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Gene information
General information
TC008388 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9633463..9640937
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008388
    iBeetle screen iB_04478

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008388 RA
      MEDLQTDTV TDYGKKQNP GNPDFSKDS ENDCGENLL 
      NGSDELDPL GLMDQCDES IGSNSDVWK GSDSEDDSF 
      EKEKAQTSQ IENVAEEKL GETSVPTEQ NEEKGETEA 
      PETVQNVIE SEGTDSAEL PKDQEEAEK ERETEKLDC 
      KTDDPLSSK DHEEEKIGK LDCKTGDAD VSKDQVEEK 
      IEKLDCKTG DTDVSKDQE EAENDKTGN VDENSKDQA 
      EVENKPENE KLDSSADVT ATKSPEVPV GTEKANEKE 
      KDSLVPQTS ENQPESSQG PLAEPTQNS SKDTTPNCV 
      AVDQDEDCH MDYDAEDDD DFDPSLLCP EVSMDVDEN 
      RVITNNGTV PNESGSGSP LPYEPLFSA FVDDVTGAE 
      IAFNLTPEE LELKKRLYG ATNPIQYTR IHCTACNVH 
      LGSALEGQN NRFVHPLLK VLICKECYH FYTSGEFEK 
      DEDGSELYC RWCGQGGQV LCCSKCEFV FCKRCIRTN 
      FGRKKMFEI RDSDSWECF RCNSSQIIT QRIACYEFF 
      EYIRREMAW AANANNQEL WSKDYSRCC GGDKKRSAV 
      GEEKKAKRK KSSDPDYNP FVDYSKMSE PKTDPLAFG 
      GNKVTLPKL VQIVPKVNS VGDKTKDND IEFVRQIPA 
      PHVTINPTN RPAYRTILP AVRPGLQPS TYVRLNPAS 
      PLAPTTNRF VSTAINNRN LMKHEWFEK TVRAAARVN 
      SHLSYTLTQ LNKAQSQAG SVEELAVVH NKLQEILSS 
      SINSLIQIR KNLRTEFIA GIKTVHLPS KKPEQSDDD 
      VIIVSPPAV MPKPAPCPT KTAIDPLII IEKPKTNST 
      KTVTTGPQK GFLKVKSVS ELQNIPPEC ITIPDDPPT 
      VTNKPCNGG SPKVETYKV ADKSVDVKK MMAVRVNLI 
      RDDLEKLQR QMKKNS
    CDS sequence
    • >TC008388 RA
      atggaggat ctgcagacg gatacagtg accgattat 
      ggaaaaaaa caaaatccg ggaaaccca gatttcagt 
      aaagacagt gaaaatgac tgtggtgag aatctcctc 
      aacggaagc gacgaactt gatccttta ggtctcatg 
      gatcagtgt gatgagagc attggcagt aacagtgat 
      gtctggaaa ggaagtgac agtgaggat gacagtttt 
      gaaaaggaa aaggcacaa actagtcag attgaaaac 
      gtggctgaa gaaaaattg ggtgaaacc tcagtccct 
      actgaacag aatgaagaa aaaggtgaa actgaagcg 
      cctgaaacg gtacaaaac gtgattgaa agtgaagga 
      acagacagt gctgagctc ccaaaagac caagaggaa 
      gcagagaag gaacgagaa actgaaaaa cttgactgt 
      aaaactgat gatcctctc agttcgaaa gatcacgag 
      gaagaaaag attggaaaa cttgactgt aaaactggt 
      gatgctgat gtctcaaaa gatcaagtg gaagaaaag 
      attgaaaaa cttgactgt aagactggt gatactgat 
      gtctcaaaa gatcaagag gaagcagag aatgataaa 
      accggcaat gttgatgag aactcaaaa gatcaggcg 
      gaagtagag aataagcct gagaacgag aaacttgac 
      tcttctgct gacgtcaca gccacaaag tcaccagaa 
      gttcctgtt ggcactgag aaagctaat gaaaaagag 
      aaagattct ttagtccca caaacttcc gaaaaccaa 
      ccagaatca agtcaaggc ccccttgct gaacccaca 
      caaaattct tcaaaagat acaactccc aactgcgta 
      gcagtcgac caagatgaa gattgccac atggattac 
      gacgctgaa gatgacgat gattttgac ccatcattg 
      ctctgtcct gaagtatca atggacgtt gatgagaat 
      cgtgtcatc actaacaat ggtacagtg ccaaatgag 
      tcaggttca ggtagtcca ttgccatac gaaccgctt 
      ttttccgca tttgttgac gacgtgaca ggagctgaa 
      attgcgttt aatttaacc cctgaggaa ctcgaattg 
      aagaaaaga ctgtacgga gctaccaat ccaatccaa 
      tacacacga attcattgc acggcttgc aatgtccac 
      ttagggagt gctttggag ggccaaaac aaccgattc 
      gtccaccca ttgttgaaa gttttaatt tgtaaagaa 
      tgttaccac ttttacacg agcggtgag ttcgaaaaa 
      gacgaagat ggaagcgag ctctactgc aggtggtgt 
      gggcaagga ggccaagtt ctgtgttgc tcgaaatgc 
      gaatttgtc ttttgcaaa agatgtatt cgaacgaat 
      tttgggcgc aagaaaatg ttcgaaatt cgcgacagc 
      gatagttgg gagtgtttc cgttgcaat tcgtcgcaa 
      atcatcact caacgaatc gcttgttat gaatttttt 
      gaatacatt cgtcgcgaa atggcatgg gctgctaat 
      gcaaataat caggagctg tggagtaag gattattcc 
      cgttgttgt ggcggggat aaaaaacga tcagcggtg 
      ggagaagag aagaaagcc aaaaggaaa aaatcgtca 
      gatcccgat tataatcct tttgtggat tacagtaaa 
      atgagtgaa cctaaaact gatcctctt gcctttgga 
      ggaaataaa gtcactctt cctaaacta gttcaaatc 
      gtgcctaaa gttaactca gtcggggat aaaacgaaa 
      gataacgat atcgagttt gtgaggcaa attccagcg 
      cctcatgtt actatcaac ccaacaaac agaccagcg 
      tatcggacg atattaccg gctgttagg ccagggctc 
      cagccttca acttacgtt cgcttaaac ccggcgtcc 
      cctctggcc cccacaaca aaccgtttc gtttccaca 
      gctatcaat aaccgaaat cttatgaaa cacgaatgg 
      tttgaaaag actgtgcga gcagcggcg cgcgtcaac 
      tcccactta tcctacact ttaactcag ttaaataaa 
      gcccaatcg caggcagga agtgttgaa gaactcgca 
      gtggttcat aacaagttg caagaaatt ctcagtagt 
      tctatcaat tcgctcatt caaatccgg aaaaatcta 
      cgaacggag tttatcgcc ggcattaaa accgttcat 
      ttgccctcg aaaaagccc gaacagagt gacgacgat 
      gtgattatt gttagtccc ccagcggta atgccaaaa 
      ccggcccct tgtcctacg aaaactgcc atcgatccg 
      ctaataatc atcgaaaaa cccaaaact aatagtaca 
      aaaacggtc accacaggg ccccagaag ggcttcctg 
      aaggtaaaa tcagtgtca gagttgcaa aacatccca 
      ccagagtgt ataacaatc cctgatgac ccgcctaca 
      gtgaccaat aaaccgtgc aacgggggt tcccccaaa 
      gtagaaacg tacaaggtg gcggataaa tcggttgac 
      gtcaaaaaa atgatggca gttagggtc aatttaatc 
      agggacgac ttagagaaa ttacaaaga cagatgaaa 
      aagaacagt taa
    mRNA sequence
    • >TC008388 RA
      agcgccaac cagctggtg aaaagcgaa actatttac 
      cgccgaaac agtaacaaa gttctcgaa gttctcgcg 
      cccctttgt gacaattta attcattcc gaacctgcg 
      taaaataat ttcgactta tcgtcaaac tttattcgt 
      ttgaaaata attgcgggt tttcgcgcg tcatcgtca 
      tcagttgag ttttttcag cagaatgcg gggattcgg 
      gtgatttta acaaattca ctcgatatt tttcccaaa 
      ttgagctgc aagaagtgg aactaacct caaatttgt 
      gacaatgga ggatctgca gacggatac agtgaccga 
      ttatggaaa aaaacaaaa tccgggaaa cccagattt 
      cagtaaaga cagtgaaaa tgactgtgg tgagaatct 
      cctcaacgg aagcgacga acttgatcc tttaggtct 
      catggatca gtgtgatga gagcattgg cagtaacag 
      tgatgtctg gaaaggaag tgacagtga ggatgacag 
      ttttgaaaa ggaaaaggc acaaactag tcagattga 
      aaacgtggc tgaagaaaa attgggtga aacctcagt 
      ccctactga acagaatga agaaaaagg tgaaactga 
      agcgcctga aacggtaca aaacgtgat tgaaagtga 
      aggaacaga cagtgctga gctcccaaa agaccaaga 
      ggaagcaga gaaggaacg agaaactga aaaacttga 
      ctgtaaaac tgatgatcc tctcagttc gaaagatca 
      cgaggaaga aaagattgg aaaacttga ctgtaaaac 
      tggtgatgc tgatgtctc aaaagatca agtggaaga 
      aaagattga aaaacttga ctgtaagac tggtgatac 
      tgatgtctc aaaagatca agaggaagc agagaatga 
      taaaaccgg caatgttga tgagaactc aaaagatca 
      ggcggaagt agagaataa gcctgagaa cgagaaact 
      tgactcttc tgctgacgt cacagccac aaagtcacc 
      agaagttcc tgttggcac tgagaaagc taatgaaaa 
      agagaaaga ttctttagt cccacaaac ttccgaaaa 
      ccaaccaga atcaagtca aggccccct tgctgaacc 
      cacacaaaa ttcttcaaa agatacaac tcccaactg 
      cgtagcagt cgaccaaga tgaagattg ccacatgga 
      ttacgacgc tgaagatga cgatgattt tgacccatc 
      attgctctg tcctgaagt atcaatgga cgttgatga 
      gaatcgtgt catcactaa caatggtac agtgccaaa 
      tgagtcagg ttcaggtag tccattgcc atacgaacc 
      gcttttttc cgcatttgt tgacgacgt gacaggagc 
      tgaaattgc gtttaattt aacccctga ggaactcga 
      attgaagaa aagactgta cggagctac caatccaat 
      ccaatacac acgaattca ttgcacggc ttgcaatgt 
      ccacttagg gagtgcttt ggagggcca aaacaaccg 
      attcgtcca cccattgtt gaaagtttt aatttgtaa 
      agaatgtta ccactttta cacgagcgg tgagttcga 
      aaaagacga agatggaag cgagctcta ctgcaggtg 
      gtgtgggca aggaggcca agttctgtg ttgctcgaa 
      atgcgaatt tgtcttttg caaaagatg tattcgaac 
      gaattttgg gcgcaagaa aatgttcga aattcgcga 
      cagcgatag ttgggagtg tttccgttg caattcgtc 
      gcaaatcat cactcaacg aatcgcttg ttatgaatt 
      ttttgaata cattcgtcg cgaaatggc atgggctgc 
      taatgcaaa taatcagga gctgtggag taaggatta 
      ttcccgttg ttgtggcgg ggataaaaa acgatcagc 
      ggtgggaga agagaagaa agccaaaag gaaaaaatc 
      gtcagatcc cgattataa tccttttgt ggattacag 
      taaaatgag tgaacctaa aactgatcc tcttgcctt 
      tggaggaaa taaagtcac tcttcctaa actagttca 
      aatcgtgcc taaagttaa ctcagtcgg ggataaaac 
      gaaagataa cgatatcga gtttgtgag gcaaattcc 
      agcgcctca tgttactat caacccaac aaacagacc 
      agcgtatcg gacgatatt accggctgt taggccagg 
      gctccagcc ttcaactta cgttcgctt aaacccggc 
      gtcccctct ggcccccac aacaaaccg tttcgtttc 
      cacagctat caataaccg aaatcttat gaaacacga 
      atggtttga aaagactgt gcgagcagc ggcgcgcgt 
      caactccca cttatccta cactttaac tcagttaaa 
      taaagccca atcgcaggc aggaagtgt tgaagaact 
      cgcagtggt tcataacaa gttgcaaga aattctcag 
      tagttctat caattcgct cattcaaat ccggaaaaa 
      tctacgaac ggagtttat cgccggcat taaaaccgt 
      tcatttgcc ctcgaaaaa gcccgaaca gagtgacga 
      cgatgtgat tattgttag tcccccagc ggtaatgcc 
      aaaaccggc cccttgtcc tacgaaaac tgccatcga 
      tccgctaat aatcatcga aaaacccaa aactaatag 
      tacaaaaac ggtcaccac agggcccca gaagggctt 
      cctgaaggt aaaatcagt gtcagagtt gcaaaacat 
      cccaccaga gtgtataac aatccctga tgacccgcc 
      tacagtgac caataaacc gtgcaacgg gggttcccc 
      caaagtaga aacgtacaa ggtggcgga taaatcggt 
      tgacgtcaa aaaaatgat ggcagttag ggtcaattt 
      aatcaggga cgacttaga gaaattaca aagacagat 
      gaaaaagaa cagttaatg tgtgaagtg atgggactt 
      ttttgtact ttctcttaa tattatatt gtctatatg 
      tgtcataag ctacatcaa agcggtgct ttcacgtag 
      cttaagttt ttgttaaat aaagcaacg tttttgttc 
      cgtgtgggt cccctaatt ttgtaccac ttgaggaca 
      gtattttgt tggattcgt acccacata tttaaatta 
      ttattctat tgtactgta aataaatct tgaaccaaa 
      ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY