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Gene information
General information
TC008423 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10049181..10063318
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008423
    iBeetle screen iB_04486

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 9 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008423 RA
      MYGLILENM SEYIKQTYG EEKWEEIRR AAAVDQPSF 
      STHQVYPEA LIPRLSKKA VQILKVSEK DFFEQMGVF 
      FVSFVSQYG YDRVLSVLG RHMRDFLNG LDNLHEYLK 
      FSYPRMRAP SFICENETK QGLTLHYRS KRRGFVYYT 
      MGQIKEVAR HFYNKEMKI ELVREELLF DMVHVTFQL 
      TFDNRAFTL ASLAMTREE KHLPISASV LFEIFPFCI 
      VFGSDMIVR SIGNSLMVI LPDLIGKKI TNWFDLVRP 
      LTAFKFQTI LNRTNNIFE LVTVEPILH EKPPERGRQ 
      EMILSDELD LEEDRSLRL KGQMIYMDN WKMMMYLGT 
      PVMPDLNSL INSGLYIND LSMHDFSRD LMLAGTQQS 
      VELKLALDQ EQQKSKKLE ESMRKLDEE MRRTDELLY 
      QMIPKQVAD RLRKGENPI DTCEMFDSV SILFSDVVT 
      FTEICSRIT PMEVVSMLN GMYSIFDTL TERNRVYKV 
      ETIGDAYMV VSGAPEKEG NHAERVCDM ALDMVDAIT 
      DLKDPSTGQ HLRIRVGVH SGAVVAGIV GLKMPRYCL 
      FGDSVNTAS RMESTSEAM MIHISEYTR QLLPPAYTV 
      LERGEIQVK GKGHMKTYW LKGKESRTP IDRREKAPL 
      HQEIDPLDN REFKHPNVH AEDRRSVVY SPITFQDVA 
      RRSIASSPV KYSQSTREL RSNSAGAVY GMRDPADIF 
      GAMVLDNSP IKEDTDRPL KTFTQEVSL VAQGSPLTS 
      PPSIFLSNH TEKSPKAQF DKKDKDNNP YEFGRSMYN 
      YAKKFEGRK NEKGAHSEM PVSKSDGDL QGKKTLVNL 
      SNSHLNKSN HHHQHQCCS GFGAGNRHR FQSNACVIN
    CDS sequence
    • >TC008423 RA
      atgtacggc ctaatcctg gaaaatatg tccgagtac 
      ataaagcaa acctacggc gaggaaaaa tgggaagag 
      ataaggcga gcagcggcc gtcgatcaa cccagtttc 
      agtacccac caggtttat cctgaagct ctgatccct 
      cgtctgtcg aaaaaagcc gttcagata ttaaaagtg 
      agcgagaag gattttttc gagcaaatg ggtgtgttt 
      ttcgtcagt ttcgtcagt cagtacgga tacgatcga 
      gtgctttcg gtcctcggc cgccacatg agggacttc 
      ctcaacggg ttggataat ctccacgag tatttaaaa 
      ttttcgtat cctcggatg cgggcgccg agcttcatt 
      tgcgagaac gagacgaag caaggactg accttacat 
      taccggagc aaaagacgg gggtttgtt tattacacg 
      atggggcaa atcaaggaa gtagctaga catttctac 
      aataaagaa atgaaaatc gagctggtg cgggaagag 
      ctgcttttc gacatggtc catgtcact ttccagtta 
      acgttcgat aaccgggct ttcactctc gcatcgctg 
      gcgatgaca agagaagaa aaacactta ccgattagt 
      gcgtctgtt ttattcgag attttcccg ttttgtata 
      gttttcggt tcggatatg attgtccgc agcataggc 
      aactcgctc atggtgatt ttgcccgac ttaattggg 
      aaaaaaata acgaattgg ttcgattta gttcggcct 
      ttgacagcg ttcaagttt caaacgatt ttaaatcgt 
      acaaataat atttttgaa ctagtgacg gtcgaaccg 
      atccttcat gagaaaccg cccgagaga gggaggcaa 
      gaaatgata cttagcgac gagctggat ttggaggag 
      gacaggagt ttgagactt aaagggcaa atgatttac 
      atggacaat tggaagatg atgatgtat ctaggaacc 
      ccagtgatg cctgatctg aattcgcta atcaattcc 
      gggctttat attaatgat ctctccatg cacgatttt 
      agcagggac ttgatgttg gctggaacc caacagtca 
      gtcgaactg aaattagct ctagaccag gaacaacag 
      aaaagtaaa aaattggag gaatcgatg cggaaactg 
      gacgaggaa atgagacga actgacgag ttgctttac 
      caaatgatt ccaaaacaa gtagccgat agattacgg 
      aaaggcgaa aatccgatc gatacatgc gagatgttc 
      gacagcgtt tcaatcctc ttctccgat gtcgtcaca 
      ttcacggaa atttgcagc aggattacg ccgatggaa 
      gttgtctca atgctgaat ggcatgtat tcaatattc 
      gatactctg actgagaga aaccgggtg tacaaagtg 
      gagacaatt ggtgatgct tacatggtg gtttctggg 
      gccccggaa aaggagggc aaccacgcg gaacgcgtc 
      tgcgacatg gccttggac atggtggac gccatcacc 
      gacctgaaa gacccttcc acgggtcag catttacgg 
      atccgggtt ggggtccat tcgggcgcg gttgttgca 
      gggatagta gggttgaaa atgccccga tattgcctg 
      tttggcgat agtgtaaat accgcctct cggatggaa 
      tcgacaagt gaggcgatg atgatccac atttccgaa 
      tatacgcgg caactgcta ccccctgcg tacaccgta 
      ctggaacga ggggagatc caagtgaag ggaaaaggt 
      catatgaaa acgtattgg ttgaaggga aaagagtca 
      aggacgccg atagatcgg cgggagaaa gcaccactg 
      caccaagag atcgatcct ttggataat agagagttt 
      aaacaccct aatgttcac gcggaggat agaaggagt 
      gtcgtttat tctccgatc acatttcag gatgtggca 
      cggaggagt attgcgagt tcgccggtt aaatattcg 
      caaagtacg agagagctg cgttcgaat tctgctggg 
      gctgtgtac gggatgcgc gacccggct gatattttc 
      ggggccatg gtcctcgac aattctcca attaaggaa 
      gacacggac aggccctta aagactttc acccaggaa 
      gtaagttta gtggctcag ggatcaccc ttgacatct 
      ccaccctca attttccta tcaaaccat acagagaag 
      tccccgaag gctcaattt gataaaaaa gataaagac 
      aataacccg tacgaattc gggcgatcg atgtacaac 
      tacgcgaag aaattcgag gggaggaag aacgaaaag 
      ggggcgcat tcggagatg cccgtctcg aagagcgac 
      ggcgacctc caaggcaag aaaactcta gttaattta 
      tcgaacagt catttgaac aaatccaac caccatcac 
      cagcaccag tgttgctcc gggttcggg gccgggaat 
      agacacagg ttccaaagc aacgcttgt gttatcaac 
      taa
    mRNA sequence
    • >TC008423 RA
      atacgcagg atgcaagga ctgaaattt ttttcagga 
      tacgagtcg cgatctcac tggcgttcg ctttctact 
      ttgtgtgcg acgagtttc agtgactgt gatattaat 
      ttttaagta gcggatttt gagtttttg caggaattc 
      agccaagat caatgcaac atgtacggc ctaatcctg 
      gaaaatatg tccgagtac ataaagcaa acctacggc 
      gaggaaaaa tgggaagag ataaggcga gcagcggcc 
      gtcgatcaa cccagtttc agtacccac caggtttat 
      cctgaagct ctgatccct cgtctgtcg aaaaaagcc 
      gttcagata ttaaaagtg agcgagaag gattttttc 
      gagcaaatg ggtgtgttt ttcgtcagt ttcgtcagt 
      cagtacgga tacgatcga gtgctttcg gtcctcggc 
      cgccacatg agggacttc ctcaacggg ttggataat 
      ctccacgag tatttaaaa ttttcgtat cctcggatg 
      cgggcgccg agcttcatt tgcgagaac gagacgaag 
      caaggactg accttacat taccggagc aaaagacgg 
      gggtttgtt tattacacg atggggcaa atcaaggaa 
      gtagctaga catttctac aataaagaa atgaaaatc 
      gagctggtg cgggaagag ctgcttttc gacatggtc 
      catgtcact ttccagtta acgttcgat aaccgggct 
      ttcactctc gcatcgctg gcgatgaca agagaagaa 
      aaacactta ccgattagt gcgtctgtt ttattcgag 
      attttcccg ttttgtata gttttcggt tcggatatg 
      attgtccgc agcataggc aactcgctc atggtgatt 
      ttgcccgac ttaattggg aaaaaaata acgaattgg 
      ttcgattta gttcggcct ttgacagcg ttcaagttt 
      caaacgatt ttaaatcgt acaaataat atttttgaa 
      ctagtgacg gtcgaaccg atccttcat gagaaaccg 
      cccgagaga gggaggcaa gaaatgata cttagcgac 
      gagctggat ttggaggag gacaggagt ttgagactt 
      aaagggcaa atgatttac atggacaat tggaagatg 
      atgatgtat ctaggaacc ccagtgatg cctgatctg 
      aattcgcta atcaattcc gggctttat attaatgat 
      ctctccatg cacgatttt agcagggac ttgatgttg 
      gctggaacc caacagtca gtcgaactg aaattagct 
      ctagaccag gaacaacag aaaagtaaa aaattggag 
      gaatcgatg cggaaactg gacgaggaa atgagacga 
      actgacgag ttgctttac caaatgatt ccaaaacaa 
      gtagccgat agattacgg aaaggcgaa aatccgatc 
      gatacatgc gagatgttc gacagcgtt tcaatcctc 
      ttctccgat gtcgtcaca ttcacggaa atttgcagc 
      aggattacg ccgatggaa gttgtctca atgctgaat 
      ggcatgtat tcaatattc gatactctg actgagaga 
      aaccgggtg tacaaagtg gagacaatt ggtgatgct 
      tacatggtg gtttctggg gccccggaa aaggagggc 
      aaccacgcg gaacgcgtc tgcgacatg gccttggac 
      atggtggac gccatcacc gacctgaaa gacccttcc 
      acgggtcag catttacgg atccgggtt ggggtccat 
      tcgggcgcg gttgttgca gggatagta gggttgaaa 
      atgccccga tattgcctg tttggcgat agtgtaaat 
      accgcctct cggatggaa tcgacaagt gaggcgatg 
      atgatccac atttccgaa tatacgcgg caactgcta 
      ccccctgcg tacaccgta ctggaacga ggggagatc 
      caagtgaag ggaaaaggt catatgaaa acgtattgg 
      ttgaaggga aaagagtca aggacgccg atagatcgg 
      cgggagaaa gcaccactg caccaagag atcgatcct 
      ttggataat agagagttt aaacaccct aatgttcac 
      gcggaggat agaaggagt gtcgtttat tctccgatc 
      acatttcag gatgtggca cggaggagt attgcgagt 
      tcgccggtt aaatattcg caaagtacg agagagctg 
      cgttcgaat tctgctggg gctgtgtac gggatgcgc 
      gacccggct gatattttc ggggccatg gtcctcgac 
      aattctcca attaaggaa gacacggac aggccctta 
      aagactttc acccaggaa gtaagttta gtggctcag 
      ggatcaccc ttgacatct ccaccctca attttccta 
      tcaaaccat acagagaag tccccgaag gctcaattt 
      gataaaaaa gataaagac aataacccg tacgaattc 
      gggcgatcg atgtacaac tacgcgaag aaattcgag 
      gggaggaag aacgaaaag ggggcgcat tcggagatg 
      cccgtctcg aagagcgac ggcgacctc caaggcaag 
      aaaactcta gttaattta tcgaacagt catttgaac 
      aaatccaac caccatcac cagcaccag tgttgctcc 
      gggttcggg gccgggaat agacacagg ttccaaagc 
      aacgcttgt gttatcaac taacgacaa tatctcgat 
      gtaaatgac cagatctct tcatgtttc agtagacta 
      ggcttcaaa attcgatgt gataggaca caaaatact 
      catcttaat actgtattt tttaaaaaa atatttgta 
      cctctcata attcgtagg tttatgtac atatcgtca 
      ataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY