Status
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Gene information
General information
TC008321 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8453672..8463225
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008321
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04464

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008321 RA
      MDDSSPDLS LKLRRGSSD SRDSFYMDF DKGIDSDIE 
      EMATTSAAD LGDTTADTT ATPTALGYG KTEGESWPT 
      IPPQNMSGG SRSSALGGG DPLLPSPAS PSAVIVSPE 
      TLSHHSSPS KSTKQTAHV QIAPPPKTL SPGPLAVAP 
      LGYHQQFPV MRRLSRAEK QIEWRQLGA DALATTLSA 
      LYGKLLVVM GTAFPMAEV ISTYIPPSF YEAYYLYLY 
      IGSMFFLLY MYTVLLRDK RKRKRVAEA NNKSSKGKE 
      ESISDEESD NDTGETSAD TASSQIYNR PRRSSLPTV 
      TKQNHYGSF YLRMGAVAF GIGSMIYSG LEFGQYFEL 
      ERDTKCHNV LLALTPATR MAFIFIQMY FIFLNNEQM 
      RVYRHKVAA RFGLMHMIG TNLSVWLNV LVQETKHEI 
      LTFYNPENN SLRISHRIP YKNNILGLP AVPMDHPPE 
      AHGHHIVAR GLKGPHHLY ECRRTNIIG SLVQDASPF 
      LFPCTIEYS LICAAILYV MWKNISKLP LTGARAKSD 
      LSAYKRSPH HYSVDCARA HKGLFVGIL LLVLTIISL 
      ILFFVLISR SEFVGLAVI EVNICELTL YGLTTLAAI 
      IGMWQVRQL KYDGSKNLQ LDNILLIGA QTGMFIYST 
      FTIIGGQFT IQRNTVLVL VTALASLLQ TTCQTIFIL 
      DASRRSCVT PEQIRRKPG RQIVTFLLV TNLAMWAIN 
      TLEKSRAES HPIQLHFYG LWAWTIITH VSMPLAIFY 
      RFHSTVCLC EIWKRAYKI KPTYM
    CDS sequence
    • >TC008321 RA
      atggatgat agctcgcct gatttgagt ctcaagctg 
      aggcgagga tctagcgat tcgagagat tcgttttac 
      atggatttt gataaagga atcgattcg gatatcgaa 
      gaaatggca actacttcc gctgctgac ctcggggac 
      acgactgcg gacaccaca gcaaccccc actgcttta 
      gggtacggg aaaactgaa ggcgaaagt tggccgacg 
      ataccgccc cagaacatg agcgggggc agtcggagc 
      agtgcactt gggggtggc gaccctttg ctaccatct 
      ccagcatcc ccttctgct gtcatagtc tcaccagaa 
      acgttgagt catcattct tctccgtca aaatccacc 
      aaacaaact gcacacgtc caaatcgct cctcccccg 
      aaaactctg tcacctgga ccactcgcc gtggcaccc 
      ctaggctac catcagcag ttcccagtc atgaggagg 
      ctttcacgt gcggaaaaa cagattgaa tggagacaa 
      ctcggggcc gacgctctg gcgacgact ttgtcggct 
      ctgtacggg aaattattg gtggtgatg ggaacagca 
      tttcctatg gcggaagtt atttcaacg tatattcct 
      ccttcgttc tacgaagcg tactatctt tatctctac 
      attggaagc atgtttttc ctcctctac atgtacacc 
      gtccttctg cgagataaa agaaaaagg aaacgagtt 
      gcagaagct aataacaaa agctcgaaa ggtaaggaa 
      gagagtatt tcagatgaa gagtctgat aatgacacg 
      ggggaaacg agtgctgat acggccagt tcacaaatc 
      tataatcgc ccgaggagg agttctttg cccacagtt 
      accaaacaa aaccactac ggaagcttt tatctccgt 
      atgggagcc gttgctttc ggaatcggc agcatgatc 
      tactcagga ctcgaattt ggacaatac ttcgaactc 
      gaacgggac accaaatgc cataacgtc ctcctcgcc 
      ctaactccg gccactcgc atggctttt attttcatc 
      caaatgtat ttcatcttt ctgaataac gagcaaatg 
      cgagtttac aggcacaaa gtcgctgca aggttcggc 
      ttaatgcac atgatcggg actaatctc tccgtctgg 
      ttgaacgtc ctagtccaa gagaccaaa cacgagatt 
      ctgaccttc tacaacccg gaaaataac tcactccgg 
      atctctcac cggatccct tacaaaaac aatattttg 
      gggcttccg gccgtcccg atggaccat ccacccgaa 
      gcgcacggc caccatatt gtggcgcgg gggctcaaa 
      gggccccac cacctctac gagtgccgg cgcactaat 
      attataggg tctctagtc caagatgct tcacctttc 
      ctttttcct tgcacgatc gaatacagt ctcatttgc 
      gcggctatt ttgtatgtc atgtggaaa aacatatcc 
      aagctgccc ttaactggg gccagggcc aagagcgac 
      ctttcggcg tacaaacgg agccctcat cactattca 
      gtcgactgc gcaagggcc cacaaaggc ctcttcgtg 
      gggattctc ctcctggtc ctcacaatc atctccctg 
      attttgttc tttgtcttg atctcgcgg tcggaattt 
      gtcggtttg gcggtcatt gaagtcaac atttgcgag 
      ttgactctt tacggcttg accactctt gcggccata 
      atcggcatg tggcaagtc cgccaactc aaatacgac 
      ggcagtaag aatttacaa ttggataac attctccta 
      atcggcgcc caaactggg atgtttatt tacagcacg 
      tttacaata atcggtggt cagttcacg attcagcgc 
      aacactgtt ttggtccta gtgacggct ttagcttcc 
      cttttgcaa accacttgt cagacgatt tttattttg 
      gacgcgtcc aggcgtagt tgtgtcact ccggagcag 
      atccggcgg aaacccggg cgccaaatc gtcactttc 
      ctgctggtg acgaatttg gctatgtgg gctattaac 
      acactggag aagtcgcgg gcagagtcg caccccatc 
      cagttgcat ttttacggg ttgtgggcc tggaccatc 
      atcacccat gtctcaatg cccctggcc attttctac 
      cggtttcat tcgaccgtt tgcttgtgt gagatttgg 
      aagcgcgct tacaagatc aaaccgact tatatgtag
    mRNA sequence
    • >TC008321 RA
      ctccaacat aaaagagac ccgagcatc agttgagtg 
      tggacggtg ttggaagcg ttaaaatgt aaaattacg 
      cgattctga gtaaaacga gtgctcaac acgttcaga 
      aattgtgtg atgtagccg ctagcgttg cagtaacaa 
      cccttgcta caaaaattc aacaaaata attcgtagc 
      tcgaaaaaa ttccgtgat gtgattatt aatcagtag 
      cgggttcct gctgaagta gtaccattt ggtactacg 
      tcacgtggc caaagacaa tgctgaaat aatttagaa 
      tgtcttaag tgtgtgcgg tgaaaaaat cgattcaca 
      atggatgat agctcgcct gatttgagt ctcaagctg 
      aggcgagga tctagcgat tcgagagat tcgttttac 
      atggatttt gataaagga atcgattcg gatatcgaa 
      gaaatggca actacttcc gctgctgac ctcggggac 
      acgactgcg gacaccaca gcaaccccc actgcttta 
      gggtacggg aaaactgaa ggcgaaagt tggccgacg 
      ataccgccc cagaacatg agcgggggc agtcggagc 
      agtgcactt gggggtggc gaccctttg ctaccatct 
      ccagcatcc ccttctgct gtcatagtc tcaccagaa 
      acgttgagt catcattct tctccgtca aaatccacc 
      aaacaaact gcacacgtc caaatcgct cctcccccg 
      aaaactctg tcacctgga ccactcgcc gtggcaccc 
      ctaggctac catcagcag ttcccagtc atgaggagg 
      ctttcacgt gcggaaaaa cagattgaa tggagacaa 
      ctcggggcc gacgctctg gcgacgact ttgtcggct 
      ctgtacggg aaattattg gtggtgatg ggaacagca 
      tttcctatg gcggaagtt atttcaacg tatattcct 
      ccttcgttc tacgaagcg tactatctt tatctctac 
      attggaagc atgtttttc ctcctctac atgtacacc 
      gtccttctg cgagataaa agaaaaagg aaacgagtt 
      gcagaagct aataacaaa agctcgaaa ggtaaggaa 
      gagagtatt tcagatgaa gagtctgat aatgacacg 
      ggggaaacg agtgctgat acggccagt tcacaaatc 
      tataatcgc ccgaggagg agttctttg cccacagtt 
      accaaacaa aaccactac ggaagcttt tatctccgt 
      atgggagcc gttgctttc ggaatcggc agcatgatc 
      tactcagga ctcgaattt ggacaatac ttcgaactc 
      gaacgggac accaaatgc cataacgtc ctcctcgcc 
      ctaactccg gccactcgc atggctttt attttcatc 
      caaatgtat ttcatcttt ctgaataac gagcaaatg 
      cgagtttac aggcacaaa gtcgctgca aggttcggc 
      ttaatgcac atgatcggg actaatctc tccgtctgg 
      ttgaacgtc ctagtccaa gagaccaaa cacgagatt 
      ctgaccttc tacaacccg gaaaataac tcactccgg 
      atctctcac cggatccct tacaaaaac aatattttg 
      gggcttccg gccgtcccg atggaccat ccacccgaa 
      gcgcacggc caccatatt gtggcgcgg gggctcaaa 
      gggccccac cacctctac gagtgccgg cgcactaat 
      attataggg tctctagtc caagatgct tcacctttc 
      ctttttcct tgcacgatc gaatacagt ctcatttgc 
      gcggctatt ttgtatgtc atgtggaaa aacatatcc 
      aagctgccc ttaactggg gccagggcc aagagcgac 
      ctttcggcg tacaaacgg agccctcat cactattca 
      gtcgactgc gcaagggcc cacaaaggc ctcttcgtg 
      gggattctc ctcctggtc ctcacaatc atctccctg 
      attttgttc tttgtcttg atctcgcgg tcggaattt 
      gtcggtttg gcggtcatt gaagtcaac atttgcgag 
      ttgactctt tacggcttg accactctt gcggccata 
      atcggcatg tggcaagtc cgccaactc aaatacgac 
      ggcagtaag aatttacaa ttggataac attctccta 
      atcggcgcc caaactggg atgtttatt tacagcacg 
      tttacaata atcggtggt cagttcacg attcagcgc 
      aacactgtt ttggtccta gtgacggct ttagcttcc 
      cttttgcaa accacttgt cagacgatt tttattttg 
      gacgcgtcc aggcgtagt tgtgtcact ccggagcag 
      atccggcgg aaacccggg cgccaaatc gtcactttc 
      ctgctggtg acgaatttg gctatgtgg gctattaac 
      acactggag aagtcgcgg gcagagtcg caccccatc 
      cagttgcat ttttacggg ttgtgggcc tggaccatc 
      atcacccat gtctcaatg cccctggcc attttctac 
      cggtttcat tcgaccgtt tgcttgtgt gagatttgg 
      aagcgcgct tacaagatc aaaccgact tatatgtag 
      cggcttgtg gtgttctag tcttggctt tttattgtt 
      taaaaggtt gaactataa ccgccaaga gagtgaatt 
      tttaagcag tatagttca gccttaaga ctgaagtgg 
      caaggatgg tcgatgttg tgtgtgaga ttaagcttc 
      gtttattga tcatgaaag tctagtttc aagtaacgt 
      aagtcgtct ttaattaaa tcttgtatt ttatgacag 
      tgtattacg agtgtgtgt tacataact tattggtag 
      ttgcaagac ttcacggaa ccggatatg caaggattt 
      ttgcgattc cctaagcga tcattctaa cgtagagta 
      gttaacata tcctaaata cgtagcatg ggttacaag 
      actgactcc ggaatgctc aaaattagt tcgttcagt 
      tcatatttt tggagagat tttaatacg cctaattgc 
      ttaagtgaa gagtttcat ccgccacaa cacaaatca 
      aattagagt tggacacaa aggccctta attgcgtag 
      taaccgaag tttgatcaa tgcagggtt gacaatgtt 
      caaacaata atcttttcg aacgtcaga tttacacca 
      aaaactatt ttcgtatta gtgtttgta ggagtagct 
      aacccacct tcatatcta accttttta tacttctca 
      cattttaca catttttat agcaattta caaagccgt 
      cttccttta tgaaatagc tgttaaagt acaaagttc 
      aattttgtc ctgcctatt aataaataa gcaccttag 
      taccactta catacttgg ctcaatagc tttttaatt 
      acaatggat gtttagatt tctcgcaac aacgagatt 
      tttgttaaa acttttgtt gatgatctc gttgactaa 
      taatccttt gttatgtac aaatttttg ggaacaata 
      ttttgtagt ttatgagaa tagatgcgt cgtaacgat 
      tttgataag gtgtaagtt ccacgtgta aaatgggtc 
      gacgttaaa atgtatccg gacaaggac ccactttat 
      acaaatggc acttactta ttagtctag ttttaaatg 
      ttacatttg tgtatttat tttagaaaa attaatggt 
      gccaatcag aaatgcttt cagccatgt tttagttat 
      gttttgtgc catattagt ttagagatt tgtaaatta 
      tttgttatc catttacaa ttttattgt atatagcta 
      tcatttgac ttatttaaa tgttaattt tacttgcaa 
      gttatttac taaatgtat cgtaataaa atatcacac 
      tttac
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