Status
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Gene information
General information
TC032853
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008219,TC008218
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6901040..6906548
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032853
    iBeetle screen iB_04446

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032853 RA
      MNFKTVFVV FLFMKLHGC NIVLPMIND LIEHFNKTQ 
      IILAYLCDK NDAFQVVSH FSKKLYQVN VLSPNSRDM 
      PYPTPPAFL TYVLDAGCS NTKQLLLLA SEQKQFATP 
      FKWIVYYNN PVELSFFID EYFTKTNIL VDSDVTLAT 
      INPTSGTFD LNKIYKRKI NGSIIIENI GIWGRGLGV 
      TDTGYEKIT YKRRRNLTK TVLKSCIVI TNNDSLNHL 
      TDKRDIHID SIAKVNYVL VQHLSDTIN ASLEYSVRG 
      TWGYKDNKS QWSGMIGEL TRNEADIGG TALFLTSDR 
      IRVIDYIAM TTPTRSKFI FRQPKLSYV ANVFTLPFD 
      ASVWASVCG LLVIIAGLL YVVVRWEWK KKDYVQVVN 
      ESNITEIHN SWLDVVFIT FGALCQQGS SSVPFSIPG 
      RITLIFLLV SLMFLYTSY SANIVALLQ SSSSSIQTL 
      QDILNSRLD VGVDNTVFN HFYFPNATE PIRRAIYQQ 
      KVAPPGQKP KFYPIEEGI RKMRQGLFA FHVETGPGY 
      KFVSEIFRE DEKCGLQEI QYLQVPDPW LAIQKNSSY 
      KKMLKVGLR LLQENGIQE REVGLIYTK KPQCLARGS 
      SFISVGLVD CYPAAVVLA GGIGAALAV LILEIYVHQ 
      RIHEKIHTL ATYFDSNIA FLKLTAFWI YDDETTRRK 
      KYLQHAYNI FWIFYLFVA YQPAELLYV YYSFNDLSV 
      FLRALRDIG NHVSLAYKA FNYFIMRRD ILKLMETLQ 
      HGNYHYEDC GDFQPKLIV DEEKKEALK WTKYFLNFC 
      NAICLSMFA NGVFTFIFL SDKQYVERN GQRVYHQEQ 
      PVNTVSPFG SGTKLRFFV TFIYTMIAL TFYAWTIVA 
      LDSLFITIM SCISSHLKI LQGAFKTVR ARCIMRTRA 
      ERLLKEETL HDPPFLENC VNKEMIRCI KHLQTVLSV 
      SGKLESIYS TQTFVQTFI SLGEMCFSL YLLSETADQ 
      NIGNEITYL IATGFELLM YCWFGNRIT EASLKISYA 
      LYESDWFPT SLSFKKQII FTMTRMQKP INVTIGKIT 
      PLAFSTFLT IARGAYSFF TFLKQRHGI NH
    CDS sequence
    • >TC032853 RA
      atgaatttc aaaacagtt ttcgtagtt ttcctcttc 
      atgaaactt cacggttgt aatattgtg ttaccaatg 
      attaatgat ttaatcgaa cattttaac aaaacgcaa 
      attattttg gcctatctg tgtgacaaa aacgacgcc 
      tttcaggta gtgagccac ttttcgaaa aaattgtac 
      caagtcaat gttttatcc ccaaattct cgtgacatg 
      ccctatcca acaccacca gcttttctc acttatgtt 
      ttggatgct ggttgttcg aatacaaaa caattacta 
      cttttggcc agtgaacaa aaacaattc gctacgcct 
      ttcaaatgg atcgtttat tacaacaat ccagtcgaa 
      ttatcgttt tttatcgac gaatatttc accaaaacg 
      aacattttg gtcgatagt gatgtcacc ctcgctaca 
      ataaacccc acaagcggc acatttgac ttgaacaaa 
      atctacaaa aggaaaatt aacggttcc ataataatt 
      gaaaatatc ggaatttgg ggacgcggc ctcggtgtg 
      actgacacc ggctacgaa aaaatcacg tacaaacgc 
      cggcgaaat ttgacgaaa acggtcctg aagtcatgc 
      attgtgatc acaaataac gactctttg aaccacttg 
      actgacaaa agggacatt cacatcgac agcatcgct 
      aaagttaat tacgtccta gtccaacat ctgtccgat 
      acgataaac gcgtctttg gaatattcg gtgaggggc 
      acttggggc tataaagac aacaagtcc caatggagc 
      ggaatgatt ggcgagttg acaagaaac gaggcagat 
      atcgggggc acagcccta tttcttaca agcgatcgg 
      atcagagtc atagattac atcgcaatg accaccccg 
      acacggtct aaatttatt ttccgacaa ccgaagttg 
      tcatacgtt gccaatgtt tttactcta ccgtttgac 
      gcctccgtg tgggcttct gtgtgcggc ctgcttgtc 
      attattgcc ggtctgttg tatgttgta gtgagatgg 
      gagtggaaa aagaaggat tatgtccaa gttgtgaac 
      gaatcgaac atcacggaa attcacaat tcttggctc 
      gacgttgtt tttatcaca tttggggct ttgtgtcaa 
      caaggctct tcgtcggta cctttcagt attcccggt 
      cggatcact ttaattttc ctcctggtg tctctcatg 
      ttcttgtac acatcttac tctgcaaac attgtagcg 
      ctgctgcaa tcgtcttca agcagtatt caaacactt 
      caagacatc ctcaattcg agattagac gtgggtgtt 
      gataacacg gtcttcaat catttctat ttccccaac 
      gcaactgag ccaatccgc cgagccatc taccaacaa 
      aaggtcgct ccacctggc cagaagccc aaattttac 
      cccatcgag gaaggcatt cgcaaaatg aggcaaggt 
      ctgttcgcg tttcacgtc gagacaggc ccggggtac 
      aaattcgtt agcgagatt ttccgagag gatgaaaaa 
      tgtggcttg caagaaatc cagtatttg caagtgcct 
      gacccttgg ttggcgatt cagaaaaac tcctcgtat 
      aaaaaaatg ttgaaagtg ggacttcgt ttgcttcaa 
      gaaaatggc attcaggag cgggaagtt gggctaatt 
      tatacgaaa aaaccgcag tgtttggca agagggagc 
      agttttatc agtgtgggg ttggttgat tgttacccg 
      gctgctgtt gtcttagcg ggcgggatt ggggcggct 
      ttggcggtg ctcatcctc gagatatat gttcatcaa 
      aggattcat gaaaaaatc cacactctc gcaacgtat 
      ttcgattca aacattgcg ttcctaaag ttgacggct 
      ttctggatt tatgacgac gaaaccaca agacgcaag 
      aaatacttg caacatgcc tataacatt ttttggatc 
      ttttacttg tttgttgct taccaaccg gcagaactg 
      ctttatgtc tattacagt ttcaatgac ttgagcgtt 
      tttctgcga gctttgcgc gatatcggc aaccacgtc 
      tcgctggcg tacaaagct ttcaattac ttcatcatg 
      cggagagat attttgaag ttgatggaa acgctccag 
      cacggcaat taccattac gaggattgc ggggatttt 
      cagccaaaa ctgattgtg gacgaggag aagaaggag 
      gctttgaag tggacgaaa tactttcta aacttttgc 
      aacgcgatt tgtttgagc atgtttgcg aacggcgta 
      ttcacgttc attttcctg agtgacaaa caatacgtt 
      gaaaggaac ggtcagagg gtttaccac caggaacag 
      ccagttaac acagtctcg ccgttcggt tccgggact 
      aaattacgg tttttcgtc acttttatc tacacaatg 
      atcgcgtta acgttttac gcgtggacg attgttgcc 
      ctcgactcg ctttttatc acgataatg agctgcatc 
      agtagccac ctcaaaata ctacaaggg gcgttcaaa 
      actgttcga gcccgttgt attatgcgg acgcgagcc 
      gaaagactc ttgaaagag gagacttta catgacccc 
      ccttttttg gaaaattgc gtcaacaag gaaatgatt 
      cgttgcata aaacaccta caaaccgtc ctcagtgtt 
      tcagggaag cttgagtca atttacagc acgcaaacg 
      ttcgtccag acgttcatt tctttgggc gaaatgtgc 
      ttctcgctg tacctccta agcgaaaca gcggatcaa 
      aacatagga aatgaaata acttatctt attgctacc 
      ggctttgaa ctcctgatg tactgctgg ttcggaaat 
      agaatcaca gaggcgagt ctcaaaata tcttacgcg 
      ctgtatgag agtgactgg tttccgaca agtctctcg 
      ttcaagaaa caaattatc ttcacgatg acaaggatg 
      caaaagccc atcaatgtc accattggc aaaattaca 
      cccttggct tttagcact tttcttact atcgcacga 
      ggggcatac tcgtttttc acgtttctg aagcaaaga 
      cacggcata aatcactga
    mRNA sequence
    • >TC032853 RA
      gtattgaaa caacttcat aaaatgaat ttcaaaaca 
      gttttcgta gttttcctc ttcatgaaa cttcacggt 
      tgtaatatt gtgttacca atgattaat gatttaatc 
      gaacatttt aacaaaacg caaattatt ttggcctat 
      ctgtgtgac aaaaacgac gcctttcag gtagtgagc 
      cacttttcg aaaaaattg taccaagtc aatgtttta 
      tccccaaat tctcgtgac atgccctat ccaacacca 
      ccagctttt ctcacttat gttttggat gctggttgt 
      tcgaataca aaacaatta ctacttttg gccagtgaa 
      caaaaacaa ttcgctacg cctttcaaa tggatcgtt 
      tattacaac aatccagtc gaattatcg ttttttatc 
      gacgaatat ttcaccaaa acgaacatt ttggtcgat 
      agtgatgtc accctcgct acaataaac cccacaagc 
      ggcacattt gacttgaac aaaatctac aaaaggaaa 
      attaacggt tccataata attgaaaat atcggaatt 
      tggggacgc ggcctcggt gtgactgac accggctac 
      gaaaaaatc acgtacaaa cgccggcga aatttgacg 
      aaaacggtc ctgaagtca tgcattgtg atcacaaat 
      aacgactct ttgaaccac ttgactgac aaaagggac 
      attcacatc gacagcatc gctaaagtt aattacgtc 
      ctagtccaa catctgtcc gatacgata aacgcgtct 
      ttggaatat tcggtgagg ggcacttgg ggctataaa 
      gacaacaag tcccaatgg agcggaatg attggcgag 
      ttgacaaga aacgaggca gatatcggg ggcacagcc 
      ctatttctt acaagcgat cggatcaga gtcatagat 
      tacatcgca atgaccacc ccgacacgg tctaaattt 
      attttccga caaccgaag ttgtcatac gttgccaat 
      gtttttact ctaccgttt gacgcctcc gtgtgggct 
      tctgtgtgc ggcctgctt gtcattatt gccggtctg 
      ttgtatgtt gtagtgaga tgggagtgg aaaaagaag 
      gattatgtc caagttgtg aacgaatcg aacatcacg 
      gaaattcac aattcttgg ctcgacgtt gtttttatc 
      acatttggg gctttgtgt caacaaggc tcttcgtcg 
      gtacctttc agtattccc ggtcggatc actttaatt 
      ttcctcctg gtgtctctc atgttcttg tacacatct 
      tactctgca aacattgta gcgctgctg caatcgtct 
      tcaagcagt attcaaaca cttcaagac atcctcaat 
      tcgagatta gacgtgggt gttgataac acggtcttc 
      aatcatttc tatttcccc aacgcaact gagccaatc 
      cgccgagcc atctaccaa caaaaggtc gctccacct 
      ggccagaag cccaaattt taccccatc gaggaaggc 
      attcgcaaa atgaggcaa ggtctgttc gcgtttcac 
      gtcgagaca ggcccgggg tacaaattc gttagcgag 
      attttccga gaggatgaa aaatgtggc ttgcaagaa 
      atccagtat ttgcaagtg cctgaccct tggttggcg 
      attcagaaa aactcctcg tataaaaaa atgttgaaa 
      gtgggactt cgtttgctt caagaaaat ggcattcag 
      gagcgggaa gttgggcta atttatacg aaaaaaccg 
      cagtgtttg gcaagaggg agcagtttt atcagtgtg 
      gggttggtt gattgttac ccggctgct gttgtctta 
      gcgggcggg attggggcg gctttggcg gtgctcatc 
      ctcgagata tatgttcat caaaggatt catgaaaaa 
      atccacact ctcgcaacg tatttcgat tcaaacatt 
      gcgttccta aagttgacg gctttctgg atttatgac 
      gacgaaacc acaagacgc aagaaatac ttgcaacat 
      gcctataac attttttgg atcttttac ttgtttgtt 
      gcttaccaa ccggcagaa ctgctttat gtctattac 
      agtttcaat gacttgagc gtttttctg cgagctttg 
      cgcgatatc ggcaaccac gtctcgctg gcgtacaaa 
      gctttcaat tacttcatc atgcggaga gatattttg 
      aagttgatg gaaacgctc cagcacggc aattaccat 
      tacgaggat tgcggggat tttcagcca aaactgatt 
      gtggacgag gagaagaag gaggctttg aagtggacg 
      aaatacttt ctaaacttt tgcaacgcg atttgtttg 
      agcatgttt gcgaacggc gtattcacg ttcattttc 
      ctgagtgac aaacaatac gttgaaagg aacggtcag 
      agggtttac caccaggaa cagccagtt aacacagtc 
      tcgccgttc ggttccggg actaaatta cggtttttc 
      gtcactttt atctacaca atgatcgcg ttaacgttt 
      tacgcgtgg acgattgtt gccctcgac tcgcttttt 
      atcacgata atgagctgc atcagtagc cacctcaaa 
      atactacaa ggggcgttc aaaactgtt cgagcccgt 
      tgtattatg cggacgcga gccgaaaga ctcttgaaa 
      gaggagact ttacatgac ccccctttt ttggaaaat 
      tgcgtcaac aaggaaatg attcgttgc ataaaacac 
      ctacaaacc gtcctcagt gtttcaggg aagcttgag 
      tcaatttac agcacgcaa acgttcgtc cagacgttc 
      atttctttg ggcgaaatg tgcttctcg ctgtacctc 
      ctaagcgaa acagcggat caaaacata ggaaatgaa 
      ataacttat cttattgct accggcttt gaactcctg 
      atgtactgc tggttcgga aatagaatc acagaggcg 
      agtctcaaa atatcttac gcgctgtat gagagtgac 
      tggtttccg acaagtctc tcgttcaag aaacaaatt 
      atcttcacg atgacaagg atgcaaaag cccatcaat 
      gtcaccatt ggcaaaatt acacccttg gcttttagc 
      acttttctt actatcgca cgaggggca tactcgttt 
      ttcacgttt ctgaagcaa agacacggc ataaatcac 
      tgaataaat aaagaaaaa aaataaaaa ttattaaac 
      acg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY