Status
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Gene information
General information
TC008231 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7081545..7084110
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008231
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04448

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008231 RA
        MKFMLSLLA LGYVAIAIA APSLPVHPG GIGGLFKGP 
        NSETIIKGP DGSVITAAA EGGEVHTDL EPIVVAEEV 
        VPVEPVVPV EAVAVPAPP VENKQSSDL IGPSGRIVT 
        QGSQSIIAG PASTTITGP AVLAETVVK GAPVVGTPI 
        IGAAPVVAP IAVTAAPIA VTASPLPLV PETDLVAPA 
        VVATGISSV TATSPNAVA TASVSPHLS IASVSASHG 
        AAATASLAP NIATTSVET ASPLPIISS TAAPVLMSS 
        GAPVAPGIH GVTVNGFNN HLVHEVSTA LPLPVVPST 
        EVVSSTVSP IVSSTVSPV IQKGIAVKG LVGSTDHIH 
        LGPQLVYDS PTPSPLPVI HSTPTPVIP DVTNRIHQQ 
        GLAAHGLVG TNDRVDLGP RLIHDQLAI DNRQLHLNP 
        ALIPSANII PPEISVTPK TPIARLRPT PGTLLTTPG 
        LGTVANSPN ILREEGSVG INVTPDSLH YVPQSQHIL 
        REYGRAGLG VTPNTLQYG PQSPHLIRE GGGIGLGVT 
        PNTLQYGAQ SPHILREEG NVGLGVTPN TLEYGPQSP 
        HLIREEGGV RLGVTPNTL QYGAQPSHI LREEEGAAL 
        GVTPNTLQY GPQSRDDGR AVQYIPQPQ LRNQEQPQL 
        SPQQLAALQ YQQNLINNQ ILYASGGQS TGPALFTPT 
        EGPEEEDRP GEDPNVAIG GARITPPPV IPQQGVLGS 
        ELAKSGVDA SQVQLTNVP LSDVSNLPE DSNVKRAVL 
        YNVRAQSRG RHGRQVEEE KKKH
      CDS sequence
      • >TC008231 RA
        atgaagttt atgctttca ctactagcc ttgggctac 
        gtagcgata gctatcgcc gcccccagt ctgccggtg 
        catcctgga ggcatcggg gggttgttt aaaggaccc 
        aattcagaa accatcatc aaagggcca gacggaagc 
        gttataact gcagctgcc gaaggtgga gaagtccac 
        actgacctt gaacctatc gtggttgca gaagaagta 
        gtacctgtt gaacccgtg gtacctgtg gaagcagta 
        gcggtacct gccccacct gtggaaaac aaacaaagc 
        tcggactta attggcccc tctggcaga attgttacg 
        caagggtcg cagtccatt atagccggg ccagcttca 
        accacaatt actggtcct gccgtatta gcagaaacc 
        gtagttaaa ggagcgcca gttgttgga acaccaatt 
        ataggtgca gctcccgtt gtagctcca atagcagtt 
        acggcagct cctattgct gtaacagca tcaccatta 
        cccctggtc cctgaaact gatttggta gctccggct 
        gttgtagca actggaatt tctagtgta actgcaact 
        tctccgaat gctgttgcc acagcttca gtttcacca 
        catttgtca attgcgtca gtttcagca agccatgga 
        gctgcagca acagcgtct ctagctcca aatatagct 
        acaacatct gttgaaaca gcatctcct ttacctatc 
        atttcgtca acagctgca ccagtttta atgtcaagt 
        ggagctcct gttgctcca ggaatacac ggtgtaaca 
        gttaatggt tttaataat catttggtt catgaagtt 
        tcaacagca ttaccttta ccggtggtt ccttcaaca 
        gaagttgtt tcgtcaaca gtatcacca atagtttcg 
        tcaactgtt tctccagtt atacaaaaa gggatagct 
        gttaaaggt cttgttgga agtactgat catatccat 
        ctaggacct caattggtt tacgactct ccaacacca 
        tcaccttta ccagtgatt cattctaca ccaactcca 
        gtaattccc gatgtgacc aacagaata caccagcaa 
        ggtcttgca gctcatggt cttgtagga actaatgac 
        cgtgttgat cttggacct cgcttaatc catgatcag 
        ttagctatt gacaacaga cagttgcac ctcaatcca 
        gctttaatc ccatctgct aatataatc cctcctgaa 
        atatcagtg actccgaaa acgcccatt gcccgctta 
        aggccaact cctggtact ttgctcaca actccagga 
        ctgggaaca gtcgcaaac tccccaaat attttaagg 
        gaggaaggg agtgttggt ataaatgta actccggat 
        agtttacat tacgtacca caatcccag cacatttta 
        agggaatat ggaagggca ggattgggc gtaactcca 
        aacacttta caatacgga cctcaatct cctcacttg 
        ataagagaa ggaggaggt ataggatta ggcgtaact 
        cccaatact ttacaatat ggagctcag tctccacac 
        attttacga gaagaagga aatgtaggt ttaggtgtg 
        accccaaac actttagaa tacggacct caatcccca 
        cacttaata agagaagaa ggaggtgta agattgggc 
        gtaactccc aatacttta caatatgga gctcagcct 
        tctcatata ttaagagaa gaagaaggt gcagcatta 
        ggtgtaact cccaacact ttacaatat ggacctcag 
        tcaagagat gacggaagg gccgtacaa tacattcct 
        caacctcaa ttaagaaat caagaacaa cctcaatta 
        agcccccaa caacttgcg gctttacaa taccaacaa 
        aatttgatc aataatcag attctgtat gcatcagga 
        ggtcagtct acaggccca gctttattt actccaact 
        gaaggccca gaagaagaa gacagacca ggtgaagat 
        ccaaacgtt gcaatagga ggtgctcga ataactcca 
        ccgccggtt attccacaa cagggagtt ttgggttca 
        gaattggca aaatctggg gttgatgcg agtcaggtg 
        cagttgacc aatgtgcct ttgtcggat gtgtctaat 
        ttgccagag gattctaat gttaaaagg gctgtgctt 
        tataatgtg agagcgcaa agtagaggt cggcatgga 
        agacaagtt gaagaggaa aagaagaaa cattga
      mRNA sequence
      • >TC008231 RA
        tcagatcct agttacagt tcacagatt ctgcaatga 
        agtttatgc tttcactac tagccttgg gctacgtag 
        cgatagcta tcgccgccc ccagtctgc cggtgcatc 
        ctggaggca tcggggggt tgtttaaag gacccaatt 
        cagaaacca tcatcaaag ggccagacg gaagcgtta 
        taactgcag ctgccgaag gtggagaag tccacactg 
        accttgaac ctatcgtgg ttgcagaag aagtagtac 
        ctgttgaac ccgtggtac ctgtggaag cagtagcgg 
        tacctgccc cacctgtgg aaaacaaac aaagctcgg 
        acttaattg gcccctctg gcagaattg ttacgcaag 
        ggtcgcagt ccattatag ccgggccag cttcaacca 
        caattactg gtcctgccg tattagcag aaaccgtag 
        ttaaaggag cgccagttg ttggaacac caattatag 
        gtgcagctc ccgttgtag ctccaatag cagttacgg 
        cagctccta ttgctgtaa cagcatcac cattacccc 
        tggtccctg aaactgatt tggtagctc cggctgttg 
        tagcaactg gaatttcta gtgtaactg caacttctc 
        cgaatgctg ttgccacag cttcagttt caccacatt 
        tgtcaattg cgtcagttt cagcaagcc atggagctg 
        cagcaacag cgtctctag ctccaaata tagctacaa 
        catctgttg aaacagcat ctcctttac ctatcattt 
        cgtcaacag ctgcaccag ttttaatgt caagtggag 
        ctcctgttg ctccaggaa tacacggtg taacagtta 
        atggtttta ataatcatt tggttcatg aagtttcaa 
        cagcattac ctttaccgg tggttcctt caacagaag 
        ttgtttcgt caacagtat caccaatag tttcgtcaa 
        ctgtttctc cagttatac aaaaaggga tagctgtta 
        aaggtcttg ttggaagta ctgatcata tccatctag 
        gacctcaat tggtttacg actctccaa caccatcac 
        ctttaccag tgattcatt ctacaccaa ctccagtaa 
        ttcccgatg tgaccaaca gaatacacc agcaaggtc 
        ttgcagctc atggtcttg taggaacta atgaccgtg 
        ttgatcttg gacctcgct taatccatg atcagttag 
        ctattgaca acagacagt tgcacctca atccagctt 
        taatcccat ctgctaata taatccctc ctgaaatat 
        cagtgactc cgaaaacgc ccattgccc gcttaaggc 
        caactcctg gtactttgc tcacaactc caggactgg 
        gaacagtcg caaactccc caaatattt taagggagg 
        aagggagtg ttggtataa atgtaactc cggatagtt 
        tacattacg taccacaat cccagcaca ttttaaggg 
        aatatggaa gggcaggat tgggcgtaa ctccaaaca 
        ctttacaat acggacctc aatctcctc acttgataa 
        gagaaggag gaggtatag gattaggcg taactccca 
        atactttac aatatggag ctcagtctc cacacattt 
        tacgagaag aaggaaatg taggtttag gtgtgaccc 
        caaacactt tagaatacg gacctcaat ccccacact 
        taataagag aagaaggag gtgtaagat tgggcgtaa 
        ctcccaata ctttacaat atggagctc agccttctc 
        atatattaa gagaagaag aaggtgcag cattaggtg 
        taactccca acactttac aatatggac ctcagtcaa 
        gagatgacg gaagggccg tacaataca ttcctcaac 
        ctcaattaa gaaatcaag aacaacctc aattaagcc 
        cccaacaac ttgcggctt tacaatacc aacaaaatt 
        tgatcaata atcagattc tgtatgcat caggaggtc 
        agtctacag gcccagctt tatttactc caactgaag 
        gcccagaag aagaagaca gaccaggtg aagatccaa 
        acgttgcaa taggaggtg ctcgaataa ctccaccgc 
        cggttattc cacaacagg gagttttgg gttcagaat 
        tggcaaaat ctggggttg atgcgagtc aggtgcagt 
        tgaccaatg tgcctttgt cggatgtgt ctaatttgc 
        cagaggatt ctaatgtta aaagggctg tgctttata 
        atgtgagag cgcaaagta gaggtcggc atggaagac 
        aagttgaag aggaaaaga agaaacatt gaaagacgg 
        gggaaaagc aactagtca gtattgagt tgtggtgta 
        atgttatat ttgcttaaa taagtttgc taatctttg 
        tattatttt atatagtat ttataaagt tgtaataaa 
        taatcgttt tgtgt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY