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Gene information
General information
TC008440 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10315877..10317944
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008440
    iBeetle screen iB_04492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008440 RA
      MSDPDSVKF VTDFNKICR TCLSEKNKE DLCSLFDNS 
      LDVILTNLA EINVRRDDG LPGFICNDC IFTLNLFIS 
      FKEQCQKAE SQLRKALYT TPEIYGEYK TVTVLGDNI 
      STIIEFKND SVLDNRDKI ITVSINEPQ IVNSDLQID 
      NPVTTDPLK CPKCERSFK LESTLKTHL LKHEKNQKL 
      KCEICNKEF NLVTQFKHH VRTHTNYKP FSCSKCDKS 
      FALSSSLTK HMRTHGGER KHLCITCGK RFFEPGHLN 
      IHMRTHTGE KPVICNKCG KRFTDPHGL IAHNKIHTG 
      ERKYECNTC GKKFAHSFV LKAHKRVHT GEKPYACTM 
      CKAAFATSS YLTIHKRTH TQERPYKCE CCSKAFISR 
      CALVAHMRT HTGEKKFQC SVCGKRTGR AADLQIHMR 
      SHTGEKPYS CDLCPKKYH TSSNLAAHK RTHLGVKNH 
      FCAVCSKAF GDLRTLKCH MRIHTGERP YICGVCGNR 
      YTQSGQLAA HRRTHDVYI DVNTVT
    CDS sequence
    • >TC008440 RA
      atgtcagac ccagattct gtgaaattt gtaacagat 
      ttcaataaa atttgccgg acatgtctt tcagaaaaa 
      aataaggaa gatttgtgt tctctattt gataattct 
      cttgatgtg attcttaca aatttagct gaaataaat 
      gttagaagg gacgacggt ttgcccggt tttatatgt 
      aacgactgc attttcact ttaaattta tttataagt 
      tttaaagag caatgccag aaggcagag tcccaactg 
      aggaaagct ctgtacact acgccggag atttacggc 
      gagtacaaa actgtaaca gtattaggt gacaacatt 
      tccactatt attgaattc aagaatgac tctgtgtta 
      gacaatcgc gacaaaatc ataacagtg agcataaat 
      gagccccaa attgtaaat agcgacttg caaatcgat 
      aatccagtt acaacggac cccctgaaa tgccccaaa 
      tgcgagcgt tctttcaaa cttgaatcc actctaaaa 
      acccattta ctaaaacac gaaaaaaac caaaaactg 
      aagtgtgaa atttgtaat aaagagttt aatctggtt 
      acacaattc aaacatcac gtgcgcacc cacaccaat 
      tacaaaccc ttttcttgc tcaaaatgc gacaaatca 
      ttcgcatta tcgtcaagc ttgactaaa cacatgcga 
      acacatggc ggcgaacgc aaacacttg tgtatcacc 
      tgcgggaag cggtttttc gaaccgggc caccttaac 
      attcacatg cggacacac accggggaa aaaccggtt 
      atttgtaac aagtgtggt aagagattt acagaccca 
      cacgggctc atagcccac aacaaaatc cacaccggc 
      gaacgcaaa tacgaatgc aacacttgt gggaaaaaa 
      ttcgcacat tctttcgtg cttaaggcg cataaaagg 
      gtccacacc ggggaaaaa ccgtacgct tgcactatg 
      tgcaaggcc gcctttgcg acgtcctcg tacttaacg 
      attcacaaa aggacgcac acgcaagaa cggccctac 
      aagtgcgaa tgttgcagt aaggctttt atcagtcgg 
      tgtgcttta gtagcacac atgaggaca cacacaggg 
      gagaagaaa ttccagtgt tcggtttgt ggtaaacgc 
      acagggcgg gccgctgat ttacagata catatgagg 
      tcacacact ggggaaaaa ccctacagt tgtgacttg 
      tgcccgaaa aaataccac acttcgagt aacttagcg 
      gcgcataaa aggacacat ctgggggtc aaaaaccac 
      ttttgcgcc gtttgtagc aaggctttt ggcgacttg 
      aggaccctt aagtgtcac atgaggata cacacgggg 
      gaaagaccg tacatttgc ggggtgtgc ggcaatagg 
      tacacccag tcgggacaa ttggccgca catagacgc 
      acgcatgat gtttacatt gatgtaaat actgttacg 
      taa
    mRNA sequence
    • >TC008440 RA
      ttgaaatga aactgaaca accccgaca acagcacca 
      ccaaccctt tagactaaa aaattatta taagtttat 
      tatatgcaa ccaataata catccatgt gaccaacag 
      ttttgtgac agctgtgac ccacctttg tttatgtgt 
      cttaaatgg gggcttatt cagcttaaa atagcgaaa 
      gttgggttt catttgaag taagatcat gtcagaccc 
      agattctgt gaaatttgt aacagattt caataaaat 
      ttgccggac atgtctttc agaaaaaaa taaggaaga 
      tttgtgttc tctatttga taattctct tgatgtgat 
      tcttacaaa tttagctga aataaatgt tagaaggga 
      cgacggttt gcccggttt tatatgtaa cgactgcat 
      tttcacttt aaatttatt tataagttt taaagagca 
      atgccagaa ggcagagtc ccaactgag gaaagctct 
      gtacactac gccggagat ttacggcga gtacaaaac 
      tgtaacagt attaggtga caacatttc cactattat 
      tgaattcaa gaatgactc tgtgttaga caatcgcga 
      caaaatcat aacagtgag cataaatga gccccaaat 
      tgtaaatag cgacttgca aatcgataa tccagttac 
      aacggaccc cctgaaatg ccccaaatg cgagcgttc 
      tttcaaact tgaatccac tctaaaaac ccatttact 
      aaaacacga aaaaaacca aaaactgaa gtgtgaaat 
      ttgtaataa agagtttaa tctggttac acaattcaa 
      acatcacgt gcgcaccca caccaatta caaaccctt 
      ttcttgctc aaaatgcga caaatcatt cgcattatc 
      gtcaagctt gactaaaca catgcgaac acatggcgg 
      cgaacgcaa acacttgtg tatcacctg cgggaagcg 
      gtttttcga accgggcca ccttaacat tcacatgcg 
      gacacacac cggggaaaa accggttat ttgtaacaa 
      gtgtggtaa gagatttac agacccaca cgggctcat 
      agcccacaa caaaatcca caccggcga acgcaaata 
      cgaatgcaa cacttgtgg gaaaaaatt cgcacattc 
      tttcgtgct taaggcgca taaaagggt ccacaccgg 
      ggaaaaacc gtacgcttg cactatgtg caaggccgc 
      ctttgcgac gtcctcgta cttaacgat tcacaaaag 
      gacgcacac gcaagaacg gccctacaa gtgcgaatg 
      ttgcagtaa ggcttttat cagtcggtg tgctttagt 
      agcacacat gaggacaca cacagggga gaagaaatt 
      ccagtgttc ggtttgtgg taaacgcac agggcgggc 
      cgctgattt acagataca tatgaggtc acacactgg 
      ggaaaaacc ctacagttg tgacttgtg cccgaaaaa 
      ataccacac ttcgagtaa cttagcggc gcataaaag 
      gacacatct gggggtcaa aaaccactt ttgcgccgt 
      ttgtagcaa ggcttttgg cgacttgag gacccttaa 
      gtgtcacat gaggataca cacggggga aagaccgta 
      catttgcgg ggtgtgcgg caataggta cacccagtc 
      gggacaatt ggccgcaca tagacgcac gcatgatgt 
      ttacattga tgtaaatac tgttacgta aataaatca 
      ctgaaaacg actggaata tttatttag ggaagcccc 
      ccgatcacg cgccccctg gtggcggcg tcggtaatt 
      aacaaatcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY