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Gene information
General information
TC008419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10034631..10036279
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008419
    iBeetle screen iB_04484

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head capsule not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    mandible not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: maxilla and labium seem to be less affected than the other head appendages.
    prothorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008419 RA
      MKDIYTVLL ALPLILHNF EITCAPVED DSLILVHVI 
      FRHGNRTPS LEEMYPKDP YRNEKYFPF GLGQLTNVG 
      KKREFMIGK ALRNRYNKF LGPYYYPEI VEARSTDYN 
      RTKMSLELA LAGLFPPRG EEVWNYWLN WQPVPYNYV 
      PQANDNVLL GTLCPNFVK KTKEYLQSG REQTELAKY 
      REVLDYISE NAGFNVTSF LDVYSLYFG LTTEAEWGF 
      ELPEWTQKV YPEPITQLA INEYYTQTA TTELMQMSA 
      GYFLQKVIQ DSYSKINNT NSDRKIYMY AAHENNIAD 
      LLILLGVFE PPHIPNYGA YVLLELHSV NNKYGIRIF 
      YENYQETRP QLLKVPNCD SFCKIDQFA SLYEEYMPD 
      PDLCYSV
    CDS sequence
    • >TC008419 RA
      atgaaagac atttacacg gtcttgctg gcactgcct 
      ttaattttg cacaacttt gaaatcact tgtgctcca 
      gttgaagac gacagccta attctggtc catgtcatt 
      ttcaggcat gggaatcga accccatcc cttgaagaa 
      atgtacccc aaagacccc taccgaaac gaaaagtac 
      ttcccgttt gggttagga caactcact aatgtcggc 
      aaaaaacgc gaattcatg attgggaaa gccctcagg 
      aaccgatac aataaattt ttagggccg tactactat 
      cccgaaatt gtcgaagcg cgaagtacc gactacaac 
      aggactaag atgtcgctg gagctcgcc cttgcaggg 
      ctgtttccg ccaaggggc gaagaagtt tggaattat 
      tggttaaac tggcagccg gttccctac aattacgtc 
      cctcaagcc aacgataac gttttgctc gggacattg 
      tgcccaaac tttgtgaaa aaaaccaag gagtacctg 
      caatctggt cgcgaacag accgaactt gccaaatat 
      cgagaagtt ttggactac atttcggaa aacgcagga 
      ttcaatgtt acaagtttt ctggacgtg tacagttta 
      tatttcggg ttgacaacc gaggcagag tggggtttc 
      gaattaccc gaatggacc caaaaagtt taccctgaa 
      ccgatcaca caattggcc ataaatgag tactacact 
      cagaccgca accaccgaa ctgatgcaa atgtcggct 
      ggctatttc ctgcaaaaa gtaatccaa gattcctat 
      tccaaaatc aacaacacc aactccgac cggaaaatc 
      tacatgtac gcggcccac gagaacaat attgcggac 
      cttttgatt ttgttgggg gtgtttgag cctccccac 
      ataccgaat tatggggcc tacgtgctt ttggagctt 
      cacagtgtg aataacaag tacgggatt aggatattt 
      tacgaaaat taccaagag actaggcca caactcttg 
      aaggttcca aattgcgac agtttttgc aaaatcgac 
      cagtttgcg agtttgtac gaagaatac atgcctgat 
      cctgattta tgttacagt gtgtaa
    mRNA sequence
    • >TC008419 RA
      ctttattca ctcgcttgg cgaaattca aaacagtga 
      actacaaat tgcacattc gaaaaagtc attcaaggt 
      atcaggcac atagtaatt aatatttgc gttaattgg 
      cttcagttc ctttgcaaa atgaaagac atttacacg 
      gtcttgctg gcactgcct ttaattttg cacaacttt 
      gaaatcact tgtgctcca gttgaagac gacagccta 
      attctggtc catgtcatt ttcaggcat gggaatcga 
      accccatcc cttgaagaa atgtacccc aaagacccc 
      taccgaaac gaaaagtac ttcccgttt gggttagga 
      caactcact aatgtcggc aaaaaacgc gaattcatg 
      attgggaaa gccctcagg aaccgatac aataaattt 
      ttagggccg tactactat cccgaaatt gtcgaagcg 
      cgaagtacc gactacaac aggactaag atgtcgctg 
      gagctcgcc cttgcaggg ctgtttccg ccaaggggc 
      gaagaagtt tggaattat tggttaaac tggcagccg 
      gttccctac aattacgtc cctcaagcc aacgataac 
      gttttgctc gggacattg tgcccaaac tttgtgaaa 
      aaaaccaag gagtacctg caatctggt cgcgaacag 
      accgaactt gccaaatat cgagaagtt ttggactac 
      atttcggaa aacgcagga ttcaatgtt acaagtttt 
      ctggacgtg tacagttta tatttcggg ttgacaacc 
      gaggcagag tggggtttc gaattaccc gaatggacc 
      caaaaagtt taccctgaa ccgatcaca caattggcc 
      ataaatgag tactacact cagaccgca accaccgaa 
      ctgatgcaa atgtcggct ggctatttc ctgcaaaaa 
      gtaatccaa gattcctat tccaaaatc aacaacacc 
      aactccgac cggaaaatc tacatgtac gcggcccac 
      gagaacaat attgcggac cttttgatt ttgttgggg 
      gtgtttgag cctccccac ataccgaat tatggggcc 
      tacgtgctt ttggagctt cacagtgtg aataacaag 
      tacgggatt aggatattt tacgaaaat taccaagag 
      actaggcca caactcttg aaggttcca aattgcgac 
      agtttttgc aaaatcgac cagtttgcg agtttgtac 
      gaagaatac atgcctgat cctgattta tgttacagt 
      gtgtaaaaa taaataaat ttaccaacc tgcccactt 
      gttttgtga gtgtcgata aggaaggat ttgctccgt 
      ttgcttaat tttgcggtt gagacgaaa gtaaacaaa 
      tccagacaa gatgaacat catgcttca agataaaaa 
      acctaaagc taacacaga caattaata aatcgtaaa 
      ttataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY