| General information | |||
| TC008337 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 8696130..8704868 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008337 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04470 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC008337 RA
MVVDVPLRS FHHKMQPTG MSPRELSEN DDWATSLVL DPHLGFTTH KMNLRYRPI KTSTAELKS IVEEFQKTQ NYEKAYNSI AKGEWMPKI KSKIQQNKL KEHIYRYLR VFDKESGFV IEPCYRYSL EGQKGAKIS ATKKWYKND KIECLVGCI AELTEEEEK QLLHPGKND FSVMYSCRK NCAQLWLGP AAYINHDCR ANCKFVATG RDTACVKVL RDIEAGEEI TCFYGEDFF GDKNCYCEC ETCERRGMG AFAKESDKK EENGYRLRE TDNRINRTK QKITCNNNK EEQFGGNRG EEKIVAPLS MKELRQKGL TKYDAELLI AQGCKFSDI GEFPRKNRL SVSSAGNDN NNKKSEVKK SVINEEPQA LVSSRTSRL QRRQARQAN QELMQTATH NVSRSNLLD DLQSNASSC MSTCSDQDP VCDATQQNK KFNDKHESG KGVPQITDR PPTPVQTNE NGITLRSHK CLNVEKSES VMPKLEPQV VCNQNGIKH LSKRGTARV KTRHSSHLT KLNNNITAN NDKDVYEFE DEDSKTDSL CLRRNRTKE TNGDNVIKP EIDNEDSRE KSDDNQNNW HEVKTEPVF VPETTQVEK TPEKCGRLK LTLRMKRSP VLDEVIESG NSLSEDSFE PEYEVLRVE GVEYTPFSH RKKRHKSKD RKRDRKLKQ SEEVTHLPM KRLRLIFGN ESHTIDIPS TSSNCHT
- >TC008337 RA
atggttgtg gatgtacct ttgaggagt tttcatcac aaaatgcag cctactggc atgagtcct cgtgaatta tccgaaaac gatgactgg gctacatcc ctggtgcta gacccacat ctcggattc accacacat aaaatgaac cttcggtac aggcccatc aaaaccagc acagctgaa cttaaaagt attgtggaa gagtttcaa aaaactcag aattacgag aaagcctac aacagtatt gccaaggga gaatggatg cccaaaatc aagtccaaa atccagcag aacaaactc aaggagcat atttatcgc tatctaagg gtattcgac aaagagtcg ggtttcgtg atcgaacct tgttatcgt tactcgctc gagggtcaa aagggcgct aaaatctca gcgacgaaa aaatggtac aaaaacgac aaaatcgaa tgtttggtc ggttgcatc gccgaattg accgaagaa gaggaaaag cagcttctt catccggga aaaaacgac ttttccgtg atgtacagt tgccgaaag aattgtgca caactctgg ttgggccct gctgcgtat atcaatcac gattgcagg gcaaattgt aaattcgtg gctactggt agagacacg gcgtgtgtt aaagttttg cgcgacatt gaagcgggt gaagagatt acgtgtttc tacggtgaa gatttcttc ggtgataaa aattgctat tgcgagtgc gaaacgtgt gaaagacgc ggaatgggc gcttttgcg aaagaaagc gacaaaaag gaggagaac ggttacaga ttgcgcgaa accgataac cgaattaac cggaccaag cagaaaatc acgtgtaat aataacaaa gaggaacag tttggtggt aatcgcggc gaggagaaa atcgtggcg ccgttgagt atgaaagaa ttgagacag aaggggttg acgaagtac gatgctgaa cttttgata gcgcagggg tgcaagttt tcggatatt ggggagttt ccgaggaag aaccggtta agtgtgtcg agtgcgggt aatgacaat aataacaag aaatctgaa gttaaaaaa tctgtgata aatgaagaa ccgcaagcc ttggttagt tcgaggact tcgagactt caaagaaga caggcgaga caagcaaat caggagctt atgcaaact gccactcat aatgtgtcg agatccaat cttttagat gacttgcag agtaacgcg agtagttgt atgagcact tgtagcgat caagatcca gtttgtgat gccacacag cagaataag aagtttaat gacaaacac gagagcgga aaaggggtg ccgcagatt acagatcgc cccccgact ccggtgcaa acaaacgag aacggcatc acattgcgt agccacaaa tgcctcaat gtcgaaaaa tctgagagt gtaatgccg aaattggaa cctcaggtc gtctgtaac caaaacggt attaaacac ttatcaaaa cgtggcaca gctcgcgtg aaaacccgt cattcatcg catctcacc aagttgaat aacaatatc actgccaat aacgataaa gacgtatac gagttcgag gacgaagac tcgaagacg gactcctta tgtctgcga aggaacaga acgaaagaa acgaacggt gataatgtg ataaaaccc gaaattgat aacgaggat tcgagggaa aagtctgac gataatcag aacaattgg cacgaggtt aaaaccgaa ccggttttc gtgccggag acgacccaa gtggagaaa acaccggaa aagtgcgga cggttgaaa ttgacgttg aggatgaaa cggagcccg gtcttggat gaagttatc gagtccggg aatagtctg agtgaagac agtttcgaa ccggagtat gaagtgttg agagtggag ggcgtagag tatacacct ttttcacac cggaaaaag agacataaa tcgaaggac aggaaaagg gataggaaa ttaaaacaa agtgaagag gtgacccat ttaccaatg aagagatta cggttaata tttgggaat gagagtcac acgatcgat atcccgtcc actagttca aattgtcat acatga
- >TC008337 RA
gctctgtaa attcggttt gttcccgtg ctaacttat gtttatgat gaaaaattc ctgaaaccc catttttgt ccccgctag acccgatca ggctaacat tgttgtatt aatcaaaaa taagtatac gggaaagcg actgccatg attgattcg aagcgtcgt tgcaaagac gtccaagta gaaaagttt ttatattat gtgggttat ttaggagga aatcagcag tgaagtggt aataaaact ttgttttaa gcctgtaga ttgtatatt tagggtcag ttgtatcgg actaagtca tgttaatat tgaaatcgc gattttgac gtgtaaggt tcaatggct acaccgtgt acgccatta tttttcggt cgccaaaat cgcgttttt ttcgcctta atccggcgc tttgacggc gaaatgtgc cccatttcg cgtcggttt ttgcaacta taaattgtg aggtcggaa aaagcctaa ttaaaaaat cgagtggga gtgtcataa tttgacatt taaaaaaac taaaatttt ctttgttgt cggcctgtc cgttgacct tggattcca acctatttg tttacatgc tggatggat ggaagttct tcgattttc tgaattatt gcagtattc ataatggtt gtggatgta cctttgagg agttttcat cacaaaatg cagcctact ggcatgagt cctcgtgaa ttatccgaa aacgatgac tgggctaca tccctggtg ctagaccca catctcgga ttcaccaca cataaaatg aaccttcgg tacaggccc atcaaaacc agcacagct gaacttaaa agtattgtg gaagagttt caaaaaact cagaattac gagaaagcc tacaacagt attgccaag ggagaatgg atgcccaaa atcaagtcc aaaatccag cagaacaaa ctcaaggag catatttat cgctatcta agggtattc gacaaagag tcgggtttc gtgatcgaa ccttgttat cgttactcg ctcgagggt caaaagggc gctaaaatc tcagcgacg aaaaaatgg tacaaaaac gacaaaatc gaatgtttg gtcggttgc atcgccgaa ttgaccgaa gaagaggaa aagcagctt cttcatccg ggaaaaaac gacttttcc gtgatgtac agttgccga aagaattgt gcacaactc tggttgggc cctgctgcg tatatcaat cacgattgc agggcaaat tgtaaattc gtggctact ggtagagac acggcgtgt gttaaagtt ttgcgcgac attgaagcg ggtgaagag attacgtgt ttctacggt gaagatttc ttcggtgat aaaaattgc tattgcgag tgcgaaacg tgtgaaaga cgcggaatg ggcgctttt gcgaaagaa agcgacaaa aaggaggag aacggttac agattgcgc gaaaccgat aaccgaatt aaccggacc aagcagaaa atcacgtgt aataataac aaagaggaa cagtttggt ggtaatcgc ggcgaggag aaaatcgtg gcgccgttg agtatgaaa gaattgaga cagaagggg ttgacgaag tacgatgct gaacttttg atagcgcag gggtgcaag ttttcggat attggggag tttccgagg aagaaccgg ttaagtgtg tcgagtgcg ggtaatgac aataataac aagaaatct gaagttaaa aaatctgtg ataaatgaa gaaccgcaa gccttggtt agttcgagg acttcgaga cttcaaaga agacaggcg agacaagca aatcaggag cttatgcaa actgccact cataatgtg tcgagatcc aatctttta gatgacttg cagagtaac gcgagtagt tgtatgagc acttgtagc gatcaagat ccagtttgt gatgccaca cagcagaat aagaagttt aatgacaaa cacgagagc ggaaaaggg gtgccgcag attacagat cgccccccg actccggtg caaacaaac gagaacggc atcacattg cgtagccac aaatgcctc aatgtcgaa aaatctgag agtgtaatg ccgaaattg gaacctcag gtcgtctgt aaccaaaac ggtattaaa cacttatca aaacgtggc acagctcgc gtgaaaacc cgtcattca tcgcatctc accaagttg aataacaat atcactgcc aataacgat aaagacgta tacgagttc gaggacgaa gactcgaag acggactcc ttatgtctg cgaaggaac agaacgaaa gaaacgaac ggtgataat gtgataaaa cccgaaatt gataacgag gattcgagg gaaaagtct gacgataat cagaacaat tggcacgag gttaaaacc gaaccggtt ttcgtgccg gagacgacc caagtggag aaaacaccg gaaaagtgc ggacggttg aaattgacg ttgaggatg aaacggagc ccggtcttg gatgaagtt atcgagtcc gggaatagt ctgagtgaa gacagtttc gaaccggag tatgaagtg ttgagagtg gagggcgta gagtataca cctttttca caccggaaa aagagacat aaatcgaag gacaggaaa agggatagg aaattaaaa caaagtgaa gaggtgacc catttacca atgaagaga ttacggtta atatttggg aatgagagt cacacgatc gatatcccg tccactagt tcaaattgt catacatga ctttttccg ttctgatgt aacacgatt cctttgtta gttttattt ttgtaattg tacagcagg acctttttt atttattta ttttactaa ctttctaac gtttaagtg aattttgtc ttttggatg ttagggttg tggcgcact cgatcttcg ctaagattt acaagtctt ttatttatt tgggtgttt taggcgtga attgatctc atcaggtgt ttataaaac aatatgaca cacgagaag tgtgataca acaattagg tttatcgtg aaactttgc ataaaaatc gttacttgt gtgcacgaa tgattgtgt gaacgtaat taaaagact tgtatattt atgaaaaat gctagattg agagtcgtc attcataaa atagaccgc tgtgattca gaatgttag cgttagtga gaccaaact actttggtc ggaagctta tttttaata aatcgtgta aaatgtaat attttattt gtggtattc tttcagaac cgaccggat gcgttgtat tagcggtta ggggattct aacaaataa aatttcagg acttgttgc cttcgttgt gcagtaata tacaaaaaa ccattccat ttaaacaga tcattattt attttattt atgtacaag gacacagat cttggtata tttttgttg aatgggtgc acacacctt agattagat tttttccat agttttatt ttcacaagc atttatcga aatggtggg ttgagtgag cgccgtacg ctgttctat tacgttttt agataaatt cttgttgaa atttactgt tgaaatttt atgatgtct gtggagaag tgtatgcat ctgtgaata atgtaattc atcgagatt ggtgcatat tgcacggta tttgtaaat atatttgta atatgtacg ataagaatt atgcattac ttaaattta cctcatgtt tatatgaaa acacatcat cgatcattt cttacattg tttcggcgt tgatcccct taatgagtt cctaatgct cccacaatt agttcatct ttgcgtgaa tgcagtgca tgtgtgcgt ggatgtgtt atgtattaa tgtaagaaa tgataacaa tgtactaaa ctgaataaa gttttataa aacca
