Status
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Gene information
General information
TC008382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9573053..9576815
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008382
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (22 dpi)
    Days post injection


    Pupal morphology
    elytra not closed - Number of animals showing the phenotype: 8

    oblique dorsal thoracic muscles slightly medianshortened - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008382 RA
      MENPEEILH SLEEFSKTK PKDIPRELE EYLCFVAKT 
      GDPVYQWSA IKSLFREKM INVITEFYE TCPSVEIPP 
      CPNVDIFNY DLMKNFILE KLDTFAAAP FTLQRICEL 
      LTTPRKEYN RIDKYMRAL EKNILVVST TEPGGRRNT 
      ENGEGIMNG LESEHLPET SNSSNDINV EEMDDAPAW 
      PRLLQNENV PYQNENSNS NDIQTQPIV QETVVTASK 
      LPDVACSSS QDTVITCRS SEEPYVSIE PVPSNTCTR 
      SDPDETDVS VTITAIPAG VQKRRNSVE PVVSSETEE 
      ITESCDQSD KTSEPSKES EAKQEETPS SQQIEDADS 
      SSSAEDDKE SVESKEEKT VDDAVPQSE LSSSECDKN 
      EDEVGTSQE KLDENPDES EEITVDTTK CSNASPTPQ 
      EETKEDLEI TSSDKIDEV HSSQSEAEA KIEETEKCE 
      ESQAAPEEG VKEASEEAA TAMDVDEFS EKVEEVVRA 
      EATSDSPL
    CDS sequence
    • >TC008382 RA
      atggaaaat cccgaagaa atactccac agtttagag 
      gaattctcg aaaaccaag cccaaggac ataccgcgc 
      gaactggag gagtacctt tgttttgtg gcgaaaaca 
      ggggaccct gtgtaccag tggtcggca ataaagagc 
      ctctttcgc gagaagatg attaacgtt ataacggag 
      ttctacgag acgtgccct tcggtcgaa ataccccct 
      tgtcccaac gtggacatt ttcaattac gaccttatg 
      aagaatttt attttagag aagttggac acgtttgcg 
      gcggccccc ttcacgtta cagaggatt tgcgagctg 
      ttgacgact ccgaggaag gagtataat cggatagac 
      aagtacatg agggccctg gaaaagaat attctggtt 
      gtgagcacc actgaacct gggggcagg aggaacacg 
      gagaacggg gagggcatt atgaacggg ttggagtcg 
      gaacatttg ccggaaact agcaatagc agcaatgac 
      atcaacgtg gaagaaatg gacgatgcg ccagcttgg 
      cctagattg ctacagaac gaaaatgtt ccatatcaa 
      aatgagaat agcaacagt aatgacatt caaacccag 
      ccaattgta caagaaacg gtggtcaca gcgtctaaa 
      ctaccagat gtggcctgc agctcgtct caagacact 
      gtcataacg tgtcgaagc tcagaggag ccctacgtt 
      tcgattgaa cctgtgcca tcaaatact tgcaccaga 
      tcagaccca gatgagacg gatgtctcg gttaccatc 
      actgccata cctgcaggg gtccaaaag agacgaaac 
      agtgtcgaa ccggtcgtt tcgtcagaa accgaagaa 
      atcaccgag tcttgtgac caatctgac aaaaccagc 
      gaaccttcc aaagaatct gaggctaaa caagaggaa 
      acgccttcg tcgcagcaa atcgaggac gcagactcg 
      tcctcaagc gctgaagac gacaaagaa tcggttgaa 
      agtaaggag gagaaaact gtggacgat gcggtccct 
      cagagcgag ttatcgtcg tctgagtgc gataaaaac 
      gaggatgaa gttggcaca tcgcaagag aaacttgac 
      gagaatccg gacgaaagc gaagaaata actgtcgat 
      accaccaag tgttcaaat gcctcccca acaccacag 
      gaagaaact aaagaagac ctcgaaatt acgtcttct 
      gataaaatc gatgaggtg cactcgtca caaagcgaa 
      gctgaagcg aaaattgaa gagactgag aaatgtgaa 
      gaatctcaa gctgctccc gaagaaggg gtgaaagag 
      gcaagtgaa gaggcggcg actgcgatg gatgtggat 
      gagtttagc gaaaaagtg gaagaggtt gtcagggct 
      gaagctacg tcagactca cctttataa
    mRNA sequence
    • >TC008382 RA
      atattgaac cggcgctaa cttcgttat tgtcattgg 
      tgcgcttgt tgacgcaag tagatggtg atcggtggt 
      gctaaaatt tcacgaaaa aaacaataa caataatgg 
      aaaatcccg aagaaatac tccacagtt tagaggaat 
      tctcgaaaa ccaagccca aggacatac cgcgcgaac 
      tggaggagt acctttgtt ttgtggcga aaacagggg 
      accctgtgt accagtggt cggcaataa agagcctct 
      ttcgcgaga agatgatta acgttataa cggagttct 
      acgagacgt gcccttcgg tcgaaatac ccccttgtc 
      ccaacgtgg acattttca attacgacc ttatgaaga 
      attttattt tagagaagt tggacacgt ttgcggcgg 
      cccccttca cgttacaga ggatttgcg agctgttga 
      cgactccga ggaaggagt ataatcgga tagacaagt 
      acatgaggg ccctggaaa agaatattc tggttgtga 
      gcaccactg aacctgggg gcaggagga acacggaga 
      acggggagg gcattatga acgggttgg agtcggaac 
      atttgccgg aaactagca atagcagca atgacatca 
      acgtggaag aaatggacg atgcgccag cttggccta 
      gattgctac agaacgaaa atgttccat atcaaaatg 
      agaatagca acagtaatg acattcaaa cccagccaa 
      ttgtacaag aaacggtgg tcacagcgt ctaaactac 
      cagatgtgg cctgcagct cgtctcaag acactgtca 
      taacgtgtc gaagctcag aggagccct acgtttcga 
      ttgaacctg tgccatcaa atacttgca ccagatcag 
      acccagatg agacggatg tctcggtta ccatcactg 
      ccatacctg caggggtcc aaaagagac gaaacagtg 
      tcgaaccgg tcgtttcgt cagaaaccg aagaaatca 
      ccgagtctt gtgaccaat ctgacaaaa ccagcgaac 
      cttccaaag aatctgagg ctaaacaag aggaaacgc 
      cttcgtcgc agcaaatcg aggacgcag actcgtcct 
      caagcgctg aagacgaca aagaatcgg ttgaaagta 
      aggaggaga aaactgtgg acgatgcgg tccctcaga 
      gcgagttat cgtcgtctg agtgcgata aaaacgagg 
      atgaagttg gcacatcgc aagagaaac ttgacgaga 
      atccggacg aaagcgaag aaataactg tcgatacca 
      ccaagtgtt caaatgcct ccccaacac cacaggaag 
      aaactaaag aagacctcg aaattacgt cttctgata 
      aaatcgatg aggtgcact cgtcacaaa gcgaagctg 
      aagcgaaaa ttgaagaga ctgagaaat gtgaagaat 
      ctcaagctg ctcccgaag aaggggtga aagaggcaa 
      gtgaagagg cggcgactg cgatggatg tggatgagt 
      ttagcgaaa aagtggaag aggttgtca gggctgaag 
      ctacgtcag actcacctt tataattta gataatttt 
      tttccttaa ttaacgatc aaggctaac aaacgcgcc 
      ttcttaatt gttaagtat ttatacaat gtgtaatta 
      tgcaaacga agttttttc attttacta gaatctgta 
      gggttcgtc tgattcgtc aacaaaatt cgttttttt 
      taaatttgt atgtagagc gtaggtaaa taaatttgt 
      acgtaataa agaactgaa taaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY