Status
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Gene information
General information
TC008304 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8160414..8174909
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008304
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04461

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008304 RA
      MLCGEFHKA PLGFYVNFA SLSDSRSPY SSLLTLVQS 
      MAALTAHAA HEEEDIFLE NGLLSYENP NYHLDPTRI 
      DDALNSNTN LYEDIYQEL DDICNIGHS IKEPGRKAY 
      AALDIGTMG VDITSGPLT NINDNKRSN ERNGNAYVM 
      DNSEKRATR ENISEKTAK NNKDNQEKG VPHIPGQLN 
      NDLYAVPVK KKNQKCAEE IEIYSPDKE SADDTDLPP 
      GWEKHEDND GPYYWHIKS GTIQREVPV LPESEKPKK 
      LDSKHNINK ENDVLMMTF ENSIGSVTR SSTSSALDL 
      ESEDRKRKN DLTFKRRSY PSKPEQESR ERPIRFAVR 
      SLGWVEIAE EDLTPERSS KAVNKCIVD LSVGKNDLL 
      DVVGRWGDG KDLFMDLDE GALKLIDPE NLTVLNTQP 
      IHTIRVWGV GRDNGRDFA YVARDRSTR KHMCHVFRC 
      DMPARTIAN TLRDICKKI MIERSLQQN LAKPIDING 
      RTLATRPTN LPTEHRRLT RNGQSLVTQ SFPTPMEEP 
      KKVLRAQYL GTTQVSQAT GMEVLNEAI DRLVATVPP 
      EQWQCVNVA VAPSMISIQ CPSDDRLIA ECRVRYLSF 
      LGIGKIIKH CAFVMHTAQ DTFVAHVFY CEPSSGALC 
      KTIEAACKL RYQKCLDAH PQGLERNNS NLHTPGKSI 
      GAALKSIVG SLTGRKSRS SDS
    CDS sequence
    • >TC008304 RA
      atgttgtgt ggcgagttc cacaaagcg cctctcggt 
      ttctacgta aattttgcg agcctttcg gattcccgt 
      agtccgtat tcgtctttg cttactcta gtccagagc 
      atggcagct ttaacggct catgcagcg cacgaagaa 
      gaggatata tttctcgaa aacggtctt ttaagctac 
      gaaaatcca aactatcat ttggacccg acgcgtatc 
      gacgatgct ctaaattca aacacgaat ctctacgag 
      gatatttat caggagttg gatgatatt tgcaatatc 
      gggcacagt atcaaggag cctgggcgg aaggcgtac 
      gctgccttg gatatcggc accatgggg gtggacatc 
      acgagcggg cccttgacg aacatcaat gacaataaa 
      aggagtaat gaacgcaat ggaaatgca tacgtcatg 
      gataacagc gaaaaaaga gcaacgcga gaaaacatt 
      agcgagaag acggcgaag aacaacaag gacaatcag 
      gagaagggc gtgccacac atacccggc cagcttaat 
      aatgattta tatgccgtt ccggtgaag aaaaagaat 
      caaaaatgt gcggaagaa attgagata tactcgccc 
      gataaagag agcgccgac gatactgat ttacctccc 
      ggctgggag aagcacgaa gacaatgat ggcccctac 
      tattggcac ataaagagc ggaacgatc cagcgcgag 
      gtcccagtg ctacccgag agcgaaaaa ccgaaaaaa 
      ctcgactcg aaacacaac attaataaa gaaaacgat 
      gttttaatg atgactttt gagaacagc atcggctcc 
      gtcaccaga agtagcacc agctcggcc ctggacctc 
      gagtcggag gaccgcaag cggaaaaat gatctgact 
      tttaaacgg cgaagttac ccttcgaag cctgagcaa 
      gagtcacgc gagcgacca atccgattc gcggttcgg 
      tctttgggc tgggttgaa atcgccgaa gaggatttg 
      actccggag aggagtagt aaagctgta aataaatgt 
      attgttgat ttatcggtt ggaaagaat gaccttttg 
      gatgttgtg ggccgatgg ggagatggt aaggatttg 
      ttcatggat ttggacgag ggtgcgtta aaattaatc 
      gatccggag aatctgacc gttttaaat actcaacct 
      attcacact ataagggtt tggggagtg ggacgcgat 
      aatggaaga gattttgct tacgtagct agggaccga 
      agtacccgg aaacacatg tgtcacgtg tttcgttgc 
      gacatgcct gcccgcact attgccaat actttaaga 
      gacatttgt aaaaaaata atgatagaa cgaagtttg 
      caacagaat ttagctaaa ccaatcgat atcaacggt 
      cggacgtta gctacgcga cctacaaac ctaccaact 
      gaacatcga cgattaact cgtaacggc cagtcttta 
      gttacccag tcgtttccc actcctatg gaagaacca 
      aagaaagta cttagggct caatatttg ggcaccact 
      caagtctca caagccact ggaatggaa gttttgaac 
      gaggctatt gacagactt gtggcgact gtccctcca 
      gagcagtgg cagtgtgtt aacgtagct gtggctccg 
      tctatgata tcaatccaa tgtccaagt gacgatagg 
      ctgatagcg gaatgccgc gtccgctac ttgtccttc 
      ttaggcata ggaaaaatt atcaaacac tgtgctttc 
      gtaatgcat acggcacag gacactttt gttgcacat 
      gtgttttac tgcgaaccg tcttctggt gccctttgt 
      aagacaatt gaggcggct tgcaagttg agataccag 
      aagtgtctc gacgcgcac cctcaaggt ctcgaacga 
      aacaattca aacctccac actcctgga aaaagtatt 
      ggagcggcg ttgaaatca atcgttggg tctttaacc 
      ggacggaaa agtaggagt agtgattct tga
    mRNA sequence
    • >TC008304 RA
      cagttaagc agaaaatta aagctacat ttgcatata 
      acgggagct taatttgct actagacaa gaaataaat 
      aataatcaa ataaaaaaa ctgtaaaat tattaaaat 
      cggtggtag aggtgtggt ttggaagag gcttggcct 
      cccactttt ttgacaatc gaaccattt ctaccaact 
      ctcgattca ctcagtgtc tacttcggt tgtagagag 
      gttaataac accaataaa ttattttat tgagtgagt 
      actgtgctc aaattttca tattcgtat tgattttcg 
      tagtttcta gttagaaaa agtagtgga tattgctac 
      aactgcatg ggaggtatt agggctaag cgctgctct 
      ccattctgc agggggagg tagaaagga atggtagtt 
      ctgctcggg gatgttgtg tggcgagtt ccacaaagc 
      gcctctcgg tttctacgt aaattttgc gagcctttc 
      ggattcccg tagtccgta ttcgtcttt gcttactct 
      agtccagag catggcagc tttaacggc tcatgcagc 
      gcacgaaga agaggatat atttctcga aaacggtct 
      tttaagcta cgaaaatcc aaactatca tttggaccc 
      gacgcgtat cgacgatgc tctaaattc aaacacgaa 
      tctctacga ggatattta tcaggagtt ggatgatat 
      ttgcaatat cgggcacag tatcaagga gcctgggcg 
      gaaggcgta cgctgcctt ggatatcgg caccatggg 
      ggtggacat cacgagcgg gcccttgac gaacatcaa 
      tgacaataa aaggagtaa tgaacgcaa tggaaatgc 
      atacgtcat ggataacag cgaaaaaag agcaacgcg 
      agaaaacat tagcgagaa gacggcgaa gaacaacaa 
      ggacaatca ggagaaggg cgtgccaca catacccgg 
      ccagcttaa taatgattt atatgccgt tccggtgaa 
      gaaaaagaa tcaaaaatg tgcggaaga aattgagat 
      atactcgcc cgataaaga gagcgccga cgatactga 
      tttacctcc cggctggga gaagcacga agacaatga 
      tggccccta ctattggca cataaagag cggaacgat 
      ccagcgcga ggtcccagt gctacccga gagcgaaaa 
      accgaaaaa actcgactc gaaacacaa cattaataa 
      agaaaacga tgttttaat gatgacttt tgagaacag 
      catcggctc cgtcaccag aagtagcac cagctcggc 
      cctggacct cgagtcgga ggaccgcaa gcggaaaaa 
      tgatctgac ttttaaacg gcgaagtta cccttcgaa 
      gcctgagca agagtcacg cgagcgacc aatccgatt 
      cgcggttcg gtctttggg ctgggttga aatcgccga 
      agaggattt gactccgga gaggagtag taaagctgt 
      aaataaatg tattgttga tttatcggt tggaaagaa 
      tgacctttt ggatgttgt gggccgatg gggagatgg 
      taaggattt gttcatgga tttggacga gggtgcgtt 
      aaaattaat cgatccgga gaatctgac cgttttaaa 
      tactcaacc tattcacac tataagggt ttggggagt 
      gggacgcga taatggaag agattttgc ttacgtagc 
      tagggaccg aagtacccg gaaacacat gtgtcacgt 
      gtttcgttg cgacatgcc tgcccgcac tattgccaa 
      tactttaag agacatttg taaaaaaat aatgataga 
      acgaagttt gcaacagaa tttagctaa accaatcga 
      tatcaacgg tcggacgtt agctacgcg acctacaaa 
      cctaccaac tgaacatcg acgattaac tcgtaacgg 
      ccagtcttt agttaccca gtcgtttcc cactcctat 
      ggaagaacc aaagaaagt acttagggc tcaatattt 
      gggcaccac tcaagtctc acaagccac tggaatgga 
      agttttgaa cgaggctat tgacagact tgtggcgac 
      tgtccctcc agagcagtg gcagtgtgt taacgtagc 
      tgtggctcc gtctatgat atcaatcca atgtccaag 
      tgacgatag gctgatagc ggaatgccg cgtccgcta 
      cttgtcctt cttaggcat aggaaaaat tatcaaaca 
      ctgtgcttt cgtaatgca tacggcaca ggacacttt 
      tgttgcaca tgtgtttta ctgcgaacc gtcttctgg 
      tgccctttg taagacaat tgaggcggc ttgcaagtt 
      gagatacca gaagtgtct cgacgcgca ccctcaagg 
      tctcgaacg aaacaattc aaacctcca cactcctgg 
      aaaaagtat tggagcggc gttgaaatc aatcgttgg 
      gtctttaac cggacggaa aagtaggag tagtgattc 
      ttgagatac ggtaaaatc tctgcaggt acttgttgt 
      aggttgtaa gatggctta gaagtaaag tgatttttt 
      aaggaattg tgtctcttg cctgtggag cgcgaggta 
      atcaaacac agacatctg caatctcct ctaacccag 
      aaatacatt acttcaact tcttcggcg gatcttcgt 
      tctctgttg tctcctgtg ttggatgat ttgaagcac 
      attagctgg gattcggct cgttaagct ggttaacaa 
      taaaactgt acaaaattt gtgttaaga ctcgatagt 
      atcacgtgg tgtttgtca caatgcact aactaacaa 
      gacgcttct aaatatgat agattagga cacaagcac 
      ttcttgtga gcctcagtt aggtagtct gcaacgaga 
      tggtctctc atatttaga gttagttac tctgcctct 
      atatgtttt agctgtcgt aaggtgatc aacggtgtt 
      actttaacg cttcatcaa ggtgtaaat atgtagtca 
      aaagtgtct tggtgtttt gggggttgg tcactttat 
      actggtact tgtagcttg cttcaagcc tcggcaaca 
      aatcacaac aacacccag ctaaagaca atattacat 
      taaggtttt acaaaattg taaaaaatc aaaagggcg 
      ggaggattg tctttacac gtgcgatct tagttaaga 
      aatgcaatg tttatgtaa atactaata agcctttca 
      tgttcttag gtcagtacg ttttatatt ttttccggc 
      tttggtgta cgaattgag agacgtgca atcttgcca 
      ttgctattc tttcctcag ttgtcgttt atctattgt 
      atgccttta cgcaaagat atttgttta ctacaatat 
      ttttataaa aaatgaatg tatgcaatg atcgtagtc 
      tgcaaccga ttgtttcac tcaccgttt cgttggtcg 
      cttgtaatg taaatatgc cagacttgt agtttcaag 
      aacagttaa tgtaaaatt tccttatta acaacaatt 
      gtccaataa attgcatac tcttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY