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Gene information
General information
TC008340 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8752830..8756217
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008340
      iBeetle screen iB_04471

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      procephalic appendages not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      gnathal appendages random partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008340 RA
        MKCLLVAIF LAHSAKCME SVYLKRSEI EDQESSASG 
        YAYQLHSGG PLSYAALPA AGGLNHFAY PPLGLGQYS 
        QEIPLQLPE YKPLAPVGV VPLQTYGLQ GQPAAYGIH 
        YPLQTHQIS PAPIPVPVS VSESSKFAK GGGSSFSDQ 
        NQNSHGEKG STGYKTVQE YDKGAQGKH DNQGHKEHY 
        SKEGGHKAA HHDSGSHYN QKQEAAKGS EGSSFGQSS 
        SHKKGSKTT GFHRVHHKD EYKKDHTFY DESDASGHF 
        NKHGDFDSH HAAKKGGFE KGGHEDGAY QAGEQGVKG 
        FVDNGKYVG QNSGFKGEQ GKEGHYKDF QEYAKKGEQ 
        QQGKEQGFY KKDVLQALQ
      CDS sequence
      • >TC008340 RA
        atgaagtgt ctactggtg gcaatcttt ctcgcacac 
        agtgcaaaa tgcatggag agcgtctat ttgaaacgc 
        tctgaaatt gaagaccaa gagtcctcc gcttcgggt 
        tacgcgtat cagcttcac tccggaggg ccgttaagt 
        tacgccgcc ctcccggca gcagggggc ctaaatcat 
        ttcgcttat cctccgctc ggcttagga caatattcg 
        caagaaata cccctgcaa ttaccggag tacaagccg 
        ctagcaccc gtcggggtg gtgcccctc cagacctac 
        ggcctgcaa gggcaaccc gccgcctac ggcatacac 
        taccccctg cagacgcac cagatcagt ccggccccc 
        attccggtg ccggtgtct gtgtccgaa tcgtcaaaa 
        ttcgcaaaa ggcggtggc tcaagtttc tccgaccag 
        aaccagaac agtcacggg gaaaaaggc tccactggc 
        tacaagacc gtgcaagaa tacgacaag ggggcacaa 
        ggcaaacac gacaaccaa ggccacaaa gagcattac 
        agtaaagag ggcgggcat aaagcggcc catcacgac 
        tccggttct cattacaac cagaaacag gaagcggcc 
        aaaggctca gaaggatcg tctttcggg caatcatcg 
        agtcataag aaaggctca aagacaaca ggtttccac 
        cgcgtccat cataaagac gagtataag aaggatcat 
        acgttttac gacgaatcc gatgctagc ggacacttc 
        aataaacac ggcgatttc gattcccat catgcggcc 
        aaaaagggc ggatttgag aaaggtgga cacgaagat 
        ggggcttat caggctggg gagcaagga gttaaagga 
        tttgtggat aacgggaaa tatgtcggc cagaatagt 
        ggatttaag ggagaacaa gggaaggaa ggacattat 
        aaggacttc caggagtac gccaagaag ggggagcag 
        cagcaggga aaggagcaa ggattttat aagaaggat 
        gttctgcaa gccttgcag tga
      mRNA sequence
      • >TC008340 RA
        catcagtgg tgtcccgtt gcagagatg aagtgtcta 
        ctggtggca atctttctc gcacacagt gcaaaatgc 
        atggagagc gtctatttg aaacgctct gaaattgaa 
        gaccaagag tcctccgct tcgggttac gcgtatcag 
        cttcactcc ggagggccg ttaagttac gccgccctc 
        ccggcagca gggggccta aatcatttc gcttatcct 
        ccgctcggc ttaggacaa tattcgcaa gaaataccc 
        ctgcaatta ccggagtac aagccgcta gcacccgtc 
        ggggtggtg cccctccag acctacggc ctgcaaggg 
        caacccgcc gcctacggc atacactac cccctgcag 
        acgcaccag atcagtccg gcccccatt ccggtgccg 
        gtgtctgtg tccgaatcg tcaaaattc gcaaaaggc 
        ggtggctca agtttctcc gaccagaac cagaacagt 
        cacggggaa aaaggctcc actggctac aagaccgtg 
        caagaatac gacaagggg gcacaaggc aaacacgac 
        aaccaaggc cacaaagag cattacagt aaagagggc 
        gggcataaa gcggcccat cacgactcc ggttctcat 
        tacaaccag aaacaggaa gcggccaaa ggctcagaa 
        ggatcgtct ttcgggcaa tcatcgagt cataagaaa 
        ggctcaaag acaacaggt ttccaccgc gtccatcat 
        aaagacgag tataagaag gatcatacg ttttacgac 
        gaatccgat gctagcgga cacttcaat aaacacggc 
        gatttcgat tcccatcat gcggccaaa aagggcgga 
        tttgagaaa ggtggacac gaagatggg gcttatcag 
        gctggggag caaggagtt aaaggattt gtggataac 
        gggaaatat gtcggccag aatagtgga tttaaggga 
        gaacaaggg aaggaagga cattataag gacttccag 
        gagtacgcc aagaagggg gagcagcag cagggaaag 
        gagcaagga ttttataag aaggatgtt ctgcaagcc 
        ttgcagtga cgcgtgggc ggaggactg ttctagtgc 
        gaaggcggt tattattta tttaatctt tgttaactt 
        attgcataa taaagcttt gtgatattc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY