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Gene information
General information
TC008204 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6607386..6617829
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008204
    iBeetle screen iB_04443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008204 RA
      MSVDSVSTP STPSTPCSP PAEPFSIEV 
ITAGDADEV LRFLRCFFI RDEPLNHSI 
GLLDGKETC PELESYSIK DLDSGLNLK 
AVSNGKIIG VCLNGILTR GYLEEIEEA 
NCEDKKFES ILRLLDHVA VQSDIFSHF 
PDVDKAMVV KILSVDSSL RGRGIAKDL 
MNRTRDLAR ELGCGIMTA DCTSHFTAR 
ALKKLGFEC IYSLNYEDY KVNGEVVFT 
PETPHAAVT VYTQRIVK
    • >TC008204 RB
      MAVQMQPSF ILTEKFEKL KFTDFVSVD SVSTPSTPS 
TPCSPPAEP FSIEVITAG DADEVLRFL RCFFIRDEP 
LNHSIGLLD GKETCPELE SYSIKDLDS GLNLKAVSN 
GKIIGVCLN GILTRGYLE EIEEANCED KKFESILRL 
LDHVAVQSD IFSHFPDVD KAMVVKILS VDSSLRGRG 
IAKDLMNRT RDLARELGC GIMTADCTS HFTARALKK 
LGFECIYSL NYEDYKVNG EVVFTPETP HAAVTVYTQ 
RIVK
    CDS sequence
    • >TC008204 RA
      atgtccgtc gattcagtc tccaccccc tcgacacct 
tccaccccc tgcagcccc ccagccgag cccttcagc 
atcgaagtg atcacagcg ggggacgcg gacgaagtc 
ttgcggttc ttgcggtgt tttttcatc cgagatgag 
cccctgaac cacagtatt gggcttctg gacggcaag 
gaaacctgt ccggagctg gagtcgtat tccatcaag 
gatttagac agtggactt aatctcaag gcggtttcc 
aacgggaag ataatcggg gtttgcctc aacggcatc 
ctgactcgg ggctatctg gaggagatc gaagaagcg 
aactgtgag gacaaaaag ttcgagtcg attttgagg 
ctcctcgac catgtcgca gtccagtcg gacattttc 
tcgcatttc ccggacgtg gataaagcc atggttgtg 
aaaatcctc tctgtggat agctctcta cgaggacga 
gggatcgca aaggatctg atgaatagg acgagagac 
cttgcccga gaactgggc tgcggtata atgaccgcc 
gattgcacg agtcacttc acagcaagg gctctgaag 
aagctggga tttgaatgc atttactct ctcaattac 
gaagactac aaagttaat ggagaagtc gtgtttacg 
ccggagaca ccccatgcc gccgtcact gtttacacg 
caaagaatt gtgaaataa
    • >TC008204 RB
      atggcggtt caaatgcag cctagtttc atcctcacc 
gagaagttc gaaaagctc aaattcacc gattttgtg 
tccgtcgat tcagtctcc accccctcg acaccttcc 
accccctgc agcccccca gccgagccc ttcagcatc 
gaagtgatc acagcgggg gacgcggac gaagtcttg 
cggttcttg cggtgtttt ttcatccga gatgagccc 
ctgaaccac agtattggg cttctggac ggcaaggaa 
acctgtccg gagctggag tcgtattcc atcaaggat 
ttagacagt ggacttaat ctcaaggcg gtttccaac 
gggaagata atcggggtt tgcctcaac ggcatcctg 
actcggggc tatctggag gagatcgaa gaagcgaac 
tgtgaggac aaaaagttc gagtcgatt ttgaggctc 
ctcgaccat gtcgcagtc cagtcggac attttctcg 
catttcccg gacgtggat aaagccatg gttgtgaaa 
atcctctct gtggatagc tctctacga ggacgaggg 
atcgcaaag gatctgatg aataggacg agagacctt 
gcccgagaa ctgggctgc ggtataatg accgccgat 
tgcacgagt cacttcaca gcaagggct ctgaagaag 
ctgggattt gaatgcatt tactctctc aattacgaa 
gactacaaa gttaatgga gaagtcgtg tttacgccg 
gagacaccc catgccgcc gtcactgtt tacacgcaa 
agaattgtg aaataa
    mRNA sequence
    • >TC008204 RA
      aaccaagtg ttcatagct tcctcagtc gaattatat 
atctccggg gaaaacaaa gctccggag ccccgactg 
ttcaagtgg tgctccgga tgttcgaat cccagagtg 
aaaaatcga agcacttga cgaaaatac tgaacggcc 
tccaggttt gaaataaac caaatttcg cctcaagtg 
ttatgaatt tatatttag caaagtact tgagctgta 
ggaaaacaa caacaaaaa tgaatttaa taggcacgt 
ccctttata taacaggat gctgcacaa tgggatcat 
tttaaattc gtattgtaa ccctatatg tcgtaaata 
aacaaatac agttcaaat tgttgcttc tgtcggtga 
taacacgac acagtgctt gagtgataa gataaatac 
tttaaaaca ttttcgtct ttctgtcat ttcgtgcat 
tcatttcaa caaattgtg acggttttg caacaaata 
tgtccgtcg attcagtct ccaccccct cgacacctt 
ccaccccct gcagccccc cagccgagc ccttcagca 
tcgaagtga tcacagcgg gggacgcgg acgaagtct 
tgcggttct tgcggtgtt ttttcatcc gagatgagc 
ccctgaacc acagtattg ggcttctgg acggcaagg 
aaacctgtc cggagctgg agtcgtatt ccatcaagg 
atttagaca gtggactta atctcaagg cggtttcca 
acgggaaga taatcgggg tttgcctca acggcatcc 
tgactcggg gctatctgg aggagatcg aagaagcga 
actgtgagg acaaaaagt tcgagtcga ttttgaggc 
tcctcgacc atgtcgcag tccagtcgg acattttct 
cgcatttcc cggacgtgg ataaagcca tggttgtga 
aaatcctct ctgtggata gctctctac gaggacgag 
ggatcgcaa aggatctga tgaatagga cgagagacc 
ttgcccgag aactgggct gcggtataa tgaccgccg 
attgcacga gtcacttca cagcaaggg ctctgaaga 
agctgggat ttgaatgca tttactctc tcaattacg 
aagactaca aagttaatg gagaagtcg tgtttacgc 
cggagacac cccatgccg ccgtcactg tttacacgc 
aaagaattg tgaaataat cgtgccaca aggcggact 
tggtttcct gaaggcgca caatttgct tggtgtttg 
tgcttgtgg aacaacttt tagacacat tccctctac 
ttggaaatt tcaaaaata gattttaag acaattaat 
tatctaacc agtgtggat aattgttcc tcttgatgt 
attcgtttt ggaatacgt ggtttaggt tttattcat 
ttggaaaag tgcaatgtc tgtttaaat ttttatgtt 
tacatggag ttgctcatt gatgtatta taaaagttt 
ttaaacaaa tacaacgtc ttttattct
    • >TC008204 RB
      aaccaagtg ttcatagct tcctcagtc gaattatat 
atctccggg gaaaacaaa gctccggag ccccgactg 
ttcaagtgg tgctccgga tgttcgaat cccagagtg 
aaaaatcga agcacttga cgaaaatac tgaacggcc 
tccaggttt gaaataaac caaatttcg cctcaagtg 
ttatgaatt tatatttag caaagtact tgagctgta 
ggaaaacaa caacaaaaa tgaatttaa taggcacgt 
ccctttata taacaggat gctgcacaa tgggatcat 
tttaaattc gtattgtaa ccctatatg tcgtaaata 
aacaaatac agttcaaat tgttgcttc tgtcggtga 
taacacgac acagtgctt gagtgataa gataaatac 
tttaaaaca ttttcgtct ttctgtcat ttcgtgcat 
tcatttcaa caaattgtg acggttttg caacaaata 
tggccggcg agcaaataa ttgcatttg tctgttttc 
cttcaatcg gtcttaatc gcctcgcat ctcattata 
ataaatgag aaaatctcc acgtgaaaa tacaaaaaa 
taccgagcg tctcgagcg tgcgtactc gcagctgat 
tttcaactt gtagggttc ggggatata atataaaag 
tgcttgcga tgtaaaagc agaagtcgc tgaaacttt 
gccagtttc aatccgggt attttcgtg cggttgtaa 
ggattcagt gcttgtgtg tttgcaaca attaatcga 
aatggcggt tcaaatgca gcctagttt catcctcac 
cgagaagtt cgaaaagct caaattcac cgattttgt 
gtccgtcga ttcagtctc caccccctc gacaccttc 
caccccctg cagcccccc agccgagcc cttcagcat 
cgaagtgat cacagcggg ggacgcgga cgaagtctt 
gcggttctt gcggtgttt tttcatccg agatgagcc 
cctgaacca cagtattgg gcttctgga cggcaagga 
aacctgtcc ggagctgga gtcgtattc catcaagga 
tttagacag tggacttaa tctcaaggc ggtttccaa 
cgggaagat aatcggggt ttgcctcaa cggcatcct 
gactcgggg ctatctgga ggagatcga agaagcgaa 
ctgtgagga caaaaagtt cgagtcgat tttgaggct 
cctcgacca tgtcgcagt ccagtcgga cattttctc 
gcatttccc ggacgtgga taaagccat ggttgtgaa 
aatcctctc tgtggatag ctctctacg aggacgagg 
gatcgcaaa ggatctgat gaataggac gagagacct 
tgcccgaga actgggctg cggtataat gaccgccga 
ttgcacgag tcacttcac agcaagggc tctgaagaa 
gctgggatt tgaatgcat ttactctct caattacga 
agactacaa agttaatgg agaagtcgt gtttacgcc 
ggagacacc ccatgccgc cgtcactgt ttacacgca 
aagaattgt gaaataatc gtgccacaa ggcggactt 
ggtttcctg aaggcgcac aatttgctt ggtgtttgt 
gcttgtgga acaactttt agacacatt ccctctact 
tggaaattt caaaaatag attttaaga caattaatt 
atctaacca gtgtggata attgttcct cttgatgta 
ttcgttttg gaatacgtg gtttaggtt ttattcatt 
tggaaaagt gcaatgtct gtttaaatt tttatgttt 
acatggagt tgctcattg atgtattat aaaagtttt 
taaacaaat acaacgtct tttattct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY