| General information | |||
| TC032946
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 9664163..9679180 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032946 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04479 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032946 RA
MAERSRKTL GRSASTEST EYSLTMGRS VRDKKKKPK ERWLLTRKT WRYMADAGR KLIPDGAQN RPEDIPKIE AYFQEVCKR EPKFLLWRK NSYPGALAF RKKVRKERR KGGSCRKAS SADSVDLIK LERPTDLAL PSTSGARYD IQKMKYDFL NKPSPTSLN TQEDLEEKQ LLSMLEQYL TISEQTAST SKYNPPDFN YQELIDKLQ RHLTLATRS FVNVPTYQS QSQVHFNGD HVQKSLSET LSRYFGQHT NRDKVISDL LTDRKALEK LYFDLRKAR GFRRNVSGS DYSSGISFK PNLSKYPIQ SSNYEKLKE RMYESHPPP LIEIENESA SVSYRSWGI QTLPVPESV LLEVEEKYK KAQAEKEEE ERRDLRPAG RRRSSVDND DISQSVSDT IKRYLRMAR KKSVDTDKV DRFKRVNYD RNLRNIKAK GEITKPGDD DGLNKGCQT NDDWILKYR DLKFDDFSD IESRLSSSR SSVDENNKS SPSSPPPTK SHSFLSHLL HKHDKHDKG NSPAVAAGP AMQKSKSSS SVMHHGSRL MAKKIFRSR SKSQTRPSQ ATCSWTPQG GCVWSNLTG RQVVLGTTS LLHLTDIER KVLQKVALA KLQALNLGV AIKVPTENV GAVVSKPKR RPYLLKRKA LTTSIFDTN RKEGDKEGG SAGGLVFGI PLTQCVEND RLTRAAART SPFRSGAEL SGSTEEPAT MGRHGSRSS FSSLIEAPQ GDESGSCES LMLRERLVG SVPGLLDTL SCGSTSDLP HEEEASVPN ILSECFRHL EQHGLHTLG IFRVSTSKK RIRQLREDF DCGKETSLD DDQCPHDVA TLLKEYLRD LPDPLLCRD LYQAFVQTQ RIRNRRLQF EALQHLIQL LPTANRDTL YALLGFLAT VAQNAADTK DESGECVSG NKMDSNNLA TVFAPNILH CIKPGSSKE IASDRPEDR IDIINVVRT LIDHYKQLY TISAELLDE IYVHMMDSH PEALDQLLN KRDALSGAD ESVEEVDSE SYSAPWSPT GPTPELPLE SEETKKMYS REEFLHERA AMGGPNVGM RIRHKDRIR ERSSKKRRD ESTSRKKKE DEGSIGSGI SSAMHIISR LRGQKDDDY QYSKNRSSS LESNSSAHT DSDINRVLR LEDDRRKSS PYILDNSGV ITASLTIPV QTATQPFPL NLDDDIPYI EDAENSRQQ ITIGLVRTP TAPPRRRQR SISGSDSSV ASNVQPITG SISLIQGYD SAVGSSATY SSPVQNTSS STAGDAAVQ TVNYMPEEL QRSAKKTPS AATLQKVSF SSTQEVQQI KPKSSKEQD LQKSFSVST FEQIKTEPK HTPSISSIG NAVLRSRTA DFERITKVE VQKPKASSE KKKYTKRRY TDSRHQTRH IPDSESLES ANAQSKKED TTIVAQAPV YKRRELISS VPSK
- >TC032946 RA
atggcggag cgttctcgg aaaacttta ggcaggagt gcctcaacc gaaagcacc gaatattcg ctaacaatg ggtcgctcg gtccgcgac aagaaaaag aaaccaaag gagcgctgg cttctaaca cgcaaaacc tggaggtac atggcggac gccggccgg aaactcatc ccagatggg gcccaaaac cggcccgaa gacatcccc aaaatcgag gcctacttc caggaggtg tgcaaacgg gagcccaag ttcctcctt tggcgcaaa aactcctat cctggagcc ttggccttc cggaagaaa gtccggaag gagcggcgc aaagggggc agttgtcgg aaagcctcc tcagctgac agtgtcgat ctgatcaag ttggaacga ccaacagac ttggcctta ccaagcaca agcggcgct cgatacgac atccagaaa atgaaatat gatttttta aacaaaccg tctccgacc agcttaaac acacaggag gatttggag gaaaaacag ctgttgagt atgctggaa caatacttg acgatttcg gaacaaacc gcctcgacc tcgaaatac aatcctccc gattttaat taccaggaa ttgatcgat aagttgcaa agacacttg actcttgca actaggagt tttgtcaat gttccgact tatcagagc caaagtcag gtgcatttt aacggcgat catgtccaa aaatcactc tcggagact ttgagtcgt tattttggc caacacacg aatcgggac aaagtcatt tctgattta ctcactgat cggaaagct ttggaaaag ctttatttt gatttgagg aaagctcgc ggttttagg aggaatgtg agtggaagt gattattca tcagggatt agttttaaa ccgaatttg tccaaatat ccgatccaa agttcgaat tatgagaaa ctgaaggaa cggatgtat gagtctcac ccacctccc ttaatcgaa attgaaaat gagtctgcg agtgttagt tatcgcagt tgggggatt cagacttta ccagtcccc gagtcagtt ctcctggag gttgaggag aagtacaag aaggctcag gccgagaag gaggaggag gagaggagg gatttgaga cctgcaggt cgtagacga agcagtgtc gataacgac gacatctcc caatccgtc agcgacaca attaagcgg tatttacgg atggcgcga aaaaagagc gtagacacg gacaaagtg gaccgcttc aaacgagtg aattacgac agaaacttg cggaacatc aaagcaaaa ggagaaata acaaaacca ggagacgac gacggtcta aataaaggt tgtcaaacc aatgacgat tggattttg aaataccgc gatttgaaa ttcgatgat ttttccgat attgaaagc cggctttct agttcgcga agtagtgtg gatgagaac aataaatcc tcccctagc tcaccacct ccgaccaaa tcccattcc ttcctcagc cacttactc cacaagcat gataaacac gacaagggc aattctcca gctgtggcg gccgggcct gccatgcaa aagtccaag tcttcgtcc agtgttatg caccatggc agtcgactc atggcgaag aagatcttc aggtctagg tcgaagagt cagacgcgg ccgagtcaa gccacctgc agctggact ccgcagggt gggtgtgtt tggagcaat ctgacggga cggcaggtc gttctaggc acaacttcc ttactgcat ttgactgac attgaaagg aaagtcttg cagaaagta gctttagcg aaattacaa gccctcaac ttaggagtt gccattaaa gttccaaca gagaacgtg ggtgcggtg gtgagcaag ccgaagcgg aggccctac ttgttgaag aggaaagcc ctcaccacc agtattttc gacacaaat cgcaaggag ggcgacaag gagggtggc agtgccgga ggcctcgta ttcggaatt cccttgaca cagtgcgtg gagaacgac agactgaca agggccgca gccaggact agtcctttc cggagcggg gcagagctt tccggatca actgaagaa ccggcgact atggggaga cacgggtcg agatccagc tttagttcc ttaattgag gcgcctcaa ggtgacgag agcggctct tgcgagagc ctaatgttg cgcgagagg ctcgtagga agcgtccct ggcttgctc gacaccctt tcctgcggc tcaacctcc gatcttccg cacgaggag gaagcttcc gtccctaac atcctcagc gagtgcttt cgccacctg gagcagcac gggctgcac actttgggg attttccga gttagcacg tccaaaaag agaatcagg caattgagg gaagacttc gattgtggc aaggagacg tcgctggat gacgatcaa tgtccgcat gacgtagct acgctttta aaggagtat ttgagggac cttcctgat ccgcttctt tgtagagat ctctatcaa gcgttcgtt cagacacaa aggatacgc aacaggaga ctacaattc gaagccctt caacatctc attcaactg ctgccgacg gccaacagg gacacgctc tatgctctg ctgggcttc ctcgccacg gttgcccaa aacgcagcc gacacgaaa gacgagtca ggcgaatgt gtgtctggt aacaaaatg gattccaat aacttggcc accgtcttc gctccaaac attctccat tgcatcaaa cctggaagt tcaaaagaa atagcctca gaccgtccc gaagataga atagacata ataaacgtg gtacgaact ctaatcgac cactacaaa caactttat accatttcg gctgaactt ctagacgag atttacgtt cacatgatg gattcacac cctgaagct ttagaccag cttttgaac aaaagggac gcattatcg ggggccgat gagtcagtc gaggaagtt gatagtgaa agctattca gctccctgg agtcctaca ggacccaca cccgagttg ccactcgag agtgaagaa acgaagaaa atgtactct cgggaggag tttttgcac gaacgtgct gccatgggg ggacccaac gtcgggatg aggataagg cataaggac cgcattcgc gagcggagt tcgaagaaa cgcagggat gaatcaacc tcgcggaag aagaaggaa gatgagggt agtataggt tcgggtatt tcgtcggca atgcacata atctcgcgt ttgcgaggg cagaaagac gacgactac cagtacagc aagaatcgg tcttcgtct ctggagagc aacagttcg gcgcacacc gacagcgat atcaaccgc gtgttgcgc ttggaggac gatcgccgc aagtcctcc ccttacatc ttggacaac agcggtgtc ataaccgcc agtctgacg atccccgtc cagacggca acgcaacca tttccactg aacttggac gacgacatt ccttacatc gaggacgcg gaaaacagc cggcaacaa ataacaatc ggcttggtg cggacgccg actgcgccc cctcgacga cggcaacga tcaatcagc ggaagcgat tcatctgtt gcttcaaac gtgcagcca atcacagga tcgatatcc ctgattcaa ggatacgac tcagctgtg ggctcctct gccacgtat tccagtcct gtccagaat acgtcgagc agcacggcg ggggacgcg gccgtccaa actgtcaat tacatgccg gaagagctg caaagaagt gcgaaaaag accccgagt gcggcgacg cttcaaaaa gtctcgttc tcctcaacg caggaagtg caacaaatc aaaccgaaa agttccaaa gagcaggat ttgcagaaa agtttcagt gtctcgact ttcgagcaa attaaaaca gaacccaaa catactcca agcatctcg agtattggg aatgcggtc ctaaggtcg cgaacggcc gattttgag aggatcacc aaagttgaa gtacaaaaa ccgaaagct agctcagaa aagaagaag tataccaaa cggaggtat acggattca aggcaccag accaggcat attcccgat tcggaaagt ctggaatcg gcgaatgca caaagtaaa aaggaagat acgacgatt gtggcacaa gcacctgtt tataaaaga agggaactc atctcgagc gttccatcc aagtag
- >TC032946 RA
agtgcatgt cgtataagc gtctgaaaa tagtcagaa gctcgtgtg ttttgcatt cattagaag tcattcttc aggtgcgcg tgttcacac agtgaattt gtttgttta acatgagga ggtgcacga gtttgttga aagtttaag tgcgatgtg aacctgtga cgatttcaa tgataaccg tcgcaagga gcgatgtgg aggtggcca ccagcgtct ccttcaagc actttacac tgatgggca tcgaaacca cctcgcaat acgtgcaaa agaccaaaa ctggcgctt ggtaggtgt ctcttgcca aatgggata tttttgttt ccaagtagc cgctacttg ctctgtcgc gtcagtgtt gagtggcta accaagtgg acttaaact gaccgcgtg actgcccaa aaaacccac aaaacgtac catgacagt ccaaccaac tcaacattc ggacccttt gacccaaat cggtgctaa atgcaagac gtgctaaat ttagttcgg tgtgacgat aagtgacac taataaacc agtaactca aaggttggc agctgaaac atgagagtt gaccctagc aaagccctt aaaacgtgc gccgaaaca atttaaata tttgcaagc ctacgctga aatctgaac gatgtttgc caagacaga ggtacagtt ttagattat aattcttta actatttta ttgtttatt aaaatattc ggacttgat cgatcggac acaataatt gaacgcttg ccaatatgt gaagacagg taaaattaa aaactatgt cgcaacttt gagctaaaa tggcggagc gttctcgga aaactttag gcaggagtg cctcaaccg aaagcaccg aatattcgc taacaatgg gtcgctcgg tccgcgaca agaaaaaga aaccaaagg agcgctggc ttctaacac gcaaaacct ggaggtaca tggcggacg ccggccgga aactcatcc cagatgggg cccaaaacc ggcccgaag acatcccca aaatcgagg cctacttcc aggaggtgt gcaaacggg agcccaagt tcctccttt ggcgcaaaa actcctatc ctggagcct tggccttcc ggaagaaag tccggaagg agcggcgca aagggggca gttgtcgga aagcctcct cagctgaca gtgtcgatc tgatcaagt tggaacgac caacagact tggccttac caagcacaa gcggcgctc gatacgaca tccagaaaa tgaaatatg attttttaa acaaaccgt ctccgacca gcttaaaca cacaggagg atttggagg aaaaacagc tgttgagta tgctggaac aatacttga cgatttcgg aacaaaccg cctcgacct cgaaataca atcctcccg attttaatt accaggaat tgatcgata agttgcaaa gacacttga ctcttgcaa ctaggagtt ttgtcaatg ttccgactt atcagagcc aaagtcagg tgcatttta acggcgatc atgtccaaa aatcactct cggagactt tgagtcgtt attttggcc aacacacga atcgggaca aagtcattt ctgatttac tcactgatc ggaaagctt tggaaaagc tttattttg atttgagga aagctcgcg gttttagga ggaatgtga gtggaagtg attattcat cagggatta gttttaaac cgaatttgt ccaaatatc cgatccaaa gttcgaatt atgagaaac tgaaggaac ggatgtatg agtctcacc cacctccct taatcgaaa ttgaaaatg agtctgcga gtgttagtt atcgcagtt gggggattc agactttac cagtccccg agtcagttc tcctggagg ttgaggaga agtacaaga aggctcagg ccgagaagg aggaggagg agaggaggg atttgagac ctgcaggtc gtagacgaa gcagtgtcg ataacgacg acatctccc aatccgtca gcgacacaa ttaagcggt atttacgga tggcgcgaa aaaagagcg tagacacgg acaaagtgg accgcttca aacgagtga attacgaca gaaacttgc ggaacatca aagcaaaag gagaaataa caaaaccag gagacgacg acggtctaa ataaaggtt gtcaaacca atgacgatt ggattttga aataccgcg atttgaaat tcgatgatt tttccgata ttgaaagcc ggctttcta gttcgcgaa gtagtgtgg atgagaaca ataaatcct cccctagct caccacctc cgaccaaat cccattcct tcctcagcc acttactcc acaagcatg ataaacacg acaagggca attctccag ctgtggcgg ccgggcctg ccatgcaaa agtccaagt cttcgtcca gtgttatgc accatggca gtcgactca tggcgaaga agatcttca ggtctaggt cgaagagtc agacgcggc cgagtcaag ccacctgca gctggactc cgcagggtg ggtgtgttt ggagcaatc tgacgggac ggcaggtcg ttctaggca caacttcct tactgcatt tgactgaca ttgaaagga aagtcttgc agaaagtag ctttagcga aattacaag ccctcaact taggagttg ccattaaag ttccaacag agaacgtgg gtgcggtgg tgagcaagc cgaagcgga ggccctact tgttgaaga ggaaagccc tcaccacca gtattttcg acacaaatc gcaaggagg gcgacaagg agggtggca gtgccggag gcctcgtat tcggaattc ccttgacac agtgcgtgg agaacgaca gactgacaa gggccgcag ccaggacta gtcctttcc ggagcgggg cagagcttt ccggatcaa ctgaagaac cggcgacta tggggagac acgggtcga gatccagct ttagttcct taattgagg cgcctcaag gtgacgaga gcggctctt gcgagagcc taatgttgc gcgagaggc tcgtaggaa gcgtccctg gcttgctcg acacccttt cctgcggct caacctccg atcttccgc acgaggagg aagcttccg tccctaaca tcctcagcg agtgctttc gccacctgg agcagcacg ggctgcaca ctttgggga ttttccgag ttagcacgt ccaaaaaga gaatcaggc aattgaggg aagacttcg attgtggca aggagacgt cgctggatg acgatcaat gtccgcatg acgtagcta cgcttttaa aggagtatt tgagggacc ttcctgatc cgcttcttt gtagagatc tctatcaag cgttcgttc agacacaaa ggatacgca acaggagac tacaattcg aagcccttc aacatctca ttcaactgc tgccgacgg ccaacaggg acacgctct atgctctgc tgggcttcc tcgccacgg ttgcccaaa acgcagccg acacgaaag acgagtcag gcgaatgtg tgtctggta acaaaatgg attccaata acttggcca ccgtcttcg ctccaaaca ttctccatt gcatcaaac ctggaagtt caaaagaaa tagcctcag accgtcccg aagatagaa tagacataa taaacgtgg tacgaactc taatcgacc actacaaac aactttata ccatttcgg ctgaacttc tagacgaga tttacgttc acatgatgg attcacacc ctgaagctt tagaccagc ttttgaaca aaagggacg cattatcgg gggccgatg agtcagtcg aggaagttg atagtgaaa gctattcag ctccctgga gtcctacag gacccacac ccgagttgc cactcgaga gtgaagaaa cgaagaaaa tgtactctc gggaggagt ttttgcacg aacgtgctg ccatggggg gacccaacg tcgggatga ggataaggc ataaggacc gcattcgcg agcggagtt cgaagaaac gcagggatg aatcaacct cgcggaaga agaaggaag atgagggta gtataggtt cgggtattt cgtcggcaa tgcacataa tctcgcgtt tgcgagggc agaaagacg acgactacc agtacagca agaatcggt cttcgtctc tggagagca acagttcgg cgcacaccg acagcgata tcaaccgcg tgttgcgct tggaggacg atcgccgca agtcctccc cttacatct tggacaaca gcggtgtca taaccgcca gtctgacga tccccgtcc agacggcaa cgcaaccat ttccactga acttggacg acgacattc cttacatcg aggacgcgg aaaacagcc ggcaacaaa taacaatcg gcttggtgc ggacgccga ctgcgcccc ctcgacgac ggcaacgat caatcagcg gaagcgatt catctgttg cttcaaacg tgcagccaa tcacaggat cgatatccc tgattcaag gatacgact cagctgtgg gctcctctg ccacgtatt ccagtcctg tccagaata cgtcgagca gcacggcgg gggacgcgg ccgtccaaa ctgtcaatt acatgccgg aagagctgc aaagaagtg cgaaaaaga ccccgagtg cggcgacgc ttcaaaaag tctcgttct cctcaacgc aggaagtgc aacaaatca aaccgaaaa gttccaaag agcaggatt tgcagaaaa gtttcagtg tctcgactt tcgagcaaa ttaaaacag aacccaaac atactccaa gcatctcga gtattggga atgcggtcc taaggtcgc gaacggccg attttgaga ggatcacca aagttgaag tacaaaaac cgaaagcta gctcagaaa agaagaagt ataccaaac ggaggtata cggattcaa ggcaccaga ccaggcata ttcccgatt cggaaagtc tggaatcgg cgaatgcac aaagtaaaa aggaagata cgacgattg tggcacaag cacctgttt ataaaagaa gggaactca tctcgagcg ttccatcca agtagcgga gacagggag atatctttc gcgaatgtg ttcaaaact tacaagtga tcagtgtct agctctagt cctatctag cgtttactg tcccaaaat tgttatcta ttttctaag gatgcatgc aaacgaaca gtgtgttgg tgtaaaaat taaaacggc ttaagtcaa taaataata acgtgataa
