| General information | |||
| TC008412 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9952472..9957968 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008412 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04481 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC008412 RA
MISSAFSIA IVAMFEEDG TSINLNVDE KHEVLGIYF LGYMITKIP SGRLADTFG ARKVVGFST ILAALLTLA TPFIVQWDY YALLGTRLM TGVLEGCIF PCLLPITAN WYSTTPSSK FLSSMEASA FGIGFTFLI GGYLIDVCG WASIFYFVG SVTLIWVGV WFYLVYDSP NEHPRITPD ERIQIGREI ANFEIKKYS LFEIPWKGI LTSGPVWAI VIAQVANFY LYAILTNEV PTYLSEVFQ YDIAEVGLF ASLPPLGKY FVTILFGNL ADYSLSSGK LSVGATRKI FEALGLLVP AGCLAIQAI FGHSLAVSL LTLMSAKLF NGAIVGGHL PSIVEVAPN FSGFVSGWV FTFAAIAAY SSAKITALL LQNGHTFDE WKYTFWITA GICLIGFVW AIVIAQVAN FYLYAILTN EVPTYLSEV LQYDIAEVG LFASLPPLG KYFVTILFG NLADYSLSS GKLSVGATR KIFEALGLL VPAGCLAIQ AIFGHSLAV SLLTLMSAK LFNGAIVGG HLPSIVEVA PNFSGFVSG WVFTFAAIA AYSSAKITA LLLQNGHTF DEWKYTFWI TAGICLIGF VWAIVIAQV ANFYLYAIL TNEVPTYLS EVLQYDIAE VGLFASLPP LGKYFVTIL FGNLADYSL NSGKLLVGT TRKIFEALG LLVPAGCLA IQAIFGHSL AVSLLTLMS AKLFNGAIV GGHLPSIVE VAPNFSGFV SGNAMPPIH EMLTCRRIL TIMSVSGFI VNAVLKTVF NIAVVAMTK KTNQTAEIF AWDENQRQE LIGIFFWGF ALTKIPSGR LAEVIGSRK VTGYSMLLA SLLTILTPW ISYSNYYIL LSSRVLMGF LVGASWPAV MPLAARWVP PHEQTIFIS CIASTAVGA GIAFQVSGF LISALGWKS VFYVFGGVS TLWSLLWFG LIYDSPQQH PRISQEEKD LIESQIRDV KIRNVRLSE IPWISILTS GPVWAIAAG QIAVFFGYM TLSNEIPSY MDQVLHLEI KQIGIFAGF PYFGAYFLS LASSHFADY LRKSGKLST TAVRKIFEA VGLLVPAFS MLLLVFWGY LTPVAVTLF TISITTCAI SSAGHCTNM LDVSPNFAG TICGLVNTF SSFTAYIST EMVTALLRK DNTFKEWRF VFAIVFGVE VIATGVYLI FCSGEKQSW DSVKTNEGR EEIRPLKRK NLNQNLE
- >TC008412 RA
atgattagt tcggctttc agcattgcc atagttgcg atgtttgaa gaggatggt acttcgatt aatttgaat gtggatgag aagcatgag gttttgggt atttatttc ctaggatat atgattacg aaaatacca agtggaaga ttggctgat acatttggg gcaagaaaa gtagtaggg tttagtacc atcctggct gctctttta acccttgca actcctttt attgtgcaa tgggattat tacgcactt ttgggcaca agactgatg actggggtg ttagaaggg tgcatattt ccatgttta ctacccatc acagccaat tggtactcc actacaccg tcttcgaaa tttttgtca agtatggaa gcctctgcc tttggtata ggtttcact tttctaatt ggtggatac cttattgat gtgtgtggc tgggctagt attttttac tttgtcgga agtgtaaca ctgatatgg gtcggggtt tggttctac ttggtgtac gacagtccg aatgaacat cccagaata actcccgac gaaagaatt caaattggg cgagagatt gcaaatttt gaaataaaa aagtatagt ttatttgaa attccatgg aaaggcatt cttacttct ggcccagtc tgggccata gtcatagct caagtggca aatttttac ctttatgcc attttaacc aatgaggta ccaacttat ctaagtgaa gtcttccaa tacgacatt gccgaagtc gggttattt gcaagttta cccccttta ggcaaatat ttcgtaacg atacttttt ggaaactta gccgattat tcgctaagc tctgggaaa ttgtcagtt ggtgcaacg agaaaaatc tttgaagct ttaggattg ctggttccc gccggttgt ctggcaatt caagcaatc tttggacac agtttagct gtatcttta ttgacttta atgagtgca aaattgttt aatggagct attgttggt ggtcacctt ccaagcata gtagaagtt gctccaaac ttttcagga tttgtgtca gggtgggtt tttactttt gccgcaatc gcagcgtat tcatcggcg aaaattacc gccctttta cttcaaaat ggacacact ttcgacgaa tggaagtac actttttgg atcacggcc ggaatttgc ctcatcgga ttcgtctgg gcaatagtc atagctcaa gtggcaaat ttttacctt tatgccatt ttaaccaat gaggtacca acttatcta agtgaagtc ttgcaatac gacattgcc gaagtcggg ttatttgca agtttaccg cctttgggc aaatatttc gtaacgata ctttttgga aacttagcc gattattcg ctaagctct gggaaattg tcagttggt gcaacgaga aaaatcttc gaagcttta ggattgctg gttcccgcc ggttgtctg gcaattcaa gcaatcttt ggacacagt ttagctgtg tctttattg actttaatg agtgcaaaa ttgtttaat ggagctatt gttggtggt caccttcca agcatagta gaagttgct ccaaacttt tcaggattt gtgtcaggg tgggttttt acttttgcc gcaatcgca gcgtattca tcggcgaaa attaccgcc cttctgctt caaaatgga cacactttc gacgaatgg aagtacact ttctggatc acggccgga atttgcctc atcggattc gtctgggca atagtcata gctcaagtg gcaaatttt tacctttat gccatttta accaatgag gtaccaact tatctaagt gaagtcttg caatacgac attgccgaa gtcgggtta tttgcaagt ttaccgcct ttgggcaaa tatttcgta acgatactt tttggaaac ttagccgat tattcgcta aactctggg aaattgtta gttggtaca actagaaaa atctttgaa gctttagga ttgctggtt cctgccggt tgtctggca attcaagca atctttgga cacagttta gctgtgtct ttattgact ttaatgagt gcaaaattg tttaatgga gctattgtt ggtggtcac cttccaagc atagtagaa gttgctcca aacttttca ggatttgta tcaggaaac gcaatgcct cccatccat gaaatgctg acctgccgc cgaatctta accataatg tctgtaagt ggttttatc gtaaacgcg gtcctgaaa accgttttc aacattgca gtggtcgca atgaccaag aagaccaat caaacggcg gaaattttt gcctgggac gaaaaccag cgccaggaa ctcatcggg atttttttc tggggtttt gccctaact aaaatcccc agtggacgt ctagctgaa gtaatcggc tcgcgaaaa gttacgggt tactccatg ttgctagcc tcactcctc acaattctc actccttgg atttcttac tcaaattat tacatactt ttatcttct cgcgttttg atgggtttt cttgtgggg gctagttgg ccggctgtt atgcccttg gcggcaagg tgggtacca ccccatgag caaacaatt ttcatttct tgcatcgcc tcaacagct gtgggagcg ggaattgcg ttccaagtt tccgggttc ctcatttct gcactaggt tggaaaagt gttttttat gtttttggg ggcgtatca acgttgtgg tctctactt tggtttggt ttaatttac gactcccca caacaacat ccccgaatt tcgcaagaa gagaaagat ttgatcgag agccagatt agagacgta aaaatccga aacgtgaga ttgagtgaa attccctgg atcagtatt ttgacgtct ggtcccgtg tgggccatt gccgcaggc caaattgca gttttcttc ggttatatg actctgagt aacgaaata ccctcctac atggaccaa gtcctccac ttagagatc aaacaaata gggattttt gccggtttt ccctacttc ggggcttac tttttgtcc ctagcttca agccacttt gccgattat ttgcgcaaa agtgggaaa ttgtccaca acagctgtt cgaaaaatc ttcgaggca gttggatta ctagttccg gccttttcg atgctactt ttagtgttt tgggggtat ttgacccca gttgctgtc actcttttt acaatttcg attacaaca tgtgccatt tcaagcgct ggtcattgt acaaacatg ttggacgtt tcaccgaac ttcgcaggg accatttgt ggtctggtt aacactttt tcgtcattt acggcctac atttcgacg gaaatggtc acagctttg ctcagaaaa gacaacact tttaaagaa tggcgcttc gtttttgct attgttttc ggggttgag gtgattgcg actggggtt tatttgatt ttttgctcg ggggaaaaa caaagttgg gattcggtc aagacaaat gaaggaaga gaagaaata aggccacta aagaggaaa aatttgaac caaaatctt gaataa
- >TC008412 RA
tctgtgtgg gatagcacc cattgtgag cacctctcg tctagacaa tcgaaagac attaattaa catgccgga aaattttat acgttgttt tggctttcg gatttatga cacattcaa tgattagtt cggctttca gcattgcca tagttgcga tgtttgaag aggatggta cttcgatta atttgaatg tggatgaga agcatgagg ttttgggta tttatttcc taggatata tgattacga aaataccaa gtggaagat tggctgata catttgggg caagaaaag tagtagggt ttagtacca tcctggctg ctcttttaa cccttgcaa ctcctttta ttgtgcaat gggattatt acgcacttt tgggcacaa gactgatga ctggggtgt tagaagggt gcatatttc catgtttac tacccatca cagccaatt ggtactcca ctacaccgt cttcgaaat ttttgtcaa gtatggaag cctctgcct ttggtatag gtttcactt ttctaattg gtggatacc ttattgatg tgtgtggct gggctagta ttttttact ttgtcggaa gtgtaacac tgatatggg tcggggttt ggttctact tggtgtacg acagtccga atgaacatc ccagaataa ctcccgacg aaagaattc aaattgggc gagagattg caaattttg aaataaaaa agtatagtt tatttgaaa ttccatgga aaggcattc ttacttctg gcccagtct gggccatag tcatagctc aagtggcaa atttttacc tttatgcca ttttaacca atgaggtac caacttatc taagtgaag tcttccaat acgacattg ccgaagtcg ggttatttg caagtttac cccctttag gcaaatatt tcgtaacga tactttttg gaaacttag ccgattatt cgctaagct ctgggaaat tgtcagttg gtgcaacga gaaaaatct ttgaagctt taggattgc tggttcccg ccggttgtc tggcaattc aagcaatct ttggacaca gtttagctg tatctttat tgactttaa tgagtgcaa aattgttta atggagcta ttgttggtg gtcaccttc caagcatag tagaagttg ctccaaact tttcaggat ttgtgtcag ggtgggttt ttacttttg ccgcaatcg cagcgtatt catcggcga aaattaccg cccttttac ttcaaaatg gacacactt tcgacgaat ggaagtaca ctttttgga tcacggccg gaatttgcc tcatcggat tcgtctggg caatagtca tagctcaag tggcaaatt tttaccttt atgccattt taaccaatg aggtaccaa cttatctaa gtgaagtct tgcaatacg acattgccg aagtcgggt tatttgcaa gtttaccgc ctttgggca aatatttcg taacgatac tttttggaa acttagccg attattcgc taagctctg ggaaattgt cagttggtg caacgagaa aaatcttcg aagctttag gattgctgg ttcccgccg gttgtctgg caattcaag caatctttg gacacagtt tagctgtgt ctttattga ctttaatga gtgcaaaat tgtttaatg gagctattg ttggtggtc accttccaa gcatagtag aagttgctc caaactttt caggatttg tgtcagggt gggttttta cttttgccg caatcgcag cgtattcat cggcgaaaa ttaccgccc ttctgcttc aaaatggac acactttcg acgaatgga agtacactt tctggatca cggccggaa tttgcctca tcggattcg tctgggcaa tagtcatag ctcaagtgg caaattttt acctttatg ccattttaa ccaatgagg taccaactt atctaagtg aagtcttgc aatacgaca ttgccgaag tcgggttat ttgcaagtt taccgcctt tgggcaaat atttcgtaa cgatacttt ttggaaact tagccgatt attcgctaa actctggga aattgttag ttggtacaa ctagaaaaa tctttgaag ctttaggat tgctggttc ctgccggtt gtctggcaa ttcaagcaa tctttggac acagtttag ctgtgtctt tattgactt taatgagtg caaaattgt ttaatggag ctattgttg gtggtcacc ttccaagca tagtagaag ttgctccaa acttttcag gatttgtat caggaaacg caatgcctc ccatccatg aaatgctga cctgccgcc gaatcttaa ccataatgt ctgtaagtg gttttatcg taaacgcgg tcctgaaaa ccgttttca acattgcag tggtcgcaa tgaccaaga agaccaatc aaacggcgg aaatttttg cctgggacg aaaaccagc gccaggaac tcatcggga tttttttct ggggttttg ccctaacta aaatcccca gtggacgtc tagctgaag taatcggct cgcgaaaag ttacgggtt actccatgt tgctagcct cactcctca caattctca ctccttgga tttcttact caaattatt acatacttt tatcttctc gcgttttga tgggttttc ttgtggggg ctagttggc cggctgtta tgcccttgg cggcaaggt gggtaccac cccatgagc aaacaattt tcatttctt gcatcgcct caacagctg tgggagcgg gaattgcgt tccaagttt ccgggttcc tcatttctg cactaggtt ggaaaagtg ttttttatg tttttgggg gcgtatcaa cgttgtggt ctctacttt ggtttggtt taatttacg actccccac aacaacatc cccgaattt cgcaagaag agaaagatt tgatcgaga gccagatta gagacgtaa aaatccgaa acgtgagat tgagtgaaa ttccctgga tcagtattt tgacgtctg gtcccgtgt gggccattg ccgcaggcc aaattgcag ttttcttcg gttatatga ctctgagta acgaaatac cctcctaca tggaccaag tcctccact tagagatca aacaaatag ggatttttg ccggttttc cctacttcg gggcttact ttttgtccc tagcttcaa gccactttg ccgattatt tgcgcaaaa gtgggaaat tgtccacaa cagctgttc gaaaaatct tcgaggcag ttggattac tagttccgg ccttttcga tgctacttt tagtgtttt gggggtatt tgaccccag ttgctgtca ctcttttta caatttcga ttacaacat gtgccattt caagcgctg gtcattgta caaacatgt tggacgttt caccgaact tcgcaggga ccatttgtg gtctggtta acacttttt cgtcattta cggcctaca tttcgacgg aaatggtca cagctttgc tcagaaaag acaacactt ttaaagaat ggcgcttcg tttttgcta ttgttttcg gggttgagg tgattgcga ctggggttt atttgattt tttgctcgg gggaaaaac aaagttggg attcggtca agacaaatg aaggaagag aagaaataa ggccactaa agaggaaaa atttgaacc aaaatcttg aataaattt tgttattaa taccgtttt gcttttatt acgttggaa agaaagctt tgccccttt t
