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Gene information
General information
TC008278 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7663925..7666923
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008278
      iBeetle screen iB_04455

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008278 RA
        MSVKILLSF LSLIVCGSC QYRYYSYQY PYYGNYGYS 
        NVRNGPYSY QPSPCRSSC GNVQPVNNC QYFPCYQQR 
        SYNSYNLPY PYYQTSNNN YKTVNRNSN SPEPYGRFY 
        QNLYNYQQP SQQDPYANV PTEIIDDDE FEKFMQKIP 
        KLPKATTSS HSNYTVSQG AATSNSVVE TKPIRATIN 
        RSLDPSVQN TPIRLDFLN GEPINADQN NEATTTEET 
        EVTEVVETT TIPATTTEE TEDVAPVKN WFS
      • >TC008278 RB
        MKVKVSRRR EMSVKILLS FLSLIVCGS CQYRYYSYQ 
        YPYYGNYGY SNVRNGPYS YQPSPCRSS CGNVQPVNN 
        CQYFPCYQQ RSYNSYNLP YPYYQTSNN NYKTVNRNS 
        NSPEPYGRF YQNLYNYQQ PSQQDPYAN VPTEIIDDD 
        EFEKFMQKI PKLPKATTS SHSNYTVSQ GAATSNSVV 
        ETKPIRATI NRSLDPSVQ NTPIRLDFL NGEPINADQ 
        NNEATTTEE TEVTEVVET TTIPATTTE ETEDVAPVK 
        NWFS
      CDS sequence
      • >TC008278 RA
        atgagtgtc aaaattttg ctaagtttt ctcagtttg 
        attgtttgt ggctcttgc cagtatcga tactattca 
        tatcagtac ccttactat ggcaattac ggatacagc 
        aatgtaaga aatggacct tattcttat caaccttcg 
        ccgtgtaga tcatcctgc ggtaacgtg caaccggtt 
        aacaattgt caatacttt ccctgttat cagcaacgg 
        tcttacaac agctacaac ttgccctac ccttactac 
        caaaccagt aataacaat tacaaaaca gtcaaccgt 
        aattcaaat tcgccagaa ccttacgga agattttat 
        cagaacttg tacaattac caacaacct agccaacaa 
        gatccttac gcaaatgtc ccgacggag ataatagac 
        gacgacgag tttgaaaag tttatgcaa aaaattccc 
        aaactacca aaagcgaca acctcatct cacagtaac 
        tacacagtg agccaaggt gctgcgact agcaattct 
        gttgtggag accaaacct ataagggcc acaataaac 
        agaagtctt gacccttcc gtacaaaat acacccatc 
        cggcttgat ttcttaaat ggagaaccc atcaacgct 
        gaccaaaac aacgaagca actaccact gaagaaaca 
        gaagtaacg gaagtggtc gaaacaaca accattcct 
        gcaactacc accgaagaa accgaggat gtggcacct 
        gtcaaaaac tggttttct tag
      • >TC008278 RB
        atgaaagta aaagtaagt agacgacga gagatgagt 
        gtcaaaatt ttgctaagt tttctcagt ttgattgtt 
        tgtggctct tgccagtat cgatactat tcatatcag 
        tacccttac tatggcaat tacggatac agcaatgta 
        agaaatgga ccttattct tatcaacct tcgccgtgt 
        agatcatcc tgcggtaac gtgcaaccg gttaacaat 
        tgtcaatac tttccctgt tatcagcaa cggtcttac 
        aacagctac aacttgccc tacccttac taccaaacc 
        agtaataac aattacaaa acagtcaac cgtaattca 
        aattcgcca gaaccttac ggaagattt tatcagaac 
        ttgtacaat taccaacaa cctagccaa caagatcct 
        tacgcaaat gtcccgacg gagataata gacgacgac 
        gagtttgaa aagtttatg caaaaaatt cccaaacta 
        ccaaaagcg acaacctca tctcacagt aactacaca 
        gtgagccaa ggtgctgcg actagcaat tctgttgtg 
        gagaccaaa cctataagg gccacaata aacagaagt 
        cttgaccct tccgtacaa aatacaccc atccggctt 
        gatttctta aatggagaa cccatcaac gctgaccaa 
        aacaacgaa gcaactacc actgaagaa acagaagta 
        acggaagtg gtcgaaaca acaaccatt cctgcaact 
        accaccgaa gaaaccgag gatgtggca cctgtcaaa 
        aactggttt tcttag
      mRNA sequence
      • >TC008278 RA
        ttcactata taaaagtgc attttttta atacattta 
        gtagaatac agaagagta acaatgaaa attgcttcg 
        atatttttg gttgctttt ctctgcact tttcaaaca 
        agtgcagtg gaattgaag ccttccaat accgacatt 
        ataagtgga gtaattgat gatgtagcc gatatagca 
        aacggtgtt gtatgtctt gtagatgaa gtgactgga 
        attttgtta ccaaatcag gacagttcg tgttataat 
        gacgaaaat accaacgat gattgcggt gatgagtct 
        ccatcctct acagcacca tgaattttg catacattt 
        ttgtttaac caaaatgta ttgcttttt aaagaataa 
        aattaatta aataatcat gtattaaat tgtaaaagc 
        aacccacaa tatagtaac gttaataaa atctaaaat 
        taaattatt atcattact ctcgctaat ctttttcca 
        gaagttcaa tacagttgt tttgtctga aattacttt 
        ttcctctgt ctgaatgtc atattttaa tgggtttta 
        aaactataa catatgttc cgaccttca tgtacggga 
        aaatgataa taaggaaaa aataaacat ttctttttt 
        ttctaaaca aatgtgcgc ctaattttc ggtaagtca 
        acaaatctt aaattaatg ttcacgatt ttactattt 
        tttagttaa aaaacatga ttgcattgc taagaattt 
        taacaattt atgtttgtt ttgtgttat tttgtaata 
        ttatcagtt gtgacgaag atgcaatgt tatattccg 
        atgaattta gaaaaatgc aagctgaca cgaccacca 
        tcaaaggta actggcgca cttgtgcga tgaaaaata 
        caaatgttc ttgcagttg ttttggtgg agaaatcgt 
        tagttcgtg ttccttgcc aagcaccct ttaagttgg 
        ttaacagga agtgtagat atgtttggt gattagtgt 
        gcactattt aatagtttg aactttgtt ccgcgtctt 
        gttcattgt tcttttggt ctttccttg tacccattt 
        aacattttt tatcttgta gctattctc tgatagaac 
        atattgtgt aacggggct agtgaggtc actcagata 
        ataattttt gaaagtttc agttcctac atgaaagta 
        aaagtaagt agacgacga gagatgagt gtcaaaatt 
        ttgctaagt tttctcagt ttgattgtt tgtggctct 
        tgccagtat cgatactat tcatatcag tacccttac 
        tatggcaat tacggatac agcaatgta agaaatgga 
        ccttattct tatcaacct tcgccgtgt agatcatcc 
        tgcggtaac gtgcaaccg gttaacaat tgtcaatac 
        tttccctgt tatcagcaa cggtcttac aacagctac 
        aacttgccc tacccttac taccaaacc agtaataac 
        aattacaaa acagtcaac cgtaattca aattcgcca 
        gaaccttac ggaagattt tatcagaac ttgtacaat 
        taccaacaa cctagccaa caagatcct tacgcaaat 
        gtcccgacg gagataata gacgacgac gagtttgaa 
        aagtttatg caaaaaatt cccaaacta ccaaaagcg 
        acaacctca tctcacagt aactacaca gtgagccaa 
        ggtgctgcg actagcaat tctgttgtg gagaccaaa 
        cctataagg gccacaata aacagaagt cttgaccct 
        tccgtacaa aatacaccc atccggctt gatttctta 
        aatggagaa cccatcaac gctgaccaa aacaacgaa 
        gcaactacc actgaagaa acagaagta acggaagtg 
        gtcgaaaca acaaccatt cctgcaact accaccgaa 
        gaaaccgag gatgtggca cctgtcaaa aactggttt 
        tcttagaaa tagtttgta attttgtat cgtttaata 
        aatacgttt tttatgtcc acaatctca aattattat 
        tttggtgat ctctgacga cctttatat atcttgaat 
        cgatataaa tacataagt gcaattttg ttcaagtca 
        agttagttg aattcatcg tgcaataaa aatgaaaat 
        tgtattgct tttcgtagc aactttttt gttgtgttg 
        ttcagaatg taaaccaga cgtaaagac ggtattcga 
        tacttcaaa ttcttgtcc aaatgggaa gttattggc 
        ccataacgg ggaatgcat cacgccatt tatgttgaa 
        gatgaccat tccagatgc ggtggtcaa tgcattgtc 
        taccacaga agcaacgta agaatttgt aattgtaaa 
        tcgttcttg ttattgact tttccagac agaggaaag 
        taaatactg tataaatcg tttgcttgc gcattttca 
        cgtggaaat ttctgtttt aataaattg caataaaac 
        ggtaatttg attgtaaca gtgcaaaca ctgttatca 
        catcttcca acaataata aaagctact tgtcgtcag 
        aagccttac aacgtatca aaagtctgg cggcacagg 
        tgaacatgt cgaaagttt gtctattgt tactgattg 
        tgatgtcca tcacatgtg tattaagtg atgtggatg 
        gggattacc gtcaggtct tcggtcacg aaaattcta 
        acataaatc cgtatttca aaaatatca tccgcatcc 
        gttgcaaac ctggctatg tccgaattc gcgataagt 
        gtgtcaatt ttttaaaga atgaagatg atgtaagta 
        tgggtggaa agaaaggga attatcaaa tgtggttta 
        ccaaggtct ttggccgtg gttacgagt ctagcagcc 
        cagatcttc atccaaaaa aaatcttga aagttcggt 
        gcaaatctg gctatgctc tagttaagg gtaaatgtg 
        tcaaaactg ttaaaaatg aagattgat gtaagtatt 
        ggtttcaat aagaaactt atcgacaaa at
      • >TC008278 RB
        ttcactata taaaagtgc attttttta atacattta 
        gtagaatac agaagagta acaatgaaa attgcttcg 
        atatttttg gttgctttt ctctgcact tttcaaaca 
        agtgcagtg gaattgaag ccttccaat accgacatt 
        ataagtgga gtaattgat gatgtagcc gatatagca 
        aacggtgtt gtatgtctt gtagatgaa gtgactgga 
        attttgtta ccaaatcag gacagttcg tgttataat 
        gacgaaaat accaacgat gattgcggt ggtaattta 
        cctttcttc taaataaca gttattaaa taaaaaaaa 
        atatttcta gatgagtct ccatcctct acagcacca 
        tgaattttg catacattt ttgtttaac caaaatgta 
        ttgcttttt aaagaataa aattaatta aataatcat 
        gtattaaat tgtaaaagc aacccacaa tatagtaac 
        gttaataaa atctaaaat taaattatt atcattact 
        ctcgctaat ctttttcca gaagttcaa tacagttgt 
        tttgtctga aattacttt ttcctctgt ctgaatgtc 
        atattttaa tgggtttta aaactataa catatgttc 
        cgaccttca tgtacggga aaatgataa taaggaaaa 
        aataaacat ttctttttt ttctaaaca aatgtgcgc 
        ctaattttc gttaaaaaa catgattgc attgctaag 
        aattttaac aatttatgt ttgttttgt gttattttg 
        taatattat cagttgtga cgaagatgc aatgttata 
        ttccgatga atttagaaa aatgcaagc tgacacgac 
        caccatcaa aggtaactg gcgcacttg tgcgatgaa 
        aaatacaaa tgttcttgc agttgtttt ggtggagaa 
        atcgttagt tcgtgttcc ttgccaagc accctttaa 
        gttggttaa caggaagtg tagatatgt ttggtgatt 
        agtgtgcac tatttaata gtttgaact ttgttccgc 
        gtcttgttc attgttctt ttggtcttt ccttgtacc 
        catttaaca ttttttatc ttgtagcta ttctctgat 
        agaacatat tgtgtaacg gggctagtg aggtcactc 
        agataataa tttttgaaa gtttcagtt cctacatga 
        aagtaaaag taagtagac gacgagaga tgagtgtca 
        aaattttgc taagttttc tcagtttga ttgtttgtg 
        gctcttgcc agtatcgat actattcat atcagtacc 
        cttactatg gcaattacg gatacagca atgtaagaa 
        atggacctt attcttatc aaccttcgc cgtgtagat 
        catcctgcg gtaacgtgc aaccggtta acaattgtc 
        aatactttc cctgttatc agcaacggt cttacaaca 
        gctacaact tgccctacc cttactacc aaaccagta 
        ataacaatt acaaaacag tcaaccgta attcaaatt 
        cgccagaac cttacggaa gattttatc agaacttgt 
        acaattacc aacaaccta gccaacaag atccttacg 
        caaatgtcc cgacggaga taatagacg acgacgagt 
        ttgaaaagt ttatgcaaa aaattccca aactaccaa 
        aagcgacaa cctcatctc acagtaact acacagtga 
        gccaaggtg ctgcgacta gcaattctg ttgtggaga 
        ccaaaccta taagggcca caataaaca gaagtcttg 
        acccttccg tacaaaata cacccatcc ggcttgatt 
        tcttaaatg gagaaccca tcaacgctg accaaaaca 
        acgaagcaa ctaccactg aagaaacag aagtaacgg 
        aagtggtcg aaacaacaa ccattcctg caactacca 
        ccgaagaaa ccgaggatg tggcacctg tcaaaaact 
        ggttttctt agaaatagt ttgtaattt tgtatcgtt 
        taataaata cgtttttta tgtccacaa tctcaaatt 
        attattttg gtgatctct gacgacctt tatatatct 
        tgaatcgat ataaataca taagtgcaa ttttgttca 
        agtcaagtt agttgaatt catcgtgca ataaaaatg 
        aaaattgta ttgcttttc gtagcaact tttttgttg 
        tgttgttca gaatgtaaa ccagacgta aagacggta 
        ttcgatact tcaaattct tgtccaaat gggaagtta 
        ttggcccat aacggggaa tgcatcacg ccatttatg 
        ttgaagatg accattcca gatgcggtg gtcaatgca 
        ttgtctacc acagaagca acgtaagaa tttgtaatt 
        gtaaatcgt tcttgttat tgacttttc cagacagag 
        gaaagtaaa tactgtata aatcgtttg cttgcgcat 
        tttcacgtg gaaatttct gttttaata aattgcaat 
        aaaacggta atttgattg taacagtgc aaacactgt 
        tatcacatc ttccaacaa taataaaag ctacttgtc 
        gtcagaagc cttacaacg tatcaaaag tctggcggc 
        acaggtgaa catgtcgaa agtttgtct attgttact 
        gattgtgat gtccatcac atgtgtatt aagtgatgt 
        ggatgggga ttaccgtca ggtcttcgg tcacgaaaa 
        ttctaacat aaatccgta tttcaaaaa tatcatccg 
        catccgttg caaacctgg ctatgtccg aattcgcga 
        taagtgtgt caatttttt aaagaatga agatgatgt 
        aagtatggg tggaaagaa agggaatta tcaaatgtg 
        gtttaccaa ggtctttgg ccgtggtta cgagtctag 
        cagcccaga tcttcatcc aaaaaaaat cttgaaagt 
        tcggtgcaa atctggcta tgctctagt taagggtaa 
        atgtgtcaa aactgttaa aaatgaaga ttgatgtaa 
        gtattggtt tcaataaga aacttatcg acaaaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY