Status
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Gene information
General information
TC008439 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10307137..10312637
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008439
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04491

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008439 RA
      MWWLCKIIF FGSVVASFA QTKKPNIIV IVADDMGFN 
      DVGFHGSNE IPTPNIDAL AYNGVILNS HYTQALCTP 
      SRSAFLTGK YPIHLGMQH LVILEPEPW GLPLNETIL 
      PQYLKRNGY ATHAIGKWH LGFFRKEYT PTYRGFDSH 
      FGYWQGLQD YYKHTVHAT FTPEHGYDM RRNMTVDWS 
      AQGKYSTTL FTDEAVRLI REHNTENPM FMYLAHLAP 
      HSGNDDDPL QAPDEEIAK FGHIADPER RIYAAMVSM 
      LDKSVGSVI AALRDKHML ENSIIVFMS DNGAKPDGI 
      HANHGSNYP LRGNKNSAW EGAMRCVAA IWSPLIKKP 
      QRVSNSLMH ISDWLPTFY TAAGLNKTE LPKMDGVDM 
      WASISEGKD SPRTELLHN IDEIYNYGA LRVGNWKYL 
      YGSTTNGKS DGWYGSSGR DPLYTYDDS AVLASQTGS 
      TLAGLTTYQ QIKEKHQGD TNFTHKLLD SETIKTLRG 
      AAEVKCPRV NFEEIPESK KCNAVESPC LFNIKEDPC 
      EQINLAAER PMIVLNMEM ALARFKQTA LPIRNVPRD 
      PNADPAKWN NTWVNWQDY EDVKKQKIY FNTLSPLAI 
      GLISAAVVA FVVVIIILV TITVKSSLD KKKAGKAFF 
      DDPMEQMMT MAPKPQIFE DRELQNRES IRNEFRTVE
    CDS sequence
    • >TC008439 RA
      atgtggtgg ctgtgcaaa ataattttc ttcggtagt 
      gtggtggca agtttcgct caaaccaaa aaacccaac 
      attattgtg attgtggcc gacgacatg gggttcaac 
      gatgtcggt tttcacgga agcaacgaa attccaacc 
      cctaatatc gacgctttg gcctacaac ggcgtcatc 
      ctcaacagc cactacacc caggccttg tgcaccccc 
      tcacgttct gccttcctc actgggaag taccccatc 
      catctcgga atgcaacat ctcgttata cttgaacct 
      gaaccgtgg ggactgcct ctaaacgag accatttta 
      ccgcaatat ctcaagagg aatggatat gccacacat 
      gccataggt aaatggcat ttgggcttc ttccgaaag 
      gaatatacg ccgacctat agggggttc gattcgcat 
      tttgggtac tggcaaggg ttgcaggac tactacaaa 
      catacggtg cacgcaacg tttacacct gagcacggt 
      tacgacatg cgacgtaac atgaccgta gactggagc 
      gcccagggc aagtactcc acgactctc ttcaccgac 
      gaagccgtc cgtctaatc cgcgagcac aacaccgaa 
      aaccccatg ttcatgtac ctcgcccac ttggcccca 
      cactcgggc aacgacgac gaccctctg caagccccc 
      gacgaagaa atcgcgaaa ttcggacac atcgcagac 
      cccgaacgt cgaatttac gccgccatg gtctccatg 
      ttggacaag agtgtgggc agtgtgata gccgctttg 
      agggacaaa cacatgctg gaaaattcg attattgtg 
      ttcatgtca gacaacggg gcaaagccc gatggaatc 
      cacgccaat cacggctcg aattatcct ttgcggggc 
      aacaagaac tcggcttgg gaaggggcc atgaggtgc 
      gtggcggcg atctggagc ccccttatc aagaaaccg 
      caacgtgtc tcgaacagt ctgatgcac atttcggac 
      tggttaccg actttctac acagctgca gggctcaat 
      aagaccgaa ctaccgaaa atggacggg gttgacatg 
      tgggcctcc atttccgaa gggaaagac agcccccgg 
      actgaactc ttacacaac attgacgaa atttacaat 
      tatggggca cttagagtc gggaattgg aagtatctc 
      tacggatcc accacaaat ggtaaaagt gatggttgg 
      tatgggtct tcaggtcgt gaccccctt tacacttac 
      gatgatagc gccgttttg gcctcacag accggctcg 
      acacttgcc ggtcttaca acctaccaa caaatcaag 
      gaaaaacac caaggtgac actaatttc acacacaaa 
      ctgctcgac agtgagacg atcaagacc cttcggggc 
      gcagccgaa gtcaagtgt ccgcgagtc aattttgag 
      gaaatcccc gaaagtaag aaatgtaac gcagtcgag 
      tccccttgt ctctttaac atcaaggaa gatccctgc 
      gaacaaatc aatttagcg gccgaacgt cccatgatt 
      gtcctgaac atggaaatg gcattagcc cgattcaaa 
      caaacggct ctacccatc agaaacgtc ccaagagac 
      cctaatgcg gaccccgcc aagtggaac aacacttgg 
      gtcaattgg caagactac gaagacgtg aaaaagcag 
      aaaatttac ttcaatacg ttgtcaccg ctggcgatt 
      ggcttaatt tccgcagcg gttgttgcc tttgttgtc 
      gttataatt atcctggtg actatcaca gtgaagagt 
      tctttggat aagaaaaag gccgggaaa gcgtttttc 
      gacgatccc atggagcaa atgatgacg atggcgccg 
      aaaccacaa attttcgaa gacagggaa ttgcaaaat 
      agggagagt atcagaaat gagtttagg acggtggaa 
      tag
    mRNA sequence
    • >TC008439 RA
      ttagacaca ttgcaaaag cccgaccaa caactaccc 
      tcccaaaat gtggtggct gtgcaaaat aattttctt 
      cggtagtgt ggtggcaag tttcgctca aaccaaaaa 
      acccaacat tattgtgat tgtggccga cgacatggg 
      gttcaacga tgtcggttt tcacggaag caacgaaat 
      tccaacccc taatatcga cgctttggc ctacaacgg 
      cgtcatcct caacagcca ctacaccca ggccttgtg 
      caccccctc acgttctgc cttcctcac tgggaagta 
      ccccatcca tctcggaat gcaacatct cgttatact 
      tgaacctga accgtgggg actgcctct aaacgagac 
      cattttacc gcaatatct caagaggaa tggatatgc 
      cacacatgc cataggtaa atggcattt gggcttctt 
      ccgaaagga atatacgcc gacctatag ggggttcga 
      ttcgcattt tgggtactg gcaagggtt gcaggacta 
      ctacaaaca tacggtgca cgcaacgtt tacacctga 
      gcacggtta cgacatgcg acgtaacat gaccgtaga 
      ctggagcgc ccagggcaa gtactccac gactctctt 
      caccgacga agccgtccg tctaatccg cgagcacaa 
      caccgaaaa ccccatgtt catgtacct cgcccactt 
      ggccccaca ctcgggcaa cgacgacga ccctctgca 
      agcccccga cgaagaaat cgcgaaatt cggacacat 
      cgcagaccc cgaacgtcg aatttacgc cgccatggt 
      ctccatgtt ggacaagag tgtgggcag tgtgatagc 
      cgctttgag ggacaaaca catgctgga aaattcgat 
      tattgtgtt catgtcaga caacggggc aaagcccga 
      tggaatcca cgccaatca cggctcgaa ttatccttt 
      gcggggcaa caagaactc ggcttggga aggggccat 
      gaggtgcgt ggcggcgat ctggagccc ccttatcaa 
      gaaaccgca acgtgtctc gaacagtct gatgcacat 
      ttcggactg gttaccgac tttctacac agctgcagg 
      gctcaataa gaccgaact accgaaaat ggacggggt 
      tgacatgtg ggcctccat ttccgaagg gaaagacag 
      cccccggac tgaactctt acacaacat tgacgaaat 
      ttacaatta tggggcact tagagtcgg gaattggaa 
      gtatctcta cggatccac cacaaatgg taaaagtga 
      tggttggta tgggtcttc aggtcgtga cccccttta 
      cacttacga tgatagcgc cgttttggc ctcacagac 
      cggctcgac acttgccgg tcttacaac ctaccaaca 
      aatcaagga aaaacacca aggtgacac taatttcac 
      acacaaact gctcgacag tgagacgat caagaccct 
      tcggggcgc agccgaagt caagtgtcc gcgagtcaa 
      ttttgagga aatccccga aagtaagaa atgtaacgc 
      agtcgagtc cccttgtct ctttaacat caaggaaga 
      tccctgcga acaaatcaa tttagcggc cgaacgtcc 
      catgattgt cctgaacat ggaaatggc attagcccg 
      attcaaaca aacggctct acccatcag aaacgtccc 
      aagagaccc taatgcgga ccccgccaa gtggaacaa 
      cacttgggt caattggca agactacga agacgtgaa 
      aaagcagaa aatttactt caatacgtt gtcaccgct 
      ggcgattgg cttaatttc cgcagcggt tgttgcctt 
      tgttgtcgt tataattat cctggtgac tatcacagt 
      gaagagttc tttggataa gaaaaaggc cgggaaagc 
      gtttttcga cgatcccat ggagcaaat gatgacgat 
      ggcgccgaa accacaaat tttcgaaga cagggaatt 
      gcaaaatag ggagagtat cagaaatga gtttaggac 
      ggtggaata ggaaacaag cggcttttc gagagaaaa 
      gctcgttct tgtttcgat tgtgatcaa agagctttg 
      ggggtttct tatagaaat gtccacaag caagtaggg 
      ttaaattat tgtaaatat ttattttaa tttagaatg 
      aaaataaat ttattaaaa aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY