Status
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Gene information
General information
TC008466 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11055336..11061760
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008466
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04497

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008466 RA
      MRLNMLIYL IVACCWGKS LSIPQQILN EFKSTLLPL 
      FGLKEQPKI EGKVQVPEA LKKIYNIQN NFEYDTASL 
      PLPGLYTKS ANTIRSFTH VESPIDEKF VHPHRFRLK 
      FNISSIPRH EKLTAAEIK LTRETAKNT SHPFQRVLV 
      HDILQPGVK GLHGPITRV IDSKVVDSR KNTTVSIDV 
      FPAVARWMQ DPKTNHGIL IVVYSIGAK KSPPEKHLR 
      LRRDTAPPQ WYQHQPLLF TYTDDGKNQ QRTGTELTK 
      MRPKRQSSR RHRKNLKDP CRRRQMYVD FGSVGWNDW 
      IVAPLGYDA YYCGGECEY PIPDHMNTT NHAIVQSLV 
      NSMKPKEVP GPCCVPTQL GQMSMLYLG SDGSVILKN 
      YKEMVVVGC GCR
    CDS sequence
    • >TC008466 RA
      atgcgtttg aacatgctg atatacctc atagtggca 
      tgttgttgg ggtaaatct ctctcgata cctcaacaa 
      atactcaat gaattcaaa tcgacactg ttgcccctt 
      ttcggactc aaagaacag ccgaaaatc gaaggcaaa 
      gtgcaagta ccagaagct ttgaagaag atctacaac 
      attcagaat aacttcgag tacgacaca gcatccttg 
      ccccttccg ggactctac accaaaagt gccaacacc 
      atacggagc ttcacccat gtggaaagt ccaatcgat 
      gaaaagttc gtgcaccct caccgcttc cgcttgaaa 
      ttcaatatt tcatcaatt cctcgacac gaaaaactc 
      acagcggcc gaaataaaa ctaacccgt gaaacggcc 
      aaaaacaca tcacacccc tttcaacgt gtcttagtc 
      cacgacatt ctccaacct ggtgtcaag ggcctccac 
      ggccccatc acccgagtg attgactca aaagtggtc 
      gactctcga aaaaacacc accgtgagc atcgacgtc 
      ttcccggca gtggctcgc tggatgcaa gaccccaaa 
      accaaccac ggcatcctc attgtggtt tactccatt 
      ggggcgaaa aaatcaccc ccagagaaa cacctaagg 
      ctgcggcgc gacacagcc cccccacag tggtaccaa 
      caccaaccc cttctcttc acctacacc gacgatggt 
      aagaaccag caaaggaca gggacggag ctgacgaaa 
      atgcggccc aaacgacag agttcgcgc aggcaccgg 
      aaaaatctg aaagacccc tgccggaga cggcagatg 
      tacgtcgat ttcggttcc gtggggtgg aacgactgg 
      atcgtggcc cccttgggc tacgacgcg tattattgc 
      gggggcgag tgcgagtac cccattcca gaccacatg 
      aacacgacg aaccacgcc atagtccag agcctggtc 
      aactcgatg aagccgaag gaggtgcca gggccctgc 
      tgcgtgccg acgcagctg gggcagatg tccatgctg 
      tatctgggc agcgacggc agcgtcatc ctaaaaaac 
      tacaaggag atggtggtg gtggggtgc ggctgccga 
      tag
    mRNA sequence
    • >TC008466 RA
      ggatataat tacataaat aacattacg tatcaggtt 
      gcaacacaa aataaataa tgtaaacaa attgtgggc 
      gtgtgtatt ttttgaaat gaaatgatt taatggaag 
      cgtctccga attccagcc tcatcaagc atatttaaa 
      tgaggcaat taagacagc aattggtgt aaatgagta 
      tcacgatca gaagtcaag tctcggtgt gtttgagtg 
      gtcctgtga aaaggaaga gccacaatg gtcccaaca 
      atatccact aagtattgt ttgatagtc gcacgtgtc 
      gtatcctgt accttgctc tgattggtc gtccaagac 
      cccacacct taacgcatg cacaggtag agggtggca 
      tccttcttc atcagtgtt gtcctggac ttgttacca 
      caacgcagt tttcttttc aattcgaaa ctataatag 
      acttgtgca agtgttgtt cgtaaatgt ggtccattg 
      tgggattat tcaagtgat tacaaacaa caggagaat 
      aatcgtatc gcgatgcgt ttgaacatg ctgatatac 
      ctcatagtg gcatgttgt tggggtaaa tctctctcg 
      atacctcaa caaatactc aatgaattc aaatcgaca 
      ctgttgccc cttttcgga ctcaaagaa cagccgaaa 
      atcgaaggc aaagtgcaa gtaccagaa gctttgaag 
      aagatctac aacattcag aataacttc gagtacgac 
      acagcatcc ttgcccctt ccgggactc tacaccaaa 
      agtgccaac accatacgg agcttcacc catgtggaa 
      agtccaatc gatgaaaag ttcgtgcac cctcaccgc 
      ttccgcttg aaattcaat atttcatca attcctcga 
      cacgaaaaa ctcacagcg gccgaaata aaactaacc 
      cgtgaaacg gccaaaaac acatcacac ccctttcaa 
      cgtgtctta gtccacgac attctccaa cctggtgtc 
      aagggcctc cacggcccc atcacccga gtgattgac 
      tcaaaagtg gtcgactct cgaaaaaac accaccgtg 
      agcatcgac gtcttcccg gcagtggct cgctggatg 
      caagacccc aaaaccaac cacggcatc ctcattgtg 
      gtttactcc attggggcg aaaaaatca cccccagag 
      aaacaccta aggctgcgg cgcgacaca gccccccca 
      cagtggtac caacaccaa ccccttctc ttcacctac 
      accgacgat ggtaagaac cagcaaagg acagggacg 
      gagctgacg aaaatgcgg cccaaacga cagagttcg 
      cgcaggcac cggaaaaat ctgaaagac ccctgccgg 
      agacggcag atgtacgtc gatttcggt tccgtgggg 
      tggaacgac tggatcgtg gcccccttg ggctacgac 
      gcgtattat tgcgggggc gagtgcgag taccccatt 
      ccagaccac atgaacacg acgaaccac gccatagtc 
      cagagcctg gtcaactcg atgaagccg aaggaggtg 
      ccagggccc tgctgcgtg ccgacgcag ctggggcag 
      atgtccatg ctgtatctg ggcagcgac ggcagcgtc 
      atcctaaaa aactacaag gagatggtg gtggtgggg 
      tgcggctgc cgatagtgt catctccag atggtgaaa 
      cgatatctg tgatcagga ttggggacc aaaatagtg 
      tctgtgatc ttaaaaact ttgagactg cttgcagct 
      gcatagtcc cagtgagaa ctgactgat tttttgtgc 
      tatttggac tgggtgaag tggaatctg tgattgtaa 
      ttttaattc attgcgaaa ctaatctgc acccttcga 
      gacattcac gtgactgaa acatgctcg tttaatgtc 
      tcaactgca gtcgatgtg aaagttata agtcggaaa 
      tttacttgt attcaaata tcgagtcca tgcaattga 
      gtcaagtgt aatatatta gtacctgga taaggagta 
      tttattgat tattttata ggtatgtgc atattgtac 
      agattaatt tgtaatata acttgcaag cattgtttc 
      ttaacgtta tgtaagctt ttgtacaga ctgtatata 
      ttaaaatat tttattctt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY