| General information | |||
| TC008278 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 7663925..7666923 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008278 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04455 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC008278 RA
MSVKILLSF LSLIVCGSC QYRYYSYQY PYYGNYGYS NVRNGPYSY QPSPCRSSC GNVQPVNNC QYFPCYQQR SYNSYNLPY PYYQTSNNN YKTVNRNSN SPEPYGRFY QNLYNYQQP SQQDPYANV PTEIIDDDE FEKFMQKIP KLPKATTSS HSNYTVSQG AATSNSVVE TKPIRATIN RSLDPSVQN TPIRLDFLN GEPINADQN NEATTTEET EVTEVVETT TIPATTTEE TEDVAPVKN WFS
- >TC008278 RB
MKVKVSRRR EMSVKILLS FLSLIVCGS CQYRYYSYQ YPYYGNYGY SNVRNGPYS YQPSPCRSS CGNVQPVNN CQYFPCYQQ RSYNSYNLP YPYYQTSNN NYKTVNRNS NSPEPYGRF YQNLYNYQQ PSQQDPYAN VPTEIIDDD EFEKFMQKI PKLPKATTS SHSNYTVSQ GAATSNSVV ETKPIRATI NRSLDPSVQ NTPIRLDFL NGEPINADQ NNEATTTEE TEVTEVVET TTIPATTTE ETEDVAPVK NWFS
- >TC008278 RA
atgagtgtc aaaattttg ctaagtttt ctcagtttg attgtttgt ggctcttgc cagtatcga tactattca tatcagtac ccttactat ggcaattac ggatacagc aatgtaaga aatggacct tattcttat caaccttcg ccgtgtaga tcatcctgc ggtaacgtg caaccggtt aacaattgt caatacttt ccctgttat cagcaacgg tcttacaac agctacaac ttgccctac ccttactac caaaccagt aataacaat tacaaaaca gtcaaccgt aattcaaat tcgccagaa ccttacgga agattttat cagaacttg tacaattac caacaacct agccaacaa gatccttac gcaaatgtc ccgacggag ataatagac gacgacgag tttgaaaag tttatgcaa aaaattccc aaactacca aaagcgaca acctcatct cacagtaac tacacagtg agccaaggt gctgcgact agcaattct gttgtggag accaaacct ataagggcc acaataaac agaagtctt gacccttcc gtacaaaat acacccatc cggcttgat ttcttaaat ggagaaccc atcaacgct gaccaaaac aacgaagca actaccact gaagaaaca gaagtaacg gaagtggtc gaaacaaca accattcct gcaactacc accgaagaa accgaggat gtggcacct gtcaaaaac tggttttct tag
- >TC008278 RB
atgaaagta aaagtaagt agacgacga gagatgagt gtcaaaatt ttgctaagt tttctcagt ttgattgtt tgtggctct tgccagtat cgatactat tcatatcag tacccttac tatggcaat tacggatac agcaatgta agaaatgga ccttattct tatcaacct tcgccgtgt agatcatcc tgcggtaac gtgcaaccg gttaacaat tgtcaatac tttccctgt tatcagcaa cggtcttac aacagctac aacttgccc tacccttac taccaaacc agtaataac aattacaaa acagtcaac cgtaattca aattcgcca gaaccttac ggaagattt tatcagaac ttgtacaat taccaacaa cctagccaa caagatcct tacgcaaat gtcccgacg gagataata gacgacgac gagtttgaa aagtttatg caaaaaatt cccaaacta ccaaaagcg acaacctca tctcacagt aactacaca gtgagccaa ggtgctgcg actagcaat tctgttgtg gagaccaaa cctataagg gccacaata aacagaagt cttgaccct tccgtacaa aatacaccc atccggctt gatttctta aatggagaa cccatcaac gctgaccaa aacaacgaa gcaactacc actgaagaa acagaagta acggaagtg gtcgaaaca acaaccatt cctgcaact accaccgaa gaaaccgag gatgtggca cctgtcaaa aactggttt tcttag
- >TC008278 RA
ttcactata taaaagtgc attttttta atacattta gtagaatac agaagagta acaatgaaa attgcttcg atatttttg gttgctttt ctctgcact tttcaaaca agtgcagtg gaattgaag ccttccaat accgacatt ataagtgga gtaattgat gatgtagcc gatatagca aacggtgtt gtatgtctt gtagatgaa gtgactgga attttgtta ccaaatcag gacagttcg tgttataat gacgaaaat accaacgat gattgcggt gatgagtct ccatcctct acagcacca tgaattttg catacattt ttgtttaac caaaatgta ttgcttttt aaagaataa aattaatta aataatcat gtattaaat tgtaaaagc aacccacaa tatagtaac gttaataaa atctaaaat taaattatt atcattact ctcgctaat ctttttcca gaagttcaa tacagttgt tttgtctga aattacttt ttcctctgt ctgaatgtc atattttaa tgggtttta aaactataa catatgttc cgaccttca tgtacggga aaatgataa taaggaaaa aataaacat ttctttttt ttctaaaca aatgtgcgc ctaattttc ggtaagtca acaaatctt aaattaatg ttcacgatt ttactattt tttagttaa aaaacatga ttgcattgc taagaattt taacaattt atgtttgtt ttgtgttat tttgtaata ttatcagtt gtgacgaag atgcaatgt tatattccg atgaattta gaaaaatgc aagctgaca cgaccacca tcaaaggta actggcgca cttgtgcga tgaaaaata caaatgttc ttgcagttg ttttggtgg agaaatcgt tagttcgtg ttccttgcc aagcaccct ttaagttgg ttaacagga agtgtagat atgtttggt gattagtgt gcactattt aatagtttg aactttgtt ccgcgtctt gttcattgt tcttttggt ctttccttg tacccattt aacattttt tatcttgta gctattctc tgatagaac atattgtgt aacggggct agtgaggtc actcagata ataattttt gaaagtttc agttcctac atgaaagta aaagtaagt agacgacga gagatgagt gtcaaaatt ttgctaagt tttctcagt ttgattgtt tgtggctct tgccagtat cgatactat tcatatcag tacccttac tatggcaat tacggatac agcaatgta agaaatgga ccttattct tatcaacct tcgccgtgt agatcatcc tgcggtaac gtgcaaccg gttaacaat tgtcaatac tttccctgt tatcagcaa cggtcttac aacagctac aacttgccc tacccttac taccaaacc agtaataac aattacaaa acagtcaac cgtaattca aattcgcca gaaccttac ggaagattt tatcagaac ttgtacaat taccaacaa cctagccaa caagatcct tacgcaaat gtcccgacg gagataata gacgacgac gagtttgaa aagtttatg caaaaaatt cccaaacta ccaaaagcg acaacctca tctcacagt aactacaca gtgagccaa ggtgctgcg actagcaat tctgttgtg gagaccaaa cctataagg gccacaata aacagaagt cttgaccct tccgtacaa aatacaccc atccggctt gatttctta aatggagaa cccatcaac gctgaccaa aacaacgaa gcaactacc actgaagaa acagaagta acggaagtg gtcgaaaca acaaccatt cctgcaact accaccgaa gaaaccgag gatgtggca cctgtcaaa aactggttt tcttagaaa tagtttgta attttgtat cgtttaata aatacgttt tttatgtcc acaatctca aattattat tttggtgat ctctgacga cctttatat atcttgaat cgatataaa tacataagt gcaattttg ttcaagtca agttagttg aattcatcg tgcaataaa aatgaaaat tgtattgct tttcgtagc aactttttt gttgtgttg ttcagaatg taaaccaga cgtaaagac ggtattcga tacttcaaa ttcttgtcc aaatgggaa gttattggc ccataacgg ggaatgcat cacgccatt tatgttgaa gatgaccat tccagatgc ggtggtcaa tgcattgtc taccacaga agcaacgta agaatttgt aattgtaaa tcgttcttg ttattgact tttccagac agaggaaag taaatactg tataaatcg tttgcttgc gcattttca cgtggaaat ttctgtttt aataaattg caataaaac ggtaatttg attgtaaca gtgcaaaca ctgttatca catcttcca acaataata aaagctact tgtcgtcag aagccttac aacgtatca aaagtctgg cggcacagg tgaacatgt cgaaagttt gtctattgt tactgattg tgatgtcca tcacatgtg tattaagtg atgtggatg gggattacc gtcaggtct tcggtcacg aaaattcta acataaatc cgtatttca aaaatatca tccgcatcc gttgcaaac ctggctatg tccgaattc gcgataagt gtgtcaatt ttttaaaga atgaagatg atgtaagta tgggtggaa agaaaggga attatcaaa tgtggttta ccaaggtct ttggccgtg gttacgagt ctagcagcc cagatcttc atccaaaaa aaatcttga aagttcggt gcaaatctg gctatgctc tagttaagg gtaaatgtg tcaaaactg ttaaaaatg aagattgat gtaagtatt ggtttcaat aagaaactt atcgacaaa at
- >TC008278 RB
ttcactata taaaagtgc attttttta atacattta gtagaatac agaagagta acaatgaaa attgcttcg atatttttg gttgctttt ctctgcact tttcaaaca agtgcagtg gaattgaag ccttccaat accgacatt ataagtgga gtaattgat gatgtagcc gatatagca aacggtgtt gtatgtctt gtagatgaa gtgactgga attttgtta ccaaatcag gacagttcg tgttataat gacgaaaat accaacgat gattgcggt ggtaattta cctttcttc taaataaca gttattaaa taaaaaaaa atatttcta gatgagtct ccatcctct acagcacca tgaattttg catacattt ttgtttaac caaaatgta ttgcttttt aaagaataa aattaatta aataatcat gtattaaat tgtaaaagc aacccacaa tatagtaac gttaataaa atctaaaat taaattatt atcattact ctcgctaat ctttttcca gaagttcaa tacagttgt tttgtctga aattacttt ttcctctgt ctgaatgtc atattttaa tgggtttta aaactataa catatgttc cgaccttca tgtacggga aaatgataa taaggaaaa aataaacat ttctttttt ttctaaaca aatgtgcgc ctaattttc gttaaaaaa catgattgc attgctaag aattttaac aatttatgt ttgttttgt gttattttg taatattat cagttgtga cgaagatgc aatgttata ttccgatga atttagaaa aatgcaagc tgacacgac caccatcaa aggtaactg gcgcacttg tgcgatgaa aaatacaaa tgttcttgc agttgtttt ggtggagaa atcgttagt tcgtgttcc ttgccaagc accctttaa gttggttaa caggaagtg tagatatgt ttggtgatt agtgtgcac tatttaata gtttgaact ttgttccgc gtcttgttc attgttctt ttggtcttt ccttgtacc catttaaca ttttttatc ttgtagcta ttctctgat agaacatat tgtgtaacg gggctagtg aggtcactc agataataa tttttgaaa gtttcagtt cctacatga aagtaaaag taagtagac gacgagaga tgagtgtca aaattttgc taagttttc tcagtttga ttgtttgtg gctcttgcc agtatcgat actattcat atcagtacc cttactatg gcaattacg gatacagca atgtaagaa atggacctt attcttatc aaccttcgc cgtgtagat catcctgcg gtaacgtgc aaccggtta acaattgtc aatactttc cctgttatc agcaacggt cttacaaca gctacaact tgccctacc cttactacc aaaccagta ataacaatt acaaaacag tcaaccgta attcaaatt cgccagaac cttacggaa gattttatc agaacttgt acaattacc aacaaccta gccaacaag atccttacg caaatgtcc cgacggaga taatagacg acgacgagt ttgaaaagt ttatgcaaa aaattccca aactaccaa aagcgacaa cctcatctc acagtaact acacagtga gccaaggtg ctgcgacta gcaattctg ttgtggaga ccaaaccta taagggcca caataaaca gaagtcttg acccttccg tacaaaata cacccatcc ggcttgatt tcttaaatg gagaaccca tcaacgctg accaaaaca acgaagcaa ctaccactg aagaaacag aagtaacgg aagtggtcg aaacaacaa ccattcctg caactacca ccgaagaaa ccgaggatg tggcacctg tcaaaaact ggttttctt agaaatagt ttgtaattt tgtatcgtt taataaata cgtttttta tgtccacaa tctcaaatt attattttg gtgatctct gacgacctt tatatatct tgaatcgat ataaataca taagtgcaa ttttgttca agtcaagtt agttgaatt catcgtgca ataaaaatg aaaattgta ttgcttttc gtagcaact tttttgttg tgttgttca gaatgtaaa ccagacgta aagacggta ttcgatact tcaaattct tgtccaaat gggaagtta ttggcccat aacggggaa tgcatcacg ccatttatg ttgaagatg accattcca gatgcggtg gtcaatgca ttgtctacc acagaagca acgtaagaa tttgtaatt gtaaatcgt tcttgttat tgacttttc cagacagag gaaagtaaa tactgtata aatcgtttg cttgcgcat tttcacgtg gaaatttct gttttaata aattgcaat aaaacggta atttgattg taacagtgc aaacactgt tatcacatc ttccaacaa taataaaag ctacttgtc gtcagaagc cttacaacg tatcaaaag tctggcggc acaggtgaa catgtcgaa agtttgtct attgttact gattgtgat gtccatcac atgtgtatt aagtgatgt ggatgggga ttaccgtca ggtcttcgg tcacgaaaa ttctaacat aaatccgta tttcaaaaa tatcatccg catccgttg caaacctgg ctatgtccg aattcgcga taagtgtgt caatttttt aaagaatga agatgatgt aagtatggg tggaaagaa agggaatta tcaaatgtg gtttaccaa ggtctttgg ccgtggtta cgagtctag cagcccaga tcttcatcc aaaaaaaat cttgaaagt tcggtgcaa atctggcta tgctctagt taagggtaa atgtgtcaa aactgttaa aaatgaaga ttgatgtaa gtattggtt tcaataaga aacttatcg acaaaat
