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Gene information
General information
TC008467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11087876..11096374
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC008467
      iBeetle screen iB_04498

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008467 RA
        MRISKGTII ILVSMLLIS IFLANVVNL LSTDGTKVN 
LSMKDEYLK VADSSDHLI WFLQISDIH ISVFQDPFR 
VTDLKDFCF RTVDVIKPP VVLASGDLT DAKTADHIG 
SQQLEKEWQ YYRNVLNEC RVREKTVWL DIRGNHDNF 
NVAGADSKQ NYYLNYSIQ GRQHSRSYI YQLSKGSDL 
YSFIGIDAC LDPGPRRPF NFIGKLDQT EIDHINKLI 
ERTKISGSN YTIWFGHFP TSCILSTGS EGVRNLIRK 
DPKGLVYVC GHLHKLGGL VPKMYTLQK SGFLELELG 
DWKDNRVFR LLAIDHGML SFVDVAHGE WPVGLITNP 
KHAQFINSA KENLDNIRK STHIRVLAF SLGEITSVK 
IRINDGKWM DLYRVEGPL YVLKWNPKK YLSGLHTME 
LLIKDSHGR EKFYSQPFA LDDTRLSFS LLSRIALMT 
DASTIFRLM FVVTLITCI LPLIVFRLL HRMAEDKKL 
TPPRLRGSF CRMWLRKFW VLSTVDRIF WPIFLYPLY 
LSFGPWSVG YLVEDHVGV IFAWGIFVN GSYLPGSFT 
YAYGFIQLV TFQIPLTFI LAHGVDHSI QYKVINAQK 
KKTICGHIG LHLPFLLIF GIQVQMAYL FWLAYGTLA 
FILGPLRTW SLFLAAVLW YNAIYLPER CTRKAANLL 
QIVPARDHS DSNVLDIKQ
      CDS sequence
      • >TC008467 RA
        atgaggata tccaagggc acaatcatt attttagtc 
tcgatgcta ctcatctca atttttctt gcaaatgtt 
gtaaatttg ttaagcact gatgggacg aaagttaac 
ttatcaatg aaggatgaa tatctgaaa gtggcggac 
agtagcgat cacctaatt tggttttta caaatttct 
gacatacac attagtgtt tttcaagac ccgtttcgc 
gtaacggat ttgaaggat ttttgcttt cgaactgtt 
gacgtaatt aagcctcct gtggtcttg gcttctggt 
gatttaacc gacgccaaa acagccgac cacattggc 
tcgcaacaa ttggaaaaa gagtggcaa tattacaga 
aatgtactc aacgaatgc cgagtcaga gaaaaaact 
gtttggctt gatattaga ggaaatcac gataatttt 
aacgtagca ggagctgac tctaaacaa aactactat 
ttaaattac tcaattcaa gggcgccag cactctcgc 
tcctacatt taccaactt tcaaagggc tccgattta 
tactccttt attgggatc gacgcctgc ttggaccca 
ggccctaga cgccctttc aacttcatc ggaaaactc 
gaccaaacc gaaatcgac cacatcaac aaactgatc 
gaaagaacg aaaatttcg ggcagtaat tacacgatt 
tggtttggt cattttccg acttcttgc attttgagt 
acagggagc gagggtgtt cgaaattta atccggaaa 
gacccgaaa ggtttggtt tatgtttgt ggacatttg 
cacaaactt gggggtctg gtgcctaaa atgtacact 
ttgcaaaag tcgggcttt ttggagttg gaacttggg 
gactggaaa gacaacaga gtgtttcgt ttgttggct 
attgatcac ggaatgttg tctttcgtg gacgtcgcg 
catggagag tggcctgtg gggcttata actaacccc 
aaacatgca cagtttatc aactcggcg aaagagaat 
cttgacaat attagaaag tccactcat atcagagtg 
ttagcgttt tcgttagga gagataacg tctgtaaaa 
atacgtata aacgatggt aaatggatg gacctgtac 
cgtgtagag ggtcctcta tacgtactg aagtggaac 
cccaagaaa tacctttca gggctccac accatggaa 
ttattaatt aaagactct cacggaagg gaaaagttt 
tactcacaa cccttcgca ctggacgat actcgtcta 
tcatttagt cttttgtct cgaatagct ttaatgact 
gatgctagc accatcttt cgattgatg ttcgttgtg 
acgttaatt acgtgcata ttaccctta atcgtattt 
cgacttctc cacagaatg gcggaagac aaaaaatta 
actcctccg cgcctcaga ggctctttt tgtcggatg 
tggttgcgt aaattctgg gtgctctca accgttgat 
cggattttt tggcctata tttttatac cctctttac 
ctgtccttc ggaccatgg tcggtggga tacttggtg 
gaggaccat gtgggggtg atttttgca tggggtatt 
ttcgtcaat ggctcctac ttacccggt tctttcact 
tacgcttat ggatttatc cagttggtt acattccaa 
ataccttta acgtttatt ctggcccat ggagtggat 
cacagtatc caatataaa gtgataaat gcacaaaaa 
aagaaaaca atttgtggc cacattggg ttacatttg 
ccctttttg ctgatattc gggattcaa gtacagatg 
gcgtacttg ttctggttg gcttatggg actttggcc 
tttattttg gggcctttg aggacgtgg tctttgttt 
ttggctgca gttctatgg tacaatgcc atctatcta 
ccagagagg tgtacaagg aaagctgca aacttgttg 
caaattgta ccagcgaga gatcacagc gacagcaat 
gtcctcgat atcaagcag taa
      mRNA sequence
      • >TC008467 RA
        aaaagaaat gacacttga catttgtca aagtggttg 
tgatgattg gaaattttt aaattcatt gtgcaataa 
attcttgaa taagccggt gtattgtca cacaaccaa 
gcgatgaat agtcaagaa cagacggtg cggttttac 
tacaacaat caaaaatca tgaggatat ccaagggca 
caatcatta ttttagtct cgatgctac tcatctcaa 
tttttcttg caaatgttg taaatttgt taagcactg 
atgggacga aagttaact tatcaatga aggatgaat 
atctgaaag tggcggaca gtagcgatc acctaattt 
ggtttttac aaatttctg acatacaca ttagtgttt 
ttcaagacc cgtttcgcg taacggatt tgaaggatt 
tttgctttc gaactgttg acgtaatta agcctcctg 
tggtcttgg cttctggtg atttaaccg acgccaaaa 
cagccgacc acattggct cgcaacaat tggaaaaag 
agtggcaat attacagaa atgtactca acgaatgcc 
gagtcagag aaaaaactg tttggcttg atattagag 
gaaatcacg ataatttta acgtagcag gagctgact 
ctaaacaaa actactatt taaattact caattcaag 
ggcgccagc actctcgct cctacattt accaacttt 
caaagggct ccgatttat actccttta ttgggatcg 
acgcctgct tggacccag gccctagac gccctttca 
acttcatcg gaaaactcg accaaaccg aaatcgacc 
acatcaaca aactgatcg aaagaacga aaatttcgg 
gcagtaatt acacgattt ggtttggtc attttccga 
cttcttgca ttttgagta cagggagcg agggtgttc 
gaaatttaa tccggaaag acccgaaag gtttggttt 
atgtttgtg gacatttgc acaaacttg ggggtctgg 
tgcctaaaa tgtacactt tgcaaaagt cgggctttt 
tggagttgg aacttgggg actggaaag acaacagag 
tgtttcgtt tgttggcta ttgatcacg gaatgttgt 
ctttcgtgg acgtcgcgc atggagagt ggcctgtgg 
ggcttataa ctaacccca aacatgcac agtttatca 
actcggcga aagagaatc ttgacaata ttagaaagt 
ccactcata tcagagtgt tagcgtttt cgttaggag 
agataacgt ctgtaaaaa tacgtataa acgatggta 
aatggatgg acctgtacc gtgtagagg gtcctctat 
acgtactga agtggaacc ccaagaaat acctttcag 
ggctccaca ccatggaat tattaatta aagactctc 
acggaaggg aaaagtttt actcacaac ccttcgcac 
tggacgata ctcgtctat catttagtc ttttgtctc 
gaatagctt taatgactg atgctagca ccatctttc 
gattgatgt tcgttgtga cgttaatta cgtgcatat 
tacccttaa tcgtatttc gacttctcc acagaatgg 
cggaagaca aaaaattaa ctcctccgc gcctcagag 
gctcttttt gtcggatgt ggttgcgta aattctggg 
tgctctcaa ccgttgatc ggatttttt ggcctatat 
ttttatacc ctctttacc tgtccttcg gaccatggt 
cggtgggat acttggtgg aggaccatg tgggggtga 
tttttgcat ggggtattt tcgtcaatg gctcctact 
tacccggtt ctttcactt acgcttatg gatttatcc 
agttggtta cattccaaa tacctttaa cgtttattc 
tggcccatg gagtggatc acagtatcc aatataaag 
tgataaatg cacaaaaaa agaaaacaa tttgtggcc 
acattgggt tacatttgc cctttttgc tgatattcg 
ggattcaag tacagatgg cgtacttgt tctggttgg 
cttatggga ctttggcct ttattttgg ggcctttga 
ggacgtggt ctttgtttt tggctgcag ttctatggt 
acaatgcca tctatctac cagagaggt gtacaagga 
aagctgcaa acttgttgc aaattgtac cagcgagag 
atcacagcg acagcaatg tcctcgata tcaagcagt 
aattcgatg tgtcttttc ctaaatgta ctgatattc 
aatgttgaa caaatcatt tcctcagtt taggttacg 
acagggcca tacaagaaa ttataccaa atatattta 
tcgatctat ttgtgcttt caataaatt actgtgaga 
ctttgcaat attattgtt agacgttaa tacggctca 
tgtgatagt ttttgttag tattataat tgtagttaa 
tttttttct tccattccc acattctta acaaggtga 
aacctattt ttatatatc tttatccct ctagtttgt 
gttatcgat caagaattc taaatgcaa tattgtact 
ttcgacgtc aatcaataa ataaatatt gcagcagtt 
accattgcg ttttaagca ccttttcca ataaacaat 
ctggctatt g
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