Status
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Gene information
General information
TC008298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8107648..8112068
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008298
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04460

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    pygopodium not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008298 RA
      MGGFTQRPW TPTKRRGPI AAEYNSPGP ACVSLPSYI 
      GKKTSESKS GRAPAFSFG ARHNGKIDS IGPGPGQYN 
      VTGLSAKGK DTPPAVSLH GRPKEAKPE NFPAPGDYD 
      PDKAEKVIH DNAPRYTFG LKTNVEKPL DTPAPNVYN 
      VPESEKKLH EHSPKYSFG LKTPIEKPS DTPAPNVYN 
      VPTADKVLH ENSPKYSFG CKVQLEKPS ETPAPNVYN 
      VPNADKVLH ENSPRYSFG NKVQLEKPS DTPAPNVYN 
      VPTSEKILH DNSPKYSFG NKVQLEKPL DTPAPNVYN 
      IPPVVGDKK SAPAYTISG RNKDPVDER VKNPGPGQY 
      NNVNPESYK THSPAYTIS GRTNIPVDS TTPGPGVYS 
      PEKVDLDYH PAHSFGIRH SQYAEHY
    CDS sequence
    • >TC008298 RA
      atgggagga tttactcaa cgtccatgg actccaaca 
      aaacgacgt ggccccata gccgcagaa tataacagt 
      ccaggccca gcatgtgtg tctcttcca tcttacata 
      ggtaaaaaa acatcggaa tcaaaaagt gggcgagca 
      ccggctttc agtttcggt gctagacat aatggcaaa 
      attgacagc atcggacct ggtccgggt caatataac 
      gtaactggt cttagtgca aaaggaaaa gatacgccg 
      ccagctgtt agtttgcac ggtcgtccc aaagaagcc 
      aagcctgaa aatttcccc gcacctggg gattatgat 
      cctgataaa gccgagaaa gttattcac gacaacgca 
      ccgcgatat acttttggt ttaaagaca aatgtcgaa 
      aaaccgttg gacacacca gcacccaat gtttacaat 
      gtaccagaa tccgaaaaa aaattgcat gaacattcc 
      ccaaaatac agtttcgga cttaaaact ccaatcgaa 
      aaaccatct gacacaccg gcgcccaat gtttacaac 
      gtcccaacg gccgacaag gttttacat gaaaattct 
      cccaaatac agttttgga tgcaaggtt cagttggaa 
      aagccttcg gaaacaccc gctcccaat gtatacaat 
      gttccaaat gccgataaa gtgttacac gagaattcc 
      cctagatac agttttggc aacaaagtt caattggaa 
      aagccatcg gatacccct gctccaaat gtttacaac 
      gtgccgact tccgaaaag atcctacac gataattca 
      cctaaatac agttttgga aataaggtt caactggaa 
      aaacctttg gacactcct gcacccaat gtgtacaac 
      attccgcca gttgttggt gacaaaaaa tccgcacct 
      gcttacacc atatctgga agaaacaag gacccggtt 
      gacgaacgc gtaaaaaat ccgggtcca ggccaatat 
      aacaacgtc aatccggaa agctacaaa actcattcg 
      cctgcatac accataagt gggcgaaca aatattcca 
      gttgattcc acaactcca gggcccgga gtctattct 
      ccggaaaag gtggatctc gattatcat cctgcacac 
      agttttggc atcaggcac tcccaatac gcagagcat 
      tattaa
    mRNA sequence
    • >TC008298 RA
      tggtggggg cctttacat agccagcag ttttgaaaa 
      ttttgaatt aaaacgtga ttaaaacta gtacaggat 
      ctgtgctgt tcggggttg agtgttcgc cttgactta 
      tttattatt cggataaaa tttctcaca acacaatta 
      aaaaaatgg gaggattta ctcaacgtc catggactc 
      caacaaaac gacgtggcc ccatagccg cagaatata 
      acagtccag gcccagcat gtgtgtctc ttccatctt 
      acataggta aaaaaacat cggaatcaa aaagtgggc 
      gagcaccgg ctttcagtt tcggtgcta gacataatg 
      gcaaaattg acagcatcg gacctggtc cgggtcaat 
      ataacgtaa ctggtctta gtgcaaaag gaaaagata 
      cgccgccag ctgttagtt tgcacggtc gtcccaaag 
      aagccaagc ctgaaaatt tccccgcac ctggggatt 
      atgatcctg ataaagccg agaaagtta ttcacgaca 
      acgcaccgc gatatactt ttggtttaa agacaaatg 
      tcgaaaaac cgttggaca caccagcac ccaatgttt 
      acaatgtac cagaatccg aaaaaaaat tgcatgaac 
      attccccaa aatacagtt tcggactta aaactccaa 
      tcgaaaaac catctgaca caccggcgc ccaatgttt 
      acaacgtcc caacggccg acaaggttt tacatgaaa 
      attctccca aatacagtt ttggatgca aggttcagt 
      tggaaaagc cttcggaaa cacccgctc ccaatgtat 
      acaatgttc caaatgccg ataaagtgt tacacgaga 
      attccccta gatacagtt ttggcaaca aagttcaat 
      tggaaaagc catcggata cccctgctc caaatgttt 
      acaacgtgc cgacttccg aaaagatcc tacacgata 
      attcaccta aatacagtt ttggaaata aggttcaac 
      tggaaaaac ctttggaca ctcctgcac ccaatgtgt 
      acaacattc cgccagttg ttggtgaca aaaaatccg 
      cacctgctt acaccatat ctggaagaa acaaggacc 
      cggttgacg aacgcgtaa aaaatccgg gtccaggcc 
      aatataaca acgtcaatc cggaaagct acaaaactc 
      attcgcctg catacacca taagtgggc gaacaaata 
      ttccagttg attccacaa ctccagggc ccggagtct 
      attctccgg aaaaggtgg atctcgatt atcatcctg 
      cacacagtt ttggcatca ggcactccc aatacgcag 
      agcattatt aaaaaaata aagttttca tcaaccgca 
      gtatacgtt agttaacat cgttcacaa taattattg 
      tgaagcaga aagcgaatc tgctttttt aattaaaag 
      ctctctagg tacatcgta gttcctaaa ctgtgcaca 
      attattgga tttgccaag cttatgaaa acgcttata 
      atttaaccg cactataat tgcgaaaag ttttattca 
      tgttggtag cgttaaccc actgttatg accggtttt 
      agtttaatt agacaaagc gactaattt ttgttttat 
      tgccatttt tttcaatgc tctctagcg cgtaatcta 
      cacaagaga acaaccagc gattatata catttataa 
      ttaaaataa ccatgtatt tgtcttttt tattaaata 
      aagttttat atattga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY