Status
Please wait ...
Gene information
General information
TC008292 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8049665..8052387
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008292
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008292 RA
      MNTHLFSFL CVVFLIVLN VKCYYYEEQ KPIYLTASV 
      GDYVIFNCN VDFPQSIPI PYMLNWKKD GKTIYSWYN 
      GVITSMDPF VGRVDLLNP LNHAERRYG RGSVNLTSI 
      RDSDGGWYE CQVLFPNRT PSSRNNGTW FHLTVNGGN 
      LLAIPPINQ TTLEGDEAK FVCLAKDPE LKVYWYKDG 
      IPLREYQDL MQRSYVTKE NTLVISPTD MGDLGEYEC 
      EVRNVDGEK QTAKAFLNV QYKAKVIFA PPEIHLPYG 
      RPALIDCHF RANPPLTNL RWEKDGFLF DPYNIQGVF 
      YRRNGSLYF TKVDETHSG RYTCTPFND LGTQGPSPP 
      ILVTVQRPP IFIITPHNL YLRKLGETI EMPCDARDG 
      ENGHKPIIV WYKKDGTSL PVGRYSIRD GNLTIINIQ 
      EEDRGLYQC SATNKAATI TAETELLVE NIPSNAPTN 
      LTAISSSSS VHLTWAPGR QKSNVEYSV WYKAVEGSD 
      WKTHQVLSS RTLEATITG LNSGREYEF MVLCKDDNG 
      DGLFSKSVR VWTKGLDGE TDKFRGNSF PPVGYPRNI 
      SVYQTENGF MVTWEAPEY GKENLKFYI VRWFQGPED 
      YLFGSAETR NTSYLLTSL SEGVEYNIQ VVAVSFDDQ 
      QSVSERITL LVPGYKKIR AVSTGIITG FAFLVVAFV 
      GVYYARKKW CSSYSKN
    • >TC008292 RB
      MNTHLFSFL CVVFLIVLN VKCYYYEEQ KPIYLTASV 
      GDYVIFNCN VDFPQSIPI PYMLNWKKD GKTIYSWYN 
      GVITSMDPF VGRVDLLNP LNHAERRYG RGSVNLTSI 
      RDSDGGWYE CQVLFPNRT PSSRNNGTW FHLTVNGGN 
      LLAIPPINQ TTLEGDEAK FVCLAKDPE LKVYWYKDG 
      IPLREYQDL MQRSYVTKE NTLVISPTD MGDLGEYEC 
      EVRNVDGEK QTAKAFLNV QYKAKVIFA PPEIHLPYG 
      RPALIDCHF RANPPLTNL RWEKDGFLF DPYNIQGVF 
      YRRNGSLYF TKVDETHSG RYTCTPFND LGTQGPSPP 
      ILVTVQRPP IFIITPHNL YLRKLGETI EMPCDARDG 
      ENGHKPIIV WYKKDGTSL PVGRYSIRD GNLTIINIQ 
      EEDRGLYQC SATNKAATI TAETELLVE NIPSNAPTN 
      LTAISSSSS VHLTWAPGR QKSNVEYSV WYKAVEGSD 
      WKTHQVLSS RTLEATITG LNSGREYEF MVLCKDDNG 
      DGLFSKSVR VWTKGLDGE TDKFRGNSF PPVGYPRNI 
      SVYQTENGF MVTWEAPEY GKENLKFYI VRWFQGPED 
      YLFGSAETR NTSYLLTSL SEGVEYNIQ VVAVSFDDQ 
      QSVSERITL LVPGYKKIR AVSTGIITG FAFLVVAFV 
      GVYYARKKW CSSYSKNVK GSK
    CDS sequence
    • >TC008292 RA
      atgaatact catctcttc agtttttta tgcgtagtg 
      tttttaatt gtgctaaac gtgaaatgt tactattac 
      gaagaacag aaacctatt tatttaacg gcatcggtc 
      ggtgattac gtgattttt aactgcaac gtcgatttt 
      ccgcaaagc atccccatc ccctacatg ctcaactgg 
      aaaaaagac gggaagaca atttattca tggtacaat 
      ggggtgatc acctcaatg gacccattt gtgggccgc 
      gtggacctt ctcaaccct ttaaaccac gctgaacgt 
      cgatacgga cgaggttcc gttaattta acatcgatt 
      cgagactcg gacgggggt tggtacgag tgtcaggtc 
      ttatttccc aacagaacc cccagttcc aggaacaat 
      ggtacttgg ttccacctt acagtcaat ggtggaaat 
      ttactcgcg atccctcca atcaaccaa acaacgctg 
      gaaggggat gaagccaaa ttcgtatgt ttggcgaag 
      gacccagaa ctgaaggtt tattggtac aaagatggg 
      attcccttg cgggagtat caagaccta atgcaacgg 
      tcgtacgtt acgaaagaa aatactctc gttataagc 
      ccgacagat atgggggat ttgggcgaa tacgaatgc 
      gaagtaagg aatgtggat ggggaaaaa caaactgcc 
      aaagcgttc ctgaacgta caatataaa gcgaaggtt 
      attttcgcc ccgcctgaa atccacctg ccctatggt 
      cgtccagct ttgattgac tgccatttc agggcaaac 
      ccacctttg accaacctt cgttgggaa aaggatggg 
      tttctcttc gacccatac aacatacag ggtgttttc 
      taccgacgg aacggtagt ttgtatttc accaaagtt 
      gacgaaaca cacagtgga cggtacact tgtacccca 
      ttcaatgat ttaggcacc caaggcccc tcacccccg 
      atccttgta accgtccaa cgcccccct attttcata 
      ataaccccc cataattta tatttgcga aaactagga 
      gaaacgatt gaaatgcca tgtgatgcc cgggatggg 
      gaaaacgga cacaaacca ataatagtt tggtacaaa 
      aaagacgga acgtcccta ccagtgggc cgatactca 
      ataagagac gggaatttg acgataatt aacatccag 
      gaggaagat agagggttg tatcagtgc tctgcgact 
      aacaaggcg gccactatt accgcagaa acggaacta 
      ttggtggaa aatattccc tcaaacgct ccaacaaat 
      ctaacagcg atttcgtca agttcttcg gtgcatttg 
      acttgggcc cccggacgc caaaaatcg aatgttgaa 
      tatagcgtc tggtacaaa gctgtggag ggttcagat 
      tggaaaaca caccaagtt ttgtcgtct aggactttg 
      gaagcgacc atcactggt ctaaactct ggtagagaa 
      tacgagttc atggtgttg tgtaaggac gacaacggc 
      gatggcctt tttagcaag tcagtgcga gtgtggacc 
      aaaggcttg gacggggaa acagacaag tttagaggc 
      aattctttc ccccctgtg ggttatccc aggaacatt 
      tcagtttat caaacggaa aacggtttt atggtgaca 
      tgggaagcg cctgagtat ggcaaagaa aacctcaaa 
      ttttacatt gtgaggtgg tttcaaggc cctgaagat 
      tatctcttc gggtctgca gaaacccga aatacgtca 
      tacttgtta acaagtttg tccgaaggt gtcgaatac 
      aacattcaa gtcgtagct gtgtctttc gacgaccaa 
      caatcagtc agtgaaaga attacgctg cttgtgccc 
      ggttataaa aaaattcgg gccgtttca actgggatt 
      ataaccggc tttgctttc ctagttgtg gcatttgtt 
      ggagtctat tacgctcgg aaaaaatgg tgcagttcc 
      tattcgaaa aat
    • >TC008292 RB
      atgaatact catctcttc agtttttta tgcgtagtg 
      tttttaatt gtgctaaac gtgaaatgt tactattac 
      gaagaacag aaacctatt tatttaacg gcatcggtc 
      ggtgattac gtgattttt aactgcaac gtcgatttt 
      ccgcaaagc atccccatc ccctacatg ctcaactgg 
      aaaaaagac gggaagaca atttattca tggtacaat 
      ggggtgatc acctcaatg gacccattt gtgggccgc 
      gtggacctt ctcaaccct ttaaaccac gctgaacgt 
      cgatacgga cgaggttcc gttaattta acatcgatt 
      cgagactcg gacgggggt tggtacgag tgtcaggtc 
      ttatttccc aacagaacc cccagttcc aggaacaat 
      ggtacttgg ttccacctt acagtcaat ggtggaaat 
      ttactcgcg atccctcca atcaaccaa acaacgctg 
      gaaggggat gaagccaaa ttcgtatgt ttggcgaag 
      gacccagaa ctgaaggtt tattggtac aaagatggg 
      attcccttg cgggagtat caagaccta atgcaacgg 
      tcgtacgtt acgaaagaa aatactctc gttataagc 
      ccgacagat atgggggat ttgggcgaa tacgaatgc 
      gaagtaagg aatgtggat ggggaaaaa caaactgcc 
      aaagcgttc ctgaacgta caatataaa gcgaaggtt 
      attttcgcc ccgcctgaa atccacctg ccctatggt 
      cgtccagct ttgattgac tgccatttc agggcaaac 
      ccacctttg accaacctt cgttgggaa aaggatggg 
      tttctcttc gacccatac aacatacag ggtgttttc 
      taccgacgg aacggtagt ttgtatttc accaaagtt 
      gacgaaaca cacagtgga cggtacact tgtacccca 
      ttcaatgat ttaggcacc caaggcccc tcacccccg 
      atccttgta accgtccaa cgcccccct attttcata 
      ataaccccc cataattta tatttgcga aaactagga 
      gaaacgatt gaaatgcca tgtgatgcc cgggatggg 
      gaaaacgga cacaaacca ataatagtt tggtacaaa 
      aaagacgga acgtcccta ccagtgggc cgatactca 
      ataagagac gggaatttg acgataatt aacatccag 
      gaggaagat agagggttg tatcagtgc tctgcgact 
      aacaaggcg gccactatt accgcagaa acggaacta 
      ttggtggaa aatattccc tcaaacgct ccaacaaat 
      ctaacagcg atttcgtca agttcttcg gtgcatttg 
      acttgggcc cccggacgc caaaaatcg aatgttgaa 
      tatagcgtc tggtacaaa gctgtggag ggttcagat 
      tggaaaaca caccaagtt ttgtcgtct aggactttg 
      gaagcgacc atcactggt ctaaactct ggtagagaa 
      tacgagttc atggtgttg tgtaaggac gacaacggc 
      gatggcctt tttagcaag tcagtgcga gtgtggacc 
      aaaggcttg gacggggaa acagacaag tttagaggc 
      aattctttc ccccctgtg ggttatccc aggaacatt 
      tcagtttat caaacggaa aacggtttt atggtgaca 
      tgggaagcg cctgagtat ggcaaagaa aacctcaaa 
      ttttacatt gtgaggtgg tttcaaggc cctgaagat 
      tatctcttc gggtctgca gaaacccga aatacgtca 
      tacttgtta acaagtttg tccgaaggt gtcgaatac 
      aacattcaa gtcgtagct gtgtctttc gacgaccaa 
      caatcagtc agtgaaaga attacgctg cttgtgccc 
      ggttataaa aaaattcgg gccgtttca actgggatt 
      ataaccggc tttgctttc ctagttgtg gcatttgtt 
      ggagtctat tacgctcgg aaaaaatgg tgcagttcc 
      tattcgaaa aatgtaaaa ggaagtaaa taa
    mRNA sequence
    • >TC008292 RA
      tccacgtac attcaaaaa tgaatactc atctcttca 
      gttttttat gcgtagtgt ttttaattg tgctaaacg 
      tgaaatgtt actattacg aagaacaga aacctattt 
      atttaacgg catcggtcg gtgattacg tgattttta 
      actgcaacg tcgattttc cgcaaagca tccccatcc 
      cctacatgc tcaactgga aaaaagacg ggaagacaa 
      tttattcat ggtacaatg gggtgatca cctcaatgg 
      acccatttg tgggccgcg tggaccttc tcaaccctt 
      taaaccacg ctgaacgtc gatacggac gaggttccg 
      ttaatttaa catcgattc gagactcgg acgggggtt 
      ggtacgagt gtcaggtct tatttccca acagaaccc 
      ccagttcca ggaacaatg gtacttggt tccacctta 
      cagtcaatg gtggaaatt tactcgcga tccctccaa 
      tcaaccaaa caacgctgg aaggggatg aagccaaat 
      tcgtatgtt tggcgaagg acccagaac tgaaggttt 
      attggtaca aagatggga ttcccttgc gggagtatc 
      aagacctaa tgcaacggt cgtacgtta cgaaagaaa 
      atactctcg ttataagcc cgacagata tgggggatt 
      tgggcgaat acgaatgcg aagtaagga atgtggatg 
      gggaaaaac aaactgcca aagcgttcc tgaacgtac 
      aatataaag cgaaggtta ttttcgccc cgcctgaaa 
      tccacctgc cctatggtc gtccagctt tgattgact 
      gccatttca gggcaaacc cacctttga ccaaccttc 
      gttgggaaa aggatgggt ttctcttcg acccataca 
      acatacagg gtgttttct accgacgga acggtagtt 
      tgtatttca ccaaagttg acgaaacac acagtggac 
      ggtacactt gtaccccat tcaatgatt taggcaccc 
      aaggcccct cacccccga tccttgtaa ccgtccaac 
      gccccccta ttttcataa taacccccc ataatttat 
      atttgcgaa aactaggag aaacgattg aaatgccat 
      gtgatgccc gggatgggg aaaacggac acaaaccaa 
      taatagttt ggtacaaaa aagacggaa cgtccctac 
      cagtgggcc gatactcaa taagagacg ggaatttga 
      cgataatta acatccagg aggaagata gagggttgt 
      atcagtgct ctgcgacta acaaggcgg ccactatta 
      ccgcagaaa cggaactat tggtggaaa atattccct 
      caaacgctc caacaaatc taacagcga tttcgtcaa 
      gttcttcgg tgcatttga cttgggccc ccggacgcc 
      aaaaatcga atgttgaat atagcgtct ggtacaaag 
      ctgtggagg gttcagatt ggaaaacac accaagttt 
      tgtcgtcta ggactttgg aagcgacca tcactggtc 
      taaactctg gtagagaat acgagttca tggtgttgt 
      gtaaggacg acaacggcg atggccttt ttagcaagt 
      cagtgcgag tgtggacca aaggcttgg acggggaaa 
      cagacaagt ttagaggca attctttcc cccctgtgg 
      gttatccca ggaacattt cagtttatc aaacggaaa 
      acggtttta tggtgacat gggaagcgc ctgagtatg 
      gcaaagaaa acctcaaat tttacattg tgaggtggt 
      ttcaaggcc ctgaagatt atctcttcg ggtctgcag 
      aaacccgaa atacgtcat acttgttaa caagtttgt 
      ccgaaggtg tcgaataca acattcaag tcgtagctg 
      tgtctttcg acgaccaac aatcagtca gtgaaagaa 
      ttacgctgc ttgtgcccg gttataaaa aaattcggg 
      ccgtttcaa ctgggatta taaccggct ttgctttcc 
      tagttgtgg catttgttg gagtctatt acgctcgga 
      aaaaatggt gcagttcct attcgaaaa attaaaaac 
      aaaaaatgt gacaaaaat attgttata attattggg 
      gatatcgaa cgacactac caaatgtaa aaagaatat 
      tatgttcat gactcgaaa attgttttg agttagtaa 
      gtaggtata ttgtagctt atatttgtt tttagaaaa 
      aataaaggt tttgttttt ag
    • >TC008292 RB
      tccacgtac attcaaaaa tgaatactc atctcttca 
      gttttttat gcgtagtgt ttttaattg tgctaaacg 
      tgaaatgtt actattacg aagaacaga aacctattt 
      atttaacgg catcggtcg gtgattacg tgattttta 
      actgcaacg tcgattttc cgcaaagca tccccatcc 
      cctacatgc tcaactgga aaaaagacg ggaagacaa 
      tttattcat ggtacaatg gggtgatca cctcaatgg 
      acccatttg tgggccgcg tggaccttc tcaaccctt 
      taaaccacg ctgaacgtc gatacggac gaggttccg 
      ttaatttaa catcgattc gagactcgg acgggggtt 
      ggtacgagt gtcaggtct tatttccca acagaaccc 
      ccagttcca ggaacaatg gtacttggt tccacctta 
      cagtcaatg gtggaaatt tactcgcga tccctccaa 
      tcaaccaaa caacgctgg aaggggatg aagccaaat 
      tcgtatgtt tggcgaagg acccagaac tgaaggttt 
      attggtaca aagatggga ttcccttgc gggagtatc 
      aagacctaa tgcaacggt cgtacgtta cgaaagaaa 
      atactctcg ttataagcc cgacagata tgggggatt 
      tgggcgaat acgaatgcg aagtaagga atgtggatg 
      gggaaaaac aaactgcca aagcgttcc tgaacgtac 
      aatataaag cgaaggtta ttttcgccc cgcctgaaa 
      tccacctgc cctatggtc gtccagctt tgattgact 
      gccatttca gggcaaacc cacctttga ccaaccttc 
      gttgggaaa aggatgggt ttctcttcg acccataca 
      acatacagg gtgttttct accgacgga acggtagtt 
      tgtatttca ccaaagttg acgaaacac acagtggac 
      ggtacactt gtaccccat tcaatgatt taggcaccc 
      aaggcccct cacccccga tccttgtaa ccgtccaac 
      gccccccta ttttcataa taacccccc ataatttat 
      atttgcgaa aactaggag aaacgattg aaatgccat 
      gtgatgccc gggatgggg aaaacggac acaaaccaa 
      taatagttt ggtacaaaa aagacggaa cgtccctac 
      cagtgggcc gatactcaa taagagacg ggaatttga 
      cgataatta acatccagg aggaagata gagggttgt 
      atcagtgct ctgcgacta acaaggcgg ccactatta 
      ccgcagaaa cggaactat tggtggaaa atattccct 
      caaacgctc caacaaatc taacagcga tttcgtcaa 
      gttcttcgg tgcatttga cttgggccc ccggacgcc 
      aaaaatcga atgttgaat atagcgtct ggtacaaag 
      ctgtggagg gttcagatt ggaaaacac accaagttt 
      tgtcgtcta ggactttgg aagcgacca tcactggtc 
      taaactctg gtagagaat acgagttca tggtgttgt 
      gtaaggacg acaacggcg atggccttt ttagcaagt 
      cagtgcgag tgtggacca aaggcttgg acggggaaa 
      cagacaagt ttagaggca attctttcc cccctgtgg 
      gttatccca ggaacattt cagtttatc aaacggaaa 
      acggtttta tggtgacat gggaagcgc ctgagtatg 
      gcaaagaaa acctcaaat tttacattg tgaggtggt 
      ttcaaggcc ctgaagatt atctcttcg ggtctgcag 
      aaacccgaa atacgtcat acttgttaa caagtttgt 
      ccgaaggtg tcgaataca acattcaag tcgtagctg 
      tgtctttcg acgaccaac aatcagtca gtgaaagaa 
      ttacgctgc ttgtgcccg gttataaaa aaattcggg 
      ccgtttcaa ctgggatta taaccggct ttgctttcc 
      tagttgtgg catttgttg gagtctatt acgctcgga 
      aaaaatggt gcagttcct attcgaaaa atgtaaaag 
      gaagtaaat aagacgatc gttccgttc attgcaatg 
      ttataattt cagtaaaaa caaaaaatg tgacaaaaa 
      tattgttat aattattgg ggatatcga acgacacta 
      ccaaatgta aaaagaata ttatgttca tgactcgaa 
      aattgtttt gagttagta agtaggtat attgtagct 
      tatatttgt ttttagaaa aaataaagg ttttgtttt 
      tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY