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Gene information
General information
TC032938
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008427 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10099631..10106668
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032938
    iBeetle screen iB_04487

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%
    procephalic appendages mostlyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    gnathal appendages mostly remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032938 RA
      MAREEVQVP LRDKPLKQL NRIAKRPKL DVEFQDLKY 
SIRDSNAKG GWRTILNSV NGKFRCGEF TAILGPSGA 
GKSSLLNIL AGCVTGGVK GSIKINDRP RDMKIFNKL 
SSYIMQEDC IQPRLTVKE AMIYAACLK LGAHIEYRD 
KLAVIHEVI DLLGLEKCV DTLSEHLSG GQRKRLSVA 
LELVNNPPV IFLDEPTTG LDNFAINQC IHLLKKISQ 
LDRTVVCTI HQPPASIFQ YFDQVYIVA NGYCVYNGA 
PNQLVPFFN VVGHSCPSN NTPADYMIE LIHSQPTLI 
QSFQAAIQN GKINMKERY QSDKPAKEY TNDGVYNDT 
TEVVIQKSD IDFATSFNV QFSILLSRM FLQMRRNRL 
GLYIQFFHH LLSGLIVGG IFYGIGNDA AQTIAIFKY 
CVCINVFFM YTHVMMPVL LFPLEVSLM KREYFNRWY 
GLKAYYAAM TVVTLPPLI ILGMMFSTI VYFMSHQPL 
ETDRFLGFT LTGLAVALT SQGLGYCIG SIFSITNGS 
VVGPSVLAP LLALAVYGM GYRASIEPF YKVLMTLTY 
IRNGVVGIC NSLFYERPP LTCGEEEIY CHYARPDIL 
MGDMAIPLM HYRYQLGII CIFMIVFRI VGYFSLKCR 
LNNDLATKI MFYAKKIIK HKY
    CDS sequence
    • >TC032938 RA
      atggccaga gaggaggta caagtacct ctgagagac 
aaaccgttg aaacagttg aaccgaatt gcgaaaaga 
ccaaaattg gatgtcgaa tttcaagat ttgaagtat 
tcgattagg gatagtaac gccaagggg ggatggagg 
actattttg aattctgta aacggaaag ttccgttgt 
ggcgaattc acagcgata ctgggacca agcggtgcc 
ggaaaaagc tctctactg aatatcctt gcaggttgt 
gtaaccgga ggtgtcaaa ggttcgatc aaaatcaac 
gatcgtcct cgagatatg aaaattttc aacaaattg 
tcttcatac atcatgcaa gaggattgc atccaaccg 
agattaaca gtaaaagag gcaatgata tacgcagca 
tgtctcaaa ttaggggcc catatcgaa taccgtgat 
aaactggct gtgattcac gaagtgatc gatctttta 
gggctggaa aagtgtgtg gatactcta agtgagcat 
ctgtcggga ggtcaacgc aaaaggtta agtgttgct 
cttgaacta gttaataat cctccggtg attttcctg 
gacgagccg acgactggt ttggataat tttgccata 
aaccagtgt atccactta ttgaaaaaa atcagccag 
ttggatcga actgtcgtt tgcacgatt catcaacct 
ccggcttcc atctttcaa tatttcgat caggtatac 
atcgttgct aatggatat tgtgtttac aacggtgcc 
ccgaaccaa ttggttcca tttttcaac gtcgttggc 
cactcttgc ccttcaaac aacactccg gccgattac 
atgatcgaa ttaattcac tcgcaaccg accttgatt 
caaagcttc caggctgcg atacaaaac ggaaagatc 
aacatgaag gagcgctac cagagcgac aaaccggcg 
aaagaatac acgaacgat ggtgtttat aatgacacc 
accgaagtc gtaattcaa aaaagcgac attgatttt 
gcgacttcg ttcaacgtc cagttttcg attctcttg 
tctcgcatg tttttacaa atgagacgc aatcgtcta 
ggactctac atccagttc ttccatcat ttgctgtcc 
ggtttaata gtcggtggt attttctac ggaattgga 
aacgacgcc gcacaaacg atcgcaatt tttaaatat 
tgcgtctgt attaatgtg ttttttatg tacacacat 
gttatgatg ccggtgctt ttgtttcct ttggaagtc 
agtttgatg aaacgagaa tattttaat agatggtac 
ggactcaag gcttattac gcggccatg acagtcgtc 
acgttacca ccactgatt attctgggg atgatgttt 
tcaacaatt gtctatttc atgtcacac caacctctg 
gaaactgat cggtttctc gggtttact ttaacaggg 
cttgcagtg gcgctcact tcgcaaggt ttaggttat 
tgcatcggt tccattttt agtataact aatggttcg 
gtggtcgga ccttcagtc ctggcacca cttctagcc 
cttgctgtt tacggtatg ggttatagg gcttccatc 
gaacccttc tacaaggtg ttaatgact ttgacttac 
attcgaaac ggtgttgtt ggcatttgt aacagtttg 
ttttacgag agaccgcct ctaacttgc ggtgaggag 
gaaatttat tgtcattac gcgcgtcct gatatactt 
atgggtgac atggcaata cctctgatg cactatcgg 
taccaacta ggcattatt tgcattttc atgattgtt 
tttcgaatc gttggttac ttttcgtta aagtgtcga 
ctgaacaat gatctagcc acgaaaata atgttctac 
gcgaaaaaa atcatcaaa cataaatat taa
    mRNA sequence
    • >TC032938 RA
      cccttatta ccacacgtt tgttgctgt ttttcttta 
ttatctgca aaaaactgg tgttctagt gcacttttg 
atctcaatt ctattggaa aattttgtt aatagctag 
ctttgaact cttttaatt ttgcgcgaa ttatggcca 
attttgagt ctaaatttt gtaattggt ctaggtaga 
accagttcg cttgcaaac tgcatagag aagcgtaga 
agagtcaag gcctacatt tgcaagaag tgcaagttt 
acggtcatt gtcacaccg tttccgtaa atcgttgaa 
attcgatat aaatgctca taataagtg ttttcgaag 
tcgggagtc gaatcgtga aaggttcac tttacattg 
ttcagtgaa agcgtacag gtttcctcg ctgatattt 
tagtctagc agggacgtt tcgcgattg ctgtacgct 
ttccgttgt tgttggtgt tcagtaatg gacgtgaaa 
atggccaga gaggaggta caagtacct ctgagagac 
aaaccgttg aaacagttg aaccgaatt gcgaaaaga 
ccaaaattg gatgtcgaa tttcaagat ttgaagtat 
tcgattagg gatagtaac gccaagggg ggatggagg 
actattttg aattctgta aacggaaag ttccgttgt 
ggcgaattc acagcgata ctgggacca agcggtgcc 
ggaaaaagc tctctactg aatatcctt gcaggttgt 
gtaaccgga ggtgtcaaa ggttcgatc aaaatcaac 
gatcgtcct cgagatatg aaaattttc aacaaattg 
tcttcatac atcatgcaa gaggattgc atccaaccg 
agattaaca gtaaaagag gcaatgata tacgcagca 
tgtctcaaa ttaggggcc catatcgaa taccgtgat 
aaactggct gtgattcac gaagtgatc gatctttta 
gggctggaa aagtgtgtg gatactcta agtgagcat 
ctgtcggga ggtcaacgc aaaaggtta agtgttgct 
cttgaacta gttaataat cctccggtg attttcctg 
gacgagccg acgactggt ttggataat tttgccata 
aaccagtgt atccactta ttgaaaaaa atcagccag 
ttggatcga actgtcgtt tgcacgatt catcaacct 
ccggcttcc atctttcaa tatttcgat caggtatac 
atcgttgct aatggatat tgtgtttac aacggtgcc 
ccgaaccaa ttggttcca tttttcaac gtcgttggc 
cactcttgc ccttcaaac aacactccg gccgattac 
atgatcgaa ttaattcac tcgcaaccg accttgatt 
caaagcttc caggctgcg atacaaaac ggaaagatc 
aacatgaag gagcgctac cagagcgac aaaccggcg 
aaagaatac acgaacgat ggtgtttat aatgacacc 
accgaagtc gtaattcaa aaaagcgac attgatttt 
gcgacttcg ttcaacgtc cagttttcg attctcttg 
tctcgcatg tttttacaa atgagacgc aatcgtcta 
ggactctac atccagttc ttccatcat ttgctgtcc 
ggtttaata gtcggtggt attttctac ggaattgga 
aacgacgcc gcacaaacg atcgcaatt tttaaatat 
tgcgtctgt attaatgtg ttttttatg tacacacat 
gttatgatg ccggtgctt ttgtttcct ttggaagtc 
agtttgatg aaacgagaa tattttaat agatggtac 
ggactcaag gcttattac gcggccatg acagtcgtc 
acgttacca ccactgatt attctgggg atgatgttt 
tcaacaatt gtctatttc atgtcacac caacctctg 
gaaactgat cggtttctc gggtttact ttaacaggg 
cttgcagtg gcgctcact tcgcaaggt ttaggttat 
tgcatcggt tccattttt agtataact aatggttcg 
gtggtcgga ccttcagtc ctggcacca cttctagcc 
cttgctgtt tacggtatg ggttatagg gcttccatc 
gaacccttc tacaaggtg ttaatgact ttgacttac 
attcgaaac ggtgttgtt ggcatttgt aacagtttg 
ttttacgag agaccgcct ctaacttgc ggtgaggag 
gaaatttat tgtcattac gcgcgtcct gatatactt 
atgggtgac atggcaata cctctgatg cactatcgg 
taccaacta ggcattatt tgcattttc atgattgtt 
tttcgaatc gttggttac ttttcgtta aagtgtcga 
ctgaacaat gatctagcc acgaaaata atgttctac 
gcgaaaaaa atcatcaaa cataaatat taatcgttg 
gtattttgt tagttagta cctgcttga atgtgataa 
ttagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY