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Gene information
General information
TC008189 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6348509..6351893
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008189
    iBeetle screen iB_04442

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008189 RA
      MSIQYGRVV SLILLERFG PVVEKVGTY LFQYGTVPL 
      LYIKKHTDL PISKVKEAL CILIKYRLV TFTPNRNEN 
      VANYTLQHE RVLLMLRYA KYINLIKKK FGTECELIM 
      EELLQRSYW TASELILKV IERLKKDHN KNVTILALR 
      EKIFSLLAA QYLIRLPYS MEDKPVPSL VIKETEQFS 
      PPTIDFKQI ALVQSNKAP VDSLPDQGV YWTVNFDRF 
      HQDMRDKLI VNAFIKKFD ENAGEFVRL LLQQMYIRT 
      QPWAEVSNP VPIVEIRDL VRKQATHQH LLAFFDQYV 
      NVLEQDSSK LIRQSGEAG GGSFQIFLK ETFTQFAWE 
      TVEQIVLEK FDTKAARIF RLVKSKQYI EPDQIQQLA 
      MIPAKEAKR LSYQLLEEN FLQVQDLKK STSNGPNKS 
      FTLFYTNLD QIVRMTLEL CYKTLFNIM TRKNHERLV 
      HKRIIDKKQ RVDTIIMGM RAQGAADEQ LSDIEEMIT 
      PPEREILQK IDVIMKKLN TVELEVDET IFLLQMYLI 
      YQ
    CDS sequence
    • >TC008189 RA
      atgtcgatt cagtacgga agggttgtt tcccttatt 
      ttactcgag cgtttcggc cccgtggtc gaaaaggtg 
      ggcacttac ttgtttcag tacggaact gtgcccctt 
      ctgtacatc aaaaagcac acagatttg cccatatct 
      aaggtcaag gaagcttta tgtatttta ataaagtac 
      agattggtc acgttcacc cccaatcgc aatgaaaac 
      gttgcaaat tatacactg caacatgag cgtgtttta 
      ttaatgttg cgctacgcc aaatacatt aatttaatt 
      aaaaagaaa tttgggact gagtgtgaa ttaattatg 
      gaagagttg ttacagcgg agctactgg acggcgtct 
      gaattaatc ctgaaagtg attgagaga ctgaaaaaa 
      gatcataac aaaaacgtg acaatttta gctttaaga 
      gaaaaaatt ttttcattg ttggccgcg cagtatttg 
      atacggttg ccgtatagt atggaagat aaacctgtt 
      cctagttta gtgataaaa gagacggaa cagttttca 
      cctccgaca attgatttt aaacaaatt gccttagtt 
      cagagcaat aaagcacca gtggattct ctccctgac 
      cagggcgtt tactggact gttaacttc gataggttt 
      catcaagac atgagagat aaattaatt gtgaacgct 
      tttattaaa aagtttgac gaaaacgcg ggtgaattt 
      gtcagactt ttgctacaa caaatgtac attaggaca 
      caaccatgg gctgaggtc tcgaatcca gtcccaatt 
      gtcgaaatt cgggacctt gtccggaaa caagccact 
      catcaacac cttttggcg ttttttgac caatacgtt 
      aatgtcttg gagcaagac agttctaaa ttaattaga 
      cagtcggga gaggcaggt ggcggttct tttcaaatt 
      tttctgaaa gagacgttc actcaattt gcatgggag 
      actgtggaa cagatagtt ttggaaaaa ttcgatact 
      aaagccgcc cggattttc cgtcttgtt aagtcgaaa 
      caatacata gaaccggat cagattcaa caactggca 
      atgattccg gctaaagaa gcaaaacgg ctctcttat 
      caactcctg gaagagaat tttttgcaa gtccaggat 
      ttgaaaaaa tcgacaagt aatggccct aataagagt 
      tttactttg ttttataca aacttggac caaatcgtg 
      cgaatgact ctagaattg tgttataaa actttattt 
      aatattatg actagaaaa aatcacgaa aggttggtt 
      cataagaga attatcgat aagaaacaa cgtgttgac 
      acgattatt atggggatg agggcacag ggggcggca 
      gacgagcaa ttatctgac attgaggaa atgatcacg 
      ccaccagaa cgagaaatt ttgcagaaa atcgacgtg 
      attatgaag aaattaaac accgtcgag ttggaagtc 
      gacgagacg atatttttg ttacaaatg tatttaatt 
      tatcaataa
    mRNA sequence
    • >TC008189 RA
      ctgtatttc tacattttg acaagcagc ctaacctaa 
      ttttttcta agtaatcac attgcaata tttttgatt 
      ttatcccaa tgtcgattc agtacggaa gggttgttt 
      cccttattt tactcgagc gtttcggcc ccgtggtcg 
      aaaaggtgg gcacttact tgtttcagt acggaactg 
      tgccccttc tgtacatca aaaagcaca cagatttgc 
      ccatatcta aggtcaagg aagctttat gtattttaa 
      taaagtaca gattggtca cgttcaccc ccaatcgca 
      atgaaaacg ttgcaaatt atacactgc aacatgagc 
      gtgttttat taatgttgc gctacgcca aatacatta 
      atttaatta aaaagaaat ttgggactg agtgtgaat 
      taattatgg aagagttgt tacagcgga gctactgga 
      cggcgtctg aattaatcc tgaaagtga ttgagagac 
      tgaaaaaag atcataaca aaaacgtga caattttag 
      ctttaagag aaaaaattt tttcattgt tggccgcgc 
      agtatttga tacggttgc cgtatagta tggaagata 
      aacctgttc ctagtttag tgataaaag agacggaac 
      agttttcac ctccgacaa ttgatttta aacaaattg 
      ccttagttc agagcaata aagcaccag tggattctc 
      tccctgacc agggcgttt actggactg ttaacttcg 
      ataggtttc atcaagaca tgagagata aattaattg 
      tgaacgctt ttattaaaa agtttgacg aaaacgcgg 
      gtgaatttg tcagacttt tgctacaac aaatgtaca 
      ttaggacac aaccatggg ctgaggtct cgaatccag 
      tcccaattg tcgaaattc gggaccttg tccggaaac 
      aagccactc atcaacacc ttttggcgt tttttgacc 
      aatacgtta atgtcttgg agcaagaca gttctaaat 
      taattagac agtcgggag aggcaggtg gcggttctt 
      ttcaaattt ttctgaaag agacgttca ctcaatttg 
      catgggaga ctgtggaac agatagttt tggaaaaat 
      tcgatacta aagccgccc ggattttcc gtcttgtta 
      agtcgaaac aatacatag aaccggatc agattcaac 
      aactggcaa tgattccgg ctaaagaag caaaacggc 
      tctcttatc aactcctgg aagagaatt ttttgcaag 
      tccaggatt tgaaaaaat cgacaagta atggcccta 
      ataagagtt ttactttgt tttatacaa acttggacc 
      aaatcgtgc gaatgactc tagaattgt gttataaaa 
      ctttattta atattatga ctagaaaaa atcacgaaa 
      ggttggttc ataagagaa ttatcgata agaaacaac 
      gtgttgaca cgattatta tggggatga gggcacagg 
      gggcggcag acgagcaat tatctgaca ttgaggaaa 
      tgatcacgc caccagaac gagaaattt tgcagaaaa 
      tcgacgtga ttatgaaga aattaaaca ccgtcgagt 
      tggaagtcg acgagacga tatttttgt tacaaatgt 
      atttaattt atcaataaa ttcgatttt tgaaaatgc 
      ctagtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY