Status
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Gene information
General information
TC032949
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008387,TC008385,TC008386
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9610743..9626268
Sequence information
Transcripts / Proteins (5) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032949
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04477

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032949 RA
      MVRKNRYQQ YSEPLPERG PVESLIYWR 
      DPKKSGPVF GGVLLVLLA LTYFSLISV 
      VAYLSLLGL TGTISFRIY KNIVQAVQK 
      TGDGHPFKD LLEFDVSLP QEKVKEVSE 
      VAVAHINAA VVELRRLFL VEDLVDSIK 
      FGVLLWTLT YLGAWFNGM TLIIIAWVA 
      LFSLPKVYE TNKTQIDAN LEIVRTKLA 
      EITSKVKAA IPIGKKAEE KKDQ
    • >TC032949 RB
      MAEDIKNAA NALVDDVLN GVSNEMNKT RGGADDKSR 
      QRSLLCVLF TPNTWFNPE RLNPKVESL IYWRDPKKS 
      GPVFGGVLL VLLALTYFS LISVVAYLS LLGLTGTIS 
      FRIYKNIVQ AVQKTGDGH PFKDLLEFD VSLPQEKVK 
      EVSEVAVAH INAAVVELR RLFLVEDLV DSIKFGVLL 
      WTLTYLGAW FNGMTLIII AWVALFSLP KVYETNKTQ 
      IDANLEIVR TKLAEITSK VKAAIPIGK KAEEKKDQ
    • >TC032949 RC
      MDNKELSLE GVRDALRQS HSQPVGGLR REVDSLDDF 
      EHLGHDSSP LKDGGDLLS ARDQPVINK GDDKAATAA 
      NPTIPQDLS SFDPFSSER KMDSNLLEM GDNFPDRKE 
      ADAKLDKFL QDLGPIAPP KHEEPPEIT NKDDKSATQ 
      HFMDFEREV SQPKKLDSH DLLEKYSDS EPYNDDDFK 
      PSKYQDFPK KAELPESIS SDFKTDTFK DLEEVEPQL 
      PKKDYPSAP EPEKVTPVP VREDKPAPV KEKTPEPVK 
      KLPEPAEVI CRKPELDEK KKSAVIDAE AIFCKMGLD 
      TWFNPERLN PKAVFVGTL PERRRFGRD SAYYSRSDL 
      VTSPP
    • >TC032949 RD
      MAEDIKNAA NALVDDVLN GVSNEMNKT RGGADDKSR 
      QRSLLCVLF TPNTWFNPE RLNPKAVFV GTLPERRRF 
      GRDSAYYSR SDLVTSPP
    • >TC032949 RE
      MDNKELSLE GVRDALRQS HSQPVGGLR REVDSLDDF 
      EHLGHDSSP LKDGGDLLS ARDQPVINK GDDKAATAA 
      NPTIPQDLS SFDPFSSER KMDSNLLEM GDNFPDRKE 
      ADAKLDKFL QDLGPIAPP KHEEPPEIT NKDDKSATQ 
      HFMDFEREV SQPKKLDSH DLLEKYSDS EPYNDDDFK 
      PSKYQDFPK KAELPESIS SDFKTDTFK DLEEVEPQL 
      PKKDYPSAP EPEKVTPVP VREDKPAPV KEKTPEPVK 
      KLPEPAEVI CRKPELDEK KKSAVIDAE AIFCKMGLG 
      KC
    CDS sequence
    • >TC032949 RA
      atggtacgc aaaaacagg taccagcaa tattccgaa 
      ccattgcct gaacgagga cccgtggaa agcctgatc 
      tattggcga gaccccaag aagtcaggg cctgtcttc 
      ggaggcgtc cttctggtg cttctagca ctcacatac 
      ttctcacta atcagtgtc gtggcatat ttgtcactg 
      ttgggcctt actggaacg atttccttc cggatttac 
      aaaaatatc gttcaagca gtacagaag acaggcgat 
      gggcacccc ttcaaagat cttctagag tttgatgtg 
      tcgcttccg caagaaaaa gtcaaagag gtgtcggaa 
      gtggccgtt gcccacatc aacgctgcg gtggtggag 
      ttgaggagg ttgttcctc gtcgaggat ttggtggat 
      tccatcaag ttcggggtt ttgttatgg acactgact 
      tacttaggg gcttggttc aatggaatg actttgatt 
      attattgct tgggtcgca ctcttttct cttcccaaa 
      gtttacgaa actaacaag acgcaaatt gatgccaat 
      ttggagatt gtgaggact aagctcgcc gaaatcacg 
      tcaaaggtg aaggcggcc attccaatt ggcaaaaaa 
      gcagaggag aagaaggat cagtaa
    • >TC032949 RB
      atggcggaa gatattaaa aatgccgcc aatgcgtta 
      gtcgacgac gtcctcaac ggtgtttcg aacgagatg 
      aacaagacc aggggtggt gccgacgat aagtcgaga 
      cagaggtca cttctttgc gttctattt actccaaac 
      acttggttc aaccctgaa agactgaat cctaaagtg 
      gaaagcctg atctattgg cgagacccc aagaagtca 
      gggcctgtc ttcggaggc gtccttctg gtgcttcta 
      gcactcaca tacttctca ctaatcagt gtcgtggca 
      tatttgtca ctgttgggc cttactgga acgatttcc 
      ttccggatt tacaaaaat atcgttcaa gcagtacag 
      aagacaggc gatgggcac cccttcaaa gatcttcta 
      gagtttgat gtgtcgctt ccgcaagaa aaagtcaaa 
      gaggtgtcg gaagtggcc gttgcccac atcaacgct 
      gcggtggtg gagttgagg aggttgttc ctcgtcgag 
      gatttggtg gattccatc aagttcggg gttttgtta 
      tggacactg acttactta ggggcttgg ttcaatgga 
      atgactttg attattatt gcttgggtc gcactcttt 
      tctcttccc aaagtttac gaaactaac aagacgcaa 
      attgatgcc aatttggag attgtgagg actaagctc 
      gccgaaatc acgtcaaag gtgaaggcg gccattcca 
      attggcaaa aaagcagag gagaagaag gatcagtaa
    • >TC032949 RC
      atggataac aaggaactg agtctggaa ggcgtccgc 
      gacgccctg cgccagagc cactcccag ccggttggg 
      ggccttcgg cgcgaagtg gactccttg gacgacttc 
      gagcacctg gggcacgac agctcgccg ctgaaagac 
      ggcggcgat ttgttgtcc gcacgtgac caacccgtg 
      atcaataaa ggggatgac aaagccgcc actgcagcc 
      aaccccaca attccccaa gacctgtca tcgttcgac 
      cctttctcc agcgagcga aagatggac tcgaacctc 
      ctcgaaatg ggcgacaac ttccccgac cgcaaagaa 
      gccgacgcc aaactcgac aagttcctc caggacttg 
      ggccccatt gcccccccg aagcacgaa gagccccca 
      gagatcacc aacaaggac gacaagagt gccacgcag 
      cacttcatg gacttcgag cgcgaggtg agtcaaccg 
      aagaaattg gactcgcac gacctcttg gagaagtac 
      tcagacagc gagccatac aatgacgac gacttcaag 
      ccttccaag tatcaggac ttccctaaa aaggccgaa 
      ctacccgaa tcgatatcg agcgatttt aagaccgac 
      accttcaag gatttggag gaagtcgaa ccgcaactt 
      cccaaaaaa gattatcct tccgctccg gaaccggaa 
      aaggtcacg cctgttcca gtacgagaa gacaaaccg 
      gctccggtt aaggagaag acgcccgaa ccggtgaag 
      aaactaccg gaaccggct gaagtcatc tgcaggaaa 
      ccggagctc gacgaaaag aagaagtca gcggttatt 
      gacgccgag gccatcttc tgcaaaatg ggcctagac 
      acttggttc aaccctgaa agactgaat cctaaagcc 
      gttttcgtg ggcacactg ccagaacgt cgtcgtttc 
      ggccgggat tccgcgtat tattcgcgt tccgacttg 
      gtgacctct cctccatag
    • >TC032949 RD
      atggcggaa gatattaaa aatgccgcc aatgcgtta 
      gtcgacgac gtcctcaac ggtgtttcg aacgagatg 
      aacaagacc aggggtggt gccgacgat aagtcgaga 
      cagaggtca cttctttgc gttctattt actccaaac 
      acttggttc aaccctgaa agactgaat cctaaagcc 
      gttttcgtg ggcacactg ccagaacgt cgtcgtttc 
      ggccgggat tccgcgtat tattcgcgt tccgacttg 
      gtgacctct cctccatag
    • >TC032949 RE
      atggataac aaggaactg agtctggaa ggcgtccgc 
      gacgccctg cgccagagc cactcccag ccggttggg 
      ggccttcgg cgcgaagtg gactccttg gacgacttc 
      gagcacctg gggcacgac agctcgccg ctgaaagac 
      ggcggcgat ttgttgtcc gcacgtgac caacccgtg 
      atcaataaa ggggatgac aaagccgcc actgcagcc 
      aaccccaca attccccaa gacctgtca tcgttcgac 
      cctttctcc agcgagcga aagatggac tcgaacctc 
      ctcgaaatg ggcgacaac ttccccgac cgcaaagaa 
      gccgacgcc aaactcgac aagttcctc caggacttg 
      ggccccatt gcccccccg aagcacgaa gagccccca 
      gagatcacc aacaaggac gacaagagt gccacgcag 
      cacttcatg gacttcgag cgcgaggtg agtcaaccg 
      aagaaattg gactcgcac gacctcttg gagaagtac 
      tcagacagc gagccatac aatgacgac gacttcaag 
      ccttccaag tatcaggac ttccctaaa aaggccgaa 
      ctacccgaa tcgatatcg agcgatttt aagaccgac 
      accttcaag gatttggag gaagtcgaa ccgcaactt 
      cccaaaaaa gattatcct tccgctccg gaaccggaa 
      aaggtcacg cctgttcca gtacgagaa gacaaaccg 
      gctccggtt aaggagaag acgcccgaa ccggtgaag 
      aaactaccg gaaccggct gaagtcatc tgcaggaaa 
      ccggagctc gacgaaaag aagaagtca gcggttatt 
      gacgccgag gccatcttc tgcaaaatg ggcctaggt 
      aagtgctaa
    mRNA sequence
    • >TC032949 RA
      tgaaggtga agtgtagcg cgaggtgtg ctgctccgg 
      ttacttgtg gttttttgt ttgaacaat tgtaacaat 
      ggtacgcaa aaacaggta ccagcaata ttccgaacc 
      attgcctga acgaggacc cgtggaaag cctgatcta 
      ttggcgaga ccccaagaa gtcagggcc tgtcttcgg 
      aggcgtcct tctggtgct tctagcact cacatactt 
      ctcactaat cagtgtcgt ggcatattt gtcactgtt 
      gggccttac tggaacgat ttccttccg gatttacaa 
      aaatatcgt tcaagcagt acagaagac aggcgatgg 
      gcacccctt caaagatct tctagagtt tgatgtgtc 
      gcttccgca agaaaaagt caaagaggt gtcggaagt 
      ggccgttgc ccacatcaa cgctgcggt ggtggagtt 
      gaggaggtt gttcctcgt cgaggattt ggtggattc 
      catcaagtt cggggtttt gttatggac actgactta 
      cttaggggc ttggttcaa tggaatgac tttgattat 
      tattgcttg ggtcgcact cttttctct tcccaaagt 
      ttacgaaac taacaagac gcaaattga tgccaattt 
      ggagattgt gaggactaa gctcgccga aatcacgtc 
      aaaggtgaa ggcggccat tccaattgg caaaaaagc 
      agaggagaa gaaggatca gtaattatt ttcaccgag 
      gagatagct gagatgtca cacttgtgt cataagtta 
      ttcaaattt agctattta tgaacaatc ttatgattt 
      attccttaa attccatcc tgaaataaa ttcaaacaa 
      atatatacg ctctgtata tacaccctg tttatttat 
      tgttgtatt gtaattcat aaattttat caatgcaac 
      tcgattcgg ttctgttgt ctttgtgta aagcgtttc 
      ttagttgaa cgtaaggaa ctactatta aaataaatt 
      ctatcgttt gttttcgtt ttatttctt ttgtgtgtt 
      attggatgc gatttatta tggctatta atattaggt 
      gataagatt ttcattaga ttaaaatgg gagattttg 
      aatatccta cacgtgtat aaattttac aatacacat 
      taatttttt acttacaaa catgatatt ttagtgatt 
      gtgttgtcc tttatgtga tgctgtagt aagagtgag 
      cacgcaatt tctatttgc caagtaaat ataaaaatt 
      atcgactgt tacttgaaa aatcagttg aaacatctt 
      tctagactg atggtgtaa atttccttt tttaacatt 
      taagaatta atgtagaac ttgttttaa tctgtttct 
      tcgacgaag aagaaacgt gagtaatgc cgtactaat 
      aatgttcag tgtcaatgt tatgtgtaa ctttacact 
      cattgaatg agatgttat cataaaaat gattgtatg 
      aagaccaag tcatctacg aatatctta tagttatat 
      ctaaaacag agttaatta taggcaaaa gaaaaatgt 
      ttaataatt gctatttat ttatgttag acatagttt 
      taatatcac ttttatttg ttgctcgtt tcgtcaagt 
      tgatgtaag ttgaggtga atatgttat tttagactg 
      ttgcatact tttataata gctttgtat ttgctttat 
      gagattatt aaattgttt atatgcgc
    • >TC032949 RB
      agtgggatt tagacactt tggactcga ggtcgcccc 
      atttgccac cgttgaagg ccaccacag gtgtcccaa 
      atttttttc cgtcgaata ttttgacta ggtcaacat 
      gtccttgtt cctgggggc acagtccca agtccccgg 
      cccccttca atccggtcg ataacaaca acgcttgcc 
      cagttaatc gctgtcagc gctgtcgag cacttgcgt 
      gtataggtc ggagtgaga gaaaaacta tacccactt 
      tggaaaata acaggcata gaacaacgc acatacagt 
      gtgtgcgtg ctgtattcg ttcaagtgt tttaaagaa 
      aaagtgttt tttgtttcg tggccgcga tggcggaag 
      atattaaaa atgccgcca atgcgttag tcgacgacg 
      tcctcaacg gtgtttcga acgagatga acaagacca 
      ggggtggtg ccgacgata agtcgagac agaggtcac 
      ttctttgcg ttctattta ctccaaaca cttggttca 
      accctgaaa gactgaatc ctaaagtgg aaagcctga 
      tctattggc gagacccca agaagtcag ggcctgtct 
      tcggaggcg tccttctgg tgcttctag cactcacat 
      acttctcac taatcagtg tcgtggcat atttgtcac 
      tgttgggcc ttactggaa cgatttcct tccggattt 
      acaaaaata tcgttcaag cagtacaga agacaggcg 
      atgggcacc ccttcaaag atcttctag agtttgatg 
      tgtcgcttc cgcaagaaa aagtcaaag aggtgtcgg 
      aagtggccg ttgcccaca tcaacgctg cggtggtgg 
      agttgagga ggttgttcc tcgtcgagg atttggtgg 
      attccatca agttcgggg ttttgttat ggacactga 
      cttacttag gggcttggt tcaatggaa tgactttga 
      ttattattg cttgggtcg cactctttt ctcttccca 
      aagtttacg aaactaaca agacgcaaa ttgatgcca 
      atttggaga ttgtgagga ctaagctcg ccgaaatca 
      cgtcaaagg tgaaggcgg ccattccaa ttggcaaaa 
      aagcagagg agaagaagg atcagtaat tattttcac 
      cgaggagat agctgagat gtcacactt gtgtcataa 
      gttattcaa atttagcta tttatgaac aatcttatg 
      atttattcc ttaaattcc atcctgaaa taaattcaa 
      acaaatata tacgctctg tatatacac cctgtttat 
      ttattgttg tattgtaat tcataaatt ttatcaatg 
      caactcgat tcggttctg ttgtctttg tgtaaagcg 
      tttcttagt tgaacgtaa ggaactact attaaaata 
      aattctatc gtttgtttt cgttttatt tcttttgtg 
      tgttattgg atgcgattt attatggct attaatatt 
      aggtgataa gattttcat tagattaaa atgggagat 
      tttgaatat cctacacgt gtataaatt ttacaatac 
      acattaatt ttttactta caaacatga tattttagt 
      gattgtgtt gtcctttat gtgatgctg tagtaagag 
      tgagcacgc aatttctat ttgccaagt aaatataaa 
      aattatcga ctgttactt gaaaaatca gttgaaaca 
      tctttctag actgatggt gtaaatttc cttttttaa 
      catttaaga attaatgta gaacttgtt ttaatctgt 
      ttcttcgac gaagaagaa acgtgagta atgccgtac 
      taataatgt tcagtgtca atgttatgt gtaacttta 
      cactcattg aatgagatg ttatcataa aaatgattg 
      tatgaagac caagtcatc tacgaatat cttatagtt 
      atatctaaa acagagtta attataggc aaaagaaaa 
      atgtttaat aattgctat ttatttatg ttagacata 
      gttttaata tcactttta tttgttgct cgtttcgtc 
      aagttgatg taagttgag gtgaatatg ttattttag 
      actgttgca tacttttat aatagcttt gtatttgct 
      ttatgagat tattaaatt gtttatatg cgc
    • >TC032949 RC
      cacagaatt ggaatgaat tccttattt ctgaaaggt 
      atttttgaa aattttgga gaaatgtcc tcaaaagaa 
      atgaatttt atttaaatg agttgggta atttgcgat 
      tggtcacat gaaaaagca tcagccaat caaatggct 
      tgaaaacca ccccctgtt tcgcaacat tgcacgttg 
      ttgtgttac gagtccgtt gtttccgat gtgtttaca 
      ttggggaat accaattaa aaattgcca aatgtcgcc 
      ggcttattt ataattggt ccaaatggg cgtgttatt 
      tccataaat cagtccatc cccgcttct cctttctct 
      tccacgcca atggcatcc caccctggt cccccactc 
      ctcagaaaa catcctgca acaaaagca tcgcacacc 
      aacacaacc aagttttct gacgtttga cagttgtgc 
      cggttgtgt ttgtgaaat tcgtagtgt ttgtctctt 
      atttgtgaa agaaaaaac tgataaata aaaaagtgt 
      cgatcatgg ataacaagg aactgagtc tggaaggcg 
      tccgcgacg ccctgcgcc agagccact cccagccgg 
      ttgggggcc ttcggcgcg aagtggact ccttggacg 
      acttcgagc acctggggc acgacagct cgccgctga 
      aagacggcg gcgatttgt tgtccgcac gtgaccaac 
      ccgtgatca ataaagggg atgacaaag ccgccactg 
      cagccaacc ccacaattc cccaagacc tgtcatcgt 
      tcgaccctt tctccagcg agcgaaaga tggactcga 
      acctcctcg aaatgggcg acaacttcc ccgaccgca 
      aagaagccg acgccaaac tcgacaagt tcctccagg 
      acttgggcc ccattgccc ccccgaagc acgaagagc 
      ccccagaga tcaccaaca aggacgaca agagtgcca 
      cgcagcact tcatggact tcgagcgcg aggtgagtc 
      aaccgaaga aattggact cgcacgacc tcttggaga 
      agtactcag acagcgagc catacaatg acgacgact 
      tcaagcctt ccaagtatc aggacttcc ctaaaaagg 
      ccgaactac ccgaatcga tatcgagcg attttaaga 
      ccgacacct tcaaggatt tggaggaag tcgaaccgc 
      aacttccca aaaaagatt atccttccg ctccggaac 
      cggaaaagg tcacgcctg ttccagtac gagaagaca 
      aaccggctc cggttaagg agaagacgc ccgaaccgg 
      tgaagaaac taccggaac cggctgaag tcatctgca 
      ggaaaccgg agctcgacg aaaagaaga agtcagcgg 
      ttattgacg ccgaggcca tcttctgca aaatgggcc 
      tagacactt ggttcaacc ctgaaagac tgaatccta 
      aagccgttt tcgtgggca cactgccag aacgtcgtc 
      gtttcggcc gggattccg cgtattatt cgcgttccg 
      acttggtga cctctcctc cataggctg catgctcct 
      tctttcgcc atgagcgtc ttcgatgtg ttgcgtcgt 
      gatgagcgt aagttgcgc tttccataa acgattttt 
      ccccctgtg tgtggctgc ggcggctgg atcggtttc 
      tagtgaccg gaccgattc agttttttt ttagttttt 
      gcgaaaaat ctacggcca gtcgagcga cgcaacagt 
      acgtgagaa tttt
    • >TC032949 RD
      agtgggatt tagacactt tggactcga ggtcgcccc 
      atttgccac cgttgaagg ccaccacag gtgtcccaa 
      atttttttc cgtcgaata ttttgacta ggtcaacat 
      gtccttgtt cctgggggc acagtccca agtccccgg 
      cccccttca atccggtcg ataacaaca acgcttgcc 
      cagttaatc gctgtcagc gctgtcgag cacttgcgt 
      gtataggtc ggagtgaga gaaaaacta tacccactt 
      tggaaaata acaggcata gaacaacgc acatacagt 
      gtgtgcgtg ctgtattcg ttcaagtgt tttaaagaa 
      aaagtgttt tttgtttcg tggccgcga tggcggaag 
      atattaaaa atgccgcca atgcgttag tcgacgacg 
      tcctcaacg gtgtttcga acgagatga acaagacca 
      ggggtggtg ccgacgata agtcgagac agaggtcac 
      ttctttgcg ttctattta ctccaaaca cttggttca 
      accctgaaa gactgaatc ctaaagccg ttttcgtgg 
      gcacactgc cagaacgtc gtcgtttcg gccgggatt 
      ccgcgtatt attcgcgtt ccgacttgg tgacctctc 
      ctccatagg ctgcatgct ccttctttc gccatgagc 
      gtcttcgat gtgttgcgt cgtgatgag cgtaagttg 
      cgctttcca taaacgatt tttccccct gtgtgtggc 
      tgcggcggc tggatcggt ttctagtga ccggaccga 
      ttcagtttt tttttagtt tttgcgaaa aatctacgg 
      ccagtcgag cgacgcaac agtacgtga gaatttt
    • >TC032949 RE
      cacagaatt ggaatgaat tccttattt ctgaaaggt 
      atttttgaa aattttgga gaaatgtcc tcaaaagaa 
      atgaatttt atttaaatg agttgggta atttgcgat 
      tggtcacat gaaaaagca tcagccaat caaatggct 
      tgaaaacca ccccctgtt tcgcaacat tgcacgttg 
      ttgtgttac gagtccgtt gtttccgat gtgtttaca 
      ttggggaat accaattaa aaattgcca aatgtcgcc 
      ggcttattt ataattggt ccaaatggg cgtgttatt 
      tccataaat cagtccatc cccgcttct cctttctct 
      tccacgcca atggcatcc caccctggt cccccactc 
      ctcagaaaa catcctgca acaaaagca tcgcacacc 
      aacacaacc aagttttct gacgtttga cagttgtgc 
      cggttgtgt ttgtgaaat tcgtagtgt ttgtctctt 
      atttgtgaa agaaaaaac tgataaata aaaaagtgt 
      cgatcatgg ataacaagg aactgagtc tggaaggcg 
      tccgcgacg ccctgcgcc agagccact cccagccgg 
      ttgggggcc ttcggcgcg aagtggact ccttggacg 
      acttcgagc acctggggc acgacagct cgccgctga 
      aagacggcg gcgatttgt tgtccgcac gtgaccaac 
      ccgtgatca ataaagggg atgacaaag ccgccactg 
      cagccaacc ccacaattc cccaagacc tgtcatcgt 
      tcgaccctt tctccagcg agcgaaaga tggactcga 
      acctcctcg aaatgggcg acaacttcc ccgaccgca 
      aagaagccg acgccaaac tcgacaagt tcctccagg 
      acttgggcc ccattgccc ccccgaagc acgaagagc 
      ccccagaga tcaccaaca aggacgaca agagtgcca 
      cgcagcact tcatggact tcgagcgcg aggtgagtc 
      aaccgaaga aattggact cgcacgacc tcttggaga 
      agtactcag acagcgagc catacaatg acgacgact 
      tcaagcctt ccaagtatc aggacttcc ctaaaaagg 
      ccgaactac ccgaatcga tatcgagcg attttaaga 
      ccgacacct tcaaggatt tggaggaag tcgaaccgc 
      aacttccca aaaaagatt atccttccg ctccggaac 
      cggaaaagg tcacgcctg ttccagtac gagaagaca 
      aaccggctc cggttaagg agaagacgc ccgaaccgg 
      tgaagaaac taccggaac cggctgaag tcatctgca 
      ggaaaccgg agctcgacg aaaagaaga agtcagcgg 
      ttattgacg ccgaggcca tcttctgca aaatgggcc 
      taggtaagt gctaaaaag gggaacccg gggaacaga 
      tgttgatga ttcatcgta ttatgacat ggaattccg 
      gtattatga cgcatgtct gatgcccaa atgaaattg 
      ttttttttg ttactattg tattttttc gattttatt 
      gtcattcaa tacgttctt ttaattaca tgatggttg 
      tcgctattt tagttaaaa ggtggcaag tttttccgt 
      gagctgacg tcacgtgca gccatgcaa atatttatc 
      gaaccgtgt tgtatttta cttggaaca agtgtatac 
      agttgggtt attatggca attttatat ctcagctgt 
      gccatcctc ctaaaactt cgatcgttt ggatgaaaa 
      aaaaaaaca aaccctcat cttttatta ttgcagtag 
      ataagaatt attagtagt gacggtcgc aaggtgatg 
      ataataaac tagtcatga attc
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