Status
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Gene information
General information
TC008351 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8948140..8951979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008351
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
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      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04473

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen bristle pattern lighter - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008351 RA
        MDVKEFTQR FHNLAGVTI GEKRKSLFI LLRNLPSDQ 
        SALITSITP KNHLEQLFK LEVLIRLQS IPHLIEVLK 
        CEEELLPAK LLKNKWFCQ KLASILDED SSNFVNTIL 
        PFTSFVIRM RIIAKLALY GTRMDNVYD DIYKRYGFH 
        IATKVLVGC SESKIRHVL QNGDVVLSP RDLKILYLK 
        HPQVFQFYL KQNGTIDDD SVLSFVARR DKKTFLELM 
        KIFNLRANI GKRLTRHLL NTYKDLIIA QAKTLSTCL 
        RMKTVIKTL SQDDFKRFF FNCLSETFE GLRYASYEM 
        QRKIGKKYY KLLLEGMKE VYNKSLYDY PDYVSEDMM 
        RFMDVEERE KWIELKKKS GTPEADLVK FMRPSLSIP 
        WIKNKINVT SAVTSRDDL ACLLLETCK INNDLHALA 
        EVLEYFCKR HRNDDESVI RNFLYKLSN LYKIEKFEE 
        RHWKCINTL NNILDMKGH NSYFGYDYE LKYVEFLFS 
        NKKLTDEHV YKFLKLTKG VNYWENKVK SEPLKKIFL 
        LKTIELMPE LTDEKKGIH LEDLLIISW IFGWNKKHP 
        NDQINFLSY PRLAESFRK AQNEALNTT EFNLKRRTL 
        SHLYETIIC TKEKSQFRE DTIKNYFLL DYPFYNETI 
        DFWLLKHEP EIVDKHFEK FADHILGTV RPNWQIVNI 
        LKKYSQFHF AERLVKRCV ENVKDGRET LFMFLAALM 
        LTQDFVQII APFYPPNNK IDESSEEER MKGYRAFAV 
        SASLKYATG FVLPTIITY CKGDFLGGA LECLYYHFY 
        HTPENKLSA FINELSSRA VSVRKHAIF LTCQVATVE 
        DTQNLIKKY LETERNISV QKHLFTAAL KYFVKNPSE 
        DFWECVTIC MKNLDKNDN ESFDLLVDV SKIPQKYKA 
        RHVMLVWEM LKVSEDLAP KRTSLLSSI PSDVFKDFP 
        MHFLTEIIT NNFLQCDSK CKNSINDFV ISTILHTSY 
        TSDIINLIF DILTKVKAA SFATLAEEK NARQLVLRF 
        VANFVSTME TDEGDYQIL QLFSDRWHG LFGVGEAIQ 
        EHLKISFAL FNHEAKKDL MKFSNAVAG LLNTLVPKY 
        GIIVVNTFA SELKNSLAS FLHCYGGWN EDFYKFCFN 
        LLENWKDLT IYLLVIKLL PSKEPSQDQ TKALFREIV 
        ERLKQLPDE SIQLLMNLY ITNME
      CDS sequence
      • >TC008351 RA
        atggacgta aaggagttc acacaacgc ttccacaat 
        cttgccggt gtcacaatc ggtgaaaaa aggaagtct 
        ctctttatc cttctgcga aacttacct tcagatcag 
        tctgccctc atcacttcc atcactccc aaaaatcat 
        ttggaacaa ctttttaag ttggaagtt ttaatccga 
        ctacaaagt atcccccat ttaatcgaa gtacttaaa 
        tgcgaagaa gaactcctt cccgcaaaa ttattaaaa 
        aacaagtgg ttttgccaa aaactggca tcgattctt 
        gacgaagac agctcaaat tttgtgaac actattctc 
        ccctttaca tctttcgtc atacgaatg agaataatc 
        gcaaaactt gctctgtac ggcacgaga atggacaat 
        gtgtacgac gatatttac aaacgatat gggttccac 
        atcgcgacg aaggttttg gtaggttgc agcgaatca 
        aaaattcga catgttttg caaaacgga gacgtcgtt 
        ctctcgcct cgcgacctc aaaatattg tatcttaaa 
        cacccacaa gtgtttcaa ttttatttg aaacagaat 
        ggtaccatt gacgatgac tcagttttg agttttgtt 
        gcacgaaga gataagaaa acgtttctc gagctaatg 
        aaaattttc aatttacgg gcaaacatt ggtaaacga 
        ctcaccaga cacttgttg aacacttac aaagattta 
        ataatcgca caagcaaaa acactgagt acgtgtctt 
        cgcatgaaa acagtcatc aaaacacta agtcaagac 
        gattttaag agatttttc ttcaactgt ttgtccgaa 
        actttcgaa ggcttgcgt tatgcctct tacgaaatg 
        cagcgaaaa attgggaaa aaatattac aaactgctt 
        ttggaagga atgaaagag gtttataat aagagtttg 
        tacgattat cccgactac gtcagtgaa gatatgatg 
        cgatttatg gatgttgaa gaaagggaa aagtggatc 
        gaactgaag aagaaaagt ggtaccccg gaagcagat 
        ttggtcaaa tttatgaga ccctctctt tccattcct 
        tggattaaa aataaaatt aatgttacg tccgctgtt 
        acgtcccga gatgacctc gcttgtctg ctccttgaa 
        acgtgcaaa ataaacaat gatttgcac gcgttggcc 
        gaagttttg gaatatttt tgcaagaga cacagaaat 
        gacgatgaa agtgtcata cgcaacttt ctatacaaa 
        cttagcaat ttgtacaaa attgaaaaa tttgaagaa 
        cgccactgg aaatgtatc aacacgcta aataatatt 
        ctggacatg aagggacac aattcttac ttcggttac 
        gattacgag cttaaatat gtcgaattt ttgttctcc 
        aataagaaa ttaaccgac gagcacgtg tataaattt 
        ttgaaacta accaaaggg gttaattac tgggagaac 
        aaagtcaag agtgaacct ttgaaaaaa atatttcta 
        ttaaaaact attgaactc atgcccgaa ctgacggac 
        gagaagaaa ggaatccac ttggaggat ttgttaatc 
        ataagctgg atttttggt tggaacaaa aaacacccc 
        aatgaccag attaatttt ttaagttac cccagattg 
        gcagaatcg tttcgtaag gcacaaaac gaggcactg 
        aataccacc gaattcaat ctaaaacgg agaactttg 
        tcgcactta tacgaaacc atcatatgt acgaaagaa 
        aagtcacaa ttccgagaa gacacaata aaaaattac 
        tttctgcta gattacccg ttttacaat gaaacaatt 
        gatttctgg ttattgaaa catgaacca gaaattgtt 
        gacaaacat ttcgaaaag tttgccgac cacattcta 
        ggcaccgta cgaccaaac tggcaaatt gtgaatatc 
        ctgaaaaaa tattcacaa tttcatttt gctgaaaga 
        ttagtcaaa cgttgcgtc gaaaatgtg aaggatggt 
        cgagagaca ttattcatg ttccttgcg gcgcttatg 
        ctaacgcag gacttcgtc caaataatt gcacctttt 
        taccctcct aacaataaa atcgacgaa agcagcgaa 
        gaagaaaga atgaaaggc taccgagca tttgccgtt 
        tcggcaagt ttgaaatac gccaccggt tttgttctc 
        cctactatc atcacatac tgcaaaggt gattttttg 
        ggtggggct ttagagtgc ctatattac cacttttac 
        cacaccccg gaaaacaag ttgagcgct ttcatcaac 
        gagttatca tcaagagca gtttcggtg cgtaaacac 
        gcaattttc ctaacttgt caagtagca acagttgaa 
        gacacccaa aacttaatc aaaaaatac ctcgagacg 
        gaacgtaac atctcggtc caaaaacac ttatttacc 
        gcagctttg aaatatttc gttaaaaat ccttccgag 
        gatttttgg gagtgtgtc acgatttgt atgaagaat 
        ttggacaaa aacgacaat gagagtttc gatttgttg 
        gtcgatgtg tctaaaata ccacaaaaa tacaaagcg 
        agacacgta atgttggtg tgggaaatg ttgaaagta 
        tcggaggat ttagccccg aaaaggaca agtctatta 
        tcgtcaatc ccttcggat gtcttcaaa gacttcccg 
        atgcatttt ctgacggaa atcatcaca aataatttc 
        ctacaatgt gactccaag tgcaaaaac tcgatcaat 
        gatttcgtt atttccacc atacttcat acgagttac 
        acgagcgat attataaat ttaatcttc gatatttta 
        accaaagtt aaggccgcc tcatttgca actctcgca 
        gaggagaaa aacgcgcgt cagttagtt ttacgtttt 
        gttgcaaat tttgtttca acgatggaa acagacgag 
        ggagattat caaatactt caattattt tccgaccgt 
        tggcacggc ctgtttggg gttggcgaa gcgattcaa 
        gaacactta aaaatctca tttgcgttg tttaatcac 
        gaggcaaag aaagattta atgaaattt agcaacgca 
        gttgctgga cttctcaat actttggtg ccaaaatac 
        ggtattatt gttgtaaac acttttgcg agcgaattg 
        aaaaattct ttggcctcg tttttgcat tgttacggt 
        ggctggaat gaagatttt tacaagttt tgtttcaat 
        ttattggaa aattggaaa gacctaaca atttattta 
        ttggttatt aaacttttg cccagtaaa gagccgtca 
        caagaccaa accaaagcc ttgttcagg gaaattgtt 
        gagcgactc aagcaactt cccgatgaa tctattcaa 
        ctgttgatg aatctttat ataacaaat atggagtag
      mRNA sequence
      • >TC008351 RA
        gtgtaagtt tataaaaaa atcgcattg tacgagtat 
        aaggagtga gctgtaatt ttgaaacta gagtttttt 
        gcaactttt caacaaaaa aaagttgat attatggac 
        gtaaaggag ttcacacaa cgcttccac aatcttgcc 
        ggtgtcaca atcggtgaa aaaaggaag tctctcttt 
        atccttctg cgaaactta ccttcagat cagtctgcc 
        ctcatcact tccatcact cccaaaaat catttggaa 
        caacttttt aagttggaa gttttaatc cgactacaa 
        agtatcccc catttaatc gaagtactt aaatgcgaa 
        gaagaactc cttcccgca aaattatta aaaaacaag 
        tggttttgc caaaaactg gcatcgatt cttgacgaa 
        gacagctca aattttgtg aacactatt ctccccttt 
        acatctttc gtcatacga atgagaata atcgcaaaa 
        cttgctctg tacggcacg agaatggac aatgtgtac 
        gacgatatt tacaaacga tatgggttc cacatcgcg 
        acgaaggtt ttggtaggt tgcagcgaa tcaaaaatt 
        cgacatgtt ttgcaaaac ggagacgtc gttctctcg 
        cctcgcgac ctcaaaata ttgtatctt aaacaccca 
        caagtgttt caattttat ttgaaacag aatggtacc 
        attgacgat gactcagtt ttgagtttt gttgcacga 
        agagataag aaaacgttt ctcgagcta atgaaaatt 
        ttcaattta cgggcaaac attggtaaa cgactcacc 
        agacacttg ttgaacact tacaaagat ttaataatc 
        gcacaagca aaaacactg agtacgtgt cttcgcatg 
        aaaacagtc atcaaaaca ctaagtcaa gacgatttt 
        aagagattt ttcttcaac tgtttgtcc gaaactttc 
        gaaggcttg cgttatgcc tcttacgaa atgcagcga 
        aaaattggg aaaaaatat tacaaactg cttttggaa 
        ggaatgaaa gaggtttat aataagagt ttgtacgat 
        tatcccgac tacgtcagt gaagatatg atgcgattt 
        atggatgtt gaagaaagg gaaaagtgg atcgaactg 
        aagaagaaa agtggtacc ccggaagca gatttggtc 
        aaatttatg agaccctct ctttccatt ccttggatt 
        aaaaataaa attaatgtt acgtccgct gttacgtcc 
        cgagatgac ctcgcttgt ctgctcctt gaaacgtgc 
        aaaataaac aatgatttg cacgcgttg gccgaagtt 
        ttggaatat ttttgcaag agacacaga aatgacgat 
        gaaagtgtc atacgcaac tttctatac aaacttagc 
        aatttgtac aaaattgaa aaatttgaa gaacgccac 
        tggaaatgt atcaacacg ctaaataat attctggac 
        atgaaggga cacaattct tacttcggt tacgattac 
        gagcttaaa tatgtcgaa tttttgttc tccaataag 
        aaattaacc gacgagcac gtgtataaa tttttgaaa 
        ctaaccaaa ggggttaat tactgggag aacaaagtc 
        aagagtgaa cctttgaaa aaaatattt ctattaaaa 
        actattgaa ctcatgccc gaactgacg gacgagaag 
        aaaggaatc cacttggag gatttgtta atcataagc 
        tggattttt ggttggaac aaaaaacac cccaatgac 
        cagattaat tttttaagt taccccaga ttggcagaa 
        tcgtttcgt aaggcacaa aacgaggca ctgaatacc 
        accgaattc aatctaaaa cggagaact ttgtcgcac 
        ttatacgaa accatcata tgtacgaaa gaaaagtca 
        caattccga gaagacaca ataaaaaat tactttctg 
        ctagattac ccgttttac aatgaaaca attgatttc 
        tggttattg aaacatgaa ccagaaatt gttgacaaa 
        catttcgaa aagtttgcc gaccacatt ctaggcacc 
        gtacgacca aactggcaa attgtgaat atcctgaaa 
        aaatattca caatttcat tttgctgaa agattagtc 
        aaacgttgc gtcgaaaat gtgaaggat ggtcgagag 
        acattattc atgttcctt gcggcgctt atgctaacg 
        caggacttc gtccaaata attgcacct ttttaccct 
        cctaacaat aaaatcgac gaaagcagc gaagaagaa 
        agaatgaaa ggctaccga gcatttgcc gtttcggca 
        agtttgaaa tacgccacc ggttttgtt ctccctact 
        atcatcaca tactgcaaa ggtgatttt ttgggtggg 
        gctttagag tgcctatat taccacttt taccacacc 
        ccggaaaac aagttgagc gctttcatc aacgagtta 
        tcatcaaga gcagtttcg gtgcgtaaa cacgcaatt 
        ttcctaact tgtcaagta gcaacagtt gaagacacc 
        caaaactta atcaaaaaa tacctcgag acggaacgt 
        aacatctcg gtccaaaaa cacttattt accgcagct 
        ttgaaatat ttcgttaaa aatccttcc gaggatttt 
        tgggagtgt gtcacgatt tgtatgaag aatttggac 
        aaaaacgac aatgagagt ttcgatttg ttggtcgat 
        gtgtctaaa ataccacaa aaatacaaa gcgagacac 
        gtaatgttg gtgtgggaa atgttgaaa gtatcggag 
        gatttagcc ccgaaaagg acaagtcta ttatcgtca 
        atcccttcg gatgtcttc aaagacttc ccgatgcat 
        tttctgacg gaaatcatc acaaataat ttcctacaa 
        tgtgactcc aagtgcaaa aactcgatc aatgatttc 
        gttatttcc accatactt catacgagt tacacgagc 
        gatattata aatttaatc ttcgatatt ttaaccaaa 
        gttaaggcc gcctcattt gcaactctc gcagaggag 
        aaaaacgcg cgtcagtta gttttacgt tttgttgca 
        aattttgtt tcaacgatg gaaacagac gagggagat 
        tatcaaata cttcaatta ttttccgac cgttggcac 
        ggcctgttt ggggttggc gaagcgatt caagaacac 
        ttaaaaatc tcatttgcg ttgtttaat cacgaggca 
        aagaaagat ttaatgaaa tttagcaac gcagttgct 
        ggacttctc aatactttg gtgccaaaa tacggtatt 
        attgttgta aacactttt gcgagcgaa ttgaaaaat 
        tctttggcc tcgtttttg cattgttac ggtggctgg 
        aatgaagat ttttacaag ttttgtttc aatttattg 
        gaaaattgg aaagaccta acaatttat ttattggtt 
        attaaactt ttgcccagt aaagagccg tcacaagac 
        caaaccaaa gccttgttc agggaaatt gttgagcga 
        ctcaagcaa cttcccgat gaatctatt caactgttg 
        atgaatctt tatataaca aatatggag tagaactta 
        taatttgtg cttttgatg gtattttca taacattgt 
        taaaggacg ttgaataaa gtaactaaa tacaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY