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Gene information
General information
TC008417 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10007151..10030270
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008417
    iBeetle screen iB_04483

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen musculature partially rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: because nearly all embryos are lying lateral, it is not possible to say what exactly is wrong with the thoracic muscles, only that there is soething wrong. leg muscles seem to be not affected (at least they are not rounded or missing)
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 6


    elytra surface irregular - Number of animals showing the phenotype: 6


    elytra colour irregular - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008417 RA
      MWIADLRIV LGFCICTAL AYQGSKQDY IKWRERFLP 
      KRPTTTESA VTSANDIHY ENLKLRHHS RFPNLDRVL 
      VKIDNLQKK NDVQPVHNR IRHQNVYKT TTTTTTTTD 
      EGLFGDDYS DPEYEDDNN DELNDYDEW DDENFDDYD 
      DETFDSDDP FADEWQNEE ATEPPAPVS SFTKYKWDN 
      YGTRSRVEE SRRQQLHLK PHNEGRAIN AALEHYAKV 
      RQEGQCRKP LPKVISVQS EHPDPSKTY TPHCTILHR 
      CADDTGCCA NHTTRCGPK TQVLVHLYF YAKTLGVPG 
      TKVEKLTFY NHTECACIE KSKGPTTLP DENINLTHG 
      LSLKQGSEP ENMRNCKCP EEFTPKFRY DSRCSCDCE 
      EYNQDCIRM KKGKEYFSL TDRRCIIEN QCGIPVCEY 
      GVYMRHMGR CQRKQEKFD SFSKMTVK
    CDS sequence
    • >TC008417 RA
      atgtggatc gccgatttg agaatagtg ctgggcttc 
      tgcatctgt acggctttg gcctaccaa ggatccaag 
      caggattac atcaaatgg cgggagcgg ttccttccg 
      aagaggccg acgacgacg gagagtgca gtgactagt 
      gcaaacgat atccactac gaaaatctg aaattgagg 
      caccattcg cgcttcccg aatttggac agagttctg 
      gtcaagata gacaatttg cagaagaag aacgacgtg 
      cagccggtt cataatagg attaggcac cagaacgtg 
      tacaagacc accaccacg accaccacc acgactgac 
      gaaggcctt ttcggcgac gattattcc gatccggag 
      tacgaagat gacaacaac gatgagctc aatgactac 
      gacgagtgg gacgacgag aatttcgat gattacgac 
      gacgaaacc ttcgacagt gacgaccct ttcgccgac 
      gaatggcaa aacgaagaa gccaccgag cccccagct 
      cctgtctct tctttcacc aaatacaag tgggacaat 
      tacggcact cgctcaagg gttgaagaa agcagacgg 
      cagcagctt catctcaag ccccataac gaaggaaga 
      gcaataaac gccgcattg gagcactac gcgaaggtc 
      cgccaagaa ggccaatgt cgcaaacca ttaccaaaa 
      gtgatctca gtccaaagc gaacatccc gatcctagc 
      aaaacctac actcctcat tgcaccatc ctgcatcgt 
      tgcgccgat gacacagga tgttgcgct aaccacacc 
      accagatgt gggcccaaa acccaagtc ctcgttcac 
      ctttacttt tacgcgaaa accttaggc gtgcccgga 
      accaaagta gaaaagttg actttttac aatcacacg 
      gaatgtgct tgtatcgaa aaatcgaaa gggccgaca 
      acactaccc gatgaaaat attaacttg actcacgga 
      ctatccctc aagcagggc tccgaaccg gaaaacatg 
      cgaaactgc aaatgcccg gaggagttc accccgaaa 
      ttccggtac gattccaga tgcagttgc gattgcgag 
      gagtataat caggactgc atcaggatg aagaagggg 
      aaggagtac ttcagtctc actgacaga aggtgcatc 
      atcgagaac caatgcgga attccggtt tgcgagtat 
      ggggtttac atgcgccat atgggtcgt tgccaacga 
      aaacaggag aaattcgat agttttagc aaaatgacc 
      gtgaaatga
    mRNA sequence
    • >TC008417 RA
      taatgataa taaaacgaa atgacctga ggtgcttat 
      tttacaaga agcgtattc caggaacag tgtccgccg 
      acttttagt gcaaattgg gtaaattgc aagcgcaca 
      taatcgtaa aatattgtc caaagtctc gataagagc 
      gacgtttaa taaattcgc gtatttaat ggccccggt 
      gcaaatccg tcccgattt tatgggacc ggataataa 
      caccgcaat gccgtggtg gtaaaaatc cttcgacaa 
      attacaatg cttccatac gtgtttatg cttcctcct 
      gaaatacga gatcagaca gttcgggag atgaatgga 
      ccacaatga atgcgcttt aacaaataa agagacacc 
      acaggctca gtcgatccg ttggcgcga cttcgaaag 
      cactacgcg tacgggaat ctcgcacaa acacgcgat 
      tatttttta aaatgcaga aaatgcaac ggttgtgtg 
      agtgtgtat ccggtctga ctcgaaatt aagcaattt 
      tcgccggaa aaatggcat gaatttgtc gtaaatcgt 
      cggcgcgtc ctttccaaa ctaacgata atgtgtaag 
      aaataaaca caaaatgtg gatcgccga tttgagaat 
      agtgctggg cttctgcat ctgtacggc tttggccta 
      ccaaggatc caagcagga ttacatcaa atggcggga 
      gcggttcct tccgaagag gccgacgac gacggagag 
      tgcagtgac tagtgcaaa cgatatcca ctacgaaaa 
      tctgaaatt gaggcacca ttcgcgctt cccgaattt 
      ggacagagt tctggtcaa gatagacaa tttgcagaa 
      gaagaacga cgtgcagcc ggttcataa taggattag 
      gcaccagaa cgtgtacaa gaccaccac cacgaccac 
      caccacgac tgacgaagg ccttttcgg cgacgatta 
      ttccgatcc ggagtacga agatgacaa caacgatga 
      gctcaatga ctacgacga gtgggacga cgagaattt 
      cgatgatta cgacgacga aaccttcga cagtgacga 
      ccctttcgc cgacgaatg gcaaaacga agaagccac 
      cgagccccc agctcctgt ctcttcttt caccaaata 
      caagtggga caattacgg cactcgctc aagggttga 
      agaaagcag acggcagca gcttcatct caagcccca 
      taacgaagg aagagcaat aaacgccgc attggagca 
      ctacgcgaa ggtccgcca agaaggcca atgtcgcaa 
      accattacc aaaagtgat ctcagtcca aagcgaaca 
      tcccgatcc tagcaaaac ctacactcc tcattgcac 
      catcctgca tcgttgcgc cgatgacac aggatgttg 
      cgctaacca caccaccag atgtgggcc caaaaccca 
      agtcctcgt tcaccttta cttttacgc gaaaacctt 
      aggcgtgcc cggaaccaa agtagaaaa gttgacttt 
      ttacaatca cacggaatg tgcttgtat cgaaaaatc 
      gaaagggcc gacaacact acccgatga aaatattaa 
      cttgactca cggactatc cctcaagca gggctccga 
      accggaaaa catgcgaaa ctgcaaatg cccggagga 
      gttcacccc gaaattccg gtacgattc cagatgcag 
      ttgcgattg cgaggagta taatcagga ctgcatcag 
      gatgaagaa ggggaagga gtacttcag tctcactga 
      cagaaggtg catcatcga gaaccaatg cggaattcc 
      ggtttgcga gtatggggt ttacatgcg ccatatggg 
      tcgttgcca acgaaaaca ggagaaatt cgatagttt 
      tagcaaaat gaccgtgaa atgaatcgt attattaaa 
      tttagtgta attaaagca attttgcca gtgatatat 
      tagtgaatt tatttgacg gtgacttta atttgtgtg 
      aaatgtggg tttccgcaa ttcggagcg cactgaatt 
      ggcggaaat ttacaagaa ttcgtgtac ggtgcatgt 
      gtttattgt ataagtgtc tttttttta ttgtacccg 
      aaacctagt ctttaggtc aaattaggt gctgtattt 
      aaaagcttc gatgattaa tataactgt ctcaaggaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY