Status
Please wait ...
Gene information
General information
TC032855
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008224,TC008225
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6970870..6991154
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032855
    iBeetle screen iB_04447

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032855 RA
      MGKVEQLND DVPGLSPQT EASKLLEQA LMQMDGIIS 
      GNTSSLTAH SPDYGFAAS PPGVREAAN SLVTAIQNV 
      PAPPPPDPN VAKVLLHWL QRPDEHSTR VRIVKIATC 
      GISHASGAA MGDSSGGGD EVAFVLRLK PAPTPPVRR 
      RRPHEEKHT VPKGWDVVI EREPPPVPP RVQPSAPPP 
      ELLTWHPKP YYVYPDYRN FYAERQSFV RNSGGGDLV 
      LPPSGVSLV SGGKPPRPL PRMSAKGED AEMSRQSSL 
      SSRTWWLLS RVLSADKEV SMRELCRAG GTVVAAVVA 
      VLEKQQQAL SAPPPSKNS LSASTPDDN FQSAVKDFD 
      SVAEDVERI VVAQRKCQE DDSSSEDDS ESSGEHVLE 
      GLETIAEED RNTSSALSY KGLDEDSSP EDDSTLKLT 
      NTWQNDSTS DSSSSSSSL PRSSTRCDD DFQWIRKEM 
      KMEEVAAKP PIGRSRTPR RDKPAPGSL DRRRHQRVE 
      RDQKSRSTH VPKSPPRLD DFAPCQNWC CSENYAFEN 
      FHRRHERMD PQRYGSSPM LMDRGSHPD LFGCRHEMM 
      GCCGYHVAP PCCFYGERG YHWTPAPFQ KSSDQQDEK 
      FRKLQYEKD NLQLQVQVL TEQIEAQTD KIADLEKLL 
      MERKQHLSE TEEKLQREV LSRSSLETQ KLEMMSIVS 
      ELKLQQAAL ERENLELRS QFNNNGDIK KPPLGPRMV 
      QTSTPVHHS SNQALRMSP SPSPVTLTG SSPRRDDIP 
      APKTPPANY KRQIDLHYG SLPRQQFIS NGSSISVVD 
      SNANPKKGV AFGRGLSSL IGGRAKGFS VPNLAETEK 
      IIEGGSGES PQSPTLQFN RNKGIKKIF GRMKRSGSG 
      NLEELPGIG EFQRGGVRA TAAARLGWS EPQLTVKPD 
      KPFAEWDTE NICDWLQDL SLDQYIPEA KRWVKSGQQ 
      LQEASITDI EKELNIKNP LHKKKLQLA LIDTQENGS 
      SDPYLTKAG QLDMAWVLR WLDDTGLPQ HKENFLINR 
      VDGRVLHRL TIDDLALLH VTSLLHVAS IKRGIQVLR 
      ENNYEPGCL QRRSSPEDP PQPTPKIIS LWTTHRVME 
      WLKAVDLAE YAPNLRGAG VHGGLMVYE TKFTAELLA 
      TLLSIPPGK TLLRRHLKT HFNELLGKE IIQEKREAE 
      STMGYIPLT PSSKLKVAK KSQFSLKRK KSKGEADYG 
      DLVCPLNSD KQSGELNGS APNMGMMKT ESLRVEDVS 
      HHVEQKLVR SSPASIANS PVPQRHV
    CDS sequence
    • >TC032855 RA
      atgggtaaa gtcgagcaa ttaaacgac gacgtcccg 
      ggcctgtcc ccccagacc gaggccagc aagctcctg 
      gagcaagcc cttatgcaa atggatggc atcatctca 
      ggcaacact agttcactt acggcccat tcgcccgat 
      tatgggttt gcggcgtcg ccgccgggg gtccgagaa 
      gccgccaac tcgctcgtc acggccata cagaacgtc 
      cccgcgccg ccgcccccg gaccccaac gtcgccaaa 
      gtgctccta cactggctt cagcgtccc gacgagcac 
      tcaacacgt gttcgaatc gtaaaaatc gccacatgt 
      ggaattagt cacgcttcg ggcgcggcg atgggtgat 
      tcctccgga ggcggcgac gaagtcgcc ttcgtgcta 
      cgtctcaaa ccggcgccg acgcctccg gtgcgacgc 
      cgccgtccc cacgaggag aagcacacc gtgccgaaa 
      ggctgggac gtggtgatc gaacgcgaa ccgccaccg 
      gtgccgccg agagttcag ccgtcggcg ccgcccccg 
      gaactccta acgtggcat cccaaacct tattatgtc 
      tacccggat taccggaat ttctacgcg gaaagacag 
      agtttcgtg cggaattcc ggcggcggt gatttagtt 
      ttgccgccg tcgggagtg tcacttgtt agtggtggg 
      aaaccgccg cgaccgctg ccgaggatg agcgcgaag 
      ggggaggat gcagaaatg tcgaggcaa agcagtctc 
      tcgtcgcgg acgtggtgg ctgttatct cgcgtctta 
      agtgccgat aaggaggtg tcgatgcgc gagctgtgc 
      cgggcgggc ggcaccgtc gtggcggcc gtcgtcgcc 
      gtcctcgag aagcagcag caggcgctg agcgcccca 
      cctccgtcc aaaaatagc ctcagcgcc agcacgccc 
      gacgataat tttcaatcg gcggtgaag gatttcgac 
      agcgtggcc gaagacgtc gagaggatc gtggtggcg 
      cagaggaag tgccaggag gacgactcg tccagcgag 
      gatgactcg gagtcgagc ggggagcac gtgctggag 
      ggactggag accatcgcc gaggaggac aggaatacg 
      tcgagtgcg ttgagttac aaaggtctc gacgaagac 
      tccagtccc gaagacgat tccacgcta aagctcacc 
      aacacgtgg caaaatgac tcgacatcg gactcctcc 
      tcgtcctct tcgtcgctc cccaggagt tccacgcgc 
      tgtgacgac gacttccag tggatacgc aaggaaatg 
      aagatggag gaggtggcg gcgaagccg cccatcggc 
      cgttccagg accccccgg agggacaaa cccgccccc 
      gggagtctg gaccggcgg cggcatcag cgggtggaa 
      agggaccag aagtccagg tccacccac gtgcccaag 
      agccccccc agactcgac gacttcgcc ccgtgccag 
      aactggtgt tgcagcgaa aattacgca tttgaaaac 
      ttccacagg aggcacgag aggatggac ccgcagagg 
      tacgggagt agtcccatg ttgatggac agggggagt 
      catcccgat ttgttcggg tgcagacat gagatgatg 
      gggtgttgt gggtatcac gtggcaccg ccgtgttgt 
      ttttacggg gagcggggg tatcattgg acgccggcg 
      ccgttccag aagtcgagc gaccaacag gacgagaaa 
      ttccgaaaa ttacaatat gaaaaggac aatctccag 
      ctacaagtg caagtcctc accgagcag atcgaagca 
      cagaccgat aaaatcgcc gatttggag aaactgttg 
      atggaaagg aagcaacac ttgtccgaa accgaagag 
      aaattgcag cgtgaggtc ttatcgcga tcgtcgctc 
      gagacacaa aaactcgaa atgatgtcg atcgtcagc 
      gaactcaaa ttgcaacag gcggccctc gagcgcgaa 
      aacctcgaa ctgaggagt cagttcaac aacaacgga 
      gacatcaaa aagccccct ttagggccc cgcatggtc 
      cagacgtcg actccggtc caccattcc agcaatcaa 
      gctttgagg atgtcgccg tcgccgagt ccggtaact 
      ctgactggg agcagcccc cggagggac gacattccg 
      gcccccaaa actccacct gcgaactac aaacgccag 
      atcgatttg cattacggt agtctgcct cgtcagcaa 
      ttcatcagt aatggaagc agcatttcg gtggtcgat 
      tccaatgcg aatccgaaa aagggtgtg gcgttcggt 
      aggggactt tcttcactc attgggggg cgggccaag 
      ggcttctcc gtgcccaat ctagctgaa actgaaaaa 
      attatcgag ggcgggtca ggtgagtca ccgcaatca 
      ccgacgttg caattcaac cggaacaaa ggtattaaa 
      aaaattttc ggacgcatg aaacgctcc ggctccgga 
      aatttggaa gagttgcct ggaattgga gaatttcaa 
      cgcggagga gtccgggcg acggccgcc gcacgcctc 
      ggctggagc gagccgcaa ctaacagta aagccggac 
      aagcctttc gccgagtgg gacaccgag aacatctgc 
      gattggctg caagacctc agcctagac cagtacatc 
      cccgaagcg aagcgctgg gtgaaatcc ggccagcaa 
      ttacaggaa gcttcaatt acggacatc gaaaaagaa 
      ctgaacatc aaaaatccc ctccacaag aaaaaactc 
      caactggcc ctgatcgat acccaagag aacggttca 
      agtgaccct tacttaacg aaagcgggt caattggac 
      atggcctgg gtgttgcga tggttagac gacacgggt 
      ttgccccaa cacaaggaa aatttcctc ataaatcgc 
      gtcgatgga cgagttttg caccgattg acaatcgac 
      gatttggct ttgttgcac gttacttcc ctgctccac 
      gttgcgagt atcaagcga gggattcaa gttttgaga 
      gagaataac tacgaaccg gggtgtcta caacggaga 
      tcgtcaccg gaagatccg ccacaacca acccccaaa 
      ataatatcg ctctggacg actcatcgc gttatggag 
      tggcttaaa gctgttgat ttggctgag tacgctccg 
      aatttacga ggtgcgggg gttcatggc gggttgatg 
      gtgtacgag accaagttc acggcggaa ctgttggcc 
      acattgctt tcaattccg ccggggaaa accctactg 
      aggcgccac ctcaaaacg catttcaac gaattgttg 
      ggcaaggaa ataatccaa gagaaaaga gaggctgag 
      tcaaccatg gggtatata ccgctgacg ccgtcgtcg 
      aaattaaaa gtcgccaag aagtcgcag ttttccctg 
      aagcggaag aagagcaag ggcgaagcg gactacggc 
      gacctggtg tgccctctg aattccgac aaacagtca 
      ggtgaactg aacggttct gctcctaac atgggtatg 
      atgaagacg gaaagccta agggtcgag gatgtctct 
      catcacgtt gagcaaaaa ctggtgcgg tcctcgccg 
      gcatcgatt gctaattca ccggtacca caaagacat 
      gtttaa
    mRNA sequence
    • >TC032855 RA
      ctgttgtga cgtaacttt tctgtcaac gacgtaaac 
      aaagccaaa tgttgagtt tataagccc cgtaaacag 
      tgtgtgttt atttctttt caagtagat tagtgttaa 
      tagatataa acctctttt tttgcgcac ccgtaatta 
      actttgcgc gcgaatttt gccaaatgt gtgccctaa 
      cctcaacgt aaactttcc cattaggtg tcaattaaa 
      ctaattgct aaaatgggt aaagtcgag caattaaac 
      gacgacgtc ccgggcctg tccccccag accgaggcc 
      agcaagctc ctggagcaa gcccttatg caaatggat 
      ggcatcatc tcaggcaac actagttca cttacggcc 
      cattcgccc gattatggg tttgcggcg tcgccgccg 
      ggggtccga gaagccgcc aactcgctc gtcacggcc 
      atacagaac gtccccgcg ccgccgccc ccggacccc 
      aacgtcgcc aaagtgctc ctacactgg cttcagcgt 
      cccgacgag cactcaaca cgtgttcga atcgtaaaa 
      atcgccaca tgtggaatt agtcacgct tcgggcgcg 
      gcgatgggt gattcctcc ggaggcggc gacgaagtc 
      gccttcgtg ctacgtctc aaaccggcg ccgacgcct 
      ccggtgcga cgccgccgt ccccacgag gagaagcac 
      accgtgccg aaaggctgg gacgtggtg atcgaacgc 
      gaaccgcca ccggtgccg ccgagagtt cagccgtcg 
      gcgccgccc ccggaactc ctaacgtgg catcccaaa 
      ccttattat gtctacccg gattaccgg aatttctac 
      gcggaaaga cagagtttc gtgcggaat tccggcggc 
      ggtgattta gttttgccg ccgtcggga gtgtcactt 
      gttagtggt gggaaaccg ccgcgaccg ctgccgagg 
      atgagcgcg aagggggag gatgcagaa atgtcgagg 
      caaagcagt ctctcgtcg cggacgtgg tggctgtta 
      tctcgcgtc ttaagtgcc gataaggag gtgtcgatg 
      cgcgagctg tgccgggcg ggcggcacc gtcgtggcg 
      gccgtcgtc gccgtcctc gagaagcag cagcaggcg 
      ctgagcgcc ccacctccg tccaaaaat agcctcagc 
      gccagcacg cccgacgat aattttcaa tcggcggtg 
      aaggatttc gacagcgtg gccgaagac gtcgagagg 
      atcgtggtg gcgcagagg aagtgccag gaggacgac 
      tcgtccagc gaggatgac tcggagtcg agcggggag 
      cacgtgctg gagggactg gagaccatc gccgaggag 
      gacaggaat acgtcgagt gcgttgagt tacaaaggt 
      ctcgacgaa gactccagt cccgaagac gattccacg 
      ctaaagctc accaacacg tggcaaaat gactcgaca 
      tcggactcc tcctcgtcc tcttcgtcg ctccccagg 
      agttccacg cgctgtgac gacgacttc cagtggata 
      cgcaaggaa atgaagatg gaggaggtg gcggcgaag 
      ccgcccatc ggccgttcc aggaccccc cggagggac 
      aaacccgcc cccgggagt ctggaccgg cggcggcat 
      cagcgggtg gaaagggac cagaagtcc aggtccacc 
      cacgtgccc aagagcccc cccagactc gacgacttc 
      gccccgtgc cagaactgg tgttgcagc gaaaattac 
      gcatttgaa aacttccac aggaggcac gagaggatg 
      gacccgcag aggtacggg agtagtccc atgttgatg 
      gacaggggg agtcatccc gatttgttc gggtgcaga 
      catgagatg atggggtgt tgtgggtat cacgtggca 
      ccgccgtgt tgtttttac ggggagcgg gggtatcat 
      tggacgccg gcgccgttc cagaagtcg agcgaccaa 
      caggacgag aaattccga aaattacaa tatgaaaag 
      gacaatctc cagctacaa gtgcaagtc ctcaccgag 
      cagatcgaa gcacagacc gataaaatc gccgatttg 
      gagaaactg ttgatggaa aggaagcaa cacttgtcc 
      gaaaccgaa gagaaattg cagcgtgag gtcttatcg 
      cgatcgtcg ctcgagaca caaaaactc gaaatgatg 
      tcgatcgtc agcgaactc aaattgcaa caggcggcc 
      ctcgagcgc gaaaacctc gaactgagg agtcagttc 
      aacaacaac ggagacatc aaaaagccc cctttaggg 
      ccccgcatg gtccagacg tcgactccg gtccaccat 
      tccagcaat caagctttg aggatgtcg ccgtcgccg 
      agtccggta actctgact gggagcagc ccccggagg 
      gacgacatt ccggccccc aaaactcca cctgcgaac 
      tacaaacgc cagatcgat ttgcattac ggtagtctg 
      cctcgtcag caattcatc agtaatgga agcagcatt 
      tcggtggtc gattccaat gcgaatccg aaaaagggt 
      gtggcgttc ggtagggga ctttcttca ctcattggg 
      gggcgggcc aagggcttc tccgtgccc aatctagct 
      gaaactgaa aaaattatc gagggcggg tcaggtgag 
      tcaccgcaa tcaccgacg ttgcaattc aaccggaac 
      aaaggtatt aaaaaaatt ttcggacgc atgaaacgc 
      tccggctcc ggaaatttg gaagagttg cctggaatt 
      ggagaattt caacgcgga ggagtccgg gcgacggcc 
      gccgcacgc ctcggctgg agcgagccg caactaaca 
      gtaaagccg gacaagcct ttcgccgag tgggacacc 
      gagaacatc tgcgattgg ctgcaagac ctcagccta 
      gaccagtac atccccgaa gcgaagcgc tgggtgaaa 
      tccggccag caattacag gaagcttca attacggac 
      atcgaaaaa gaactgaac atcaaaaat cccctccac 
      aagaaaaaa ctccaactg gccctgatc gatacccaa 
      gagaacggt tcaagtgac ccttactta acgaaagcg 
      ggtcaattg gacatggcc tgggtgttg cgatggtta 
      gacgacacg ggtttgccc caacacaag gaaaatttc 
      ctcataaat cgcgtcgat ggacgagtt ttgcaccga 
      ttgacaatc gacgatttg gctttgttg cacgttact 
      tccctgctc cacgttgcg agtatcaag cgagggatt 
      caagttttg agagagaat aactacgaa ccggggtgt 
      ctacaacgg agatcgtca ccggaagat ccgccacaa 
      ccaaccccc aaaataata tcgctctgg acgactcat 
      cgcgttatg gagtggctt aaagctgtt gatttggct 
      gagtacgct ccgaattta cgaggtgcg ggggttcat 
      ggcgggttg atggtgtac gagaccaag ttcacggcg 
      gaactgttg gccacattg ctttcaatt ccgccgggg 
      aaaacccta ctgaggcgc cacctcaaa acgcatttc 
      aacgaattg ttgggcaag gaaataatc caagagaaa 
      agagaggct gagtcaacc atggggtat ataccgctg 
      acgccgtcg tcgaaatta aaagtcgcc aagaagtcg 
      cagttttcc ctgaagcgg aagaagagc aagggcgaa 
      gcggactac ggcgacctg gtgtgccct ctgaattcc 
      gacaaacag tcaggtgaa ctgaacggt tctgctcct 
      aacatgggt atgatgaag acggaaagc ctaagggtc 
      gaggatgtc tctcatcac gttgagcaa aaactggtg 
      cggtcctcg ccggcatcg attgctaat tcaccggta 
      ccacaaaga catgtttaa taagttttt tagtatatt 
      gattgataa aaggaaagt atttatcaa atatgtctt 
      cccccccaa acaatttgc cttaggaaa gtgcatgag 
      attcctatt gtgtttgtt tatcggcgg cgaaattat 
      aataatcgt gtcaaatgt gccaaaccg attattttt 
      ttctgtttt cgtggaatt tctattttt ttgtattaa 
      cagtattta aatatcgtt gttaccaat tgagtagag 
      atagatttt agacgtatt taacaaact gaccaaaca 
      attcgtatt aagcgtagg ttatgaaat tgttgacat 
      ttttttgta tcgtcaccg tcacacgca tcactccca 
      ttgtatttt atgtaattg aatattttt attatcgca 
      ttaaacttg taaacattt c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY