Status
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Gene information
General information
TC008430 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10130391..10134746
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008430
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04488

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008430 RA
      MFVETKKMV PPDGGWGWI VVIGVSVVN LCTRSIEPS 
      FGLLFGDLL KELKVETTG AALIMSTLD ALINFSGLF 
      VGPLIRAFS YRKVSLVGS ALCALGLIL TYPANSMAH 
      ILATYSIIN GLGVGLTAS STFVALNNY FVKKRGQAV 
      GLSLAGTAF GMMLMPQAV KLLLEEFSF RGTILILGA 
      FALNAIVGS SLLQPAKWH FVPEIKDEE EGYHQEMET 
      IKETEDEDL DLHEAQTLI NTKGMSELA VKTLEELTK 
      QPGLSRKST FPRNFSTMS FLGSKRRKP SQDSIVSSY 
      SRLDFTGSS LQLHLEQNE SDSSSSSAT HLRRNMSYP 
      GVQLTSHKK ILAEFSKSP LKFQAEKKK KNMWKKIVD 
      FMDFDLLKD PIYLNLVFG LSIFYVAEQ NFKMVTPFF 
      FENIGYKKA DVALFLSVQ AFTDILARL ILPPICDRI 
      NVSKRTLFM TGIFVLGIC RSVLAEQTD WTPIMVWLV 
      ICGFFRGAA LINFTITIS EYSSLEKLP AAFGLHMVG 
      KGLFVVAIG PILGIIRDT TKSYPICLH SQTFLILVC 
      CVAWSIEYA FRFLRTRSV ESDTDTSTT
    CDS sequence
    • >TC008430 RA
      atgttcgtg gaaacaaag aaaatggtc cctccagat 
      ggcggatgg gggtggatc gtcgtcata ggagtcagt 
      gtcgttaat ttatgtact cgttcaatc gagccttct 
      ttcggttta ctcttcggc gacttgctg aaagaactg 
      aaggttgaa acgaccgga gccgctctt ataatgagc 
      actttggac gcattgatc aacttttca ggacttttc 
      gtaggacct ctaatccgt gccttctca tatcgtaaa 
      gtttccctc gttggatca gctttatgc gccctcggg 
      ctgatcttg acctatccc gccaacagt atggcgcac 
      atccttgct acgtacagt attatcaac ggtttagga 
      gtaggttta accgcatct tcgactttc gtcgcactg 
      aacaactat ttcgtgaaa aagcgaggt caagccgtg 
      ggtctttcg ctggcaggg acggctttt ggcatgatg 
      ttgatgcca caagccgtc aagttgctc ttagaagag 
      ttcagtttt cgcgggacg attttaatt ttgggagca 
      tttgctctg aatgcgatt gttgggtcc tcgttgcta 
      caaccggcg aagtggcat ttcgtgccc gaaatcaag 
      gacgaggaa gaaggttat catcaggaa atggagacg 
      atcaaagaa acagaagac gaagatctc gacttgcac 
      gaagcacaa actttgatc aatacgaaa ggaatgtcc 
      gaattggct gtgaaaacg ttagaagaa ttgacaaaa 
      cagccaggc ttgtcgcga aagtcgact tttcctcga 
      aatttttcg acgatgagt tttctcggg agcaaaaga 
      cggaaaccg tcgcaggat tcgatcgtt tcaagttac 
      tcgcgattg gattttacc ggttccagt cttcaatta 
      catctagag caaaacgaa tccgattcg agttcgagt 
      tccgccact catcttcgc cgcaacatg agttatcca 
      ggcgttcag ctcacttcg cacaagaaa atcttagcc 
      gaattctcc aaatcgccg ctgaagttt caagccgag 
      aagaagaag aagaacatg tggaaaaaa atcgtcgac 
      tttatggat ttcgatttg ttaaaagat cccatctac 
      ttgaacctg gtttttggt ttgtcgatt ttctacgtg 
      gctgagcag aattttaaa atggtgaca ccgttcttc 
      ttcgaaaat atcggctac aaaaaagcg gacgtagcg 
      ctgttttta tccgtccaa gcgttcact gatatcctg 
      gcaaggctg attctgccg ccgatctgc gacaggatc 
      aacgtgtcg aaaaggaca cttttcatg accgggatt 
      tttgttttg ggtatttgt cgatcagtc ctcgctgaa 
      cagaccgat tggacccct attatggtt tggttggtc 
      atttgtggg ttcttcagg ggggctgcc ctcattaat 
      ttcacaatc acgatatcg gaatattcc tctttggag 
      aaactacca gcggctttc gggctacac atggtgggg 
      aaggggctg tttgtcgtg gcaattggc cccatttta 
      ggtatcata agggacacg accaagagt tacccgatc 
      tgtcttcat tcgcaaact tttcttatt cttgtctgc 
      tgcgtggct tggagcatc gaatatgct ttcaggttt 
      ctcagaact aggagtgtg gagtcggac acggacacc 
      tcgaccaca tga
    mRNA sequence
    • >TC008430 RA
      cgtgattat ttatcttat ccatttacg atttgattg 
      attttttta ttgtcgttt ttgcgataa aacattttg 
      agtacttct gcatttgtt tcaataaaa gaaaccgcg 
      atggttttg tggtagtag tgatggttg gactttaaa 
      atgttctat tgtgttcct agttcttag tttaacaca 
      ggtttgtgg tgttccttt ttatttgag tagtgttcc 
      tctctccat cgatccgga aactccggg caaattcac 
      tcacctcgc ccaacagat taacggttc gtcatagga 
      attttccta ccgcaagcg cccttgatg tgtaccctt 
      atttgcacc cctaccctt aaccctcgc cgtgttcca 
      gattcggat caatgatcg caaggtcgt tcgaatttt 
      tcgcgtttt ttccccgtg taccgtaaa tttataaac 
      agagctaat tgaggcgaa tttttgaaa atttgctaa 
      aacacgtgt tctgcgcgt gaacagtag aacgtcacg 
      tgacgtctt tgcatggga tcgatgatg gaattctgt 
      tctgtccgt cgacgaccg ggccacaaa ctgaaagtc 
      agtcgagtt attagttgg taacaacta cgaaaaaag 
      tgccctcac gtgagatga ggccagaac catgttcgt 
      ggaaacaaa gaaaatggt ccctccaga tggcggatg 
      ggggtggat cgtcgtcat aggagtcag tgtcgttaa 
      tttatgtac tcgttcaat cgagccttc tttcggttt 
      actcttcgg cgacttgct gaaagaact gaaggttga 
      aacgaccgg agccgctct tataatgag cactttgga 
      cgcattgat caacttttc aggactttt cgtaggacc 
      tctaatccg tgccttctc atatcgtaa agtttccct 
      cgttggatc agctttatg cgccctcgg gctgatctt 
      gacctatcc cgccaacag tatggcgca catccttgc 
      tacgtacag tattatcaa cggtttagg agtaggttt 
      aaccgcatc ttcgacttt cgtcgcact gaacaacta 
      tttcgtgaa aaagcgagg tcaagccgt gggtctttc 
      gctggcagg gacggcttt tggcatgat gttgatgcc 
      acaagccgt caagttgct cttagaaga gttcagttt 
      tcgcgggac gattttaat tttgggagc atttgctct 
      gaatgcgat tgttgggtc ctcgttgct acaaccggc 
      gaagtggca tttcgtgcc cgaaatcaa ggacgagga 
      agaaggtta tcatcagga aatggagac gatcaaaga 
      aacagaaga cgaagatct cgacttgca cgaagcaca 
      aactttgat caatacgaa aggaatgtc cgaattggc 
      tgtgaaaac gttagaaga attgacaaa acagccagg 
      cttgtcgcg aaagtcgac ttttcctcg aaatttttc 
      gacgatgag ttttctcgg gagcaaaag acggaaacc 
      gtcgcagga ttcgatcgt ttcaagtta ctcgcgatt 
      ggattttac cggttccag tcttcaatt acatctaga 
      gcaaaacga atccgattc gagttcgag ttccgccac 
      tcatcttcg ccgcaacat gagttatcc aggcgttca 
      gctcacttc gcacaagaa aatcttagc cgaattctc 
      caaatcgcc gctgaagtt tcaagccga gaagaagaa 
      gaagaacat gtggaaaaa aatcgtcga ctttatgga 
      tttcgattt gttaaaaga tcccatcta cttgaacct 
      ggtttttgg tttgtcgat tttctacgt ggctgagca 
      gaattttaa aatggtgac accgttctt cttcgaaaa 
      tatcggcta caaaaaagc ggacgtagc gctgttttt 
      atccgtcca agcgttcac tgatatcct ggcaaggct 
      gattctgcc gccgatctg cgacaggat caacgtgtc 
      gaaaaggac acttttcat gaccgggat ttttgtttt 
      gggtatttg tcgatcagt cctcgctga acagaccga 
      ttggacccc tattatggt ttggttggt catttgtgg 
      gttcttcag gggggctgc cctcattaa tttcacaat 
      cacgatatc ggaatattc ctctttgga gaaactacc 
      agcggcttt cgggctaca catggtggg gaaggggct 
      gtttgtcgt ggcaattgg ccccatttt aggtatcat 
      aagggacac gaccaagag ttacccgat ctgtcttca 
      ttcgcaaac ttttcttat tcttgtctg ctgcgtggc 
      ttggagcat cgaatatgc tttcaggtt tctcagaac 
      taggagtgt ggagtcgga cacggacac ctcgaccac 
      atgacaaac ggatgttga ctgctaagt cacattttt 
      gagggacag aaaaaattc aggaacggt ccgtttggt 
      tttattgtt ttgtcggtt tcttcggtt attttattt 
      ataagttcg aacattttc tatgatatt ctacgtgtg 
      atatgatta ggtttaatg agactgaat ctgtgagta 
      tagtgttcc atgaagata cttaatcta cttcgggta 
      gaccaacat tgtacatag ttttatgat ttaaagtag 
      caataatcc aagatgtat taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY