Status
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Gene information
General information
TC008204 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6607386..6617829
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008204
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008204 RA
      MSVDSVSTP STPSTPCSP PAEPFSIEV 
      ITAGDADEV LRFLRCFFI RDEPLNHSI 
      GLLDGKETC PELESYSIK DLDSGLNLK 
      AVSNGKIIG VCLNGILTR GYLEEIEEA 
      NCEDKKFES ILRLLDHVA VQSDIFSHF 
      PDVDKAMVV KILSVDSSL RGRGIAKDL 
      MNRTRDLAR ELGCGIMTA DCTSHFTAR 
      ALKKLGFEC IYSLNYEDY KVNGEVVFT 
      PETPHAAVT VYTQRIVK
    • >TC008204 RB
      MAVQMQPSF ILTEKFEKL KFTDFVSVD SVSTPSTPS 
      TPCSPPAEP FSIEVITAG DADEVLRFL RCFFIRDEP 
      LNHSIGLLD GKETCPELE SYSIKDLDS GLNLKAVSN 
      GKIIGVCLN GILTRGYLE EIEEANCED KKFESILRL 
      LDHVAVQSD IFSHFPDVD KAMVVKILS VDSSLRGRG 
      IAKDLMNRT RDLARELGC GIMTADCTS HFTARALKK 
      LGFECIYSL NYEDYKVNG EVVFTPETP HAAVTVYTQ 
      RIVK
    CDS sequence
    • >TC008204 RA
      atgtccgtc gattcagtc tccaccccc tcgacacct 
      tccaccccc tgcagcccc ccagccgag cccttcagc 
      atcgaagtg atcacagcg ggggacgcg gacgaagtc 
      ttgcggttc ttgcggtgt tttttcatc cgagatgag 
      cccctgaac cacagtatt gggcttctg gacggcaag 
      gaaacctgt ccggagctg gagtcgtat tccatcaag 
      gatttagac agtggactt aatctcaag gcggtttcc 
      aacgggaag ataatcggg gtttgcctc aacggcatc 
      ctgactcgg ggctatctg gaggagatc gaagaagcg 
      aactgtgag gacaaaaag ttcgagtcg attttgagg 
      ctcctcgac catgtcgca gtccagtcg gacattttc 
      tcgcatttc ccggacgtg gataaagcc atggttgtg 
      aaaatcctc tctgtggat agctctcta cgaggacga 
      gggatcgca aaggatctg atgaatagg acgagagac 
      cttgcccga gaactgggc tgcggtata atgaccgcc 
      gattgcacg agtcacttc acagcaagg gctctgaag 
      aagctggga tttgaatgc atttactct ctcaattac 
      gaagactac aaagttaat ggagaagtc gtgtttacg 
      ccggagaca ccccatgcc gccgtcact gtttacacg 
      caaagaatt gtgaaataa
    • >TC008204 RB
      atggcggtt caaatgcag cctagtttc atcctcacc 
      gagaagttc gaaaagctc aaattcacc gattttgtg 
      tccgtcgat tcagtctcc accccctcg acaccttcc 
      accccctgc agcccccca gccgagccc ttcagcatc 
      gaagtgatc acagcgggg gacgcggac gaagtcttg 
      cggttcttg cggtgtttt ttcatccga gatgagccc 
      ctgaaccac agtattggg cttctggac ggcaaggaa 
      acctgtccg gagctggag tcgtattcc atcaaggat 
      ttagacagt ggacttaat ctcaaggcg gtttccaac 
      gggaagata atcggggtt tgcctcaac ggcatcctg 
      actcggggc tatctggag gagatcgaa gaagcgaac 
      tgtgaggac aaaaagttc gagtcgatt ttgaggctc 
      ctcgaccat gtcgcagtc cagtcggac attttctcg 
      catttcccg gacgtggat aaagccatg gttgtgaaa 
      atcctctct gtggatagc tctctacga ggacgaggg 
      atcgcaaag gatctgatg aataggacg agagacctt 
      gcccgagaa ctgggctgc ggtataatg accgccgat 
      tgcacgagt cacttcaca gcaagggct ctgaagaag 
      ctgggattt gaatgcatt tactctctc aattacgaa 
      gactacaaa gttaatgga gaagtcgtg tttacgccg 
      gagacaccc catgccgcc gtcactgtt tacacgcaa 
      agaattgtg aaataa
    mRNA sequence
    • >TC008204 RA
      aaccaagtg ttcatagct tcctcagtc gaattatat 
      atctccggg gaaaacaaa gctccggag ccccgactg 
      ttcaagtgg tgctccgga tgttcgaat cccagagtg 
      aaaaatcga agcacttga cgaaaatac tgaacggcc 
      tccaggttt gaaataaac caaatttcg cctcaagtg 
      ttatgaatt tatatttag caaagtact tgagctgta 
      ggaaaacaa caacaaaaa tgaatttaa taggcacgt 
      ccctttata taacaggat gctgcacaa tgggatcat 
      tttaaattc gtattgtaa ccctatatg tcgtaaata 
      aacaaatac agttcaaat tgttgcttc tgtcggtga 
      taacacgac acagtgctt gagtgataa gataaatac 
      tttaaaaca ttttcgtct ttctgtcat ttcgtgcat 
      tcatttcaa caaattgtg acggttttg caacaaata 
      tgtccgtcg attcagtct ccaccccct cgacacctt 
      ccaccccct gcagccccc cagccgagc ccttcagca 
      tcgaagtga tcacagcgg gggacgcgg acgaagtct 
      tgcggttct tgcggtgtt ttttcatcc gagatgagc 
      ccctgaacc acagtattg ggcttctgg acggcaagg 
      aaacctgtc cggagctgg agtcgtatt ccatcaagg 
      atttagaca gtggactta atctcaagg cggtttcca 
      acgggaaga taatcgggg tttgcctca acggcatcc 
      tgactcggg gctatctgg aggagatcg aagaagcga 
      actgtgagg acaaaaagt tcgagtcga ttttgaggc 
      tcctcgacc atgtcgcag tccagtcgg acattttct 
      cgcatttcc cggacgtgg ataaagcca tggttgtga 
      aaatcctct ctgtggata gctctctac gaggacgag 
      ggatcgcaa aggatctga tgaatagga cgagagacc 
      ttgcccgag aactgggct gcggtataa tgaccgccg 
      attgcacga gtcacttca cagcaaggg ctctgaaga 
      agctgggat ttgaatgca tttactctc tcaattacg 
      aagactaca aagttaatg gagaagtcg tgtttacgc 
      cggagacac cccatgccg ccgtcactg tttacacgc 
      aaagaattg tgaaataat cgtgccaca aggcggact 
      tggtttcct gaaggcgca caatttgct tggtgtttg 
      tgcttgtgg aacaacttt tagacacat tccctctac 
      ttggaaatt tcaaaaata gattttaag acaattaat 
      tatctaacc agtgtggat aattgttcc tcttgatgt 
      attcgtttt ggaatacgt ggtttaggt tttattcat 
      ttggaaaag tgcaatgtc tgtttaaat ttttatgtt 
      tacatggag ttgctcatt gatgtatta taaaagttt 
      ttaaacaaa tacaacgtc ttttattct
    • >TC008204 RB
      aaccaagtg ttcatagct tcctcagtc gaattatat 
      atctccggg gaaaacaaa gctccggag ccccgactg 
      ttcaagtgg tgctccgga tgttcgaat cccagagtg 
      aaaaatcga agcacttga cgaaaatac tgaacggcc 
      tccaggttt gaaataaac caaatttcg cctcaagtg 
      ttatgaatt tatatttag caaagtact tgagctgta 
      ggaaaacaa caacaaaaa tgaatttaa taggcacgt 
      ccctttata taacaggat gctgcacaa tgggatcat 
      tttaaattc gtattgtaa ccctatatg tcgtaaata 
      aacaaatac agttcaaat tgttgcttc tgtcggtga 
      taacacgac acagtgctt gagtgataa gataaatac 
      tttaaaaca ttttcgtct ttctgtcat ttcgtgcat 
      tcatttcaa caaattgtg acggttttg caacaaata 
      tggccggcg agcaaataa ttgcatttg tctgttttc 
      cttcaatcg gtcttaatc gcctcgcat ctcattata 
      ataaatgag aaaatctcc acgtgaaaa tacaaaaaa 
      taccgagcg tctcgagcg tgcgtactc gcagctgat 
      tttcaactt gtagggttc ggggatata atataaaag 
      tgcttgcga tgtaaaagc agaagtcgc tgaaacttt 
      gccagtttc aatccgggt attttcgtg cggttgtaa 
      ggattcagt gcttgtgtg tttgcaaca attaatcga 
      aatggcggt tcaaatgca gcctagttt catcctcac 
      cgagaagtt cgaaaagct caaattcac cgattttgt 
      gtccgtcga ttcagtctc caccccctc gacaccttc 
      caccccctg cagcccccc agccgagcc cttcagcat 
      cgaagtgat cacagcggg ggacgcgga cgaagtctt 
      gcggttctt gcggtgttt tttcatccg agatgagcc 
      cctgaacca cagtattgg gcttctgga cggcaagga 
      aacctgtcc ggagctgga gtcgtattc catcaagga 
      tttagacag tggacttaa tctcaaggc ggtttccaa 
      cgggaagat aatcggggt ttgcctcaa cggcatcct 
      gactcgggg ctatctgga ggagatcga agaagcgaa 
      ctgtgagga caaaaagtt cgagtcgat tttgaggct 
      cctcgacca tgtcgcagt ccagtcgga cattttctc 
      gcatttccc ggacgtgga taaagccat ggttgtgaa 
      aatcctctc tgtggatag ctctctacg aggacgagg 
      gatcgcaaa ggatctgat gaataggac gagagacct 
      tgcccgaga actgggctg cggtataat gaccgccga 
      ttgcacgag tcacttcac agcaagggc tctgaagaa 
      gctgggatt tgaatgcat ttactctct caattacga 
      agactacaa agttaatgg agaagtcgt gtttacgcc 
      ggagacacc ccatgccgc cgtcactgt ttacacgca 
      aagaattgt gaaataatc gtgccacaa ggcggactt 
      ggtttcctg aaggcgcac aatttgctt ggtgtttgt 
      gcttgtgga acaactttt agacacatt ccctctact 
      tggaaattt caaaaatag attttaaga caattaatt 
      atctaacca gtgtggata attgttcct cttgatgta 
      ttcgttttg gaatacgtg gtttaggtt ttattcatt 
      tggaaaagt gcaatgtct gtttaaatt tttatgttt 
      acatggagt tgctcattg atgtattat aaaagtttt 
      taaacaaat acaacgtct tttattct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY