| General information | |||
| TC008204 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 6607386..6617829 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008204 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04443 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC008204 RA
MSVDSVSTP STPSTPCSP PAEPFSIEV ITAGDADEV LRFLRCFFI RDEPLNHSI GLLDGKETC PELESYSIK DLDSGLNLK AVSNGKIIG VCLNGILTR GYLEEIEEA NCEDKKFES ILRLLDHVA VQSDIFSHF PDVDKAMVV KILSVDSSL RGRGIAKDL MNRTRDLAR ELGCGIMTA DCTSHFTAR ALKKLGFEC IYSLNYEDY KVNGEVVFT PETPHAAVT VYTQRIVK
- >TC008204 RB
MAVQMQPSF ILTEKFEKL KFTDFVSVD SVSTPSTPS TPCSPPAEP FSIEVITAG DADEVLRFL RCFFIRDEP LNHSIGLLD GKETCPELE SYSIKDLDS GLNLKAVSN GKIIGVCLN GILTRGYLE EIEEANCED KKFESILRL LDHVAVQSD IFSHFPDVD KAMVVKILS VDSSLRGRG IAKDLMNRT RDLARELGC GIMTADCTS HFTARALKK LGFECIYSL NYEDYKVNG EVVFTPETP HAAVTVYTQ RIVK
- >TC008204 RA
atgtccgtc gattcagtc tccaccccc tcgacacct tccaccccc tgcagcccc ccagccgag cccttcagc atcgaagtg atcacagcg ggggacgcg gacgaagtc ttgcggttc ttgcggtgt tttttcatc cgagatgag cccctgaac cacagtatt gggcttctg gacggcaag gaaacctgt ccggagctg gagtcgtat tccatcaag gatttagac agtggactt aatctcaag gcggtttcc aacgggaag ataatcggg gtttgcctc aacggcatc ctgactcgg ggctatctg gaggagatc gaagaagcg aactgtgag gacaaaaag ttcgagtcg attttgagg ctcctcgac catgtcgca gtccagtcg gacattttc tcgcatttc ccggacgtg gataaagcc atggttgtg aaaatcctc tctgtggat agctctcta cgaggacga gggatcgca aaggatctg atgaatagg acgagagac cttgcccga gaactgggc tgcggtata atgaccgcc gattgcacg agtcacttc acagcaagg gctctgaag aagctggga tttgaatgc atttactct ctcaattac gaagactac aaagttaat ggagaagtc gtgtttacg ccggagaca ccccatgcc gccgtcact gtttacacg caaagaatt gtgaaataa
- >TC008204 RB
atggcggtt caaatgcag cctagtttc atcctcacc gagaagttc gaaaagctc aaattcacc gattttgtg tccgtcgat tcagtctcc accccctcg acaccttcc accccctgc agcccccca gccgagccc ttcagcatc gaagtgatc acagcgggg gacgcggac gaagtcttg cggttcttg cggtgtttt ttcatccga gatgagccc ctgaaccac agtattggg cttctggac ggcaaggaa acctgtccg gagctggag tcgtattcc atcaaggat ttagacagt ggacttaat ctcaaggcg gtttccaac gggaagata atcggggtt tgcctcaac ggcatcctg actcggggc tatctggag gagatcgaa gaagcgaac tgtgaggac aaaaagttc gagtcgatt ttgaggctc ctcgaccat gtcgcagtc cagtcggac attttctcg catttcccg gacgtggat aaagccatg gttgtgaaa atcctctct gtggatagc tctctacga ggacgaggg atcgcaaag gatctgatg aataggacg agagacctt gcccgagaa ctgggctgc ggtataatg accgccgat tgcacgagt cacttcaca gcaagggct ctgaagaag ctgggattt gaatgcatt tactctctc aattacgaa gactacaaa gttaatgga gaagtcgtg tttacgccg gagacaccc catgccgcc gtcactgtt tacacgcaa agaattgtg aaataa
- >TC008204 RA
aaccaagtg ttcatagct tcctcagtc gaattatat atctccggg gaaaacaaa gctccggag ccccgactg ttcaagtgg tgctccgga tgttcgaat cccagagtg aaaaatcga agcacttga cgaaaatac tgaacggcc tccaggttt gaaataaac caaatttcg cctcaagtg ttatgaatt tatatttag caaagtact tgagctgta ggaaaacaa caacaaaaa tgaatttaa taggcacgt ccctttata taacaggat gctgcacaa tgggatcat tttaaattc gtattgtaa ccctatatg tcgtaaata aacaaatac agttcaaat tgttgcttc tgtcggtga taacacgac acagtgctt gagtgataa gataaatac tttaaaaca ttttcgtct ttctgtcat ttcgtgcat tcatttcaa caaattgtg acggttttg caacaaata tgtccgtcg attcagtct ccaccccct cgacacctt ccaccccct gcagccccc cagccgagc ccttcagca tcgaagtga tcacagcgg gggacgcgg acgaagtct tgcggttct tgcggtgtt ttttcatcc gagatgagc ccctgaacc acagtattg ggcttctgg acggcaagg aaacctgtc cggagctgg agtcgtatt ccatcaagg atttagaca gtggactta atctcaagg cggtttcca acgggaaga taatcgggg tttgcctca acggcatcc tgactcggg gctatctgg aggagatcg aagaagcga actgtgagg acaaaaagt tcgagtcga ttttgaggc tcctcgacc atgtcgcag tccagtcgg acattttct cgcatttcc cggacgtgg ataaagcca tggttgtga aaatcctct ctgtggata gctctctac gaggacgag ggatcgcaa aggatctga tgaatagga cgagagacc ttgcccgag aactgggct gcggtataa tgaccgccg attgcacga gtcacttca cagcaaggg ctctgaaga agctgggat ttgaatgca tttactctc tcaattacg aagactaca aagttaatg gagaagtcg tgtttacgc cggagacac cccatgccg ccgtcactg tttacacgc aaagaattg tgaaataat cgtgccaca aggcggact tggtttcct gaaggcgca caatttgct tggtgtttg tgcttgtgg aacaacttt tagacacat tccctctac ttggaaatt tcaaaaata gattttaag acaattaat tatctaacc agtgtggat aattgttcc tcttgatgt attcgtttt ggaatacgt ggtttaggt tttattcat ttggaaaag tgcaatgtc tgtttaaat ttttatgtt tacatggag ttgctcatt gatgtatta taaaagttt ttaaacaaa tacaacgtc ttttattct
- >TC008204 RB
aaccaagtg ttcatagct tcctcagtc gaattatat atctccggg gaaaacaaa gctccggag ccccgactg ttcaagtgg tgctccgga tgttcgaat cccagagtg aaaaatcga agcacttga cgaaaatac tgaacggcc tccaggttt gaaataaac caaatttcg cctcaagtg ttatgaatt tatatttag caaagtact tgagctgta ggaaaacaa caacaaaaa tgaatttaa taggcacgt ccctttata taacaggat gctgcacaa tgggatcat tttaaattc gtattgtaa ccctatatg tcgtaaata aacaaatac agttcaaat tgttgcttc tgtcggtga taacacgac acagtgctt gagtgataa gataaatac tttaaaaca ttttcgtct ttctgtcat ttcgtgcat tcatttcaa caaattgtg acggttttg caacaaata tggccggcg agcaaataa ttgcatttg tctgttttc cttcaatcg gtcttaatc gcctcgcat ctcattata ataaatgag aaaatctcc acgtgaaaa tacaaaaaa taccgagcg tctcgagcg tgcgtactc gcagctgat tttcaactt gtagggttc ggggatata atataaaag tgcttgcga tgtaaaagc agaagtcgc tgaaacttt gccagtttc aatccgggt attttcgtg cggttgtaa ggattcagt gcttgtgtg tttgcaaca attaatcga aatggcggt tcaaatgca gcctagttt catcctcac cgagaagtt cgaaaagct caaattcac cgattttgt gtccgtcga ttcagtctc caccccctc gacaccttc caccccctg cagcccccc agccgagcc cttcagcat cgaagtgat cacagcggg ggacgcgga cgaagtctt gcggttctt gcggtgttt tttcatccg agatgagcc cctgaacca cagtattgg gcttctgga cggcaagga aacctgtcc ggagctgga gtcgtattc catcaagga tttagacag tggacttaa tctcaaggc ggtttccaa cgggaagat aatcggggt ttgcctcaa cggcatcct gactcgggg ctatctgga ggagatcga agaagcgaa ctgtgagga caaaaagtt cgagtcgat tttgaggct cctcgacca tgtcgcagt ccagtcgga cattttctc gcatttccc ggacgtgga taaagccat ggttgtgaa aatcctctc tgtggatag ctctctacg aggacgagg gatcgcaaa ggatctgat gaataggac gagagacct tgcccgaga actgggctg cggtataat gaccgccga ttgcacgag tcacttcac agcaagggc tctgaagaa gctgggatt tgaatgcat ttactctct caattacga agactacaa agttaatgg agaagtcgt gtttacgcc ggagacacc ccatgccgc cgtcactgt ttacacgca aagaattgt gaaataatc gtgccacaa ggcggactt ggtttcctg aaggcgcac aatttgctt ggtgtttgt gcttgtgga acaactttt agacacatt ccctctact tggaaattt caaaaatag attttaaga caattaatt atctaacca gtgtggata attgttcct cttgatgta ttcgttttg gaatacgtg gtttaggtt ttattcatt tggaaaagt gcaatgtct gtttaaatt tttatgttt acatggagt tgctcattg atgtattat aaaagtttt taaacaaat acaacgtct tttattct
