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Gene information
General information
TC008433 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10195395..10196977
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008433
    iBeetle screen iB_04489

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva bristle not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva seta not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    head potentially bent - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus distal rounded - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008433 RA
      MTLGQQNKM YNVIQHSPQ QSIIVSGSI TQPTKTVIQ 
      SKRPLAPAP ERTSVLVTN NSDLRCKRK IQFMPYGPQ 
      QQPASVARR NARERNRVK QVNNGFATL RQHIPASVA 
      AAFAPQGPS TGRGASKKL SKVETLRLA VEYIRSLKQ 
      MIEDHENDT TGNSGEGSL QENRYYNNS PDQYQSYQI 
      LLPTPTCSE ASSSPTPSH SSESSFSAA SSYTNTIYH 
      QENFENYEP KKRMNKIER KREASADLD QCLHE
    CDS sequence
    • >TC008433 RA
      atgaccctc ggccagcaa aacaaaatg tacaacgtg 
      atccagcac tcgcctcag caatcaatc atcgtctct 
      ggaagcatc acccaaccc accaaaacc gtcatccag 
      agcaaacga cccctggct ccggccccg gaaaggacg 
      tcggtttta gtcaccaat aactcggat ctgcgatgc 
      aagagaaaa atccagttc atgccctac ggaccccaa 
      cagcaaccg gcgtcggtg gccaggaga aacgccagg 
      gaacgaaac cgagtcaaa caagtaaat aatggattt 
      gcaacgttg agacaacat attcctgca agcgtcgct 
      gcagctttt gccccccaa ggaccatcg acaggacgt 
      ggagcttcg aaaaagctc agtaaagtc gaaacttta 
      agactagca gttgaatac atccggagt ctcaaacaa 
      atgatcgaa gaccatgaa aatgacacg accggaaac 
      tccggtgaa ggctccctc caagaaaac cgatattac 
      aacaacagt ccggaccaa taccaatcc taccaaatt 
      ttactaccc acccccaca tgttcggag gcctcctca 
      tcacccacc ccctcacac agttccgaa agttccttc 
      tcagccgcc tccagctac accaacacg atttaccac 
      caggaaaat ttcgaaaat tacgaaccg aaaaaacga 
      atgaataag atcgagaga aagagagag gccagtgcg 
      gaccttgac cagtgcctt cacgagtga
    mRNA sequence
    • >TC008433 RA
      gcgtcaaag tgccttaaa gtgctcaag ttcttaaaa 
      aatccgtga cttttaacc cacatggca gtcgtccaa 
      acaccgttc gcgatgacc ctcggccag caaaacaaa 
      atgtacaac gtgatccag cactcgcct cagcaatca 
      atcatcgtc tctggaagc atcacccaa cccaccaaa 
      accgtcatc cagagcaaa cgacccctg gctccggcc 
      ccggaaagg acgtcggtt ttagtcacc aataactcg 
      gatctgcga tgcaagaga aaaatccag ttcatgccc 
      tacggaccc caacagcaa ccggcgtcg gtggccagg 
      agaaacgcc agggaacga aaccgagtc aaacaagta 
      aataatgga tttgcaacg ttgagacaa catattcct 
      gcaagcgtc gctgcagct tttgccccc caaggacca 
      tcgacagga cgtggagct tcgaaaaag ctcagtaaa 
      gtcgaaact ttaagacta gcagttgaa tacatccgg 
      agtctcaaa caaatgatc gaagaccat gaaaatgac 
      acgaccgga aactccggt gaaggctcc ctccaagaa 
      aaccgatat tacaacaac agtccggac caataccaa 
      tcctaccaa attttacta cccaccccc acatgttcg 
      gaggcctcc tcatcaccc accccctca cacagttcc 
      gaaagttcc ttctcagcc gcctccagc tacaccaac 
      acgatttac caccaggaa aatttcgaa aattacgaa 
      ccgaaaaaa cgaatgaat aagatcgag agaaagaga 
      gaggccagt gcggacctt gaccagtgc cttcacgag 
      tgatatttt ttgggttct gtttacaaa aaatatatt 
      gttattcta gtcattgtt ttcctcttg tacatactt 
      gtaaatatt gttagttat atacgtagt gctatttta 
      agaaattgg ttgagacat tcctctgct gttattgca 
      atatattgt ataaaaatt tgagaaatt taaaacgat 
      gccttttta tggttcaaa ctcgtactg agagcccca 
      gtaattagt cgttaagtt tttacagtg taagcatgt 
      tccatgact taagctaag ttatttttc gtaattaat 
      ttaagtatt tttgatact actttttta cactgtact 
      gagccaata aacgtgcga tttttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY