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Gene information
General information
TC008337 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8696130..8704868
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    iBeetle screen iB_04470

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC008337 RA
    CDS sequence
    • >TC008337 RA
      atggttgtg gatgtacct ttgaggagt tttcatcac 
      aaaatgcag cctactggc atgagtcct cgtgaatta 
      tccgaaaac gatgactgg gctacatcc ctggtgcta 
      gacccacat ctcggattc accacacat aaaatgaac 
      cttcggtac aggcccatc aaaaccagc acagctgaa 
      cttaaaagt attgtggaa gagtttcaa aaaactcag 
      aattacgag aaagcctac aacagtatt gccaaggga 
      gaatggatg cccaaaatc aagtccaaa atccagcag 
      aacaaactc aaggagcat atttatcgc tatctaagg 
      gtattcgac aaagagtcg ggtttcgtg atcgaacct 
      tgttatcgt tactcgctc gagggtcaa aagggcgct 
      aaaatctca gcgacgaaa aaatggtac aaaaacgac 
      aaaatcgaa tgtttggtc ggttgcatc gccgaattg 
      accgaagaa gaggaaaag cagcttctt catccggga 
      aaaaacgac ttttccgtg atgtacagt tgccgaaag 
      aattgtgca caactctgg ttgggccct gctgcgtat 
      atcaatcac gattgcagg gcaaattgt aaattcgtg 
      gctactggt agagacacg gcgtgtgtt aaagttttg 
      cgcgacatt gaagcgggt gaagagatt acgtgtttc 
      tacggtgaa gatttcttc ggtgataaa aattgctat 
      tgcgagtgc gaaacgtgt gaaagacgc ggaatgggc 
      gcttttgcg aaagaaagc gacaaaaag gaggagaac 
      ggttacaga ttgcgcgaa accgataac cgaattaac 
      cggaccaag cagaaaatc acgtgtaat aataacaaa 
      gaggaacag tttggtggt aatcgcggc gaggagaaa 
      atcgtggcg ccgttgagt atgaaagaa ttgagacag 
      aaggggttg acgaagtac gatgctgaa cttttgata 
      gcgcagggg tgcaagttt tcggatatt ggggagttt 
      ccgaggaag aaccggtta agtgtgtcg agtgcgggt 
      aatgacaat aataacaag aaatctgaa gttaaaaaa 
      tctgtgata aatgaagaa ccgcaagcc ttggttagt 
      tcgaggact tcgagactt caaagaaga caggcgaga 
      caagcaaat caggagctt atgcaaact gccactcat 
      aatgtgtcg agatccaat cttttagat gacttgcag 
      agtaacgcg agtagttgt atgagcact tgtagcgat 
      caagatcca gtttgtgat gccacacag cagaataag 
      aagtttaat gacaaacac gagagcgga aaaggggtg 
      ccgcagatt acagatcgc cccccgact ccggtgcaa 
      acaaacgag aacggcatc acattgcgt agccacaaa 
      tgcctcaat gtcgaaaaa tctgagagt gtaatgccg 
      aaattggaa cctcaggtc gtctgtaac caaaacggt 
      attaaacac ttatcaaaa cgtggcaca gctcgcgtg 
      aaaacccgt cattcatcg catctcacc aagttgaat 
      aacaatatc actgccaat aacgataaa gacgtatac 
      gagttcgag gacgaagac tcgaagacg gactcctta 
      tgtctgcga aggaacaga acgaaagaa acgaacggt 
      gataatgtg ataaaaccc gaaattgat aacgaggat 
      tcgagggaa aagtctgac gataatcag aacaattgg 
      cacgaggtt aaaaccgaa ccggttttc gtgccggag 
      acgacccaa gtggagaaa acaccggaa aagtgcgga 
      cggttgaaa ttgacgttg aggatgaaa cggagcccg 
      gtcttggat gaagttatc gagtccggg aatagtctg 
      agtgaagac agtttcgaa ccggagtat gaagtgttg 
      agagtggag ggcgtagag tatacacct ttttcacac 
      cggaaaaag agacataaa tcgaaggac aggaaaagg 
      gataggaaa ttaaaacaa agtgaagag gtgacccat 
      ttaccaatg aagagatta cggttaata tttgggaat 
      gagagtcac acgatcgat atcccgtcc actagttca 
      aattgtcat acatga
    mRNA sequence
    • >TC008337 RA
      gctctgtaa attcggttt gttcccgtg ctaacttat 
      gtttatgat gaaaaattc ctgaaaccc catttttgt 
      ccccgctag acccgatca ggctaacat tgttgtatt 
      aatcaaaaa taagtatac gggaaagcg actgccatg 
      attgattcg aagcgtcgt tgcaaagac gtccaagta 
      gaaaagttt ttatattat gtgggttat ttaggagga 
      aatcagcag tgaagtggt aataaaact ttgttttaa 
      gcctgtaga ttgtatatt tagggtcag ttgtatcgg 
      actaagtca tgttaatat tgaaatcgc gattttgac 
      gtgtaaggt tcaatggct acaccgtgt acgccatta 
      tttttcggt cgccaaaat cgcgttttt ttcgcctta 
      atccggcgc tttgacggc gaaatgtgc cccatttcg 
      cgtcggttt ttgcaacta taaattgtg aggtcggaa 
      aaagcctaa ttaaaaaat cgagtggga gtgtcataa 
      tttgacatt taaaaaaac taaaatttt ctttgttgt 
      cggcctgtc cgttgacct tggattcca acctatttg 
      tttacatgc tggatggat ggaagttct tcgattttc 
      tgaattatt gcagtattc ataatggtt gtggatgta 
      cctttgagg agttttcat cacaaaatg cagcctact 
      ggcatgagt cctcgtgaa ttatccgaa aacgatgac 
      tgggctaca tccctggtg ctagaccca catctcgga 
      ttcaccaca cataaaatg aaccttcgg tacaggccc 
      atcaaaacc agcacagct gaacttaaa agtattgtg 
      gaagagttt caaaaaact cagaattac gagaaagcc 
      tacaacagt attgccaag ggagaatgg atgcccaaa 
      atcaagtcc aaaatccag cagaacaaa ctcaaggag 
      catatttat cgctatcta agggtattc gacaaagag 
      tcgggtttc gtgatcgaa ccttgttat cgttactcg 
      ctcgagggt caaaagggc gctaaaatc tcagcgacg 
      aaaaaatgg tacaaaaac gacaaaatc gaatgtttg 
      gtcggttgc atcgccgaa ttgaccgaa gaagaggaa 
      aagcagctt cttcatccg ggaaaaaac gacttttcc 
      gtgatgtac agttgccga aagaattgt gcacaactc 
      tggttgggc cctgctgcg tatatcaat cacgattgc 
      agggcaaat tgtaaattc gtggctact ggtagagac 
      acggcgtgt gttaaagtt ttgcgcgac attgaagcg 
      ggtgaagag attacgtgt ttctacggt gaagatttc 
      ttcggtgat aaaaattgc tattgcgag tgcgaaacg 
      tgtgaaaga cgcggaatg ggcgctttt gcgaaagaa 
      agcgacaaa aaggaggag aacggttac agattgcgc 
      gaaaccgat aaccgaatt aaccggacc aagcagaaa 
      atcacgtgt aataataac aaagaggaa cagtttggt 
      ggtaatcgc ggcgaggag aaaatcgtg gcgccgttg 
      agtatgaaa gaattgaga cagaagggg ttgacgaag 
      tacgatgct gaacttttg atagcgcag gggtgcaag 
      ttttcggat attggggag tttccgagg aagaaccgg 
      ttaagtgtg tcgagtgcg ggtaatgac aataataac 
      aagaaatct gaagttaaa aaatctgtg ataaatgaa 
      gaaccgcaa gccttggtt agttcgagg acttcgaga 
      cttcaaaga agacaggcg agacaagca aatcaggag 
      cttatgcaa actgccact cataatgtg tcgagatcc 
      aatctttta gatgacttg cagagtaac gcgagtagt 
      tgtatgagc acttgtagc gatcaagat ccagtttgt 
      gatgccaca cagcagaat aagaagttt aatgacaaa 
      cacgagagc ggaaaaggg gtgccgcag attacagat 
      cgccccccg actccggtg caaacaaac gagaacggc 
      atcacattg cgtagccac aaatgcctc aatgtcgaa 
      aaatctgag agtgtaatg ccgaaattg gaacctcag 
      gtcgtctgt aaccaaaac ggtattaaa cacttatca 
      aaacgtggc acagctcgc gtgaaaacc cgtcattca 
      tcgcatctc accaagttg aataacaat atcactgcc 
      aataacgat aaagacgta tacgagttc gaggacgaa 
      gactcgaag acggactcc ttatgtctg cgaaggaac 
      agaacgaaa gaaacgaac ggtgataat gtgataaaa 
      cccgaaatt gataacgag gattcgagg gaaaagtct 
      gacgataat cagaacaat tggcacgag gttaaaacc 
      gaaccggtt ttcgtgccg gagacgacc caagtggag 
      aaaacaccg gaaaagtgc ggacggttg aaattgacg 
      ttgaggatg aaacggagc ccggtcttg gatgaagtt 
      atcgagtcc gggaatagt ctgagtgaa gacagtttc 
      gaaccggag tatgaagtg ttgagagtg gagggcgta 
      gagtataca cctttttca caccggaaa aagagacat 
      aaatcgaag gacaggaaa agggatagg aaattaaaa 
      caaagtgaa gaggtgacc catttacca atgaagaga 
      ttacggtta atatttggg aatgagagt cacacgatc 
      gatatcccg tccactagt tcaaattgt catacatga 
      ctttttccg ttctgatgt aacacgatt cctttgtta 
      gttttattt ttgtaattg tacagcagg acctttttt 
      atttattta ttttactaa ctttctaac gtttaagtg 
      aattttgtc ttttggatg ttagggttg tggcgcact 
      cgatcttcg ctaagattt acaagtctt ttatttatt 
      tgggtgttt taggcgtga attgatctc atcaggtgt 
      ttataaaac aatatgaca cacgagaag tgtgataca 
      acaattagg tttatcgtg aaactttgc ataaaaatc 
      gttacttgt gtgcacgaa tgattgtgt gaacgtaat 
      taaaagact tgtatattt atgaaaaat gctagattg 
      agagtcgtc attcataaa atagaccgc tgtgattca 
      gaatgttag cgttagtga gaccaaact actttggtc 
      ggaagctta tttttaata aatcgtgta aaatgtaat 
      attttattt gtggtattc tttcagaac cgaccggat 
      gcgttgtat tagcggtta ggggattct aacaaataa 
      aatttcagg acttgttgc cttcgttgt gcagtaata 
      tacaaaaaa ccattccat ttaaacaga tcattattt 
      attttattt atgtacaag gacacagat cttggtata 
      tttttgttg aatgggtgc acacacctt agattagat 
      tttttccat agttttatt ttcacaagc atttatcga 
      aatggtggg ttgagtgag cgccgtacg ctgttctat 
      tacgttttt agataaatt cttgttgaa atttactgt 
      tgaaatttt atgatgtct gtggagaag tgtatgcat 
      ctgtgaata atgtaattc atcgagatt ggtgcatat 
      tgcacggta tttgtaaat atatttgta atatgtacg 
      ataagaatt atgcattac ttaaattta cctcatgtt 
      tatatgaaa acacatcat cgatcattt cttacattg 
      tttcggcgt tgatcccct taatgagtt cctaatgct 
      cccacaatt agttcatct ttgcgtgaa tgcagtgca 
      tgtgtgcgt ggatgtgtt atgtattaa tgtaagaaa 
      tgataacaa tgtactaaa ctgaataaa gttttataa 
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY