Status
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Gene information
General information
TC031113
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008367,TC008368,TC008366
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9189608..9208820
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031113
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04475

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head split - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031113 RA
      MEAKLPVIL FLLAIKQQT SEIEATPIA EVNVNNFED 
      AWDYKVEPN KMPVLSESP KNHNLPPPA KVEIPEKKF 
      VKPAPLPER VAKKIGDAV RARVSCPIC RLGVGLLQK 
      EVKRNTSFE DIRTKFVGL CVAFKVEGE DVCAGIFDV 
      FGPDVLPAL RVVQIGPDE ICALVLGEV CGNVKIPLH 
      EWQVEFPDV PKPNITKRD VPKAGLPTF KVLQISDTH 
      FDPDYVVGS VANCEEPLC CRSTSTPPL VGEVAPAGK 
      WGSYQKCDA PKVLIDNML KSIAEEHPD IDYVIWTGD 
      LPPHDIWNQ TKQSNLDII SETVEQMFE HFPNTPIFP 
      ALGNHESIP AGSFAPPWV QDPDRSIAW LYTKVADHW 
      RKWLPASAG NTVLHGGFY SVLIRPGFR IISLNMNYC 
      HTLSWWLIV NSTDPAKEL KWLVYELQE AENNGEKVH 
      LIGHIPPGS SDCMKVWSR NFNKIIERY ENTIQAQFY 
      GHTHADEFE VLYDVEESS RPINVAYLG PSVTTFENH 
      NPAYRIYYV DGDHENTTR EILDHETWT FDLEEANKG 
      NSDPKWYRL YSAKNAYKM DSLRPEAWA KLINDLVES 
      PDLFELFYK HYYRDSPVR PTCDRKCKL QILCDLKSG 
      RSHVKHQLC HDLELTFVT PQDISALVD VKTLSELPL 
      EKLLQVKST FQEPETEAK ADKKVDGPS ALALQSKRK 
      PNDRGGYHY HYEEENSKK SGKKSVQSI FQISVTTLA 
      FLAFGGYLL CLLVQAIKG KTMMNMMDM TTMPPTGAA 
      LFRPRPTRR RRRRPTRRP VKLQRPTRQ TYGRKPREK 
      REVWPEADP ESMYYVLVH LSEAYVRYH TIDYRKFNY 
      TASSYANMG FARSKPGGE PDIPKMDMS RTKAEKDPL 
      KFGIDRLLT SDPCKDNKS VVAKPLATV PVPCSDCVT 
      SLFRCCRLS PSAQDNIGE FFGQHHGLS VTPTSSIYT 
      VQPIRPFAT RPAVRIPVP PPPPATSPS LNINQNNNK 
      RKRSWSRAV FSNLQRKGL ERRFQLQKY ITKPDRRQL 
      AATLGLTDA QVKVWFQNR RMKWRHSKE AGKGGENSA 
      DSTQDQDVH IDVDTITDN D
    CDS sequence
    • >TC031113 RA
      atggaagcc aaactaccg gtcatcctc tttctccta 
      gcaattaaa cagcaaaca tccgaaata gaagccact 
      cctatcgcc gaagtgaac gtaaacaat ttcgaagat 
      gcctgggat tacaaagtg gagccaaac aaaatgcca 
      gttttgagc gaatcgcca aaaaatcac aatttgcct 
      ccgccagcc aaggtcgaa ataccggag aagaagttc 
      gtcaagccg gctccacta ccagaacga gtcgccaaa 
      aaaatcgga gatgctgtt agagcacga gtttcttgc 
      ccgatatgt agattagga gtgggcctc ttacaaaag 
      gaagtcaaa agaaacaca agctttgag gacatccga 
      accaaattt gtgggactt tgcgtcgcc ttcaaagtc 
      gaaggtgag gacgtgtgt gccggaatc ttcgacgtg 
      ttcggcccc gatgtctta ccggcgctc cgagtggtg 
      caaattgga cccgacgag atctgcgct ctagtcctt 
      ggcgaagtc tgcggtaac gttaaaata cccctccac 
      gagtggcaa gtggaattt cccgacgtc cccaagccc 
      aacatcacg aaacgcgac gtaccaaaa gcaggtttg 
      ccgactttc aaggttttg caaatttcc gatacacac 
      tttgacccc gattatgtc gtggggagt gtggccaac 
      tgcgaggag cccctctgt tgccgcagc actagcact 
      ccgcccttg gtgggcgaa gtggctcca gccgggaaa 
      tggggcagt taccaaaag tgtgacgca cccaaagtt 
      ttgattgat aacatgttg aaaagcata gcggaagag 
      catcctgac atagattat gttatttgg accggtgat 
      ttgccccct catgacatt tggaaccag accaagcaa 
      agcaacttg gacataatc agcgaaacg gtggagcaa 
      atgtttgaa cacttcccc aacactcct atttttcca 
      gcgttgggg aaccatgaa tctatcccg gctgggagt 
      tttgcccca ccatgggtc caggacccc gacaggtcc 
      attgcctgg ctgtacacc aaggtggcc gaccattgg 
      aggaaatgg ttgccggcg agcgccggc aatacggta 
      ctccatgga gggttctac tctgtttta atcagacca 
      ggattccgc attatttcg ctcaatatg aactactgt 
      cacacactt agttggtgg ctcatagtc aacagtacc 
      gacccagcc aaggaatta aaatggcta gtgtatgag 
      ttgcaagag gctgaaaac aatggcgaa aaagtccac 
      ctaattggc cacatcccg cctggaagt tccgactgt 
      atgaaggta tggtcccgc aactttaat aaaataatc 
      gaacggtat gaaaacacg atccaagcc caattctac 
      ggtcacaca catgccgat gaattcgaa gttttgtac 
      gacgttgaa gaaagcagc cgaccaatc aatgtggct 
      tatctggga ccttcagtt accactttc gaaaatcac 
      aatccagct tatcgcata tattatgta gatggtgac 
      catgaaaac accaccaga gaaattctc gaccatgag 
      acttggact tttgactta gaggaagcc aataagggt 
      aatagcgac ccgaaatgg taccgatta tactcggcc 
      aaaaatgcc tacaaaatg gactctctc cgcccggaa 
      gcttgggca aaactcatt aatgaccta gtcgaaagc 
      ccggattta ttcgaacta ttttacaaa cattattat 
      agggacagc ccagtgaga ccaacctgt gaccgtaaa 
      tgtaagctc caaatattg tgtgacctc aaaagtgga 
      agatcccac gtcaaacac cagttatgt cacgactta 
      gaattaacc ttcgtgacc cctcaagac atttccgcg 
      ttggtcgat gtgaaaact ctgagtgaa cttccctta 
      gaaaagctc ctccaagtg aaaagcacg ttccaagaa 
      ccggaaact gaggccaag gctgataag aaagtggat 
      gggccgtca gcgctggcc ctccagtca aagcgcaaa 
      ccgaacgac cgagggggc tatcactac cactatgag 
      gaggaaaat tcgaagaaa agtggcaaa aagagtgtg 
      caaagtatt ttccaaatt tccgtgacg actctagct 
      tttctggcg tttggaggg tatttgttg tgtctgttg 
      gtgcaagcc atcaagggc aagactatg atgaatatg 
      atggacatg acgacgatg ccgccaact ggggccgct 
      ctcttccgg cccaggccc acaaggagg cggcggcgg 
      cggcccacg cgaaggccc gtcaagttg caaaggccc 
      acgagacaa acctacggg aggaaaccg agggaaaaa 
      cgcgaagtt tggcctgaa gccgaccct gagagcatg 
      tactacgtc ctcgtgcac ctctctgaa gcctacgtc 
      cgttatcac accatcgat tatagaaaa ttcaattac 
      acggcgagc tcttatgcg aacatgggt ttcgctcgt 
      tcaaaacca ggaggcgaa ccagacatc ccgaaaatg 
      gatatgtcc aggaccaaa gcggaaaag gacccgctc 
      aagttcggc atcgacaga ctcctgacc agtgatcct 
      tgcaaggat aataaaagt gttgtggct aaaccttta 
      gcaactgtt ccagtgcct tgttccgac tgtgttacg 
      tcgctgttt aggtgttgt cggttgagt ccttcggca 
      caggacaat atcggggag tttttcgga cagcatcat 
      gggttgagt gtcacaccc acgtccagt atttataca 
      gtgcagccc atcaggccc tttgccaca aggccagct 
      gtgcgcatc ccggtgcca ccgccgccc ccagcgacg 
      tcgccaagc ttaaacatc aaccagaac aacaataag 
      aggaagagg tcctggtca agggccgtt tttagcaat 
      ttgcaaagg aaaggattg gaaaggcgg tttcagttg 
      cagaagtac atcacgaag ccggatagg agacaactt 
      gccgctact ttgggtctg acagatgca caagtgaaa 
      gtctggttt cagaacagg aggatgaag tggaggcat 
      tcgaaagaa gcgggaaaa ggaggcgaa aacagtgca 
      gactccacg caggatcaa gatgtgcat atagatgtc 
      gatacgatc accgataat gactga
    mRNA sequence
    • >TC031113 RA
      gacgttata acaaatcga aactagaca ataattagg 
      aatcaacga tgtgtagtt tgtatttag tggacagcc 
      ttgaatgtt gaactacag aataaaata ttaaggttt 
      aaataattc ctcttgatt cgcaagtga aaactcgtg 
      gagacaaga tggaagcca aactaccgg tcatcctct 
      ttctcctag caattaaac agcaaacat ccgaaatag 
      aagccactc ctatcgccg aagtgaacg taaacaatt 
      tcgaagatg cctgggatt acaaagtgg agccaaaca 
      aaatgccag ttttgagcg aatcgccaa aaaatcaca 
      atttgcctc cgccagcca aggtcgaaa taccggaga 
      agaagttcg tcaagccgg ctccactac cagaacgag 
      tcgccaaaa aaatcggag atgctgtta gagcacgag 
      tttcttgcc cgatatgta gattaggag tgggcctct 
      tacaaaagg aagtcaaaa gaaacacaa gctttgagg 
      acatccgaa ccaaatttg tgggacttt gcgtcgcct 
      tcaaagtcg aaggtgagg acgtgtgtg ccggaatct 
      tcgacgtgt tcggccccg atgtcttac cggcgctcc 
      gagtggtgc aaattggac ccgacgaga tctgcgctc 
      tagtccttg gcgaagtct gcggtaacg ttaaaatac 
      ccctccacg agtggcaag tggaatttc ccgacgtcc 
      ccaagccca acatcacga aacgcgacg taccaaaag 
      caggtttgc cgactttca aggttttgc aaatttccg 
      atacacact ttgaccccg attatgtcg tggggagtg 
      tggccaact gcgaggagc ccctctgtt gccgcagca 
      ctagcactc cgcccttgg tgggcgaag tggctccag 
      ccgggaaat ggggcagtt accaaaagt gtgacgcac 
      ccaaagttt tgattgata acatgttga aaagcatag 
      cggaagagc atcctgaca tagattatg ttatttgga 
      ccggtgatt tgccccctc atgacattt ggaaccaga 
      ccaagcaaa gcaacttgg acataatca gcgaaacgg 
      tggagcaaa tgtttgaac acttcccca acactccta 
      tttttccag cgttgggga accatgaat ctatcccgg 
      ctgggagtt ttgccccac catgggtcc aggaccccg 
      acaggtcca ttgcctggc tgtacacca aggtggccg 
      accattgga ggaaatggt tgccggcga gcgccggca 
      atacggtac tccatggag ggttctact ctgttttaa 
      tcagaccag gattccgca ttatttcgc tcaatatga 
      actactgtc acacactta gttggtggc tcatagtca 
      acagtaccg acccagcca aggaattaa aatggctag 
      tgtatgagt tgcaagagg ctgaaaaca atggcgaaa 
      aagtccacc taattggcc acatcccgc ctggaagtt 
      ccgactgta tgaaggtat ggtcccgca actttaata 
      aaataatcg aacggtatg aaaacacga tccaagccc 
      aattctacg gtcacacac atgccgatg aattcgaag 
      ttttgtacg acgttgaag aaagcagcc gaccaatca 
      atgtggctt atctgggac cttcagtta ccactttcg 
      aaaatcaca atccagctt atcgcatat attatgtag 
      atggtgacc atgaaaaca ccaccagag aaattctcg 
      accatgaga cttggactt ttgacttag aggaagcca 
      ataagggta atagcgacc cgaaatggt accgattat 
      actcggcca aaaatgcct acaaaatgg actctctcc 
      gcccggaag cttgggcaa aactcatta atgacctag 
      tcgaaagcc cggatttat tcgaactat tttacaaac 
      attattata gggacagcc cagtgagac caacctgtg 
      accgtaaat gtaagctcc aaatattgt gtgacctca 
      aaagtggaa gatcccacg tcaaacacc agttatgtc 
      acgacttag aattaacct tcgtgaccc ctcaagaca 
      tttccgcgt tggtcgatg tgaaaactc tgagtgaac 
      ttcccttag aaaagctcc tccaagtga aaagcacgt 
      tccaagaac cggaaactg aggccaagg ctgataaga 
      aagtggatg ggccgtcag cgctggccc tccagtcaa 
      agcgcaaac cgaacgacc gagggggct atcactacc 
      actatgagg aggaaaatt cgaagaaaa gtggcaaaa 
      agagtgtgc aaagtattt tccaaattt ccgtgacga 
      ctctagctt ttctggcgt ttggagggt atttgttgt 
      gtctgttgg tgcaagcca tcaagggca agactatga 
      tgaatatga tggacatga cgacgatgc cgccaactg 
      gggccgctc tcttccggc ccaggccca caaggaggc 
      ggcggcggc ggcccacgc gaaggcccg tcaagttgc 
      aaaggccca cgagacaaa cctacggga ggaaaccga 
      gggaaaaac gcgaagttt ggcctgaag ccgaccctg 
      agagcatgt actacgtcc tcgtgcacc tctctgaag 
      cctacgtcc gttatcaca ccatcgatt atagaaaat 
      tcaattaca cggcgagct cttatgcga acatgggtt 
      tcgctcgtt caaaaccag gaggcgaac cagacatcc 
      cgaaaatgg atatgtcca ggaccaaag cggaaaagg 
      acccgctca agttcggca tcgacagac tcctgacca 
      gtgatcctt gcaaggata ataaaagtg ttgtggcta 
      aacctttag caactgttc cagtgcctt gttccgact 
      gtgttacgt cgctgttta ggtgttgtc ggttgagtc 
      cttcggcac aggacaata tcggggagt ttttcggac 
      agcatcatg ggttgagtg tcacaccca cgtccagta 
      tttatacag tgcagccca tcaggccct ttgccacaa 
      ggccagctg tgcgcatcc cggtgccac cgccgcccc 
      cagcgacgt cgccaagct taaacatca accagaaca 
      acaataaga ggaagaggt cctggtcaa gggccgttt 
      ttagcaatt tgcaaagga aaggattgg aaaggcggt 
      ttcagttgc agaagtaca tcacgaagc cggatagga 
      gacaacttg ccgctactt tgggtctga cagatgcac 
      aagtgaaag tctggtttc agaacagga ggatgaagt 
      ggaggcatt cgaaagaag cgggaaaag gaggcgaaa 
      acagtgcag actccacgc aggatcaag atgtgcata 
      tagatgtcg atacgatca ccgataatg actgattct 
      tctaacttt gtacataaa tgttttatt attaatact 
      ttgaagttt tatttatac ccggcaaag tatacttaa 
      gttgcaatt tttttcagc aaagtggca tttattcag 
      aagaaaagc aaaattaaa at
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