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Gene information
General information
TC008412 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9952472..9957968
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008412
    iBeetle screen iB_04481

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008412 RA
      MISSAFSIA IVAMFEEDG TSINLNVDE KHEVLGIYF 
      LGYMITKIP SGRLADTFG ARKVVGFST ILAALLTLA 
      TPFIVQWDY YALLGTRLM TGVLEGCIF PCLLPITAN 
      WYSTTPSSK FLSSMEASA FGIGFTFLI GGYLIDVCG 
      WASIFYFVG SVTLIWVGV WFYLVYDSP NEHPRITPD 
      ERIQIGREI ANFEIKKYS LFEIPWKGI LTSGPVWAI 
      VIAQVANFY LYAILTNEV PTYLSEVFQ YDIAEVGLF 
      ASLPPLGKY FVTILFGNL ADYSLSSGK LSVGATRKI 
      FEALGLLVP AGCLAIQAI FGHSLAVSL LTLMSAKLF 
      NGAIVGGHL PSIVEVAPN FSGFVSGWV FTFAAIAAY 
      SSAKITALL LQNGHTFDE WKYTFWITA GICLIGFVW 
      AIVIAQVAN FYLYAILTN EVPTYLSEV LQYDIAEVG 
      LFASLPPLG KYFVTILFG NLADYSLSS GKLSVGATR 
      KIFEALGLL VPAGCLAIQ AIFGHSLAV SLLTLMSAK 
      LFNGAIVGG HLPSIVEVA PNFSGFVSG WVFTFAAIA 
      AYSSAKITA LLLQNGHTF DEWKYTFWI TAGICLIGF 
      VWAIVIAQV ANFYLYAIL TNEVPTYLS EVLQYDIAE 
      VGLFASLPP LGKYFVTIL FGNLADYSL NSGKLLVGT 
      TRKIFEALG LLVPAGCLA IQAIFGHSL AVSLLTLMS 
      AKLFNGAIV GGHLPSIVE VAPNFSGFV SGNAMPPIH 
      EMLTCRRIL TIMSVSGFI VNAVLKTVF NIAVVAMTK 
      KTNQTAEIF AWDENQRQE LIGIFFWGF ALTKIPSGR 
      LAEVIGSRK VTGYSMLLA SLLTILTPW ISYSNYYIL 
      LSSRVLMGF LVGASWPAV MPLAARWVP PHEQTIFIS 
      CIASTAVGA GIAFQVSGF LISALGWKS VFYVFGGVS 
      TLWSLLWFG LIYDSPQQH PRISQEEKD LIESQIRDV 
      KIRNVRLSE IPWISILTS GPVWAIAAG QIAVFFGYM 
      TLSNEIPSY MDQVLHLEI KQIGIFAGF PYFGAYFLS 
      LASSHFADY LRKSGKLST TAVRKIFEA VGLLVPAFS 
      MLLLVFWGY LTPVAVTLF TISITTCAI SSAGHCTNM 
      LDVSPNFAG TICGLVNTF SSFTAYIST EMVTALLRK 
      DNTFKEWRF VFAIVFGVE VIATGVYLI FCSGEKQSW 
      DSVKTNEGR EEIRPLKRK NLNQNLE
    CDS sequence
    • >TC008412 RA
      atgattagt tcggctttc agcattgcc atagttgcg 
      atgtttgaa gaggatggt acttcgatt aatttgaat 
      gtggatgag aagcatgag gttttgggt atttatttc 
      ctaggatat atgattacg aaaatacca agtggaaga 
      ttggctgat acatttggg gcaagaaaa gtagtaggg 
      tttagtacc atcctggct gctctttta acccttgca 
      actcctttt attgtgcaa tgggattat tacgcactt 
      ttgggcaca agactgatg actggggtg ttagaaggg 
      tgcatattt ccatgttta ctacccatc acagccaat 
      tggtactcc actacaccg tcttcgaaa tttttgtca 
      agtatggaa gcctctgcc tttggtata ggtttcact 
      tttctaatt ggtggatac cttattgat gtgtgtggc 
      tgggctagt attttttac tttgtcgga agtgtaaca 
      ctgatatgg gtcggggtt tggttctac ttggtgtac 
      gacagtccg aatgaacat cccagaata actcccgac 
      gaaagaatt caaattggg cgagagatt gcaaatttt 
      gaaataaaa aagtatagt ttatttgaa attccatgg 
      aaaggcatt cttacttct ggcccagtc tgggccata 
      gtcatagct caagtggca aatttttac ctttatgcc 
      attttaacc aatgaggta ccaacttat ctaagtgaa 
      gtcttccaa tacgacatt gccgaagtc gggttattt 
      gcaagttta cccccttta ggcaaatat ttcgtaacg 
      atacttttt ggaaactta gccgattat tcgctaagc 
      tctgggaaa ttgtcagtt ggtgcaacg agaaaaatc 
      tttgaagct ttaggattg ctggttccc gccggttgt 
      ctggcaatt caagcaatc tttggacac agtttagct 
      gtatcttta ttgacttta atgagtgca aaattgttt 
      aatggagct attgttggt ggtcacctt ccaagcata 
      gtagaagtt gctccaaac ttttcagga tttgtgtca 
      gggtgggtt tttactttt gccgcaatc gcagcgtat 
      tcatcggcg aaaattacc gccctttta cttcaaaat 
      ggacacact ttcgacgaa tggaagtac actttttgg 
      atcacggcc ggaatttgc ctcatcgga ttcgtctgg 
      gcaatagtc atagctcaa gtggcaaat ttttacctt 
      tatgccatt ttaaccaat gaggtacca acttatcta 
      agtgaagtc ttgcaatac gacattgcc gaagtcggg 
      ttatttgca agtttaccg cctttgggc aaatatttc 
      gtaacgata ctttttgga aacttagcc gattattcg 
      ctaagctct gggaaattg tcagttggt gcaacgaga 
      aaaatcttc gaagcttta ggattgctg gttcccgcc 
      ggttgtctg gcaattcaa gcaatcttt ggacacagt 
      ttagctgtg tctttattg actttaatg agtgcaaaa 
      ttgtttaat ggagctatt gttggtggt caccttcca 
      agcatagta gaagttgct ccaaacttt tcaggattt 
      gtgtcaggg tgggttttt acttttgcc gcaatcgca 
      gcgtattca tcggcgaaa attaccgcc cttctgctt 
      caaaatgga cacactttc gacgaatgg aagtacact 
      ttctggatc acggccgga atttgcctc atcggattc 
      gtctgggca atagtcata gctcaagtg gcaaatttt 
      tacctttat gccatttta accaatgag gtaccaact 
      tatctaagt gaagtcttg caatacgac attgccgaa 
      gtcgggtta tttgcaagt ttaccgcct ttgggcaaa 
      tatttcgta acgatactt tttggaaac ttagccgat 
      tattcgcta aactctggg aaattgtta gttggtaca 
      actagaaaa atctttgaa gctttagga ttgctggtt 
      cctgccggt tgtctggca attcaagca atctttgga 
      cacagttta gctgtgtct ttattgact ttaatgagt 
      gcaaaattg tttaatgga gctattgtt ggtggtcac 
      cttccaagc atagtagaa gttgctcca aacttttca 
      ggatttgta tcaggaaac gcaatgcct cccatccat 
      gaaatgctg acctgccgc cgaatctta accataatg 
      tctgtaagt ggttttatc gtaaacgcg gtcctgaaa 
      accgttttc aacattgca gtggtcgca atgaccaag 
      aagaccaat caaacggcg gaaattttt gcctgggac 
      gaaaaccag cgccaggaa ctcatcggg atttttttc 
      tggggtttt gccctaact aaaatcccc agtggacgt 
      ctagctgaa gtaatcggc tcgcgaaaa gttacgggt 
      tactccatg ttgctagcc tcactcctc acaattctc 
      actccttgg atttcttac tcaaattat tacatactt 
      ttatcttct cgcgttttg atgggtttt cttgtgggg 
      gctagttgg ccggctgtt atgcccttg gcggcaagg 
      tgggtacca ccccatgag caaacaatt ttcatttct 
      tgcatcgcc tcaacagct gtgggagcg ggaattgcg 
      ttccaagtt tccgggttc ctcatttct gcactaggt 
      tggaaaagt gttttttat gtttttggg ggcgtatca 
      acgttgtgg tctctactt tggtttggt ttaatttac 
      gactcccca caacaacat ccccgaatt tcgcaagaa 
      gagaaagat ttgatcgag agccagatt agagacgta 
      aaaatccga aacgtgaga ttgagtgaa attccctgg 
      atcagtatt ttgacgtct ggtcccgtg tgggccatt 
      gccgcaggc caaattgca gttttcttc ggttatatg 
      actctgagt aacgaaata ccctcctac atggaccaa 
      gtcctccac ttagagatc aaacaaata gggattttt 
      gccggtttt ccctacttc ggggcttac tttttgtcc 
      ctagcttca agccacttt gccgattat ttgcgcaaa 
      agtgggaaa ttgtccaca acagctgtt cgaaaaatc 
      ttcgaggca gttggatta ctagttccg gccttttcg 
      atgctactt ttagtgttt tgggggtat ttgacccca 
      gttgctgtc actcttttt acaatttcg attacaaca 
      tgtgccatt tcaagcgct ggtcattgt acaaacatg 
      ttggacgtt tcaccgaac ttcgcaggg accatttgt 
      ggtctggtt aacactttt tcgtcattt acggcctac 
      atttcgacg gaaatggtc acagctttg ctcagaaaa 
      gacaacact tttaaagaa tggcgcttc gtttttgct 
      attgttttc ggggttgag gtgattgcg actggggtt 
      tatttgatt ttttgctcg ggggaaaaa caaagttgg 
      gattcggtc aagacaaat gaaggaaga gaagaaata 
      aggccacta aagaggaaa aatttgaac caaaatctt 
      gaataa
    mRNA sequence
    • >TC008412 RA
      tctgtgtgg gatagcacc cattgtgag cacctctcg 
      tctagacaa tcgaaagac attaattaa catgccgga 
      aaattttat acgttgttt tggctttcg gatttatga 
      cacattcaa tgattagtt cggctttca gcattgcca 
      tagttgcga tgtttgaag aggatggta cttcgatta 
      atttgaatg tggatgaga agcatgagg ttttgggta 
      tttatttcc taggatata tgattacga aaataccaa 
      gtggaagat tggctgata catttgggg caagaaaag 
      tagtagggt ttagtacca tcctggctg ctcttttaa 
      cccttgcaa ctcctttta ttgtgcaat gggattatt 
      acgcacttt tgggcacaa gactgatga ctggggtgt 
      tagaagggt gcatatttc catgtttac tacccatca 
      cagccaatt ggtactcca ctacaccgt cttcgaaat 
      ttttgtcaa gtatggaag cctctgcct ttggtatag 
      gtttcactt ttctaattg gtggatacc ttattgatg 
      tgtgtggct gggctagta ttttttact ttgtcggaa 
      gtgtaacac tgatatggg tcggggttt ggttctact 
      tggtgtacg acagtccga atgaacatc ccagaataa 
      ctcccgacg aaagaattc aaattgggc gagagattg 
      caaattttg aaataaaaa agtatagtt tatttgaaa 
      ttccatgga aaggcattc ttacttctg gcccagtct 
      gggccatag tcatagctc aagtggcaa atttttacc 
      tttatgcca ttttaacca atgaggtac caacttatc 
      taagtgaag tcttccaat acgacattg ccgaagtcg 
      ggttatttg caagtttac cccctttag gcaaatatt 
      tcgtaacga tactttttg gaaacttag ccgattatt 
      cgctaagct ctgggaaat tgtcagttg gtgcaacga 
      gaaaaatct ttgaagctt taggattgc tggttcccg 
      ccggttgtc tggcaattc aagcaatct ttggacaca 
      gtttagctg tatctttat tgactttaa tgagtgcaa 
      aattgttta atggagcta ttgttggtg gtcaccttc 
      caagcatag tagaagttg ctccaaact tttcaggat 
      ttgtgtcag ggtgggttt ttacttttg ccgcaatcg 
      cagcgtatt catcggcga aaattaccg cccttttac 
      ttcaaaatg gacacactt tcgacgaat ggaagtaca 
      ctttttgga tcacggccg gaatttgcc tcatcggat 
      tcgtctggg caatagtca tagctcaag tggcaaatt 
      tttaccttt atgccattt taaccaatg aggtaccaa 
      cttatctaa gtgaagtct tgcaatacg acattgccg 
      aagtcgggt tatttgcaa gtttaccgc ctttgggca 
      aatatttcg taacgatac tttttggaa acttagccg 
      attattcgc taagctctg ggaaattgt cagttggtg 
      caacgagaa aaatcttcg aagctttag gattgctgg 
      ttcccgccg gttgtctgg caattcaag caatctttg 
      gacacagtt tagctgtgt ctttattga ctttaatga 
      gtgcaaaat tgtttaatg gagctattg ttggtggtc 
      accttccaa gcatagtag aagttgctc caaactttt 
      caggatttg tgtcagggt gggttttta cttttgccg 
      caatcgcag cgtattcat cggcgaaaa ttaccgccc 
      ttctgcttc aaaatggac acactttcg acgaatgga 
      agtacactt tctggatca cggccggaa tttgcctca 
      tcggattcg tctgggcaa tagtcatag ctcaagtgg 
      caaattttt acctttatg ccattttaa ccaatgagg 
      taccaactt atctaagtg aagtcttgc aatacgaca 
      ttgccgaag tcgggttat ttgcaagtt taccgcctt 
      tgggcaaat atttcgtaa cgatacttt ttggaaact 
      tagccgatt attcgctaa actctggga aattgttag 
      ttggtacaa ctagaaaaa tctttgaag ctttaggat 
      tgctggttc ctgccggtt gtctggcaa ttcaagcaa 
      tctttggac acagtttag ctgtgtctt tattgactt 
      taatgagtg caaaattgt ttaatggag ctattgttg 
      gtggtcacc ttccaagca tagtagaag ttgctccaa 
      acttttcag gatttgtat caggaaacg caatgcctc 
      ccatccatg aaatgctga cctgccgcc gaatcttaa 
      ccataatgt ctgtaagtg gttttatcg taaacgcgg 
      tcctgaaaa ccgttttca acattgcag tggtcgcaa 
      tgaccaaga agaccaatc aaacggcgg aaatttttg 
      cctgggacg aaaaccagc gccaggaac tcatcggga 
      tttttttct ggggttttg ccctaacta aaatcccca 
      gtggacgtc tagctgaag taatcggct cgcgaaaag 
      ttacgggtt actccatgt tgctagcct cactcctca 
      caattctca ctccttgga tttcttact caaattatt 
      acatacttt tatcttctc gcgttttga tgggttttc 
      ttgtggggg ctagttggc cggctgtta tgcccttgg 
      cggcaaggt gggtaccac cccatgagc aaacaattt 
      tcatttctt gcatcgcct caacagctg tgggagcgg 
      gaattgcgt tccaagttt ccgggttcc tcatttctg 
      cactaggtt ggaaaagtg ttttttatg tttttgggg 
      gcgtatcaa cgttgtggt ctctacttt ggtttggtt 
      taatttacg actccccac aacaacatc cccgaattt 
      cgcaagaag agaaagatt tgatcgaga gccagatta 
      gagacgtaa aaatccgaa acgtgagat tgagtgaaa 
      ttccctgga tcagtattt tgacgtctg gtcccgtgt 
      gggccattg ccgcaggcc aaattgcag ttttcttcg 
      gttatatga ctctgagta acgaaatac cctcctaca 
      tggaccaag tcctccact tagagatca aacaaatag 
      ggatttttg ccggttttc cctacttcg gggcttact 
      ttttgtccc tagcttcaa gccactttg ccgattatt 
      tgcgcaaaa gtgggaaat tgtccacaa cagctgttc 
      gaaaaatct tcgaggcag ttggattac tagttccgg 
      ccttttcga tgctacttt tagtgtttt gggggtatt 
      tgaccccag ttgctgtca ctcttttta caatttcga 
      ttacaacat gtgccattt caagcgctg gtcattgta 
      caaacatgt tggacgttt caccgaact tcgcaggga 
      ccatttgtg gtctggtta acacttttt cgtcattta 
      cggcctaca tttcgacgg aaatggtca cagctttgc 
      tcagaaaag acaacactt ttaaagaat ggcgcttcg 
      tttttgcta ttgttttcg gggttgagg tgattgcga 
      ctggggttt atttgattt tttgctcgg gggaaaaac 
      aaagttggg attcggtca agacaaatg aaggaagag 
      aagaaataa ggccactaa agaggaaaa atttgaacc 
      aaaatcttg aataaattt tgttattaa taccgtttt 
      gcttttatt acgttggaa agaaagctt tgccccttt 
      t
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