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Gene information
General information
TC008459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10867891..10905725
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008459
    iBeetle screen iB_04495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008459 RA
      MGGGWNVQH DQFGVPVKK GSEGADDVS SPESMPDYD 
PPPLRHIDK YIRPECPCL SKRATVALF SCLGFIIMF 
GMRTSMGVV KLQFQNETS TAGVDAAIF WGYFITQIP 
GGLIAAAYP ANKLFGLAI GTSSFLNLF VPAIYFHPN 
MLIIVKVMQ GLVEGVTYP ACHGIMRFW APPLERSRL 
ATLAFSGCY AGVMFSMPI SSELVKRLG PLSPFYFYG 
IFGLIWYMA WLWLVFEKP SYHPVIDPK ELYYIENSL 
GSTNQNYVT PNISNTPWK TFFTSLPCY AIFVANFCR 
SWNFYLLVL FQVMYFKDA YGVDIEENG FMGALPHLL 
MTIIVPGGG ILADRLRKK GILNTTQVR KLFNCGGFG 
MEATFFLIM AYSDTVMQG MTALTIGVA FSGFAISGF 
NVNHLDIAP RYASILMGI SNGIGTIAG CICPYVVHL 
VIDGNRTRE QWRYVFIFS AMIHYAGII FYGVFASGE 
LQDWADPRV EEEKQWNQL NEAVPVKAP NQNNGLLQR 
QLSGKNNYG SVDTPAPPR PQPPQQPSP AVPFVPSGN 
PFLSGSNPF RQEQVQPEA QDTYMHGTV FDRTY
    CDS sequence
    • >TC008459 RA
      atggggggc ggctggaat gtacaacat gatcagttt 
ggggttccg gtcaaaaaa ggcagcgaa ggggcagat 
gacgtcagc tcaccggaa agtatgccg gattatgat 
ccacctcct ttgagacac atcgacaag tatatccgg 
ccagaatgt ccgtgtctg tccaagagg gccactgtt 
gcgcttttt agttgttta ggttttatc attatgttc 
ggaatgagg accagcatg ggggttgtc aaactccag 
tttcagaat gagacgtcg actgccgga gtggacgct 
gcgattttc tggggttac ttcatcacc caaattccg 
ggtggtcta atcgctgcg gcttacccc gccaacaag 
cttttcggc ttagctatc ggcacatcc tcatttcta 
aatttattt gttcccgcc atttatttt cacccaaat 
atgcttatc atcgtaaag gtgatgcaa ggtttagtg 
gagggagtc acatatcca gcctgtcac ggtatcatg 
cggttttgg gcgcctcct ctcgaacgt tctcgttta 
gcaacttta gcatttagc ggctgttat gcaggggtg 
atgttttcg atgcctata tcaagcgaa ttagtgaaa 
cgtttaggc cctttatct cccttttat ttctacggc 
atttttggg ttaatatgg tacatggca tggttgtgg 
ttggtgttt gaaaaaccc tcgtaccat ccagtcatc 
gatccgaag gaactctac tacattgaa aattcctta 
gggagcacc aaccaaaac tatgtcact cccaacatc 
tccaacact ccctggaag acgtttttt acttcgctg 
ccttgctac gctatattt gtagcgaat ttttgcaga 
tcttggaat ttttacctc ctggtcttg ttccaagtt 
atgtacttt aaggacgct tacggagtc gatatcgaa 
gagaatggc ttcatgggg gctcttccc cacttacta 
atgacaata atcgtccct ggtggaggc attctcgcc 
gatcgtctt cgcaagaag ggaattcta aacaccacc 
caagtgaga aaactcttc aactgcggt ggtttcggc 
atggaggcc actttcttc ctaattatg gcctattcg 
gacactgta atgcagggt atgacagcg ttgacgatc 
ggcgtagct tttagcggc tttgccatt tctgggttc 
aacgtcaac cacttggat atcgcccca cggtatgcc 
agcatcctg atgggtatt tcgaacggg attggaacc 
atcgctggg tgtatttgt ccctacgtc gttcattta 
gtcatcgac ggaaatcgg actcgcgag caatggcga 
tacgttttc atcttttcg gccatgatc cactacgca 
ggcattatt ttttacgga gttttcgct tccggagag 
cttcaagac tgggccgat ccgcgtgtt gaggaagaa 
aagcaatgg aatcaactg aatgaagca gttccagtg 
aaagcaccg aatcaaaat aatggtctg ctgcaacgt 
caattgagc ggaaaaaat aactacgga tctgtggat 
acaccagca ccgccacgg ccccaaccc ccacaacaa 
ccgtcccca gcggtgccc ttcgtacct agcggaaac 
cccttttta tcggggagt aacccattt aggcaagaa 
caggtacaa ccagaagct caggacact tatatgcac 
ggtaccgtt tttgatcgc acttattaa
    mRNA sequence
    • >TC008459 RA
      gggctccaa tggcgaggg cggcgtgga gacggcgcc 
aaaacgtct tcccggagc actccgggg accaaacgt 
accccggac ctgacgtcg tttgcctga tgctgcaac 
aatcgagac tatggcgac ccaacggcc acacgttgt 
aatgagtgt ttagtgaga gctcggtta gtggccaat 
ttagcgaaa ttggaaatt aacaacaca aaaaaacgc 
aatgggggg cggctggaa tgtacaaca tgatcagtt 
tggggttcc ggtcaaaaa aggcagcga aggggcaga 
tgacgtcag ctcaccgga aagtatgcc ggattatga 
tccacctcc tttgagaca catcgacaa gtatatccg 
gccagaatg tccgtgtct gtccaagag ggccactgt 
tgcgctttt tagttgttt aggttttat cattatgtt 
cggaatgag gaccagcat gggggttgt caaactcca 
gtttcagaa tgagacgtc gactgccgg agtggacgc 
tgcgatttt ctggggtta cttcatcac ccaaattcc 
gggtggtct aatcgctgc ggcttaccc cgccaacaa 
gcttttcgg cttagctat cggcacatc ctcatttct 
aaatttatt tgttcccgc catttattt tcacccaaa 
tatgcttat catcgtaaa ggtgatgca aggtttagt 
ggagggagt cacatatcc agcctgtca cggtatcat 
gcggttttg ggcgcctcc tctcgaacg ttctcgttt 
agcaacttt agcatttag cggctgtta tgcaggggt 
gatgttttc gatgcctat atcaagcga attagtgaa 
acgtttagg ccctttatc tccctttta tttctacgg 
catttttgg gttaatatg gtacatggc atggttgtg 
gttggtgtt tgaaaaacc ctcgtacca tccagtcat 
cgatccgaa ggaactcta ctacattga aaattcctt 
agggagcac caaccaaaa ctatgtcac tcccaacat 
ctccaacac tccctggaa gacgttttt tacttcgct 
gccttgcta cgctatatt tgtagcgaa tttttgcag 
atcttggaa tttttacct cctggtctt gttccaagt 
tatgtactt taaggacgc ttacggagt cgatatcga 
agagaatgg cttcatggg ggctcttcc ccacttact 
aatgacaat aatcgtccc tggtggagg cattctcgc 
cgatcgtct tcgcaagaa gggaattct aaacaccac 
ccaagtgag aaaactctt caactgcgg tggtttcgg 
catggaggc cactttctt cctaattat ggcctattc 
ggacactgt aatgcaggg tatgacagc gttgacgat 
cggcgtagc ttttagcgg ctttgccat ttctgggtt 
caacgtcaa ccacttgga tatcgcccc acggtatgc 
cagcatcct gatgggtat ttcgaacgg gattggaac 
catcgctgg gtgtatttg tccctacgt cgttcattt 
agtcatcga cggaaatcg gactcgcga gcaatggcg 
atacgtttt catcttttc ggccatgat ccactacgc 
aggcattat tttttacgg agttttcgc ttccggaga 
gcttcaaga ctgggccga tccgcgtgt tgaggaaga 
aaagcaatg gaatcaact gaatgaagc agttccagt 
gaaagcacc gaatcaaaa taatggtct gctgcaacg 
tcaattgag cggaaaaaa taactacgg atctgtgga 
tacaccagc accgccacg gccccaacc cccacaaca 
accgtcccc agcggtgcc cttcgtacc tagcggaaa 
ccccttttt atcggggag taacccatt taggcaaga 
acaggtaca accagaagc tcaggacac ttatatgca 
cggtaccgt ttttgatcg cacttatta aggtagaaa 
gtagaatga tcgtcaaaa cttttttaa ggattcaat 
ttgtttggt tgattacaa taatcccat ttcaacgac 
taggtgaac aaataggtg ataaaaaac agtttctta 
tttttgaca gttatttaa aaaggtgtc ggaggataa 
tttagtaat agaataatg tacagaaag ctatcactg 
aaactggaa attgtgaaa taaattttt ggagaatat 
tttgtaaaa actgcaatt gttagtcga ggcactttt 
tttaacaaa cttacctac atctaggta gatagaaaa 
atttgtcaa tttgtatag tactttgtt accactgaa 
aataactat aaaacgcat ttatcatag attaacttg 
tttctttat cagtaaaga aagatttaa ttattaatt 
aattaaaga ggagaaact acgaggcaa aaataatct 
tgatatatt tagaactaa acagatgat tcgtgttat 
cactatatt ttatcctga atttcggga ggttttcag 
agaggacta cgacttggc tcacattta tcgctttgt 
gtgcaacgt gaaaaatag tcttttatt acacagtag 
ttccgccgg tctatttgc aaaactccc aattctctc 
ttgaaaact tcccacttg taacaagat tctgaatcc 
aggattgcg ttatgttgt aaataatca tgtctataa 
ttccatttt gtttcattt catcatgca ttatataga 
atgttaagt atttgagga aacttaccc tcaaggctg 
tgttgacaa taatacggt ggggtctcg caccccacc 
acaaatcgt tgataataa tggcgataa taattagtt 
atactgaat gaatgtgcg agctggtat acaaaagga 
gtgaagcta cataacggc acaaagcag taacgtggg 
cgcgaagtt agactagag tgttgttac tttgtttcg 
aaaaacttg ccagttttt ggaaatgat gtaaggctt 
tctgtaact tctaagtag gtgtagtaa ggaaaaatt 
acatttgat tatatattt tatttgcag tgttgtttc 
aacacgact cattttgtc gctaaattg tgaaagacg 
tgtatgaaa tcgtgtaaa aattaaatg ggtgttaga 
tgttttcta tgttccttc tactccttc actgtctct 
ctctaactt gtcaataat tgcgtaaat gtataaaaa 
tgtatttct tgtttgcga ttttttcga gcggtattg 
aactgtgtt tagttccgg atgagcaat acctgcttc 
taggctaca tttaccgtt gttttttcc attcttacc 
attctcgac gcgcattaa attcgccaa aacttgacc 
tttgggtga atttcattc gcgctgaga gcagtagtg 
tagaggcaa taaatgatg caaatattc atgttaaat 
ttagtgttt ttaaaacct tgcatatct atgtgtttt 
aataggacc taagtgata tacggcgta aattactaa 
caaatgcat gctacagac aatatcaaa taatataat 
gttgacagt tcttacatt cggttgcgt tctgttcac 
ttagtgaag ttatcatcg atgtgttga atctttaaa 
atgtgattg tttacaggt ctatgtaaa aatcaaaaa 
tgtataata aataattct gttactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY