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Gene information
General information
TC008244 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7187873..7192181
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008244
    iBeetle screen iB_04450

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008244 RA
      MELGVLKGP SELIASRVK AFKKCLRVA TDCLTNKDY 
      PNAEMIYGG ILADLHKDC IVEVLNLND LELFSLHIC 
      FAYSSIGAN YKSEQALVI LNSIHEPIK LPLVPYLKA 
      FIHISNDRL SFANDELQF CNSLLKDGY NFKSNDILN 
      EKIVPEISD EFLKLKLNG LKFICDLLL AREDNTNLK 
      RCFKENWLK IGTLCEPDF ESLTKNTVK ETPKEEKVT 
      KKTKKQTGA IKKMEKPAQ SNKSIAIQT APPKGINVS 
      VQTMEKDID KQLLAAVLE IKNRLNNIQ NKKDGEIKQ 
      LQKKNNELN EKLVATNST GEEKSPEFE FLKDQYLVL 
      EKKFEQTQK QNELLQKLL IEQMQQRYQ MQKSIYQMF 
      QEKCDYRFS YLSDLYQYL YPSDKPTPE ECKQEAFLW 
      KNTQDKVSE AKKLFEAYY KTVDDLIIA KKYAEVKIS 
      DWPGIPNIP NDIQDVIYR SFKSMVQFN KNIYSATRP 
      QQQYLSVLG NYGNFKEYV RKSNNYSNN YKSYHQDGR 
      TTGSPSYSQ TPPPPPPSN TNGYPPSGN GCPPPANQN 
      GYHPPSGHR YASPTPNNG YPPSSNGYP PSSNGYPPS 
      SNGYPPPPP NVYQPQPPN IRKQVSPPS PPRVNSVNV 
      YQLEQELLK FLQITFDSV PETDLLEAI CLVRDNNNG 
      GNLIGIPKQ VLVPEIKKA LKMKSAKNA WSNVSQPAW 
      HNDHSEHKC CICFEDFDP SNSHMLSCQ HEFHKQCIT 
      NWLKRQSAC PVCRVHAVI DEEFPPLKS
    CDS sequence
    • >TC008244 RA
      atggagtta ggcgtttta aagggtccc agcgaactt 
      atagcatct cgagtgaaa gctttcaaa aaatgcctc 
      agagtggcc actgattgt ctaacaaat aaagattat 
      ccaaacgct gaaatgatt tacggaggt attttggcc 
      gatttgcac aaagattgc attgttgag gttctgaat 
      ttgaacgat cttgagctt ttctctttg catatttgc 
      tttgcttat tcctcaatt ggagctaat tacaaatca 
      gaacaagct ttggttatt ttgaactcc atacatgaa 
      cctataaaa cttccattg gtgccttat ctcaaagcg 
      tttattcat atctcgaac gatagactg tcgtttgct 
      aatgacgag ttacagttt tgtaatagt ttattgaag 
      gatggttat aatttcaag tcaaatgat atattgaat 
      gagaagatc gtacctgaa ataagcgac gaattttta 
      aagttgaaa ctaaacgga ttgaaattt atttgtgat 
      ttgttgttg gctagagaa gataacacc aacttgaag 
      agatgtttt aaagaaaat tggttgaaa attggtact 
      ctttgtgaa cccgatttt gaaagttta acaaagaat 
      actgtaaaa gagacccca aaagaggaa aaggtcaca 
      aagaaaacc aaaaaacaa accggagca atcaaaaaa 
      atggaaaaa ccagcccaa agtaataaa agtattgct 
      atccaaact gctccccca aaagggata aacgtttct 
      gtccaaaca atggagaaa gacatcgat aagcaatta 
      ttggcagct gttcttgaa attaaaaat agattaaat 
      aacatacaa aacaagaaa gatggcgaa atcaaacaa 
      cttcagaag aaaaacaac gaacttaat gaaaaactt 
      gttgctaca aatagcacg ggggaggag aaatccccc 
      gagtttgaa tttttgaaa gaccagtat cttgtccta 
      gagaaaaaa tttgagcaa acacagaaa caaaacgaa 
      ctgttgcaa aaactgctc atcgagcaa atgcaacaa 
      agatatcaa atgcagaaa agtatctac caaatgttt 
      caagaaaaa tgtgactat aggttttca tacttgagc 
      gatctttac caatatttg taccctagc gataaacca 
      acgccagaa gaatgcaaa caggaagcg ttcttgtgg 
      aaaaataca caggataag gtttcagaa gcaaaaaaa 
      ctatttgag gcatattat aaaacagtt gatgaccta 
      ataattgca aaaaaatat gctgaagtt aaaataagc 
      gactggcct ggaataccc aatattcca aacgacatt 
      caagatgtt atttaccgc agttttaag agtatggtt 
      caatttaat aaaaacatt tacagtgct acgcgtcca 
      caacaacag tatctgtct gtgttgggt aattatgga 
      aacttcaaa gaatacgtg cgaaaatcc aataattac 
      tccaataac tacaaaagc taccatcaa gacggaaga 
      actacaggg tccccgtca tattcacaa actccacct 
      cctcctccc ccatccaat accaatgga tatcctcca 
      tctggaaat ggatgtcct cctcctgcc aaccaaaat 
      ggatatcat cctccatct ggccataga tatgcttcc 
      ccaaccccc aacaatgga tatcctccc tcctctaat 
      ggatatcct ccctcctct aatggatat cctccctcc 
      tctaatgga tatcctcct ccacccccc aacgtatat 
      cagccacag ccacctaat attaggaaa caggtgtcg 
      cccccgtca ccccctaga gttaattct gttaatgtg 
      tatcaacta gaacaagaa ttgcttaaa tttcttcaa 
      attactttc gatagcgtt cccgaaact gatttgcta 
      gaagcgatt tgcctagtt cgcgataac aataacggt 
      ggtaatctt ataggaatt cctaaacaa gtactagtt 
      cctgaaatt aagaaagct ttgaaaatg aaaagtgcg 
      aaaaatgca tggagcaat gtgtctcaa cctgcctgg 
      cacaacgat catagtgaa cataaatgc tgtatttgt 
      tttgaagat tttgatcct agtaacagc catatgcta 
      tcgtgtcag catgagttt cataaacag tgcattact 
      aactggctg aaacgacaa agtgcctgt ccggtgtgt 
      agagtgcac gccgttatt gatgaggag tttccacca 
      ctgaagtct taa
    mRNA sequence
    • >TC008244 RA
      atgtttttg accatttca attaacgtt tgtcctttt 
      gtttacgct caacgtcag tcgaattta aacaagttc 
      aaatttaat atcaaacag tcaaaattc tttccaaaa 
      aattgaata attgcatga aagatggag ttaggcgtt 
      ttaaagggt cccagcgaa cttatagca tctcgagtg 
      aaagctttc aaaaaatgc ctcagagtg gccactgat 
      tgtctaaca aataaagat tatccaaac gctgaaatg 
      atttacgga ggtattttg gccgatttg cacaaagat 
      tgcattgtt gaggttctg aatttgaac gatcttgag 
      cttttctct ttgcatatt tgctttgct tattcctca 
      attggagct aattacaaa tcagaacaa gctttggtt 
      attttgaac tccatacat gaacctata aaacttcca 
      ttggtgcct tatctcaaa gcgtttatt catatctcg 
      aacgataga ctgtcgttt gctaatgac gagttacag 
      ttttgtaat agtttattg aaggatggt tataatttc 
      aagtcaaat gatatattg aatgagaag atcgtacct 
      gaaataagc gacgaattt ttaaagttg aaactaaac 
      ggattgaaa tttatttgt gatttgttg ttggctaga 
      gaagataac accaacttg aagagatgt tttaaagaa 
      aattggttg aaaattggt actctttgt gaacccgat 
      tttgaaagt ttaacaaag aatactgta aaagagacc 
      ccaaaagag gaaaaggtc acaaagaaa accaaaaaa 
      caaaccgga gcaatcaaa aaaatggaa aaaccagcc 
      caaagtaat aaaagtatt gctatccaa actgctccc 
      ccaaaaggg ataaacgtt tctgtccaa acaatggag 
      aaagacatc gataagcaa ttattggca gctgttctt 
      gaaattaaa aatagatta aataacata caaaacaag 
      aaagatggc gaaatcaaa caacttcag aagaaaaac 
      aacgaactt aatgaaaaa cttgttgct acaaatagc 
      acgggggag gagaaatcc cccgagttt gaatttttg 
      aaagaccag tatcttgtc ctagagaaa aaatttgag 
      caaacacag aaacaaaac gaactgttg caaaaactg 
      ctcatcgag caaatgcaa caaagatat caaatgcag 
      aaaagtatc taccaaatg tttcaagaa aaatgtgac 
      tataggttt tcatacttg agcgatctt taccaatat 
      ttgtaccct agcgataaa ccaacgcca gaagaatgc 
      aaacaggaa gcgttcttg tggaaaaat acacaggat 
      aaggtttca gaagcaaaa aaactattt gaggcatat 
      tataaaaca gttgatgac ctaataatt gcaaaaaaa 
      tatgctgaa gttaaaata agcgactgg cctggaata 
      cccaatatt ccaaacgac attcaagat gttatttac 
      cgcagtttt aagagtatg gttcaattt aataaaaac 
      atttacagt gctacgcgt ccacaacaa cagtatctg 
      tctgtgttg ggtaattat ggaaacttc aaagaatac 
      gtgcgaaaa tccaataat tactccaat aactacaaa 
      agctaccat caagacgga agaactaca gggtccccg 
      tcatattca caaactcca cctcctcct cccccatcc 
      aataccaat ggatatcct ccatctgga aatggatgt 
      cctcctcct gccaaccaa aatggatat catcctcca 
      tctggccat agatatgct tccccaacc cccaacaat 
      ggatatcct ccctcctct aatggatat cctccctcc 
      tctaatgga tatcctccc tcctctaat ggatatcct 
      cctccaccc cccaacgta tatcagcca cagccacct 
      aatattagg aaacaggtg tcgcccccg tcaccccct 
      agagttaat tctgttaat gtgtatcaa ctagaacaa 
      gaattgctt aaatttctt caaattact ttcgatagc 
      gttcccgaa actgatttg ctagaagcg atttgccta 
      gttcgcgat aacaataac ggtggtaat cttatagga 
      attcctaaa caagtacta gttcctgaa attaagaaa 
      gctttgaaa atgaaaagt gcgaaaaat gcatggagc 
      aatgtgtct caacctgcc tggcacaac gatcatagt 
      gaacataaa tgctgtatt tgttttgaa gattttgat 
      cctagtaac agccatatg ctatcgtgt cagcatgag 
      tttcataaa cagtgcatt actaactgg ctgaaacga 
      caaagtgcc tgtccggtg tgtagagtg cacgccgtt 
      attgatgag gagtttcca ccactgaag tcttaattt 
      ttgtttgag ttgtgttat ttctatagt ttattgttc 
      ttatgtttt gccgctttt atgatgtta acacaatga 
      tatattcta ttaattagc ccaaaatta agtagttat 
      tattattat tcagtattt tcaataaag tattttcaa 
      tatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY