| General information | |||
| TC008467 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 11087876..11096374 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008467 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04498 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC008467 RA
MRISKGTII ILVSMLLIS IFLANVVNL LSTDGTKVN LSMKDEYLK VADSSDHLI WFLQISDIH ISVFQDPFR VTDLKDFCF RTVDVIKPP VVLASGDLT DAKTADHIG SQQLEKEWQ YYRNVLNEC RVREKTVWL DIRGNHDNF NVAGADSKQ NYYLNYSIQ GRQHSRSYI YQLSKGSDL YSFIGIDAC LDPGPRRPF NFIGKLDQT EIDHINKLI ERTKISGSN YTIWFGHFP TSCILSTGS EGVRNLIRK DPKGLVYVC GHLHKLGGL VPKMYTLQK SGFLELELG DWKDNRVFR LLAIDHGML SFVDVAHGE WPVGLITNP KHAQFINSA KENLDNIRK STHIRVLAF SLGEITSVK IRINDGKWM DLYRVEGPL YVLKWNPKK YLSGLHTME LLIKDSHGR EKFYSQPFA LDDTRLSFS LLSRIALMT DASTIFRLM FVVTLITCI LPLIVFRLL HRMAEDKKL TPPRLRGSF CRMWLRKFW VLSTVDRIF WPIFLYPLY LSFGPWSVG YLVEDHVGV IFAWGIFVN GSYLPGSFT YAYGFIQLV TFQIPLTFI LAHGVDHSI QYKVINAQK KKTICGHIG LHLPFLLIF GIQVQMAYL FWLAYGTLA FILGPLRTW SLFLAAVLW YNAIYLPER CTRKAANLL QIVPARDHS DSNVLDIKQ
- >TC008467 RA
atgaggata tccaagggc acaatcatt attttagtc tcgatgcta ctcatctca atttttctt gcaaatgtt gtaaatttg ttaagcact gatgggacg aaagttaac ttatcaatg aaggatgaa tatctgaaa gtggcggac agtagcgat cacctaatt tggttttta caaatttct gacatacac attagtgtt tttcaagac ccgtttcgc gtaacggat ttgaaggat ttttgcttt cgaactgtt gacgtaatt aagcctcct gtggtcttg gcttctggt gatttaacc gacgccaaa acagccgac cacattggc tcgcaacaa ttggaaaaa gagtggcaa tattacaga aatgtactc aacgaatgc cgagtcaga gaaaaaact gtttggctt gatattaga ggaaatcac gataatttt aacgtagca ggagctgac tctaaacaa aactactat ttaaattac tcaattcaa gggcgccag cactctcgc tcctacatt taccaactt tcaaagggc tccgattta tactccttt attgggatc gacgcctgc ttggaccca ggccctaga cgccctttc aacttcatc ggaaaactc gaccaaacc gaaatcgac cacatcaac aaactgatc gaaagaacg aaaatttcg ggcagtaat tacacgatt tggtttggt cattttccg acttcttgc attttgagt acagggagc gagggtgtt cgaaattta atccggaaa gacccgaaa ggtttggtt tatgtttgt ggacatttg cacaaactt gggggtctg gtgcctaaa atgtacact ttgcaaaag tcgggcttt ttggagttg gaacttggg gactggaaa gacaacaga gtgtttcgt ttgttggct attgatcac ggaatgttg tctttcgtg gacgtcgcg catggagag tggcctgtg gggcttata actaacccc aaacatgca cagtttatc aactcggcg aaagagaat cttgacaat attagaaag tccactcat atcagagtg ttagcgttt tcgttagga gagataacg tctgtaaaa atacgtata aacgatggt aaatggatg gacctgtac cgtgtagag ggtcctcta tacgtactg aagtggaac cccaagaaa tacctttca gggctccac accatggaa ttattaatt aaagactct cacggaagg gaaaagttt tactcacaa cccttcgca ctggacgat actcgtcta tcatttagt cttttgtct cgaatagct ttaatgact gatgctagc accatcttt cgattgatg ttcgttgtg acgttaatt acgtgcata ttaccctta atcgtattt cgacttctc cacagaatg gcggaagac aaaaaatta actcctccg cgcctcaga ggctctttt tgtcggatg tggttgcgt aaattctgg gtgctctca accgttgat cggattttt tggcctata tttttatac cctctttac ctgtccttc ggaccatgg tcggtggga tacttggtg gaggaccat gtgggggtg atttttgca tggggtatt ttcgtcaat ggctcctac ttacccggt tctttcact tacgcttat ggatttatc cagttggtt acattccaa ataccttta acgtttatt ctggcccat ggagtggat cacagtatc caatataaa gtgataaat gcacaaaaa aagaaaaca atttgtggc cacattggg ttacatttg ccctttttg ctgatattc gggattcaa gtacagatg gcgtacttg ttctggttg gcttatggg actttggcc tttattttg gggcctttg aggacgtgg tctttgttt ttggctgca gttctatgg tacaatgcc atctatcta ccagagagg tgtacaagg aaagctgca aacttgttg caaattgta ccagcgaga gatcacagc gacagcaat gtcctcgat atcaagcag taa
- >TC008467 RA
aaaagaaat gacacttga catttgtca aagtggttg tgatgattg gaaattttt aaattcatt gtgcaataa attcttgaa taagccggt gtattgtca cacaaccaa gcgatgaat agtcaagaa cagacggtg cggttttac tacaacaat caaaaatca tgaggatat ccaagggca caatcatta ttttagtct cgatgctac tcatctcaa tttttcttg caaatgttg taaatttgt taagcactg atgggacga aagttaact tatcaatga aggatgaat atctgaaag tggcggaca gtagcgatc acctaattt ggtttttac aaatttctg acatacaca ttagtgttt ttcaagacc cgtttcgcg taacggatt tgaaggatt tttgctttc gaactgttg acgtaatta agcctcctg tggtcttgg cttctggtg atttaaccg acgccaaaa cagccgacc acattggct cgcaacaat tggaaaaag agtggcaat attacagaa atgtactca acgaatgcc gagtcagag aaaaaactg tttggcttg atattagag gaaatcacg ataatttta acgtagcag gagctgact ctaaacaaa actactatt taaattact caattcaag ggcgccagc actctcgct cctacattt accaacttt caaagggct ccgatttat actccttta ttgggatcg acgcctgct tggacccag gccctagac gccctttca acttcatcg gaaaactcg accaaaccg aaatcgacc acatcaaca aactgatcg aaagaacga aaatttcgg gcagtaatt acacgattt ggtttggtc attttccga cttcttgca ttttgagta cagggagcg agggtgttc gaaatttaa tccggaaag acccgaaag gtttggttt atgtttgtg gacatttgc acaaacttg ggggtctgg tgcctaaaa tgtacactt tgcaaaagt cgggctttt tggagttgg aacttgggg actggaaag acaacagag tgtttcgtt tgttggcta ttgatcacg gaatgttgt ctttcgtgg acgtcgcgc atggagagt ggcctgtgg ggcttataa ctaacccca aacatgcac agtttatca actcggcga aagagaatc ttgacaata ttagaaagt ccactcata tcagagtgt tagcgtttt cgttaggag agataacgt ctgtaaaaa tacgtataa acgatggta aatggatgg acctgtacc gtgtagagg gtcctctat acgtactga agtggaacc ccaagaaat acctttcag ggctccaca ccatggaat tattaatta aagactctc acggaaggg aaaagtttt actcacaac ccttcgcac tggacgata ctcgtctat catttagtc ttttgtctc gaatagctt taatgactg atgctagca ccatctttc gattgatgt tcgttgtga cgttaatta cgtgcatat tacccttaa tcgtatttc gacttctcc acagaatgg cggaagaca aaaaattaa ctcctccgc gcctcagag gctcttttt gtcggatgt ggttgcgta aattctggg tgctctcaa ccgttgatc ggatttttt ggcctatat ttttatacc ctctttacc tgtccttcg gaccatggt cggtgggat acttggtgg aggaccatg tgggggtga tttttgcat ggggtattt tcgtcaatg gctcctact tacccggtt ctttcactt acgcttatg gatttatcc agttggtta cattccaaa tacctttaa cgtttattc tggcccatg gagtggatc acagtatcc aatataaag tgataaatg cacaaaaaa agaaaacaa tttgtggcc acattgggt tacatttgc cctttttgc tgatattcg ggattcaag tacagatgg cgtacttgt tctggttgg cttatggga ctttggcct ttattttgg ggcctttga ggacgtggt ctttgtttt tggctgcag ttctatggt acaatgcca tctatctac cagagaggt gtacaagga aagctgcaa acttgttgc aaattgtac cagcgagag atcacagcg acagcaatg tcctcgata tcaagcagt aattcgatg tgtcttttc ctaaatgta ctgatattc aatgttgaa caaatcatt tcctcagtt taggttacg acagggcca tacaagaaa ttataccaa atatattta tcgatctat ttgtgcttt caataaatt actgtgaga ctttgcaat attattgtt agacgttaa tacggctca tgtgatagt ttttgttag tattataat tgtagttaa tttttttct tccattccc acattctta acaaggtga aacctattt ttatatatc tttatccct ctagtttgt gttatcgat caagaattc taaatgcaa tattgtact ttcgacgtc aatcaataa ataaatatt gcagcagtt accattgcg ttttaagca ccttttcca ataaacaat ctggctatt g
