| General information | |||
| TC008459 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 10867891..10905725 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008459 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04495 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
- >TC008459 RA
MGGGWNVQH DQFGVPVKK GSEGADDVS SPESMPDYD PPPLRHIDK YIRPECPCL SKRATVALF SCLGFIIMF GMRTSMGVV KLQFQNETS TAGVDAAIF WGYFITQIP GGLIAAAYP ANKLFGLAI GTSSFLNLF VPAIYFHPN MLIIVKVMQ GLVEGVTYP ACHGIMRFW APPLERSRL ATLAFSGCY AGVMFSMPI SSELVKRLG PLSPFYFYG IFGLIWYMA WLWLVFEKP SYHPVIDPK ELYYIENSL GSTNQNYVT PNISNTPWK TFFTSLPCY AIFVANFCR SWNFYLLVL FQVMYFKDA YGVDIEENG FMGALPHLL MTIIVPGGG ILADRLRKK GILNTTQVR KLFNCGGFG MEATFFLIM AYSDTVMQG MTALTIGVA FSGFAISGF NVNHLDIAP RYASILMGI SNGIGTIAG CICPYVVHL VIDGNRTRE QWRYVFIFS AMIHYAGII FYGVFASGE LQDWADPRV EEEKQWNQL NEAVPVKAP NQNNGLLQR QLSGKNNYG SVDTPAPPR PQPPQQPSP AVPFVPSGN PFLSGSNPF RQEQVQPEA QDTYMHGTV FDRTY
- >TC008459 RA
atggggggc ggctggaat gtacaacat gatcagttt ggggttccg gtcaaaaaa ggcagcgaa ggggcagat gacgtcagc tcaccggaa agtatgccg gattatgat ccacctcct ttgagacac atcgacaag tatatccgg ccagaatgt ccgtgtctg tccaagagg gccactgtt gcgcttttt agttgttta ggttttatc attatgttc ggaatgagg accagcatg ggggttgtc aaactccag tttcagaat gagacgtcg actgccgga gtggacgct gcgattttc tggggttac ttcatcacc caaattccg ggtggtcta atcgctgcg gcttacccc gccaacaag cttttcggc ttagctatc ggcacatcc tcatttcta aatttattt gttcccgcc atttatttt cacccaaat atgcttatc atcgtaaag gtgatgcaa ggtttagtg gagggagtc acatatcca gcctgtcac ggtatcatg cggttttgg gcgcctcct ctcgaacgt tctcgttta gcaacttta gcatttagc ggctgttat gcaggggtg atgttttcg atgcctata tcaagcgaa ttagtgaaa cgtttaggc cctttatct cccttttat ttctacggc atttttggg ttaatatgg tacatggca tggttgtgg ttggtgttt gaaaaaccc tcgtaccat ccagtcatc gatccgaag gaactctac tacattgaa aattcctta gggagcacc aaccaaaac tatgtcact cccaacatc tccaacact ccctggaag acgtttttt acttcgctg ccttgctac gctatattt gtagcgaat ttttgcaga tcttggaat ttttacctc ctggtcttg ttccaagtt atgtacttt aaggacgct tacggagtc gatatcgaa gagaatggc ttcatgggg gctcttccc cacttacta atgacaata atcgtccct ggtggaggc attctcgcc gatcgtctt cgcaagaag ggaattcta aacaccacc caagtgaga aaactcttc aactgcggt ggtttcggc atggaggcc actttcttc ctaattatg gcctattcg gacactgta atgcagggt atgacagcg ttgacgatc ggcgtagct tttagcggc tttgccatt tctgggttc aacgtcaac cacttggat atcgcccca cggtatgcc agcatcctg atgggtatt tcgaacggg attggaacc atcgctggg tgtatttgt ccctacgtc gttcattta gtcatcgac ggaaatcgg actcgcgag caatggcga tacgttttc atcttttcg gccatgatc cactacgca ggcattatt ttttacgga gttttcgct tccggagag cttcaagac tgggccgat ccgcgtgtt gaggaagaa aagcaatgg aatcaactg aatgaagca gttccagtg aaagcaccg aatcaaaat aatggtctg ctgcaacgt caattgagc ggaaaaaat aactacgga tctgtggat acaccagca ccgccacgg ccccaaccc ccacaacaa ccgtcccca gcggtgccc ttcgtacct agcggaaac cccttttta tcggggagt aacccattt aggcaagaa caggtacaa ccagaagct caggacact tatatgcac ggtaccgtt tttgatcgc acttattaa
- >TC008459 RA
gggctccaa tggcgaggg cggcgtgga gacggcgcc aaaacgtct tcccggagc actccgggg accaaacgt accccggac ctgacgtcg tttgcctga tgctgcaac aatcgagac tatggcgac ccaacggcc acacgttgt aatgagtgt ttagtgaga gctcggtta gtggccaat ttagcgaaa ttggaaatt aacaacaca aaaaaacgc aatgggggg cggctggaa tgtacaaca tgatcagtt tggggttcc ggtcaaaaa aggcagcga aggggcaga tgacgtcag ctcaccgga aagtatgcc ggattatga tccacctcc tttgagaca catcgacaa gtatatccg gccagaatg tccgtgtct gtccaagag ggccactgt tgcgctttt tagttgttt aggttttat cattatgtt cggaatgag gaccagcat gggggttgt caaactcca gtttcagaa tgagacgtc gactgccgg agtggacgc tgcgatttt ctggggtta cttcatcac ccaaattcc gggtggtct aatcgctgc ggcttaccc cgccaacaa gcttttcgg cttagctat cggcacatc ctcatttct aaatttatt tgttcccgc catttattt tcacccaaa tatgcttat catcgtaaa ggtgatgca aggtttagt ggagggagt cacatatcc agcctgtca cggtatcat gcggttttg ggcgcctcc tctcgaacg ttctcgttt agcaacttt agcatttag cggctgtta tgcaggggt gatgttttc gatgcctat atcaagcga attagtgaa acgtttagg ccctttatc tccctttta tttctacgg catttttgg gttaatatg gtacatggc atggttgtg gttggtgtt tgaaaaacc ctcgtacca tccagtcat cgatccgaa ggaactcta ctacattga aaattcctt agggagcac caaccaaaa ctatgtcac tcccaacat ctccaacac tccctggaa gacgttttt tacttcgct gccttgcta cgctatatt tgtagcgaa tttttgcag atcttggaa tttttacct cctggtctt gttccaagt tatgtactt taaggacgc ttacggagt cgatatcga agagaatgg cttcatggg ggctcttcc ccacttact aatgacaat aatcgtccc tggtggagg cattctcgc cgatcgtct tcgcaagaa gggaattct aaacaccac ccaagtgag aaaactctt caactgcgg tggtttcgg catggaggc cactttctt cctaattat ggcctattc ggacactgt aatgcaggg tatgacagc gttgacgat cggcgtagc ttttagcgg ctttgccat ttctgggtt caacgtcaa ccacttgga tatcgcccc acggtatgc cagcatcct gatgggtat ttcgaacgg gattggaac catcgctgg gtgtatttg tccctacgt cgttcattt agtcatcga cggaaatcg gactcgcga gcaatggcg atacgtttt catcttttc ggccatgat ccactacgc aggcattat tttttacgg agttttcgc ttccggaga gcttcaaga ctgggccga tccgcgtgt tgaggaaga aaagcaatg gaatcaact gaatgaagc agttccagt gaaagcacc gaatcaaaa taatggtct gctgcaacg tcaattgag cggaaaaaa taactacgg atctgtgga tacaccagc accgccacg gccccaacc cccacaaca accgtcccc agcggtgcc cttcgtacc tagcggaaa ccccttttt atcggggag taacccatt taggcaaga acaggtaca accagaagc tcaggacac ttatatgca cggtaccgt ttttgatcg cacttatta aggtagaaa gtagaatga tcgtcaaaa cttttttaa ggattcaat ttgtttggt tgattacaa taatcccat ttcaacgac taggtgaac aaataggtg ataaaaaac agtttctta tttttgaca gttatttaa aaaggtgtc ggaggataa tttagtaat agaataatg tacagaaag ctatcactg aaactggaa attgtgaaa taaattttt ggagaatat tttgtaaaa actgcaatt gttagtcga ggcactttt tttaacaaa cttacctac atctaggta gatagaaaa atttgtcaa tttgtatag tactttgtt accactgaa aataactat aaaacgcat ttatcatag attaacttg tttctttat cagtaaaga aagatttaa ttattaatt aattaaaga ggagaaact acgaggcaa aaataatct tgatatatt tagaactaa acagatgat tcgtgttat cactatatt ttatcctga atttcggga ggttttcag agaggacta cgacttggc tcacattta tcgctttgt gtgcaacgt gaaaaatag tcttttatt acacagtag ttccgccgg tctatttgc aaaactccc aattctctc ttgaaaact tcccacttg taacaagat tctgaatcc aggattgcg ttatgttgt aaataatca tgtctataa ttccatttt gtttcattt catcatgca ttatataga atgttaagt atttgagga aacttaccc tcaaggctg tgttgacaa taatacggt ggggtctcg caccccacc acaaatcgt tgataataa tggcgataa taattagtt atactgaat gaatgtgcg agctggtat acaaaagga gtgaagcta cataacggc acaaagcag taacgtggg cgcgaagtt agactagag tgttgttac tttgtttcg aaaaacttg ccagttttt ggaaatgat gtaaggctt tctgtaact tctaagtag gtgtagtaa ggaaaaatt acatttgat tatatattt tatttgcag tgttgtttc aacacgact cattttgtc gctaaattg tgaaagacg tgtatgaaa tcgtgtaaa aattaaatg ggtgttaga tgttttcta tgttccttc tactccttc actgtctct ctctaactt gtcaataat tgcgtaaat gtataaaaa tgtatttct tgtttgcga ttttttcga gcggtattg aactgtgtt tagttccgg atgagcaat acctgcttc taggctaca tttaccgtt gttttttcc attcttacc attctcgac gcgcattaa attcgccaa aacttgacc tttgggtga atttcattc gcgctgaga gcagtagtg tagaggcaa taaatgatg caaatattc atgttaaat ttagtgttt ttaaaacct tgcatatct atgtgtttt aataggacc taagtgata tacggcgta aattactaa caaatgcat gctacagac aatatcaaa taatataat gttgacagt tcttacatt cggttgcgt tctgttcac ttagtgaag ttatcatcg atgtgttga atctttaaa atgtgattg tttacaggt ctatgtaaa aatcaaaaa tgtataata aataattct gttactg
