| General information | |||
| TC008348 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 8898610..8900537 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008348 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04472 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
- >TC008348 RA
MKLHTFLTT YLTINFASC NTIDSGNDY DFIIVGAGS AGSVIASRL SENLIWKIL LLEAGDEGN LISSIPTAV SLLPFTKYN WGHFMEVQP NLAQSYNDN RMPWHKGRG LGGTSLINY MIYTRGNRF NYDQWAAQG NPGWSYADV LPYFIKSEN CSVKNADYA FHGVDGYLG ISEPFQTKI TDVFLKGLH ELGLPFIDY NSNKTLGAS PIQANIFQG RRHTSADAF LKPVKHRFN LHIKTRAFA RKVLIDEKT KHAFGVEYE VSGKIFKAM ARKEVILSA GVINSPQLL MLSGIGPKQ ELGQLGISV LKDLQVGRN LQDNLAFLG LNFVTPEDV TLRFSKFVN LVSIYEVFE SRTGPWVGA GGAQAIAYI KTDESEELG PVPDMELLL IGGSLSTDY GLILRTGMN IRDDVYNSL FAPTEGKNS FMIFLSHLT PKSKGYIKL RSADPHDYP LMYGNYFTD PGNKDINTF LAAVRYVQK LIQTETFKK FKITLIDNP VPGCTHHQY DSDDYWRCF LRSLIQTFN HQVGTAKMG PKNDPDAVV NHKLEVYGV KGLRVADCS VIPFALSAH TNAPAMMVG EKAADIIKN AWKDNLW
- >TC008348 RA
atgaagctt cacaccttt ttaacaact tacctcacc attaatttt gcatcatgt aacaccatt gattcaggc aacgactac gattttata attgttggt gctggctca gctggaagt gttatagcg agccgatta tcagaaaat ctcatttgg aaaattctt cttctcgaa gccggagac gaaggcaac ctcatctcg tcaatacca acagcagtg tctctccta cctttcact aaatacaac tggggacac tttatggaa gtccaacca aacttggcc cagtcctac aacgacaat cgaatgcct tggcacaag ggccgaggc ttaggcggc acttccctt atcaactac atgatatac acccgtgga aataggttc aattacgac cagtgggcc gcacaaggc aacccaggt tggtcctac gccgacgtt ctaccctat tttatcaaa tcagaaaac tgcagcgtg aaaaacgcg gattatgcc tttcacggt gtggacggt tatttgggt atctctgag ccgtttcaa actaaaatc accgatgtt ttcctcaaa gggctccac gagctgggg ctaccattt attgattat aactcaaac aaaacttta ggagcttcg ccgattcaa gcgaatatt tttcaaggc aggagacac acaagtgcc gatgcgttc ctcaaaccg gtgaaacac aggtttaat cttcacatc aaaacgaga gctttcgct cggaaagtc ctaattgat gagaagaca aaacacgcg tttggtgtt gaatacgaa gtttcgggc aaaatattt aaagccatg gctcggaaa gaagtgatt ttgtcagcc ggtgttata aattcgccc cagctgctt atgctttca gggattggg ccgaagcaa gaattagga caattggga atttctgtt ttaaaagac ctccaagtt gggagaaat ctgcaagac aatttggct tttctagga ctcaatttt gtgacgcct gaagacgtt acattgcgt ttctcaaaa tttgtcaac cttgttagt atctacgaa gtgtttgag agtaggacg gggccatgg gttggtgca ggaggtgcc caagctatc gcctatata aagacggac gaatccgag gaattaggg cctgtgcct gatatggaa ttattgcta atcggagga tctttatcg accgattat ggcctaatt ctccggaca ggaatgaat attagagac gatgtttac aattcgctt tttgcccca actgaaggg aaaaattcc tttatgatc ttcctctca cacttgacc ccgaaatcg aaaggatat ataaaactc cggtcggct gaccctcac gactacccc ctcatgtat ggaaattat ttcacagat ccgggaaat aaagacata aatacgttt ctggctgcg gttagatac gtgcaaaag ctcattcag acggaaact ttcaaaaaa ttcaaaata actcttatc gataatccg gttcctggc tgcacacat caccagtac gatagtgat gattattgg aggtgtttc ctccgatcg cttatacaa acttttaat catcaagtc gggacggca aaaatgggg cccaagaat gaccccgat gctgtggtg aatcacaaa ttggaagtt tacggggtt aagggattg cgagtggct gattgtagt gttattcct tttgcactg agtgcacac actaatgcc ccagctatg atggtgggc gaaaaggca gccgatatt atcaaaaat gcgtggaag gataattta tggtaa
- >TC008348 RA
ccatttcac aaaaatcca aattagttc caccaacac atcatgaag cttcacacc tttttaaca acttacctc accattaat tttgcatca tgtaacacc attgattca ggcaacgac tacgatttt ataattgtt ggtgctggc tcagctgga agtgttata gcgagccga ttatcagaa aatctcatt tggaaaatt cttcttctc gaagccgga gacgaaggc aacctcatc tcgtcaata ccaacagca gtgtctctc ctacctttc actaaatac aactgggga cactttatg gaagtccaa ccaaacttg gcccagtcc tacaacgac aatcgaatg ccttggcac aagggccga ggcttaggc ggcacttcc cttatcaac tacatgata tacacccgt ggaaatagg ttcaattac gaccagtgg gccgcacaa ggcaaccca ggttggtcc tacgccgac gttctaccc tattttatc aaatcagaa aactgcagc gtgaaaaac gcggattat gcctttcac ggtgtggac ggttatttg ggtatctct gagccgttt caaactaaa atcaccgat gttttcctc aaagggctc cacgagctg gggctacca tttattgat tataactca aacaaaact ttaggagct tcgccgatt caagcgaat atttttcaa ggcaggaga cacacaagt gccgatgcg ttcctcaaa ccggtgaaa cacaggttt aatcttcac atcaaaacg agagctttc gctcggaaa gtcctaatt gatgagaag acaaaacac gcgtttggt gttgaatac gaagtttcg ggcaaaata tttaaagcc atggctcgg aaagaagtg attttgtca gccggtgtt ataaattcg ccccagctg cttatgctt tcagggatt gggccgaag caagaatta ggacaattg ggaatttct gttttaaaa gacctccaa gttgggaga aatctgcaa gacaatttg gcttttcta ggactcaat tttgtgacg cctgaagac gttacattg cgtttctca aaatttgtc aaccttgtt agtatctac gaagtgttt gagagtagg acggggcca tgggttggt gcaggaggt gcccaagct atcgcctat ataaagacg gacgaatcc gaggaatta gggcctgtg cctgatatg gaattattg ctaatcgga ggatcttta tcgaccgat tatggccta attctccgg acaggaatg aatattaga gacgatgtt tacaattcg ctttttgcc ccaactgaa gggaaaaat tcctttatg atcttcctc tcacacttg accccgaaa tcgaaagga tatataaaa ctccggtcg gctgaccct cacgactac cccctcatg tatggaaat tatttcaca gatccggga aataaagac ataaatacg tttctggct gcggttaga tacgtgcaa aagctcatt cagacggaa actttcaaa aaattcaaa ataactctt atcgataat ccggttcct ggctgcaca catcaccag tacgatagt gatgattat tggaggtgt ttcctccga tcgcttata caaactttt aatcatcaa gtcgggacg gcaaaaatg gggcccaag aatgacccc gatgctgtg gtgaatcac aaattggaa gtttacggg gttaaggga ttgcgagtg gctgattgt agtgttatt ccttttgca ctgagtgca cacactaat gccccagct atgatggtg ggcgaaaag gcagccgat attatcaaa aatgcgtgg aaggataat ttatggtaa aatgactca gagacttga aatataaat agattgtac cagtggttt aataaaaaa cattttcaa ta
