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Gene information
General information
TC008414 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9964632..9969867
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008414
    iBeetle screen iB_04482

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: upended larvae show also missing abdominal and thoracic segments.  
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008414 RA
      MPPRWHRLS EIATCRRIL FAMLTSGFI VNTFVKAIF 
      NIAIVAMVA KDQPNTFDW TEGQKQEIL GIFFWGFAI 
      TKFPSGRLA EIIGSRKVT GYSMAVASV LTVLTPLIA 
      SFNYYALLV SRIVMGFVI GASWPALMP LVSNWIGPT 
      EHSMFMTAL ASTSIGFGL SSLVGGVLI ANYGWPSIF 
      YTAGSVALL WTILWFYFI YDSPRQHPR ISLEEREDI 
      ESKIKKDFG KIKLKFNDI PWRKIFVSG PVWGIAIAQ 
      MASFFTTMT ITTELPSYL DQVLHYDIS ENGVMASLP 
      TWGVYVTSL GSAYIADRL LQTNTLSTT TVRKTFGTT 
      AFLIPSIFM VILAFYGDV ATIVLVSFC ISLAINAFA 
      VAGHCANML DISPNFSGT ICGLVNTVA AITVYFSTR 
      IVGLLIRNG HTYENWRPL FWILAAVYG FGAVSYFVL 
      CSGKVQSWD LREPEREPL KKDNRGESP
    CDS sequence
    • >TC008414 RA
      atgcctcca cgatggcac cgactttca gagattgcc 
      acttgtcgc cgcatttta ttcgcaatg ctaaccagc 
      ggttttatt gtaaatacg ttcgttaaa gctattttt 
      aacatagct atagtcgca atggtcgcc aaagaccaa 
      cccaacact tttgactgg actgaaggc cagaagcag 
      gaaatactc gggattttc ttttggggt tttgctatc 
      acgaaattt ccgagcgga agattagcc gagataatc 
      ggttcgcgt aaagtaacc ggatattca atggctgtt 
      gcttcggtt ttaaccgtc ctgacacca cttatcgca 
      tctttcaac tactacgcc ttgttggtc tcccgaatt 
      gtgatgggg tttgtgatt ggggccagt tggcccgct 
      ttgatgcct ctggtctcg aactggatc ggaccaaca 
      gaacattca atgtttatg acggcgtta gcttcgact 
      tccattggg ttcggctta tcgtctctt gtaggtggt 
      gtcctcatt gcgaattac ggctggccc agtattttt 
      tacactgct ggaagtgtt gccctttta tggacgatt 
      ttgtggttt tatttcatc tatgattcg ccacgacaa 
      catccaaga atatcgctt gaagagagg gaagatatt 
      gaaagcaaa ataaaaaaa gattttggg aaaattaaa 
      ctaaagttc aatgatatc ccctggcgc aaaattttt 
      gtgtctggt ccagtgtgg ggcattgca attgcccaa 
      atggccagt tttttcacc acaatgacc ataacaacg 
      gaattacct tcttatctg gaccaagtt ttgcattac 
      gacatttca gaaaacggc gtgatggcg agtttgcca 
      acttggggg gtttatgtg acatcgtta ggaagtgcg 
      tacatcgct gaccgcctt ctgcaaacc aacactttg 
      tcgacaacc acggtgcga aaaactttc gggacgact 
      gccttcctc atccctagc atattcatg gtgattttg 
      gccttctac ggggatgta gccacgatt gttttagtt 
      tcgttctgt atcagtttg gccataaat gcgtttgct 
      gtggccggc cactgtgcg aatatgttg gacatttct 
      ccgaacttt tccggaact atttgcggt ctagtcaat 
      actgttgca gcaatcaca gtgtatttt tcaacaagg 
      attgttggg ttgttgata cgaaatggt cacacgtac 
      gaaaactgg cgaccactg ttttggatt ttggccgcg 
      gtttatggg ttcggagcc gtgagctat tttgtcctg 
      tgttcgggg aaagtgcaa agttgggac ttgagggag 
      cccgagagg gaacccctc aagaaggac aaccgcggg 
      gagagcccc tga
    mRNA sequence
    • >TC008414 RA
      gtaaacaaa attgacgaa actcgtcta atctgcatt 
      cgcggcttt gcctcttcc tctgcatta acaaccgca 
      accgcacgt atcattttc gataaatta ttccaatta 
      aaatcatgc ctccacgat ggcaccgac tttcagaga 
      ttgccactt gtcgccgca ttttattcg caatgctaa 
      ccagcggtt ttattgtaa atacgttcg ttaaagcta 
      tttttaaca tagctatag tcgcaatgg tcgccaaag 
      accaaccca acacttttg actggactg aaggccaga 
      agcaggaaa tactcggga ttttctttt ggggttttg 
      ctatcacga aatttccga gcggaagat tagccgaga 
      taatcggtt cgcgtaaag taaccggat attcaatgg 
      ctgttgctt cggttttaa ccgtcctga caccactta 
      tcgcatctt tcaactact acgccttgt tggtctccc 
      gaattgtga tggggtttg tgattgggg ccagttggc 
      ccgctttga tgcctctgg tctcgaact ggatcggac 
      caacagaac attcaatgt ttatgacgg cgttagctt 
      cgacttcca ttgggttcg gcttatcgt ctcttgtag 
      gtggtgtcc tcattgcga attacggct ggcccagta 
      ttttttaca ctgctggaa gtgttgccc ttttatgga 
      cgattttgt ggttttatt tcatctatg attcgccac 
      gacaacatc caagaatat cgcttgaag agagggaag 
      atattgaaa gcaaaataa aaaaagatt ttgggaaaa 
      ttaaactaa agttcaatg atatcccct ggcgcaaaa 
      tttttgtgt ctggtccag tgtggggca ttgcaattg 
      cccaaatgg ccagttttt tcaccacaa tgaccataa 
      caacggaat taccttctt atctggacc aagttttgc 
      attacgaca tttcagaaa acggcgtga tggcgagtt 
      tgccaactt ggggggttt atgtgacat cgttaggaa 
      gtgcgtaca tcgctgacc gccttctgc aaaccaaca 
      ctttgtcga caaccacgg tgcgaaaaa ctttcggga 
      cgactgcct tcctcatcc ctagcatat tcatggtga 
      ttttggcct tctacgggg atgtagcca cgattgttt 
      tagtttcgt tctgtatca gtttggcca taaatgcgt 
      ttgctgtgg ccggccact gtgcgaata tgttggaca 
      tttctccga acttttccg gaactattt gcggtctag 
      tcaatactg ttgcagcaa tcacagtgt atttttcaa 
      caaggattg ttgggttgt tgatacgaa atggtcaca 
      cgtacgaaa actggcgac cactgtttt ggattttgg 
      ccgcggttt atgggttcg gagccgtga gctattttg 
      tcctgtgtt cggggaaag tgcaaagtt gggacttga 
      gggagcccg agagggaac ccctcaaga aggacaacc 
      gcggggaga gcccctgaa agtattaac tgtgccgtt 
      ttgtaaata acactttat tttttaaaa atcattgat 
      ttttaatta caaaataca tacgtaatt agatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY