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Gene information
General information
TC008298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8107648..8112068
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008298
    iBeetle screen iB_04460

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    pygopodium not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008298 RA
      MGGFTQRPW TPTKRRGPI AAEYNSPGP ACVSLPSYI 
GKKTSESKS GRAPAFSFG ARHNGKIDS IGPGPGQYN 
VTGLSAKGK DTPPAVSLH GRPKEAKPE NFPAPGDYD 
PDKAEKVIH DNAPRYTFG LKTNVEKPL DTPAPNVYN 
VPESEKKLH EHSPKYSFG LKTPIEKPS DTPAPNVYN 
VPTADKVLH ENSPKYSFG CKVQLEKPS ETPAPNVYN 
VPNADKVLH ENSPRYSFG NKVQLEKPS DTPAPNVYN 
VPTSEKILH DNSPKYSFG NKVQLEKPL DTPAPNVYN 
IPPVVGDKK SAPAYTISG RNKDPVDER VKNPGPGQY 
NNVNPESYK THSPAYTIS GRTNIPVDS TTPGPGVYS 
PEKVDLDYH PAHSFGIRH SQYAEHY
    CDS sequence
    • >TC008298 RA
      atgggagga tttactcaa cgtccatgg actccaaca 
aaacgacgt ggccccata gccgcagaa tataacagt 
ccaggccca gcatgtgtg tctcttcca tcttacata 
ggtaaaaaa acatcggaa tcaaaaagt gggcgagca 
ccggctttc agtttcggt gctagacat aatggcaaa 
attgacagc atcggacct ggtccgggt caatataac 
gtaactggt cttagtgca aaaggaaaa gatacgccg 
ccagctgtt agtttgcac ggtcgtccc aaagaagcc 
aagcctgaa aatttcccc gcacctggg gattatgat 
cctgataaa gccgagaaa gttattcac gacaacgca 
ccgcgatat acttttggt ttaaagaca aatgtcgaa 
aaaccgttg gacacacca gcacccaat gtttacaat 
gtaccagaa tccgaaaaa aaattgcat gaacattcc 
ccaaaatac agtttcgga cttaaaact ccaatcgaa 
aaaccatct gacacaccg gcgcccaat gtttacaac 
gtcccaacg gccgacaag gttttacat gaaaattct 
cccaaatac agttttgga tgcaaggtt cagttggaa 
aagccttcg gaaacaccc gctcccaat gtatacaat 
gttccaaat gccgataaa gtgttacac gagaattcc 
cctagatac agttttggc aacaaagtt caattggaa 
aagccatcg gatacccct gctccaaat gtttacaac 
gtgccgact tccgaaaag atcctacac gataattca 
cctaaatac agttttgga aataaggtt caactggaa 
aaacctttg gacactcct gcacccaat gtgtacaac 
attccgcca gttgttggt gacaaaaaa tccgcacct 
gcttacacc atatctgga agaaacaag gacccggtt 
gacgaacgc gtaaaaaat ccgggtcca ggccaatat 
aacaacgtc aatccggaa agctacaaa actcattcg 
cctgcatac accataagt gggcgaaca aatattcca 
gttgattcc acaactcca gggcccgga gtctattct 
ccggaaaag gtggatctc gattatcat cctgcacac 
agttttggc atcaggcac tcccaatac gcagagcat 
tattaa
    mRNA sequence
    • >TC008298 RA
      tggtggggg cctttacat agccagcag ttttgaaaa 
ttttgaatt aaaacgtga ttaaaacta gtacaggat 
ctgtgctgt tcggggttg agtgttcgc cttgactta 
tttattatt cggataaaa tttctcaca acacaatta 
aaaaaatgg gaggattta ctcaacgtc catggactc 
caacaaaac gacgtggcc ccatagccg cagaatata 
acagtccag gcccagcat gtgtgtctc ttccatctt 
acataggta aaaaaacat cggaatcaa aaagtgggc 
gagcaccgg ctttcagtt tcggtgcta gacataatg 
gcaaaattg acagcatcg gacctggtc cgggtcaat 
ataacgtaa ctggtctta gtgcaaaag gaaaagata 
cgccgccag ctgttagtt tgcacggtc gtcccaaag 
aagccaagc ctgaaaatt tccccgcac ctggggatt 
atgatcctg ataaagccg agaaagtta ttcacgaca 
acgcaccgc gatatactt ttggtttaa agacaaatg 
tcgaaaaac cgttggaca caccagcac ccaatgttt 
acaatgtac cagaatccg aaaaaaaat tgcatgaac 
attccccaa aatacagtt tcggactta aaactccaa 
tcgaaaaac catctgaca caccggcgc ccaatgttt 
acaacgtcc caacggccg acaaggttt tacatgaaa 
attctccca aatacagtt ttggatgca aggttcagt 
tggaaaagc cttcggaaa cacccgctc ccaatgtat 
acaatgttc caaatgccg ataaagtgt tacacgaga 
attccccta gatacagtt ttggcaaca aagttcaat 
tggaaaagc catcggata cccctgctc caaatgttt 
acaacgtgc cgacttccg aaaagatcc tacacgata 
attcaccta aatacagtt ttggaaata aggttcaac 
tggaaaaac ctttggaca ctcctgcac ccaatgtgt 
acaacattc cgccagttg ttggtgaca aaaaatccg 
cacctgctt acaccatat ctggaagaa acaaggacc 
cggttgacg aacgcgtaa aaaatccgg gtccaggcc 
aatataaca acgtcaatc cggaaagct acaaaactc 
attcgcctg catacacca taagtgggc gaacaaata 
ttccagttg attccacaa ctccagggc ccggagtct 
attctccgg aaaaggtgg atctcgatt atcatcctg 
cacacagtt ttggcatca ggcactccc aatacgcag 
agcattatt aaaaaaata aagttttca tcaaccgca 
gtatacgtt agttaacat cgttcacaa taattattg 
tgaagcaga aagcgaatc tgctttttt aattaaaag 
ctctctagg tacatcgta gttcctaaa ctgtgcaca 
attattgga tttgccaag cttatgaaa acgcttata 
atttaaccg cactataat tgcgaaaag ttttattca 
tgttggtag cgttaaccc actgttatg accggtttt 
agtttaatt agacaaagc gactaattt ttgttttat 
tgccatttt tttcaatgc tctctagcg cgtaatcta 
cacaagaga acaaccagc gattatata catttataa 
ttaaaataa ccatgtatt tgtcttttt tattaaata 
aagttttat atattga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY