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Gene information
General information
TC008459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10867891..10905725
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008459
    iBeetle screen iB_04495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008459 RA
      MGGGWNVQH DQFGVPVKK GSEGADDVS SPESMPDYD 
      PPPLRHIDK YIRPECPCL SKRATVALF SCLGFIIMF 
      GMRTSMGVV KLQFQNETS TAGVDAAIF WGYFITQIP 
      GGLIAAAYP ANKLFGLAI GTSSFLNLF VPAIYFHPN 
      MLIIVKVMQ GLVEGVTYP ACHGIMRFW APPLERSRL 
      ATLAFSGCY AGVMFSMPI SSELVKRLG PLSPFYFYG 
      IFGLIWYMA WLWLVFEKP SYHPVIDPK ELYYIENSL 
      GSTNQNYVT PNISNTPWK TFFTSLPCY AIFVANFCR 
      SWNFYLLVL FQVMYFKDA YGVDIEENG FMGALPHLL 
      MTIIVPGGG ILADRLRKK GILNTTQVR KLFNCGGFG 
      MEATFFLIM AYSDTVMQG MTALTIGVA FSGFAISGF 
      NVNHLDIAP RYASILMGI SNGIGTIAG CICPYVVHL 
      VIDGNRTRE QWRYVFIFS AMIHYAGII FYGVFASGE 
      LQDWADPRV EEEKQWNQL NEAVPVKAP NQNNGLLQR 
      QLSGKNNYG SVDTPAPPR PQPPQQPSP AVPFVPSGN 
      PFLSGSNPF RQEQVQPEA QDTYMHGTV FDRTY
    CDS sequence
    • >TC008459 RA
      atggggggc ggctggaat gtacaacat gatcagttt 
      ggggttccg gtcaaaaaa ggcagcgaa ggggcagat 
      gacgtcagc tcaccggaa agtatgccg gattatgat 
      ccacctcct ttgagacac atcgacaag tatatccgg 
      ccagaatgt ccgtgtctg tccaagagg gccactgtt 
      gcgcttttt agttgttta ggttttatc attatgttc 
      ggaatgagg accagcatg ggggttgtc aaactccag 
      tttcagaat gagacgtcg actgccgga gtggacgct 
      gcgattttc tggggttac ttcatcacc caaattccg 
      ggtggtcta atcgctgcg gcttacccc gccaacaag 
      cttttcggc ttagctatc ggcacatcc tcatttcta 
      aatttattt gttcccgcc atttatttt cacccaaat 
      atgcttatc atcgtaaag gtgatgcaa ggtttagtg 
      gagggagtc acatatcca gcctgtcac ggtatcatg 
      cggttttgg gcgcctcct ctcgaacgt tctcgttta 
      gcaacttta gcatttagc ggctgttat gcaggggtg 
      atgttttcg atgcctata tcaagcgaa ttagtgaaa 
      cgtttaggc cctttatct cccttttat ttctacggc 
      atttttggg ttaatatgg tacatggca tggttgtgg 
      ttggtgttt gaaaaaccc tcgtaccat ccagtcatc 
      gatccgaag gaactctac tacattgaa aattcctta 
      gggagcacc aaccaaaac tatgtcact cccaacatc 
      tccaacact ccctggaag acgtttttt acttcgctg 
      ccttgctac gctatattt gtagcgaat ttttgcaga 
      tcttggaat ttttacctc ctggtcttg ttccaagtt 
      atgtacttt aaggacgct tacggagtc gatatcgaa 
      gagaatggc ttcatgggg gctcttccc cacttacta 
      atgacaata atcgtccct ggtggaggc attctcgcc 
      gatcgtctt cgcaagaag ggaattcta aacaccacc 
      caagtgaga aaactcttc aactgcggt ggtttcggc 
      atggaggcc actttcttc ctaattatg gcctattcg 
      gacactgta atgcagggt atgacagcg ttgacgatc 
      ggcgtagct tttagcggc tttgccatt tctgggttc 
      aacgtcaac cacttggat atcgcccca cggtatgcc 
      agcatcctg atgggtatt tcgaacggg attggaacc 
      atcgctggg tgtatttgt ccctacgtc gttcattta 
      gtcatcgac ggaaatcgg actcgcgag caatggcga 
      tacgttttc atcttttcg gccatgatc cactacgca 
      ggcattatt ttttacgga gttttcgct tccggagag 
      cttcaagac tgggccgat ccgcgtgtt gaggaagaa 
      aagcaatgg aatcaactg aatgaagca gttccagtg 
      aaagcaccg aatcaaaat aatggtctg ctgcaacgt 
      caattgagc ggaaaaaat aactacgga tctgtggat 
      acaccagca ccgccacgg ccccaaccc ccacaacaa 
      ccgtcccca gcggtgccc ttcgtacct agcggaaac 
      cccttttta tcggggagt aacccattt aggcaagaa 
      caggtacaa ccagaagct caggacact tatatgcac 
      ggtaccgtt tttgatcgc acttattaa
    mRNA sequence
    • >TC008459 RA
      gggctccaa tggcgaggg cggcgtgga gacggcgcc 
      aaaacgtct tcccggagc actccgggg accaaacgt 
      accccggac ctgacgtcg tttgcctga tgctgcaac 
      aatcgagac tatggcgac ccaacggcc acacgttgt 
      aatgagtgt ttagtgaga gctcggtta gtggccaat 
      ttagcgaaa ttggaaatt aacaacaca aaaaaacgc 
      aatgggggg cggctggaa tgtacaaca tgatcagtt 
      tggggttcc ggtcaaaaa aggcagcga aggggcaga 
      tgacgtcag ctcaccgga aagtatgcc ggattatga 
      tccacctcc tttgagaca catcgacaa gtatatccg 
      gccagaatg tccgtgtct gtccaagag ggccactgt 
      tgcgctttt tagttgttt aggttttat cattatgtt 
      cggaatgag gaccagcat gggggttgt caaactcca 
      gtttcagaa tgagacgtc gactgccgg agtggacgc 
      tgcgatttt ctggggtta cttcatcac ccaaattcc 
      gggtggtct aatcgctgc ggcttaccc cgccaacaa 
      gcttttcgg cttagctat cggcacatc ctcatttct 
      aaatttatt tgttcccgc catttattt tcacccaaa 
      tatgcttat catcgtaaa ggtgatgca aggtttagt 
      ggagggagt cacatatcc agcctgtca cggtatcat 
      gcggttttg ggcgcctcc tctcgaacg ttctcgttt 
      agcaacttt agcatttag cggctgtta tgcaggggt 
      gatgttttc gatgcctat atcaagcga attagtgaa 
      acgtttagg ccctttatc tccctttta tttctacgg 
      catttttgg gttaatatg gtacatggc atggttgtg 
      gttggtgtt tgaaaaacc ctcgtacca tccagtcat 
      cgatccgaa ggaactcta ctacattga aaattcctt 
      agggagcac caaccaaaa ctatgtcac tcccaacat 
      ctccaacac tccctggaa gacgttttt tacttcgct 
      gccttgcta cgctatatt tgtagcgaa tttttgcag 
      atcttggaa tttttacct cctggtctt gttccaagt 
      tatgtactt taaggacgc ttacggagt cgatatcga 
      agagaatgg cttcatggg ggctcttcc ccacttact 
      aatgacaat aatcgtccc tggtggagg cattctcgc 
      cgatcgtct tcgcaagaa gggaattct aaacaccac 
      ccaagtgag aaaactctt caactgcgg tggtttcgg 
      catggaggc cactttctt cctaattat ggcctattc 
      ggacactgt aatgcaggg tatgacagc gttgacgat 
      cggcgtagc ttttagcgg ctttgccat ttctgggtt 
      caacgtcaa ccacttgga tatcgcccc acggtatgc 
      cagcatcct gatgggtat ttcgaacgg gattggaac 
      catcgctgg gtgtatttg tccctacgt cgttcattt 
      agtcatcga cggaaatcg gactcgcga gcaatggcg 
      atacgtttt catcttttc ggccatgat ccactacgc 
      aggcattat tttttacgg agttttcgc ttccggaga 
      gcttcaaga ctgggccga tccgcgtgt tgaggaaga 
      aaagcaatg gaatcaact gaatgaagc agttccagt 
      gaaagcacc gaatcaaaa taatggtct gctgcaacg 
      tcaattgag cggaaaaaa taactacgg atctgtgga 
      tacaccagc accgccacg gccccaacc cccacaaca 
      accgtcccc agcggtgcc cttcgtacc tagcggaaa 
      ccccttttt atcggggag taacccatt taggcaaga 
      acaggtaca accagaagc tcaggacac ttatatgca 
      cggtaccgt ttttgatcg cacttatta aggtagaaa 
      gtagaatga tcgtcaaaa cttttttaa ggattcaat 
      ttgtttggt tgattacaa taatcccat ttcaacgac 
      taggtgaac aaataggtg ataaaaaac agtttctta 
      tttttgaca gttatttaa aaaggtgtc ggaggataa 
      tttagtaat agaataatg tacagaaag ctatcactg 
      aaactggaa attgtgaaa taaattttt ggagaatat 
      tttgtaaaa actgcaatt gttagtcga ggcactttt 
      tttaacaaa cttacctac atctaggta gatagaaaa 
      atttgtcaa tttgtatag tactttgtt accactgaa 
      aataactat aaaacgcat ttatcatag attaacttg 
      tttctttat cagtaaaga aagatttaa ttattaatt 
      aattaaaga ggagaaact acgaggcaa aaataatct 
      tgatatatt tagaactaa acagatgat tcgtgttat 
      cactatatt ttatcctga atttcggga ggttttcag 
      agaggacta cgacttggc tcacattta tcgctttgt 
      gtgcaacgt gaaaaatag tcttttatt acacagtag 
      ttccgccgg tctatttgc aaaactccc aattctctc 
      ttgaaaact tcccacttg taacaagat tctgaatcc 
      aggattgcg ttatgttgt aaataatca tgtctataa 
      ttccatttt gtttcattt catcatgca ttatataga 
      atgttaagt atttgagga aacttaccc tcaaggctg 
      tgttgacaa taatacggt ggggtctcg caccccacc 
      acaaatcgt tgataataa tggcgataa taattagtt 
      atactgaat gaatgtgcg agctggtat acaaaagga 
      gtgaagcta cataacggc acaaagcag taacgtggg 
      cgcgaagtt agactagag tgttgttac tttgtttcg 
      aaaaacttg ccagttttt ggaaatgat gtaaggctt 
      tctgtaact tctaagtag gtgtagtaa ggaaaaatt 
      acatttgat tatatattt tatttgcag tgttgtttc 
      aacacgact cattttgtc gctaaattg tgaaagacg 
      tgtatgaaa tcgtgtaaa aattaaatg ggtgttaga 
      tgttttcta tgttccttc tactccttc actgtctct 
      ctctaactt gtcaataat tgcgtaaat gtataaaaa 
      tgtatttct tgtttgcga ttttttcga gcggtattg 
      aactgtgtt tagttccgg atgagcaat acctgcttc 
      taggctaca tttaccgtt gttttttcc attcttacc 
      attctcgac gcgcattaa attcgccaa aacttgacc 
      tttgggtga atttcattc gcgctgaga gcagtagtg 
      tagaggcaa taaatgatg caaatattc atgttaaat 
      ttagtgttt ttaaaacct tgcatatct atgtgtttt 
      aataggacc taagtgata tacggcgta aattactaa 
      caaatgcat gctacagac aatatcaaa taatataat 
      gttgacagt tcttacatt cggttgcgt tctgttcac 
      ttagtgaag ttatcatcg atgtgttga atctttaaa 
      atgtgattg tttacaggt ctatgtaaa aatcaaaaa 
      tgtataata aataattct gttactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY