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Gene information
General information
TC008240 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7127975..7143986
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008240
      iBeetle screen iB_04449

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
      Days post injection



      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008240 RA
        MQSSLILLQ DYCEASQRP LVLKFRVQY PVTNKLILI 
        TCIDEGWTR RSEPVKGRN LSTRKKLEE EKKRKQAEI 
        ERKRAEVRA RMEEASKAK KAKKGFMTP ERKKKLRLL 
        LRKKAAEEL KKEQERKAA ERRRIIEER CGKPKLIDD 
        ANEAELQTI CQSYHQRIA KLEGDKYDL EIIEKQKAR 
        QLKKICKEY YDRMYICEE QKWDLEREV RKRDWEISD 
        LNSQVNDLR GKFVKPTLK KVSKYENKF AKLQKKAAE 
        FNFRNQLKV VKKKEFTLE EEDKEKKPD WSKKGEEKK 
        ENGEEGEAP PAEGQPPAE GGPAPEGAP PSEGAPPAE 
        GAPALEGAP PAEGAPPAE GAPPAEGAP PAEGAPPAE 
        GAPPAEGAP PAEGAPPAE GAPPAEGEA PKPEEPTGG 
        FETFKQLVI HENYDIIAL PETVLAPTE NKEVVSIPN 
        YSLVENRKN SGDGVVLYI KNSLKYKVV DLTADNPEE 
        PQLNFDQLL DKLKQSAAA APATEGETP AAPAGEAPA 
        VPAGEAPAA PAAEAPAAP VEGAPAEAP PAAATAETP 
        AVPAETPPA EAPPAEAAP AAEAAPAET APAEPAAET 
        ETEVPPVEA SATPIIEAA A
      • >TC008240 RB
        MQSSLILLQ DYCEASQRP LVLKFRVQY PVTNKLILI 
        TCIDEGWTR RSEPVKGRN LSTRKKLEE EKKRKQAEI 
        ERKRAEVRA RMEEASKAK KAKKGFMTP ERKKKLRLL 
        LRKKAAEEL KKEQERKAA ERRRIIEER CGKPKLIDD 
        ANEDELQSI CKQYYTKIQ ELEGRKYDL EYIVRMKDI 
        ESTHLYILG SLKGICQQY HKRIGELEN QKYDLEKEV 
        EFRDFQVEW ALRRACQLK KICKEYYDR MYICEEQKW 
        DLEREVRKR DWEISDLNS QVNDLRGKF VKPTLKKVS 
        KYENKFAKL QKKAAEFNF RNQLKVVKK KEFTLEEED 
        KEKKPDWSK KGEEKKENG EEGEAPPAE GQPPAEGGP 
        APEGAPPSE GAPPAEGAP ALEGAPPAE GAPPAEGAP 
        PAEGAPPAE GAPPAEGAP PAEGAPPAE GAPPAEGAP 
        PAEGEAPKP EEPTGGFET FKQLVIHEN YDIIALPET 
        VLAPTENKE VVSIPNYSL VENRKNSGD GVVLYIKNS 
        LKYKVVDLT ADNPEEPQL NFDQLLDKL KQSAAAAPA 
        TEGETPAAP AGEAPAVPA GEAPAAPAA EAPAAPVEG 
        APAEAPPAA ATAETPAVP AETPPAEAP PAEAAPAAE 
        AAPAETAPA EPAAETETE VPPVEASAT PIIEAAA
      CDS sequence
      • >TC008240 RA
        atgcagtct agccttata cttttacag gattactgc 
        gaagcctct caacggccg ctagtgctg aagttccgt 
        gtgcaatat ccagttaca aacaaatta atattaatt 
        acttgcatc gacgaaggt tggacgcga agaagcgaa 
        cccgtcaaa ggcagaaat ctctcaacg agaaagaaa 
        ctggaggag gaaaagaag aggaagcag gcggagatc 
        gagcgcaag agggccgaa gtcagggcc cgcatggaa 
        gaggcctcg aaagccaag aaggccaag aaaggtttc 
        atgacccct gagagaaag aagaaactt aggttgctg 
        ttaagaaag aaagccgct gaggaattg aagaaagag 
        caagaacgc aaagccgcc gaaagaaga cgcatcatt 
        gaggagcgt tgcggtaaa cccaagctc atcgacgat 
        gccaatgaa gctgaactt caaactatt tgtcaaagc 
        tatcaccaa agaattgcg aaacttgaa ggggataaa 
        tatgacttg gaaataatc gaaaagcaa aaagcccgc 
        cagctgaag aagatttgt aaagagtat tatgatcga 
        atgtacatt tgcgaggaa cagaagtgg gatttggaa 
        cgcgaagtc cgaaaaaga gattgggag atctccgac 
        ctcaacagt caagtcaac gaccttagg ggcaaattc 
        gtcaaacca accttgaag aaagtctcc aaatacgag 
        aacaaattc gccaagtta caaaagaag gccgctgag 
        tttaacttc aggaatcaa ctcaaagtt gtaaagaag 
        aaggagttc acccttgaa gaagaagac aaagagaag 
        aaaccagat tggtccaag aagggagag gaaaagaag 
        gaaaacggg gaagaaggc gaagccccg cctgcagaa 
        ggtcaacct cctgccgaa ggcggtcct gcccctgaa 
        ggagcacct ccttcagaa ggtgctccg cccgccgaa 
        ggcgcccct gcacttgaa ggagcccca ccggctgaa 
        ggcgcccca cccgctgaa ggggcccct cccgctgag 
        ggtgctcca ccagccgaa ggggcccca cctgctgaa 
        ggtgcacca cctgcagaa ggcgctccg cccgcagaa 
        ggtgcccca cccgccgaa ggtgcccca ccggcagaa 
        ggcgaagcc cccaaaccg gaagagccc accggcgga 
        ttcgagaca tttaaacaa cttgtaata cacgaaaat 
        tacgatatt attgcttta cccgaaaca gtcctagcc 
        cccactgaa aacaaagaa gtagtttct atccccaat 
        tattcttta gtagagaac cgtaagaat agtggcgac 
        ggcgtggtt ttgtacata aaaaattcg cttaaatat 
        aaagttgtt gatttgaca gccgataat ccggaagag 
        cctcagttg aatttcgat caacttttg gacaaactg 
        aaacagagt gctgcagca gctccggcg acagaaggc 
        gaaacacca gcagctcct gctggagag gctcctgcc 
        gttcctgcc ggagaggct ccagccgct cctgctgcg 
        gaagctccg gccgctcct gttgaggga gctccagcg 
        gaagcgccc cctgcagct gcaacagcg gaaacacca 
        gcagtgccg gcggaaaca cctcctgct gaagcacca 
        ccagctgaa gctgcgccg gctgctgaa gccgctcct 
        gctgaaact gcccctgct gaacctgcc gctgaaact 
        gaaactgag gtgccgcca gttgaagca tcagctact 
        cctatcatt gaagctgcc gcttaa
      • >TC008240 RB
        atgcagtct agccttata cttttacag gattactgc 
        gaagcctct caacggccg ctagtgctg aagttccgt 
        gtgcaatat ccagttaca aacaaatta atattaatt 
        acttgcatc gacgaaggt tggacgcga agaagcgaa 
        cccgtcaaa ggcagaaat ctctcaacg agaaagaaa 
        ctggaggag gaaaagaag aggaagcag gcggagatc 
        gagcgcaag agggccgaa gtcagggcc cgcatggaa 
        gaggcctcg aaagccaag aaggccaag aaaggtttc 
        atgacccct gagagaaag aagaaactt aggttgctg 
        ttaagaaag aaagccgct gaggaattg aagaaagag 
        caagaacgc aaagccgcc gaaagaaga cgcatcatt 
        gaggagcgt tgcggtaaa cccaagctc atcgacgat 
        gccaatgaa gatgagttg caaagtatc tgcaaacaa 
        tattacacc aaaattcaa gaattggag ggacgcaag 
        tatgatttg gaatatatc gttagaatg aaagatatc 
        gagtccaca cacttgtat attctagga agccttaag 
        ggtatttgc caacaatat cataaacgt ataggagaa 
        cttgaaaat cagaaatat gatcttgaa aaggaggtg 
        gaattcaga gattttcag gtagaatgg gcacttagg 
        agggcatgc cagctgaag aagatttgt aaagagtat 
        tatgatcga atgtacatt tgcgaggaa cagaagtgg 
        gatttggaa cgcgaagtc cgaaaaaga gattgggag 
        atctccgac ctcaacagt caagtcaac gaccttagg 
        ggcaaattc gtcaaacca accttgaag aaagtctcc 
        aaatacgag aacaaattc gccaagtta caaaagaag 
        gccgctgag tttaacttc aggaatcaa ctcaaagtt 
        gtaaagaag aaggagttc acccttgaa gaagaagac 
        aaagagaag aaaccagat tggtccaag aagggagag 
        gaaaagaag gaaaacggg gaagaaggc gaagccccg 
        cctgcagaa ggtcaacct cctgccgaa ggcggtcct 
        gcccctgaa ggagcacct ccttcagaa ggtgctccg 
        cccgccgaa ggcgcccct gcacttgaa ggagcccca 
        ccggctgaa ggcgcccca cccgctgaa ggggcccct 
        cccgctgag ggtgctcca ccagccgaa ggggcccca 
        cctgctgaa ggtgcacca cctgcagaa ggcgctccg 
        cccgcagaa ggtgcccca cccgccgaa ggtgcccca 
        ccggcagaa ggcgaagcc cccaaaccg gaagagccc 
        accggcgga ttcgagaca tttaaacaa cttgtaata 
        cacgaaaat tacgatatt attgcttta cccgaaaca 
        gtcctagcc cccactgaa aacaaagaa gtagtttct 
        atccccaat tattcttta gtagagaac cgtaagaat 
        agtggcgac ggcgtggtt ttgtacata aaaaattcg 
        cttaaatat aaagttgtt gatttgaca gccgataat 
        ccggaagag cctcagttg aatttcgat caacttttg 
        gacaaactg aaacagagt gctgcagca gctccggcg 
        acagaaggc gaaacacca gcagctcct gctggagag 
        gctcctgcc gttcctgcc ggagaggct ccagccgct 
        cctgctgcg gaagctccg gccgctcct gttgaggga 
        gctccagcg gaagcgccc cctgcagct gcaacagcg 
        gaaacacca gcagtgccg gcggaaaca cctcctgct 
        gaagcacca ccagctgaa gctgcgccg gctgctgaa 
        gccgctcct gctgaaact gcccctgct gaacctgcc 
        gctgaaact gaaactgag gtgccgcca gttgaagca 
        tcagctact cctatcatt gaagctgcc gcttaa
      mRNA sequence
      • >TC008240 RA
        tccagcggc cggtaaagc caatatcgt aattatagt 
        tacttatca gtattcaat accgagtgg tggcgtttt 
        tcctgcatt tttccctcc gaggaaaac caaaaccgc 
        ctcagctcc aactgtggt cactttccc agcgctgga 
        cgccaatca aatcaaaat aattcgtct ataaataaa 
        tcaaaccgg aaaaaacgg ggggaagta tccctcgac 
        ttccgttgt atgaagccg caaaaatgc agtctagcc 
        ttatacttt tacaggatt actgcgaag cctctcaac 
        ggccgctag tgctgaagt tccgtgtgc aatatccag 
        ttacaaaca aattaatat taattactt gcatcgacg 
        aaggttgga cgcgaagaa gcgaacccg tcaaaggca 
        gaaatctct caacgagaa agaaactgg aggaggaaa 
        agaagagga agcaggcgg agatcgagc gcaagaggg 
        ccgaagtca gggcccgca tggaagagg cctcgaaag 
        ccaagaagg ccaagaaag gtttcatga cccctgaga 
        gaaagaaga aacttaggt tgctgttaa gaaagaaag 
        ccgctgagg aattgaaga aagagcaag aacgcaaag 
        ccgccgaaa gaagacgca tcattgagg agcgttgcg 
        gtaaaccca agctcatcg acgatgcca atgaagctg 
        aacttcaaa ctatttgtc aaagctatc accaaagaa 
        ttgcgaaac ttgaagggg ataaatatg acttggaaa 
        taatcgaaa agcaaaaag cccgccagc tgaagaaga 
        tttgtaaag agtattatg atcgaatgt acatttgcg 
        aggaacaga agtgggatt tggaacgcg aagtccgaa 
        aaagagatt gggagatct ccgacctca acagtcaag 
        tcaacgacc ttaggggca aattcgtca aaccaacct 
        tgaagaaag tctccaaat acgagaaca aattcgcca 
        agttacaaa agaaggccg ctgagttta acttcagga 
        atcaactca aagttgtaa agaagaagg agttcaccc 
        ttgaagaag aagacaaag agaagaaac cagattggt 
        ccaagaagg gagaggaaa agaaggaaa acggggaag 
        aaggcgaag ccccgcctg cagaaggtc aacctcctg 
        ccgaaggcg gtcctgccc ctgaaggag cacctcctt 
        cagaaggtg ctccgcccg ccgaaggcg cccctgcac 
        ttgaaggag ccccaccgg ctgaaggcg ccccacccg 
        ctgaagggg cccctcccg ctgagggtg ctccaccag 
        ccgaagggg ccccacctg ctgaaggtg caccacctg 
        cagaaggcg ctccgcccg cagaaggtg ccccacccg 
        ccgaaggtg ccccaccgg cagaaggcg aagccccca 
        aaccggaag agcccaccg gcggattcg agacattta 
        aacaacttg taatacacg aaaattacg atattattg 
        ctttacccg aaacagtcc tagccccca ctgaaaaca 
        aagaagtag tttctatcc ccaattatt ctttagtag 
        agaaccgta agaatagtg gcgacggcg tggttttgt 
        acataaaaa attcgctta aatataaag ttgttgatt 
        tgacagccg ataatccgg aagagcctc agttgaatt 
        tcgatcaac ttttggaca aactgaaac agagtgctg 
        cagcagctc cggcgacag aaggcgaaa caccagcag 
        ctcctgctg gagaggctc ctgccgttc ctgccggag 
        aggctccag ccgctcctg ctgcggaag ctccggccg 
        ctcctgttg agggagctc cagcggaag cgccccctg 
        cagctgcaa cagcggaaa caccagcag tgccggcgg 
        aaacacctc ctgctgaag caccaccag ctgaagctg 
        cgccggctg ctgaagccg ctcctgctg aaactgccc 
        ctgctgaac ctgccgctg aaactgaaa ctgaggtgc 
        cgccagttg aagcatcag ctactccta tcattgaag 
        ctgccgctt aaaaatgaa gccatatct gtgccgcca 
        cgctgctat tgttccact ttgttttat tattgaact 
        gtttgattt attttattg tagtttttg agaaatata 
        ttatttttt agactttgt ccatttgtt ttattgacg 
        gaagtgtat ccgaaaatc actcccatg gtcataaac 
        ccattgttg cggttcgtc ctacccatc aactagtca 
        cgtgatgca agagcacgc gtatgttag aaatttaat 
        ccgttcctc cgtcagtgc gcaaccgac aagctgtag 
        tgagatatt ttgtgttgt gtggtttga aagaaacat 
        cgtgggttg aaaaattaa cataaattt cttatttgg 
        ataaaactg tggcaatgt aaactttct acagtcttg 
        gaaacacaa aaaaagtaa gtcaaatat tttttcttt 
        tttttttaa ataaatcat tcaacaatt a
      • >TC008240 RB
        tccagcggc cggtaaagc caatatcgt aattatagt 
        tacttatca gtattcaat accgagtgg tggcgtttt 
        tcctgcatt tttccctcc gaggaaaac caaaaccgc 
        ctcagctcc aactgtggt cactttccc agcgctgga 
        cgccaatca aatcaaaat aattcgtct ataaataaa 
        tcaaaccgg aaaaaacgg ggggaagta tccctcgac 
        ttccgttgt atgaagccg caaaaatgc agtctagcc 
        ttatacttt tacaggatt actgcgaag cctctcaac 
        ggccgctag tgctgaagt tccgtgtgc aatatccag 
        ttacaaaca aattaatat taattactt gcatcgacg 
        aaggttgga cgcgaagaa gcgaacccg tcaaaggca 
        gaaatctct caacgagaa agaaactgg aggaggaaa 
        agaagagga agcaggcgg agatcgagc gcaagaggg 
        ccgaagtca gggcccgca tggaagagg cctcgaaag 
        ccaagaagg ccaagaaag gtttcatga cccctgaga 
        gaaagaaga aacttaggt tgctgttaa gaaagaaag 
        ccgctgagg aattgaaga aagagcaag aacgcaaag 
        ccgccgaaa gaagacgca tcattgagg agcgttgcg 
        gtaaaccca agctcatcg acgatgcca atgaagatg 
        agttgcaaa gtatctgca aacaatatt acaccaaaa 
        ttcaagaat tggagggac gcaagtatg atttggaat 
        atatcgtta gaatgaaag atatcgagt ccacacact 
        tgtatattc taggaagcc ttaagggta tttgccaac 
        aatatcata aacgtatag gagaacttg aaaatcaga 
        aatatgatc ttgaaaagg aggtggaat tcagagatt 
        ttcaggtag aatgggcac ttaggaggg catgccagc 
        tgaagaaga tttgtaaag agtattatg atcgaatgt 
        acatttgcg aggaacaga agtgggatt tggaacgcg 
        aagtccgaa aaagagatt gggagatct ccgacctca 
        acagtcaag tcaacgacc ttaggggca aattcgtca 
        aaccaacct tgaagaaag tctccaaat acgagaaca 
        aattcgcca agttacaaa agaaggccg ctgagttta 
        acttcagga atcaactca aagttgtaa agaagaagg 
        agttcaccc ttgaagaag aagacaaag agaagaaac 
        cagattggt ccaagaagg gagaggaaa agaaggaaa 
        acggggaag aaggcgaag ccccgcctg cagaaggtc 
        aacctcctg ccgaaggcg gtcctgccc ctgaaggag 
        cacctcctt cagaaggtg ctccgcccg ccgaaggcg 
        cccctgcac ttgaaggag ccccaccgg ctgaaggcg 
        ccccacccg ctgaagggg cccctcccg ctgagggtg 
        ctccaccag ccgaagggg ccccacctg ctgaaggtg 
        caccacctg cagaaggcg ctccgcccg cagaaggtg 
        ccccacccg ccgaaggtg ccccaccgg cagaaggcg 
        aagccccca aaccggaag agcccaccg gcggattcg 
        agacattta aacaacttg taatacacg aaaattacg 
        atattattg ctttacccg aaacagtcc tagccccca 
        ctgaaaaca aagaagtag tttctatcc ccaattatt 
        ctttagtag agaaccgta agaatagtg gcgacggcg 
        tggttttgt acataaaaa attcgctta aatataaag 
        ttgttgatt tgacagccg ataatccgg aagagcctc 
        agttgaatt tcgatcaac ttttggaca aactgaaac 
        agagtgctg cagcagctc cggcgacag aaggcgaaa 
        caccagcag ctcctgctg gagaggctc ctgccgttc 
        ctgccggag aggctccag ccgctcctg ctgcggaag 
        ctccggccg ctcctgttg agggagctc cagcggaag 
        cgccccctg cagctgcaa cagcggaaa caccagcag 
        tgccggcgg aaacacctc ctgctgaag caccaccag 
        ctgaagctg cgccggctg ctgaagccg ctcctgctg 
        aaactgccc ctgctgaac ctgccgctg aaactgaaa 
        ctgaggtgc cgccagttg aagcatcag ctactccta 
        tcattgaag ctgccgctt aaaaatgaa gccatatct 
        gtgccgcca cgctgctat tgttccact ttgttttat 
        tattgaact gtttgattt attttattg tagtttttg 
        agaaatata ttatttttt agactttgt ccatttgtt 
        ttattgacg gaagtgtat ccgaaaatc actcccatg 
        gtcataaac ccattgttg cggttcgtc ctacccatc 
        aactagtca cgtgatgca agagcacgc gtatgttag 
        aaatttaat ccgttcctc cgtcagtgc gcaaccgac 
        aagctgtag tgagatatt ttgtgttgt gtggtttga 
        aagaaacat cgtgggttg aaaaattaa cataaattt 
        cttatttgg ataaaactg tggcaatgt aaactttct 
        acagtcttg gaaacacaa aaaaagtaa gtcaaatat 
        tttttcttt tttttttaa ataaatcat tcaacaatt 
        a
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