Status
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Gene information
General information
TC031113
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008367,TC008368,TC008366 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9189608..9208820
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031113
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04475

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head split - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031113 RA
      MEAKLPVIL FLLAIKQQT SEIEATPIA EVNVNNFED 
AWDYKVEPN KMPVLSESP KNHNLPPPA KVEIPEKKF 
VKPAPLPER VAKKIGDAV RARVSCPIC RLGVGLLQK 
EVKRNTSFE DIRTKFVGL CVAFKVEGE DVCAGIFDV 
FGPDVLPAL RVVQIGPDE ICALVLGEV CGNVKIPLH 
EWQVEFPDV PKPNITKRD VPKAGLPTF KVLQISDTH 
FDPDYVVGS VANCEEPLC CRSTSTPPL VGEVAPAGK 
WGSYQKCDA PKVLIDNML KSIAEEHPD IDYVIWTGD 
LPPHDIWNQ TKQSNLDII SETVEQMFE HFPNTPIFP 
ALGNHESIP AGSFAPPWV QDPDRSIAW LYTKVADHW 
RKWLPASAG NTVLHGGFY SVLIRPGFR IISLNMNYC 
HTLSWWLIV NSTDPAKEL KWLVYELQE AENNGEKVH 
LIGHIPPGS SDCMKVWSR NFNKIIERY ENTIQAQFY 
GHTHADEFE VLYDVEESS RPINVAYLG PSVTTFENH 
NPAYRIYYV DGDHENTTR EILDHETWT FDLEEANKG 
NSDPKWYRL YSAKNAYKM DSLRPEAWA KLINDLVES 
PDLFELFYK HYYRDSPVR PTCDRKCKL QILCDLKSG 
RSHVKHQLC HDLELTFVT PQDISALVD VKTLSELPL 
EKLLQVKST FQEPETEAK ADKKVDGPS ALALQSKRK 
PNDRGGYHY HYEEENSKK SGKKSVQSI FQISVTTLA 
FLAFGGYLL CLLVQAIKG KTMMNMMDM TTMPPTGAA 
LFRPRPTRR RRRRPTRRP VKLQRPTRQ TYGRKPREK 
REVWPEADP ESMYYVLVH LSEAYVRYH TIDYRKFNY 
TASSYANMG FARSKPGGE PDIPKMDMS RTKAEKDPL 
KFGIDRLLT SDPCKDNKS VVAKPLATV PVPCSDCVT 
SLFRCCRLS PSAQDNIGE FFGQHHGLS VTPTSSIYT 
VQPIRPFAT RPAVRIPVP PPPPATSPS LNINQNNNK 
RKRSWSRAV FSNLQRKGL ERRFQLQKY ITKPDRRQL 
AATLGLTDA QVKVWFQNR RMKWRHSKE AGKGGENSA 
DSTQDQDVH IDVDTITDN D
    CDS sequence
    • >TC031113 RA
      atggaagcc aaactaccg gtcatcctc tttctccta 
gcaattaaa cagcaaaca tccgaaata gaagccact 
cctatcgcc gaagtgaac gtaaacaat ttcgaagat 
gcctgggat tacaaagtg gagccaaac aaaatgcca 
gttttgagc gaatcgcca aaaaatcac aatttgcct 
ccgccagcc aaggtcgaa ataccggag aagaagttc 
gtcaagccg gctccacta ccagaacga gtcgccaaa 
aaaatcgga gatgctgtt agagcacga gtttcttgc 
ccgatatgt agattagga gtgggcctc ttacaaaag 
gaagtcaaa agaaacaca agctttgag gacatccga 
accaaattt gtgggactt tgcgtcgcc ttcaaagtc 
gaaggtgag gacgtgtgt gccggaatc ttcgacgtg 
ttcggcccc gatgtctta ccggcgctc cgagtggtg 
caaattgga cccgacgag atctgcgct ctagtcctt 
ggcgaagtc tgcggtaac gttaaaata cccctccac 
gagtggcaa gtggaattt cccgacgtc cccaagccc 
aacatcacg aaacgcgac gtaccaaaa gcaggtttg 
ccgactttc aaggttttg caaatttcc gatacacac 
tttgacccc gattatgtc gtggggagt gtggccaac 
tgcgaggag cccctctgt tgccgcagc actagcact 
ccgcccttg gtgggcgaa gtggctcca gccgggaaa 
tggggcagt taccaaaag tgtgacgca cccaaagtt 
ttgattgat aacatgttg aaaagcata gcggaagag 
catcctgac atagattat gttatttgg accggtgat 
ttgccccct catgacatt tggaaccag accaagcaa 
agcaacttg gacataatc agcgaaacg gtggagcaa 
atgtttgaa cacttcccc aacactcct atttttcca 
gcgttgggg aaccatgaa tctatcccg gctgggagt 
tttgcccca ccatgggtc caggacccc gacaggtcc 
attgcctgg ctgtacacc aaggtggcc gaccattgg 
aggaaatgg ttgccggcg agcgccggc aatacggta 
ctccatgga gggttctac tctgtttta atcagacca 
ggattccgc attatttcg ctcaatatg aactactgt 
cacacactt agttggtgg ctcatagtc aacagtacc 
gacccagcc aaggaatta aaatggcta gtgtatgag 
ttgcaagag gctgaaaac aatggcgaa aaagtccac 
ctaattggc cacatcccg cctggaagt tccgactgt 
atgaaggta tggtcccgc aactttaat aaaataatc 
gaacggtat gaaaacacg atccaagcc caattctac 
ggtcacaca catgccgat gaattcgaa gttttgtac 
gacgttgaa gaaagcagc cgaccaatc aatgtggct 
tatctggga ccttcagtt accactttc gaaaatcac 
aatccagct tatcgcata tattatgta gatggtgac 
catgaaaac accaccaga gaaattctc gaccatgag 
acttggact tttgactta gaggaagcc aataagggt 
aatagcgac ccgaaatgg taccgatta tactcggcc 
aaaaatgcc tacaaaatg gactctctc cgcccggaa 
gcttgggca aaactcatt aatgaccta gtcgaaagc 
ccggattta ttcgaacta ttttacaaa cattattat 
agggacagc ccagtgaga ccaacctgt gaccgtaaa 
tgtaagctc caaatattg tgtgacctc aaaagtgga 
agatcccac gtcaaacac cagttatgt cacgactta 
gaattaacc ttcgtgacc cctcaagac atttccgcg 
ttggtcgat gtgaaaact ctgagtgaa cttccctta 
gaaaagctc ctccaagtg aaaagcacg ttccaagaa 
ccggaaact gaggccaag gctgataag aaagtggat 
gggccgtca gcgctggcc ctccagtca aagcgcaaa 
ccgaacgac cgagggggc tatcactac cactatgag 
gaggaaaat tcgaagaaa agtggcaaa aagagtgtg 
caaagtatt ttccaaatt tccgtgacg actctagct 
tttctggcg tttggaggg tatttgttg tgtctgttg 
gtgcaagcc atcaagggc aagactatg atgaatatg 
atggacatg acgacgatg ccgccaact ggggccgct 
ctcttccgg cccaggccc acaaggagg cggcggcgg 
cggcccacg cgaaggccc gtcaagttg caaaggccc 
acgagacaa acctacggg aggaaaccg agggaaaaa 
cgcgaagtt tggcctgaa gccgaccct gagagcatg 
tactacgtc ctcgtgcac ctctctgaa gcctacgtc 
cgttatcac accatcgat tatagaaaa ttcaattac 
acggcgagc tcttatgcg aacatgggt ttcgctcgt 
tcaaaacca ggaggcgaa ccagacatc ccgaaaatg 
gatatgtcc aggaccaaa gcggaaaag gacccgctc 
aagttcggc atcgacaga ctcctgacc agtgatcct 
tgcaaggat aataaaagt gttgtggct aaaccttta 
gcaactgtt ccagtgcct tgttccgac tgtgttacg 
tcgctgttt aggtgttgt cggttgagt ccttcggca 
caggacaat atcggggag tttttcgga cagcatcat 
gggttgagt gtcacaccc acgtccagt atttataca 
gtgcagccc atcaggccc tttgccaca aggccagct 
gtgcgcatc ccggtgcca ccgccgccc ccagcgacg 
tcgccaagc ttaaacatc aaccagaac aacaataag 
aggaagagg tcctggtca agggccgtt tttagcaat 
ttgcaaagg aaaggattg gaaaggcgg tttcagttg 
cagaagtac atcacgaag ccggatagg agacaactt 
gccgctact ttgggtctg acagatgca caagtgaaa 
gtctggttt cagaacagg aggatgaag tggaggcat 
tcgaaagaa gcgggaaaa ggaggcgaa aacagtgca 
gactccacg caggatcaa gatgtgcat atagatgtc 
gatacgatc accgataat gactga
    mRNA sequence
    • >TC031113 RA
      gacgttata acaaatcga aactagaca ataattagg 
aatcaacga tgtgtagtt tgtatttag tggacagcc 
ttgaatgtt gaactacag aataaaata ttaaggttt 
aaataattc ctcttgatt cgcaagtga aaactcgtg 
gagacaaga tggaagcca aactaccgg tcatcctct 
ttctcctag caattaaac agcaaacat ccgaaatag 
aagccactc ctatcgccg aagtgaacg taaacaatt 
tcgaagatg cctgggatt acaaagtgg agccaaaca 
aaatgccag ttttgagcg aatcgccaa aaaatcaca 
atttgcctc cgccagcca aggtcgaaa taccggaga 
agaagttcg tcaagccgg ctccactac cagaacgag 
tcgccaaaa aaatcggag atgctgtta gagcacgag 
tttcttgcc cgatatgta gattaggag tgggcctct 
tacaaaagg aagtcaaaa gaaacacaa gctttgagg 
acatccgaa ccaaatttg tgggacttt gcgtcgcct 
tcaaagtcg aaggtgagg acgtgtgtg ccggaatct 
tcgacgtgt tcggccccg atgtcttac cggcgctcc 
gagtggtgc aaattggac ccgacgaga tctgcgctc 
tagtccttg gcgaagtct gcggtaacg ttaaaatac 
ccctccacg agtggcaag tggaatttc ccgacgtcc 
ccaagccca acatcacga aacgcgacg taccaaaag 
caggtttgc cgactttca aggttttgc aaatttccg 
atacacact ttgaccccg attatgtcg tggggagtg 
tggccaact gcgaggagc ccctctgtt gccgcagca 
ctagcactc cgcccttgg tgggcgaag tggctccag 
ccgggaaat ggggcagtt accaaaagt gtgacgcac 
ccaaagttt tgattgata acatgttga aaagcatag 
cggaagagc atcctgaca tagattatg ttatttgga 
ccggtgatt tgccccctc atgacattt ggaaccaga 
ccaagcaaa gcaacttgg acataatca gcgaaacgg 
tggagcaaa tgtttgaac acttcccca acactccta 
tttttccag cgttgggga accatgaat ctatcccgg 
ctgggagtt ttgccccac catgggtcc aggaccccg 
acaggtcca ttgcctggc tgtacacca aggtggccg 
accattgga ggaaatggt tgccggcga gcgccggca 
atacggtac tccatggag ggttctact ctgttttaa 
tcagaccag gattccgca ttatttcgc tcaatatga 
actactgtc acacactta gttggtggc tcatagtca 
acagtaccg acccagcca aggaattaa aatggctag 
tgtatgagt tgcaagagg ctgaaaaca atggcgaaa 
aagtccacc taattggcc acatcccgc ctggaagtt 
ccgactgta tgaaggtat ggtcccgca actttaata 
aaataatcg aacggtatg aaaacacga tccaagccc 
aattctacg gtcacacac atgccgatg aattcgaag 
ttttgtacg acgttgaag aaagcagcc gaccaatca 
atgtggctt atctgggac cttcagtta ccactttcg 
aaaatcaca atccagctt atcgcatat attatgtag 
atggtgacc atgaaaaca ccaccagag aaattctcg 
accatgaga cttggactt ttgacttag aggaagcca 
ataagggta atagcgacc cgaaatggt accgattat 
actcggcca aaaatgcct acaaaatgg actctctcc 
gcccggaag cttgggcaa aactcatta atgacctag 
tcgaaagcc cggatttat tcgaactat tttacaaac 
attattata gggacagcc cagtgagac caacctgtg 
accgtaaat gtaagctcc aaatattgt gtgacctca 
aaagtggaa gatcccacg tcaaacacc agttatgtc 
acgacttag aattaacct tcgtgaccc ctcaagaca 
tttccgcgt tggtcgatg tgaaaactc tgagtgaac 
ttcccttag aaaagctcc tccaagtga aaagcacgt 
tccaagaac cggaaactg aggccaagg ctgataaga 
aagtggatg ggccgtcag cgctggccc tccagtcaa 
agcgcaaac cgaacgacc gagggggct atcactacc 
actatgagg aggaaaatt cgaagaaaa gtggcaaaa 
agagtgtgc aaagtattt tccaaattt ccgtgacga 
ctctagctt ttctggcgt ttggagggt atttgttgt 
gtctgttgg tgcaagcca tcaagggca agactatga 
tgaatatga tggacatga cgacgatgc cgccaactg 
gggccgctc tcttccggc ccaggccca caaggaggc 
ggcggcggc ggcccacgc gaaggcccg tcaagttgc 
aaaggccca cgagacaaa cctacggga ggaaaccga 
gggaaaaac gcgaagttt ggcctgaag ccgaccctg 
agagcatgt actacgtcc tcgtgcacc tctctgaag 
cctacgtcc gttatcaca ccatcgatt atagaaaat 
tcaattaca cggcgagct cttatgcga acatgggtt 
tcgctcgtt caaaaccag gaggcgaac cagacatcc 
cgaaaatgg atatgtcca ggaccaaag cggaaaagg 
acccgctca agttcggca tcgacagac tcctgacca 
gtgatcctt gcaaggata ataaaagtg ttgtggcta 
aacctttag caactgttc cagtgcctt gttccgact 
gtgttacgt cgctgttta ggtgttgtc ggttgagtc 
cttcggcac aggacaata tcggggagt ttttcggac 
agcatcatg ggttgagtg tcacaccca cgtccagta 
tttatacag tgcagccca tcaggccct ttgccacaa 
ggccagctg tgcgcatcc cggtgccac cgccgcccc 
cagcgacgt cgccaagct taaacatca accagaaca 
acaataaga ggaagaggt cctggtcaa gggccgttt 
ttagcaatt tgcaaagga aaggattgg aaaggcggt 
ttcagttgc agaagtaca tcacgaagc cggatagga 
gacaacttg ccgctactt tgggtctga cagatgcac 
aagtgaaag tctggtttc agaacagga ggatgaagt 
ggaggcatt cgaaagaag cgggaaaag gaggcgaaa 
acagtgcag actccacgc aggatcaag atgtgcata 
tagatgtcg atacgatca ccgataatg actgattct 
tctaacttt gtacataaa tgttttatt attaatact 
ttgaagttt tatttatac ccggcaaag tatacttaa 
gttgcaatt tttttcagc aaagtggca tttattcag 
aagaaaagc aaaattaaa at

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY