Status
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Gene information
General information
TC032946
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008395,TC008396
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9664163..9679180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032946
    iBeetle screen iB_04479

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032946 RA
      MAERSRKTL GRSASTEST EYSLTMGRS VRDKKKKPK 
      ERWLLTRKT WRYMADAGR KLIPDGAQN RPEDIPKIE 
      AYFQEVCKR EPKFLLWRK NSYPGALAF RKKVRKERR 
      KGGSCRKAS SADSVDLIK LERPTDLAL PSTSGARYD 
      IQKMKYDFL NKPSPTSLN TQEDLEEKQ LLSMLEQYL 
      TISEQTAST SKYNPPDFN YQELIDKLQ RHLTLATRS 
      FVNVPTYQS QSQVHFNGD HVQKSLSET LSRYFGQHT 
      NRDKVISDL LTDRKALEK LYFDLRKAR GFRRNVSGS 
      DYSSGISFK PNLSKYPIQ SSNYEKLKE RMYESHPPP 
      LIEIENESA SVSYRSWGI QTLPVPESV LLEVEEKYK 
      KAQAEKEEE ERRDLRPAG RRRSSVDND DISQSVSDT 
      IKRYLRMAR KKSVDTDKV DRFKRVNYD RNLRNIKAK 
      GEITKPGDD DGLNKGCQT NDDWILKYR DLKFDDFSD 
      IESRLSSSR SSVDENNKS SPSSPPPTK SHSFLSHLL 
      HKHDKHDKG NSPAVAAGP AMQKSKSSS SVMHHGSRL 
      MAKKIFRSR SKSQTRPSQ ATCSWTPQG GCVWSNLTG 
      RQVVLGTTS LLHLTDIER KVLQKVALA KLQALNLGV 
      AIKVPTENV GAVVSKPKR RPYLLKRKA LTTSIFDTN 
      RKEGDKEGG SAGGLVFGI PLTQCVEND RLTRAAART 
      SPFRSGAEL SGSTEEPAT MGRHGSRSS FSSLIEAPQ 
      GDESGSCES LMLRERLVG SVPGLLDTL SCGSTSDLP 
      HEEEASVPN ILSECFRHL EQHGLHTLG IFRVSTSKK 
      RIRQLREDF DCGKETSLD DDQCPHDVA TLLKEYLRD 
      LPDPLLCRD LYQAFVQTQ RIRNRRLQF EALQHLIQL 
      LPTANRDTL YALLGFLAT VAQNAADTK DESGECVSG 
      NKMDSNNLA TVFAPNILH CIKPGSSKE IASDRPEDR 
      IDIINVVRT LIDHYKQLY TISAELLDE IYVHMMDSH 
      PEALDQLLN KRDALSGAD ESVEEVDSE SYSAPWSPT 
      GPTPELPLE SEETKKMYS REEFLHERA AMGGPNVGM 
      RIRHKDRIR ERSSKKRRD ESTSRKKKE DEGSIGSGI 
      SSAMHIISR LRGQKDDDY QYSKNRSSS LESNSSAHT 
      DSDINRVLR LEDDRRKSS PYILDNSGV ITASLTIPV 
      QTATQPFPL NLDDDIPYI EDAENSRQQ ITIGLVRTP 
      TAPPRRRQR SISGSDSSV ASNVQPITG SISLIQGYD 
      SAVGSSATY SSPVQNTSS STAGDAAVQ TVNYMPEEL 
      QRSAKKTPS AATLQKVSF SSTQEVQQI KPKSSKEQD 
      LQKSFSVST FEQIKTEPK HTPSISSIG NAVLRSRTA 
      DFERITKVE VQKPKASSE KKKYTKRRY TDSRHQTRH 
      IPDSESLES ANAQSKKED TTIVAQAPV YKRRELISS 
      VPSK
    CDS sequence
    • >TC032946 RA
      atggcggag cgttctcgg aaaacttta ggcaggagt 
      gcctcaacc gaaagcacc gaatattcg ctaacaatg 
      ggtcgctcg gtccgcgac aagaaaaag aaaccaaag 
      gagcgctgg cttctaaca cgcaaaacc tggaggtac 
      atggcggac gccggccgg aaactcatc ccagatggg 
      gcccaaaac cggcccgaa gacatcccc aaaatcgag 
      gcctacttc caggaggtg tgcaaacgg gagcccaag 
      ttcctcctt tggcgcaaa aactcctat cctggagcc 
      ttggccttc cggaagaaa gtccggaag gagcggcgc 
      aaagggggc agttgtcgg aaagcctcc tcagctgac 
      agtgtcgat ctgatcaag ttggaacga ccaacagac 
      ttggcctta ccaagcaca agcggcgct cgatacgac 
      atccagaaa atgaaatat gatttttta aacaaaccg 
      tctccgacc agcttaaac acacaggag gatttggag 
      gaaaaacag ctgttgagt atgctggaa caatacttg 
      acgatttcg gaacaaacc gcctcgacc tcgaaatac 
      aatcctccc gattttaat taccaggaa ttgatcgat 
      aagttgcaa agacacttg actcttgca actaggagt 
      tttgtcaat gttccgact tatcagagc caaagtcag 
      gtgcatttt aacggcgat catgtccaa aaatcactc 
      tcggagact ttgagtcgt tattttggc caacacacg 
      aatcgggac aaagtcatt tctgattta ctcactgat 
      cggaaagct ttggaaaag ctttatttt gatttgagg 
      aaagctcgc ggttttagg aggaatgtg agtggaagt 
      gattattca tcagggatt agttttaaa ccgaatttg 
      tccaaatat ccgatccaa agttcgaat tatgagaaa 
      ctgaaggaa cggatgtat gagtctcac ccacctccc 
      ttaatcgaa attgaaaat gagtctgcg agtgttagt 
      tatcgcagt tgggggatt cagacttta ccagtcccc 
      gagtcagtt ctcctggag gttgaggag aagtacaag 
      aaggctcag gccgagaag gaggaggag gagaggagg 
      gatttgaga cctgcaggt cgtagacga agcagtgtc 
      gataacgac gacatctcc caatccgtc agcgacaca 
      attaagcgg tatttacgg atggcgcga aaaaagagc 
      gtagacacg gacaaagtg gaccgcttc aaacgagtg 
      aattacgac agaaacttg cggaacatc aaagcaaaa 
      ggagaaata acaaaacca ggagacgac gacggtcta 
      aataaaggt tgtcaaacc aatgacgat tggattttg 
      aaataccgc gatttgaaa ttcgatgat ttttccgat 
      attgaaagc cggctttct agttcgcga agtagtgtg 
      gatgagaac aataaatcc tcccctagc tcaccacct 
      ccgaccaaa tcccattcc ttcctcagc cacttactc 
      cacaagcat gataaacac gacaagggc aattctcca 
      gctgtggcg gccgggcct gccatgcaa aagtccaag 
      tcttcgtcc agtgttatg caccatggc agtcgactc 
      atggcgaag aagatcttc aggtctagg tcgaagagt 
      cagacgcgg ccgagtcaa gccacctgc agctggact 
      ccgcagggt gggtgtgtt tggagcaat ctgacggga 
      cggcaggtc gttctaggc acaacttcc ttactgcat 
      ttgactgac attgaaagg aaagtcttg cagaaagta 
      gctttagcg aaattacaa gccctcaac ttaggagtt 
      gccattaaa gttccaaca gagaacgtg ggtgcggtg 
      gtgagcaag ccgaagcgg aggccctac ttgttgaag 
      aggaaagcc ctcaccacc agtattttc gacacaaat 
      cgcaaggag ggcgacaag gagggtggc agtgccgga 
      ggcctcgta ttcggaatt cccttgaca cagtgcgtg 
      gagaacgac agactgaca agggccgca gccaggact 
      agtcctttc cggagcggg gcagagctt tccggatca 
      actgaagaa ccggcgact atggggaga cacgggtcg 
      agatccagc tttagttcc ttaattgag gcgcctcaa 
      ggtgacgag agcggctct tgcgagagc ctaatgttg 
      cgcgagagg ctcgtagga agcgtccct ggcttgctc 
      gacaccctt tcctgcggc tcaacctcc gatcttccg 
      cacgaggag gaagcttcc gtccctaac atcctcagc 
      gagtgcttt cgccacctg gagcagcac gggctgcac 
      actttgggg attttccga gttagcacg tccaaaaag 
      agaatcagg caattgagg gaagacttc gattgtggc 
      aaggagacg tcgctggat gacgatcaa tgtccgcat 
      gacgtagct acgctttta aaggagtat ttgagggac 
      cttcctgat ccgcttctt tgtagagat ctctatcaa 
      gcgttcgtt cagacacaa aggatacgc aacaggaga 
      ctacaattc gaagccctt caacatctc attcaactg 
      ctgccgacg gccaacagg gacacgctc tatgctctg 
      ctgggcttc ctcgccacg gttgcccaa aacgcagcc 
      gacacgaaa gacgagtca ggcgaatgt gtgtctggt 
      aacaaaatg gattccaat aacttggcc accgtcttc 
      gctccaaac attctccat tgcatcaaa cctggaagt 
      tcaaaagaa atagcctca gaccgtccc gaagataga 
      atagacata ataaacgtg gtacgaact ctaatcgac 
      cactacaaa caactttat accatttcg gctgaactt 
      ctagacgag atttacgtt cacatgatg gattcacac 
      cctgaagct ttagaccag cttttgaac aaaagggac 
      gcattatcg ggggccgat gagtcagtc gaggaagtt 
      gatagtgaa agctattca gctccctgg agtcctaca 
      ggacccaca cccgagttg ccactcgag agtgaagaa 
      acgaagaaa atgtactct cgggaggag tttttgcac 
      gaacgtgct gccatgggg ggacccaac gtcgggatg 
      aggataagg cataaggac cgcattcgc gagcggagt 
      tcgaagaaa cgcagggat gaatcaacc tcgcggaag 
      aagaaggaa gatgagggt agtataggt tcgggtatt 
      tcgtcggca atgcacata atctcgcgt ttgcgaggg 
      cagaaagac gacgactac cagtacagc aagaatcgg 
      tcttcgtct ctggagagc aacagttcg gcgcacacc 
      gacagcgat atcaaccgc gtgttgcgc ttggaggac 
      gatcgccgc aagtcctcc ccttacatc ttggacaac 
      agcggtgtc ataaccgcc agtctgacg atccccgtc 
      cagacggca acgcaacca tttccactg aacttggac 
      gacgacatt ccttacatc gaggacgcg gaaaacagc 
      cggcaacaa ataacaatc ggcttggtg cggacgccg 
      actgcgccc cctcgacga cggcaacga tcaatcagc 
      ggaagcgat tcatctgtt gcttcaaac gtgcagcca 
      atcacagga tcgatatcc ctgattcaa ggatacgac 
      tcagctgtg ggctcctct gccacgtat tccagtcct 
      gtccagaat acgtcgagc agcacggcg ggggacgcg 
      gccgtccaa actgtcaat tacatgccg gaagagctg 
      caaagaagt gcgaaaaag accccgagt gcggcgacg 
      cttcaaaaa gtctcgttc tcctcaacg caggaagtg 
      caacaaatc aaaccgaaa agttccaaa gagcaggat 
      ttgcagaaa agtttcagt gtctcgact ttcgagcaa 
      attaaaaca gaacccaaa catactcca agcatctcg 
      agtattggg aatgcggtc ctaaggtcg cgaacggcc 
      gattttgag aggatcacc aaagttgaa gtacaaaaa 
      ccgaaagct agctcagaa aagaagaag tataccaaa 
      cggaggtat acggattca aggcaccag accaggcat 
      attcccgat tcggaaagt ctggaatcg gcgaatgca 
      caaagtaaa aaggaagat acgacgatt gtggcacaa 
      gcacctgtt tataaaaga agggaactc atctcgagc 
      gttccatcc aagtag
    mRNA sequence
    • >TC032946 RA
      agtgcatgt cgtataagc gtctgaaaa tagtcagaa 
      gctcgtgtg ttttgcatt cattagaag tcattcttc 
      aggtgcgcg tgttcacac agtgaattt gtttgttta 
      acatgagga ggtgcacga gtttgttga aagtttaag 
      tgcgatgtg aacctgtga cgatttcaa tgataaccg 
      tcgcaagga gcgatgtgg aggtggcca ccagcgtct 
      ccttcaagc actttacac tgatgggca tcgaaacca 
      cctcgcaat acgtgcaaa agaccaaaa ctggcgctt 
      ggtaggtgt ctcttgcca aatgggata tttttgttt 
      ccaagtagc cgctacttg ctctgtcgc gtcagtgtt 
      gagtggcta accaagtgg acttaaact gaccgcgtg 
      actgcccaa aaaacccac aaaacgtac catgacagt 
      ccaaccaac tcaacattc ggacccttt gacccaaat 
      cggtgctaa atgcaagac gtgctaaat ttagttcgg 
      tgtgacgat aagtgacac taataaacc agtaactca 
      aaggttggc agctgaaac atgagagtt gaccctagc 
      aaagccctt aaaacgtgc gccgaaaca atttaaata 
      tttgcaagc ctacgctga aatctgaac gatgtttgc 
      caagacaga ggtacagtt ttagattat aattcttta 
      actatttta ttgtttatt aaaatattc ggacttgat 
      cgatcggac acaataatt gaacgcttg ccaatatgt 
      gaagacagg taaaattaa aaactatgt cgcaacttt 
      gagctaaaa tggcggagc gttctcgga aaactttag 
      gcaggagtg cctcaaccg aaagcaccg aatattcgc 
      taacaatgg gtcgctcgg tccgcgaca agaaaaaga 
      aaccaaagg agcgctggc ttctaacac gcaaaacct 
      ggaggtaca tggcggacg ccggccgga aactcatcc 
      cagatgggg cccaaaacc ggcccgaag acatcccca 
      aaatcgagg cctacttcc aggaggtgt gcaaacggg 
      agcccaagt tcctccttt ggcgcaaaa actcctatc 
      ctggagcct tggccttcc ggaagaaag tccggaagg 
      agcggcgca aagggggca gttgtcgga aagcctcct 
      cagctgaca gtgtcgatc tgatcaagt tggaacgac 
      caacagact tggccttac caagcacaa gcggcgctc 
      gatacgaca tccagaaaa tgaaatatg attttttaa 
      acaaaccgt ctccgacca gcttaaaca cacaggagg 
      atttggagg aaaaacagc tgttgagta tgctggaac 
      aatacttga cgatttcgg aacaaaccg cctcgacct 
      cgaaataca atcctcccg attttaatt accaggaat 
      tgatcgata agttgcaaa gacacttga ctcttgcaa 
      ctaggagtt ttgtcaatg ttccgactt atcagagcc 
      aaagtcagg tgcatttta acggcgatc atgtccaaa 
      aatcactct cggagactt tgagtcgtt attttggcc 
      aacacacga atcgggaca aagtcattt ctgatttac 
      tcactgatc ggaaagctt tggaaaagc tttattttg 
      atttgagga aagctcgcg gttttagga ggaatgtga 
      gtggaagtg attattcat cagggatta gttttaaac 
      cgaatttgt ccaaatatc cgatccaaa gttcgaatt 
      atgagaaac tgaaggaac ggatgtatg agtctcacc 
      cacctccct taatcgaaa ttgaaaatg agtctgcga 
      gtgttagtt atcgcagtt gggggattc agactttac 
      cagtccccg agtcagttc tcctggagg ttgaggaga 
      agtacaaga aggctcagg ccgagaagg aggaggagg 
      agaggaggg atttgagac ctgcaggtc gtagacgaa 
      gcagtgtcg ataacgacg acatctccc aatccgtca 
      gcgacacaa ttaagcggt atttacgga tggcgcgaa 
      aaaagagcg tagacacgg acaaagtgg accgcttca 
      aacgagtga attacgaca gaaacttgc ggaacatca 
      aagcaaaag gagaaataa caaaaccag gagacgacg 
      acggtctaa ataaaggtt gtcaaacca atgacgatt 
      ggattttga aataccgcg atttgaaat tcgatgatt 
      tttccgata ttgaaagcc ggctttcta gttcgcgaa 
      gtagtgtgg atgagaaca ataaatcct cccctagct 
      caccacctc cgaccaaat cccattcct tcctcagcc 
      acttactcc acaagcatg ataaacacg acaagggca 
      attctccag ctgtggcgg ccgggcctg ccatgcaaa 
      agtccaagt cttcgtcca gtgttatgc accatggca 
      gtcgactca tggcgaaga agatcttca ggtctaggt 
      cgaagagtc agacgcggc cgagtcaag ccacctgca 
      gctggactc cgcagggtg ggtgtgttt ggagcaatc 
      tgacgggac ggcaggtcg ttctaggca caacttcct 
      tactgcatt tgactgaca ttgaaagga aagtcttgc 
      agaaagtag ctttagcga aattacaag ccctcaact 
      taggagttg ccattaaag ttccaacag agaacgtgg 
      gtgcggtgg tgagcaagc cgaagcgga ggccctact 
      tgttgaaga ggaaagccc tcaccacca gtattttcg 
      acacaaatc gcaaggagg gcgacaagg agggtggca 
      gtgccggag gcctcgtat tcggaattc ccttgacac 
      agtgcgtgg agaacgaca gactgacaa gggccgcag 
      ccaggacta gtcctttcc ggagcgggg cagagcttt 
      ccggatcaa ctgaagaac cggcgacta tggggagac 
      acgggtcga gatccagct ttagttcct taattgagg 
      cgcctcaag gtgacgaga gcggctctt gcgagagcc 
      taatgttgc gcgagaggc tcgtaggaa gcgtccctg 
      gcttgctcg acacccttt cctgcggct caacctccg 
      atcttccgc acgaggagg aagcttccg tccctaaca 
      tcctcagcg agtgctttc gccacctgg agcagcacg 
      ggctgcaca ctttgggga ttttccgag ttagcacgt 
      ccaaaaaga gaatcaggc aattgaggg aagacttcg 
      attgtggca aggagacgt cgctggatg acgatcaat 
      gtccgcatg acgtagcta cgcttttaa aggagtatt 
      tgagggacc ttcctgatc cgcttcttt gtagagatc 
      tctatcaag cgttcgttc agacacaaa ggatacgca 
      acaggagac tacaattcg aagcccttc aacatctca 
      ttcaactgc tgccgacgg ccaacaggg acacgctct 
      atgctctgc tgggcttcc tcgccacgg ttgcccaaa 
      acgcagccg acacgaaag acgagtcag gcgaatgtg 
      tgtctggta acaaaatgg attccaata acttggcca 
      ccgtcttcg ctccaaaca ttctccatt gcatcaaac 
      ctggaagtt caaaagaaa tagcctcag accgtcccg 
      aagatagaa tagacataa taaacgtgg tacgaactc 
      taatcgacc actacaaac aactttata ccatttcgg 
      ctgaacttc tagacgaga tttacgttc acatgatgg 
      attcacacc ctgaagctt tagaccagc ttttgaaca 
      aaagggacg cattatcgg gggccgatg agtcagtcg 
      aggaagttg atagtgaaa gctattcag ctccctgga 
      gtcctacag gacccacac ccgagttgc cactcgaga 
      gtgaagaaa cgaagaaaa tgtactctc gggaggagt 
      ttttgcacg aacgtgctg ccatggggg gacccaacg 
      tcgggatga ggataaggc ataaggacc gcattcgcg 
      agcggagtt cgaagaaac gcagggatg aatcaacct 
      cgcggaaga agaaggaag atgagggta gtataggtt 
      cgggtattt cgtcggcaa tgcacataa tctcgcgtt 
      tgcgagggc agaaagacg acgactacc agtacagca 
      agaatcggt cttcgtctc tggagagca acagttcgg 
      cgcacaccg acagcgata tcaaccgcg tgttgcgct 
      tggaggacg atcgccgca agtcctccc cttacatct 
      tggacaaca gcggtgtca taaccgcca gtctgacga 
      tccccgtcc agacggcaa cgcaaccat ttccactga 
      acttggacg acgacattc cttacatcg aggacgcgg 
      aaaacagcc ggcaacaaa taacaatcg gcttggtgc 
      ggacgccga ctgcgcccc ctcgacgac ggcaacgat 
      caatcagcg gaagcgatt catctgttg cttcaaacg 
      tgcagccaa tcacaggat cgatatccc tgattcaag 
      gatacgact cagctgtgg gctcctctg ccacgtatt 
      ccagtcctg tccagaata cgtcgagca gcacggcgg 
      gggacgcgg ccgtccaaa ctgtcaatt acatgccgg 
      aagagctgc aaagaagtg cgaaaaaga ccccgagtg 
      cggcgacgc ttcaaaaag tctcgttct cctcaacgc 
      aggaagtgc aacaaatca aaccgaaaa gttccaaag 
      agcaggatt tgcagaaaa gtttcagtg tctcgactt 
      tcgagcaaa ttaaaacag aacccaaac atactccaa 
      gcatctcga gtattggga atgcggtcc taaggtcgc 
      gaacggccg attttgaga ggatcacca aagttgaag 
      tacaaaaac cgaaagcta gctcagaaa agaagaagt 
      ataccaaac ggaggtata cggattcaa ggcaccaga 
      ccaggcata ttcccgatt cggaaagtc tggaatcgg 
      cgaatgcac aaagtaaaa aggaagata cgacgattg 
      tggcacaag cacctgttt ataaaagaa gggaactca 
      tctcgagcg ttccatcca agtagcgga gacagggag 
      atatctttc gcgaatgtg ttcaaaact tacaagtga 
      tcagtgtct agctctagt cctatctag cgtttactg 
      tcccaaaat tgttatcta ttttctaag gatgcatgc 
      aaacgaaca gtgtgttgg tgtaaaaat taaaacggc 
      ttaagtcaa taaataata acgtgataa
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