaggatat ccaagggca 
        caatcatta ttttagtct cgatgctac tcatctcaa 
        tttttcttg caaatgttg taaatttgt taagcactg 
        atgggacga aagttaact tatcaatga aggatgaat 
        atctgaaag tggcggaca gtagcgatc acctaattt 
        ggtttttac aaatttctg acatacaca ttagtgttt 
        ttcaagacc cgtttcgcg taacggatt tgaaggatt 
        tttgctttc gaactgttg acgtaatta agcctcctg 
        tggtcttgg cttctggtg atttaaccg acgccaaaa 
        cagccgacc acattggct cgcaacaat tggaaaaag 
        agtggcaat attacagaa atgtactca acgaatgcc 
        gagtcagag aaaaaactg tttggcttg atattagag 
        gaaatcacg ataatttta acgtagcag gagctgact 
        ctaaacaaa actactatt taaattact caattcaag 
        ggcgccagc actctcgct cctacattt accaacttt 
        caaagggct ccgatttat actccttta ttgggatcg 
        acgcctgct tggacccag gccctagac gccctttca 
        acttcatcg gaaaactcg accaaaccg aaatcgacc 
        acatcaaca aactgatcg aaagaacga aaatttcgg 
        gcagtaatt acacgattt ggtttggtc attttccga 
        cttcttgca ttttgagta cagggagcg agggtgttc 
        gaaatttaa tccggaaag acccgaaag gtttggttt 
        atgtttgtg gacatttgc acaaacttg ggggtctgg 
        tgcctaaaa tgtacactt tgcaaaagt cgggctttt 
        tggagttgg aacttgggg actggaaag acaacagag 
        tgtttcgtt tgttggcta ttgatcacg gaatgttgt 
        ctttcgtgg acgtcgcgc atggagagt ggcctgtgg 
        ggcttataa ctaacccca aacatgcac agtttatca 
        actcggcga aagagaatc ttgacaata ttagaaagt 
        ccactcata tcagagtgt tagcgtttt cgttaggag 
        agataacgt ctgtaaaaa tacgtataa acgatggta 
        aatggatgg acctgtacc gtgtagagg gtcctctat 
        acgtactga agtggaacc ccaagaaat acctttcag 
        ggctccaca ccatggaat tattaatta aagactctc 
        acggaaggg aaaagtttt actcacaac ccttcgcac 
        tggacgata ctcgtctat catttagtc ttttgtctc 
        gaatagctt taatgactg atgctagca ccatctttc 
        gattgatgt tcgttgtga cgttaatta cgtgcatat 
        tacccttaa tcgtatttc gacttctcc acagaatgg 
        cggaagaca aaaaattaa ctcctccgc gcctcagag 
        gctcttttt gtcggatgt ggttgcgta aattctggg 
        tgctctcaa ccgttgatc ggatttttt ggcctatat 
        ttttatacc ctctttacc tgtccttcg gaccatggt 
        cggtgggat acttggtgg aggaccatg tgggggtga 
        tttttgcat ggggtattt tcgtcaatg gctcctact 
        tacccggtt ctttcactt acgcttatg gatttatcc 
        agttggtta cattccaaa tacctttaa cgtttattc 
        tggcccatg gagtggatc acagtatcc aatataaag 
        tgataaatg cacaaaaaa agaaaacaa tttgtggcc 
        acattgggt tacatttgc cctttttgc tgatattcg 
        ggattcaag tacagatgg cgtacttgt tctggttgg 
        cttatggga ctttggcct ttattttgg ggcctttga 
        ggacgtggt ctttgtttt tggctgcag ttctatggt 
        acaatgcca tctatctac cagagaggt gtacaagga 
        aagctgcaa acttgttgc aaattgtac cagcgagag 
        atcacagcg acagcaatg tcctcgata tcaagcagt 
        aattcgatg tgtcttttc ctaaatgta ctgatattc 
        aatgttgaa caaatcatt tcctcagtt taggttacg 
        acagggcca tacaagaaa ttataccaa atatattta 
        tcgatctat ttgtgcttt caataaatt actgtgaga 
        ctttgcaat attattgtt agacgttaa tacggctca 
        tgtgatagt ttttgttag tattataat tgtagttaa 
        tttttttct tccattccc acattctta acaaggtga 
        aacctattt ttatatatc tttatccct ctagtttgt 
        gttatcgat caagaattc taaatgcaa tattgtact 
        ttcgacgtc aatcaataa ataaatatt gcagcagtt 
        accattgcg ttttaagca ccttttcca ataaacaat 
        ctggctatt g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY