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Gene information
General information
TC008187 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6290440..6292497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008187
      iBeetle screen iB_04441

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008187 RA
        MNMRMFMAC VVLIAVYAN TVTAEADLQ EQNENQYIN 
        PVNDYEHEH PTKEQDKRT IFPSHHGWA KHIGIHPSG 
        FKYVIGYGP VDLKYVPVV KPVSIKAHP HHHYHHHKV 
        HIKRPVVLR PLLPVRPVI HQTGWKPVV PLVKPVHVK 
        PVHHVKPVH VHHDHVKPV HVHPVHVHH DHVKPVHVH 
        HDHVKPVHV HHDHVKPVH VHHDHVKPV HVHVHHDHV 
        KPVHVHPVH VPVKPVVPI VPSKPLIPV AQPVPLVPV 
        APAIKPLHP VPVVPPPVF QIIPTGPGG VPLHNPALI 
        GQYAPFVHH QHQVFVPQS VVPVHAHPQ PPHIHPGVP 
        IHAHPTHIH PQIPHVQHD VQTPLLPHD HPIFHQHPH 
        AHIPDHPLV HQHPHAHIP EFYHQQHVP VQHLHPEQH 
        PEVFHQPEV PVPDPAIFQ QQLPHDPNS VYHRHPDPP 
        LPQHAPTLF NQQINGDVQ HHFSQNIQH GDSATSSII 
        LQGAIQPGH TDVQIPLHL PHHPNYAPD EGRHFHPGN 
        FHDPSQHPE IVAPNISLE PPFTHKK
      CDS sequence
      • >TC008187 RA
        atgaacatg aggatgttt atggcatgt gttgtgcta 
        attgccgtt tatgccaac actgtaacg gccgaagct 
        gatttacaa gaacaaaac gaaaaccaa tacataaat 
        cctgtcaac gattacgaa catgaacat ccgaccaaa 
        gaacaagat aaaagaact atttttccc agccaccat 
        ggatgggcc aaacacatc ggtattcac ccatctggg 
        ttcaaatac gtcatcggg tacggaccc gttgacctg 
        aaatacgta ccggtggta aaacctgtc agtattaag 
        gcccatcct caccaccac taccaccat cacaaagtt 
        cacataaaa cggcccgtg gtgcttagg ccactgctg 
        cccgttcgg ccggtgatt caccaaacc ggctggaag 
        ccggtggtt cccctggtt aaaccagtg catgttaaa 
        cctgtccat catgttaaa cctgttcat gttcaccac 
        gaccacgtt aaacctgtt catgttcat cctgtgcat 
        gttcaccac gaccacgtt aaacctgtt catgtccac 
        cacgaccac gttaaaccc gttcatgtt caccacgac 
        cacgttaaa cccgttcat gttcaccac gaccacgtc 
        aaacccgtt catgttcat gttcaccac gatcatgtt 
        aaacccgtt catgttcat ccagttcat gttcccgtt 
        aaaccagta gttccgatt gtaccatca aaaccttta 
        attcctgtg gcacaaccc gtaccatta gtcccagtt 
        gccccagct atcaaacct ttgcaccca gttccagtg 
        gtaccaccc cctgtattc cagataatc ccaactggt 
        cctggaggg gtaccatta cataatccc gctttaatt 
        ggacaatat gctcctttt gtgcaccat caacatcaa 
        gtttttgtt ccccagtca gtggtacca gtacatgca 
        cacccgcag ccaccccac atacaccca ggagtacca 
        atccacgca caccctaca catattcac ccccaaata 
        ccccacgta caacacgac gttcaaact cctttactc 
        ccccatgac catcccatt ttccaccag cacccccat 
        gcccacatt cccgaccat ccccttgtg catcaacac 
        ccccacgcc catattccc gaattttac caccaacaa 
        cacgttccc gttcaacat ttacaccca gaacaacat 
        cccgaagtg ttccaccaa ccggaagtc ccagttcca 
        gatcccgca atttttcag caacagtta ccccatgac 
        cctaattct gtttatcac cgccaccct gatccacca 
        ctaccccaa catgcccca actctcttc aaccaacaa 
        ataaacgga gatgtgcaa caccacttt agtcaaaat 
        attcaacat ggggattcc gctacttct agtattatt 
        ttacaagga gccattcaa ccgggacat acagacgtc 
        cagattcct ttacattta ccccatcat cccaattat 
        gcgccagat gaagggaga catttccat ccaggaaat 
        ttccatgat ccttcacaa catcccgaa attgtggct 
        ccgaatatt tcactggaa ccgccattt acacacaag 
        aaatag
      mRNA sequence
      • >TC008187 RA
        tgtttgtat aaaaacaaa aagaaagtg cccgtttcg 
        tttttgtta ttgtaaacc tgcagtgtg atggtgttg 
        gtttagttc tttgatttt tgaatacag tgtgttaca 
        agtgcaaga atgaacatg aggatgttt atggcatgt 
        gttgtgcta attgccgtt tatgccaac actgtaacg 
        gccgaagct gatttacaa gaacaaaac gaaaaccaa 
        tacataaat cctgtcaac gattacgaa catgaacat 
        ccgaccaaa gaacaagat aaaagaact atttttccc 
        agccaccat ggatgggcc aaacacatc ggtattcac 
        ccatctggg ttcaaatac gtcatcggg tacggaccc 
        gttgacctg aaatacgta ccggtggta aaacctgtc 
        agtattaag gcccatcct caccaccac taccaccat 
        cacaaagtt cacataaaa cggcccgtg gtgcttagg 
        ccactgctg cccgttcgg ccggtgatt caccaaacc 
        ggctggaag ccggtggtt cccctggtt aaaccagtg 
        catgttaaa cctgtccat catgttaaa cctgttcat 
        gttcaccac gaccacgtt aaacctgtt catgttcat 
        cctgtgcat gttcaccac gaccacgtt aaacctgtt 
        catgtccac cacgaccac gttaaaccc gttcatgtt 
        caccacgac cacgttaaa cccgttcat gttcaccac 
        gaccacgtc aaacccgtt catgttcat gttcaccac 
        gatcatgtt aaacccgtt catgttcat ccagttcat 
        gttcccgtt aaaccagta gttccgatt gtaccatca 
        aaaccttta attcctgtg gcacaaccc gtaccatta 
        gtcccagtt gccccagct atcaaacct ttgcaccca 
        gttccagtg gtaccaccc cctgtattc cagataatc 
        ccaactggt cctggaggg gtaccatta cataatccc 
        gctttaatt ggacaatat gctcctttt gtgcaccat 
        caacatcaa gtttttgtt ccccagtca gtggtacca 
        gtacatgca cacccgcag ccaccccac atacaccca 
        ggagtacca atccacgca caccctaca catattcac 
        ccccaaata ccccacgta caacacgac gttcaaact 
        cctttactc ccccatgac catcccatt ttccaccag 
        cacccccat gcccacatt cccgaccat ccccttgtg 
        catcaacac ccccacgcc catattccc gaattttac 
        caccaacaa cacgttccc gttcaacat ttacaccca 
        gaacaacat cccgaagtg ttccaccaa ccggaagtc 
        ccagttcca gatcccgca atttttcag caacagtta 
        ccccatgac cctaattct gtttatcac cgccaccct 
        gatccacca ctaccccaa catgcccca actctcttc 
        aaccaacaa ataaacgga gatgtgcaa caccacttt 
        agtcaaaat attcaacat ggggattcc gctacttct 
        agtattatt ttacaagga gccattcaa ccgggacat 
        acagacgtc cagattcct ttacattta ccccatcat 
        cccaattat gcgccagat gaagggaga catttccat 
        ccaggaaat ttccatgat ccttcacaa catcccgaa 
        attgtggct ccgaatatt tcactggaa ccgccattt 
        acacacaag aaataggat tgtttctgt tatgtttta 
        gtatttatt tttataaag tgtaaataa ttaaagtga 
        cacgaagtt atcgcgcac cgggtgctt gtttaagcg 
        agtctgaaa atacgggga tattttacc aaatgtatt 
        ctttttata ctcttgttt ttccaatgt acaaatgta 
        ggatgtatt tttgtaaat aatcgcttc aggaatgca 
        gtcaataaa tctgtgatt aatgt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY