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Gene information
General information
TC008185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6245670..6259487
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008185
    iBeetle screen iB_04440

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    urogomphi size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi orientation irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: some larva developed to L2 larva and than died. looked normal and couldn't go through sieve (maybe CNS defect)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 9


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008185 RA
      MFAGGRELK PSRMEVLSI LLKNPQHHS LPHSMPVHL 
      LNGTYHITS FDGSTTIKE FQDSLAHEI CCRVSNGFA 
      IFSDDPIEK DLEHTLDPN AKLCDLISK WEIALREKG 
      LGKFENSRV IRLTYKNRL WWKNSARLE TDKERLLLC 
      YQVNKQIVS GRFPLSREL ALELAALMA QLDMGDCTF 
      GSHKNTSVN NTLSNQGTS NRHQSPKST TNGTISPPT 
      AIQNSNTLS ALNNVHGIL TAHSNQALN AIDKFYPYR 
      YRDQLTQEG LTELQAKLL EKWRGLKGR TQLDCVRIY 
      LTCTRKWPF FGATLFQAR LRQHDSPMI WLAVNEDSI 
      TILDLATMQ QRVRYPYTN VLTFGGCQE DFMLVVTQN 
      DQQPSQKLI FSLSKPKIL ELTLLIADY MNLLINNPG 
      TPLLGTLSR GTMVSVNQS VVNQSIISQ AIANQSQPD 
      ILKSTPDHG VQRSDSKRR THVDSGLA
    • >TC008185 RB
      MLQTFKDSI QLGARANSY RLGNIQYKG SNLHPLFRD 
      GGDIYERLV HCVRSVPEG GTCGLSELE QRVLEAESR 
      AEEAEDKVR IMEQKLAWS QQSDPNNLQ IHRLEGEVE 
      EQRQLRLHD ARQVEAKAA RIKEWVTNK LRELEQQNQ 
      TLREQNIKC NQQLELLRN HLALADRRR SESCSPEPR 
      QHTSTSNST RSNRHRRHQ SVCLSSNFE PAVPSTLVT 
      SVNFGTSNS SMDPLTDEL RAAVDNLSI GRVPSSSAS 
      DPDLAHDYA EIYTPSREK VPWPRAPTP PIHRFPSWE 
      DRIYQVAAD GLTLTGTTP SDIGPEQAG YQDIPVPVY 
      ATVKGRASQ IRSMPFTGD SSDDSSDGE GNNTTAVDG 
      TNTHSRSSG DTSGSSPGK RTASSSSGS PSKSKTRDT 
      SFESGMSDD YAVPPDALS CDTTSLESS MLLRASYLD 
      SPKRIESLE KSGSLAKLG GKLKTWRKK WFVLKNGVL 
      TYYKSQSDI NRKPQGQII LDEVCKITR AEGSNTFEI 
      DTGKKTYYL TADSITAME DWVRILQNV QRRNATKLL 
      LSKEENKPS VQGWLTKVK NGHAKKCWC VLIGKMFLY 
      FKSPTDTTP AGQINMRDA RVEEVEHLS DSDSEERDT 
      EHPDLTIGI FPTNQGPTY LLCLGKQEK DSWLYHLTV 
      ASGGGPSTG TQYEQLIHK LMEVDGDSN CVLWRHPVL 
      LHVPCTKDG AIPSYFPLT TLPTETLQA EAIKLFKCL 
      QLFMSAAVD QAGIDYHVV LAQNALQQC LDMPELQAE 
      LICALIKQT SKSSPLPKL GVQAVQEEK YKEHKECYK 
      EHKDHKGPP SYTLLQGWQ LLALAVSLF VPKSSRLLW 
      FLKLHLQRN ADNRTECGK YAAYCERAL ERTIQTGGR 
      ELKPSRMEV LSILLKNPQ HHSLPHSMP VHLLNGTYH 
      ITSFDGSTT IKEFQDSLA HEICCRVSN GFAIFSDDP 
      IEKDLEHTL DPNAKLCDL ISKWEIALR EKGLGKFEN 
      SRVIRLTYK NRLWWKNSA RLETDKERL LLCYQVNKQ 
      IVSGRFPLS RELALELAA LMAQLDMGD CTFGSHKNT 
      SVNNTLSNQ GTSNRHQSP KSTTNGTIS PPTAIQNSN 
      TLSALNNVH GILTAHSNQ ALNAIDKFY PYRYRDQLT 
      QEGLTELQA KLLEKWRGL KGRTQLDCV RIYLTCTRK 
      WPFFGATLF QARLRQHDS PMIWLAVNE DSITILDLA 
      TMQQRVRYP YTNVLTFGG CQEDFMLVV TQNDQQPSQ 
      KLIFSLSKP KILELTLLI ADYMNLLIN NPGTPLLGT 
      LSRGTMVSV NQSVVNQSI ISQAIANQS QPDILKSTP 
      DHGVQRSDS KRRTHVDSG LA
    • >TC008185 RC
      MLQTFKDSI QLGARANSY RLGNIQYKG SNLHPLFRD 
      GGDIYERLV HCVRSVPEG GTCGLSELE QRVLEAESR 
      AEEAEDKVR IMEQKLAWS QQSDPNNLQ IHRLEGEVE 
      EQRQLRLHD ARQVEAKAA RIKEWVTNK LRELEQQNQ 
      TLREQNIKC NQQLELLRN HLALADRRR SESCSPEPR 
      QHTSTSNST RSNRHRRHQ SVCLSSNFE PAVPSTLVT 
      SVNFGTSNS SMDPLTDEL RAAVDNLSI GRVPSSSAS 
      DPDLAHDYA EIYTPSREK VPWPRAPTP PIHRFPSWE 
      DRIYQVAAD GLTLTGTTP SDIGPEQAG YQDIPVPVY 
      ATVKGRASQ IRSMPFTGD SSDDSSDGE GNNTTAVDG 
      TNTHSRSSG DTSGSSPGK RTASSSSGS PSKSKTRDT 
      SFESGMSDD YAVPPDALS CDTTSLESS MLLRASYLD 
      SPKRIESLE KSGSLAKLG GKLKTWRKK WFVLKNGVL 
      TYYKSQSDI NRKPQGQII LDEVCKITR AEGSNTFEI 
      DTGKKTYYL TADSITAME DWVRILQNV QRRNATKLL 
      LSKEENKPS VQGWLTKVK NGHAKKCWC VLIGKMFLY 
      FKSPTDTTP AGQINMRDA RVEEVEHLS DSDSEERDT 
      EHPDLTIGI FPTNQGPTY LLCLGKQEK DSWLYHLTV 
      ASGGGPSTG TQYEQLIHK LMEVDGDSN CVLWRHPVL 
      LHVPCTKDG AIPSYFPLT TLPTETLQA EAIKLFKCL 
      QLFMSAAVD QAGIDYHVV LAQNALQQC LDMPELQAE 
      LICALIKQT SKSSPLPKL GVQAVQEEK YKEHKECYK 
      EHKDHKGPP SYTLLQGWQ LLALAVSLF VPKSSRLLW 
      FLKLHLQRN ADNRTECGK YAAYCERAL ERTIQTG
    CDS sequence
    • >TC008185 RA
      atgtttgca ggaggtcgc gagttgaag ccgtctcgc 
      atggaagtc ctcagcatc ctgctgaag aacccgcaa 
      caccactct ttgccccat tcgatgccc gtccacctc 
      ctcaacggc acatatcac ataacgagt ttcgacggc 
      tctaccaca atcaaagag tttcaggat agtctagcg 
      cacgagatt tgctgtcgg gtcagcaac ggcttcgcg 
      atttttagc gatgacccg attgagaaa gacctagag 
      catacgctt gacccgaac gcgaaactt tgtgatttg 
      atatcgaaa tgggagatt gccttacgt gagaaaggc 
      ttagggaaa tttgaaaac agtcgtgtg attcgactg 
      acttacaaa aatcgactg tggtggaag aacagtgct 
      cgcttagaa acggacaaa gagaggtta cttctgtgc 
      taccaagtc aataaacag attgtttcg ggacggttt 
      ccgctaagc cgggaactg gccttggag ttggcagca 
      ctgatggca cagctcgat atgggcgat tgcaccttc 
      gggtcgcat aaaaacact agtgtaaat aatacttta 
      agtaatcaa gggacttcc aatcgacac caaagcccc 
      aaatcgacg acgaatggg acgataagc ccgccaact 
      gccattcaa aattctaat actctaagc gccttgaat 
      aacgtgcac gggatcctt acagctcac tcgaatcaa 
      gctctcaac gccattgat aagttctat ccgtacaga 
      tatcgggat cagttgacg caagaaggc ctaacggag 
      ctgcaagca aagcttttg gagaagtgg cgagggctc 
      aaaggccga actcagcta gattgtgtc cggatttac 
      ctcacctgc acccgcaaa tggcccttc ttcggggcc 
      acgctcttc caggcgcgt ttgcgccag cacgactcg 
      cccatgatc tggctggcg gtgaacgaa gactccatc 
      acaatcctc gaccttgca acgatgcag caacgcgtc 
      agataccca tacaccaac gtgctcaca ttcggcggt 
      tgtcaagaa gacttcatg ttggtcgtg acccaaaac 
      gaccaacaa ccctcccaa aaactaatc ttttcgtta 
      agtaaaccg aaaatactc gaactgact ttactcata 
      gctgactac atgaatctc ttgataaat aatcccggt 
      acgcctttg ttaggtact ttaagtagg ggaacaatg 
      gtttccgtt aatcagtct gtggtaaat caatctatc 
      attagccaa gctatcgcc aatcaaagt caacccgat 
      attttaaaa tcgacgcct gatcatggg gtgcagagg 
      tcggattcg aagaggagg acgcatgtg gactcggga 
      cttgcgtga
    • >TC008185 RB
      atgcttcaa acattcaaa gactcaatt caattaggg 
      gcgagagca aatagctac cggctaggc aacatacaa 
      tacaaggga tcgaatttg catccccta tttcgcgac 
      gggggcgac atctacgag agactagtc cactgcgtc 
      cgttcggtg ccagagggc ggcacctgt ggtctttcc 
      gaactggaa cagagggtt ttggaggcg gaaagtcga 
      gcggaagaa gccgaggac aaggtgcga ataatggaa 
      cagaagctt gcatggtcg cagcaaagt gacccgaac 
      aacctccag atccaccgt ttagagggc gaagtggaa 
      gagcaacgc cagttgcga ctccacgac gcccgccaa 
      gtcgaagcc aaagcggcc cgcatcaaa gaatgggtg 
      acgaataaa ctccgcgaa ctcgaacaa caaaaccaa 
      acccttcgc gaacagaat atcaagtgc aatcaacaa 
      ctggaactg ctccgaaac cacttagcc ctggcggac 
      cgccggaga tcagagagc tgctcacca gagcccaga 
      caacacaca tccacttcc aattcgacg aggagcaat 
      cgacacagg agacatcag tcggtttgc ctgagcagt 
      aatttcgag ccggcagtg ccgagcact cttgtaaca 
      agcgtcaat ttcggaacg agtaattcg agcatggat 
      ccactgacg gacgagctg agagcggcg gttgataat 
      ctgagcatt gggagggtt cccagcagc agtgcttct 
      gatccggat ttggcacat gattatgcc gagatttac 
      acgcccagc agagaaaaa gtcccctgg cctagggct 
      cccacgccg ccgattcac aggttcccc agctgggag 
      gatcggatt tatcaggta gcggctgac gggttaacg 
      ttgaccgga accaccccc agcgacatc ggcccggag 
      caggcgggg taccaggac atcccagtt ccggtctac 
      gctactgtc aaaggccgg gcgagccag atccggtcc 
      atgcccttc actggcgac tcttcggac gattcgagt 
      gacggtgaa ggtaataac accaccgct gtcgacggt 
      accaatact catagtaga agttccggt gatacttcg 
      ggttcaagt ccggggaaa cggacggct tccagtagc 
      agtggatcc ccgagtaaa agcaaaact cgagacact 
      tcgttcgag agtggcatg tcggatgat tatgccgta 
      ccaccggat gctctcagt tgtgatacg actagtcta 
      gaatcgtca atgttgctt agagcgtct tatttagat 
      agtcccaag cggattgaa agtttagaa aaaagtggg 
      tctttagca aaattaggg gggaagtta aaaacgtgg 
      cgtaagaaa tggttcgtg ttgaaaaac ggcgtcttg 
      acttactac aagagtcaa agtgatata aaccgcaaa 
      cctcagggt caaataatc ttagacgaa gtttgtaaa 
      ataacgaga gccgaaggc tccaacact ttcgagata 
      gacacgggc aaaaaaacg tactattta acagccgac 
      tcaataact gcgatggaa gactgggtt agaatcctt 
      caaaacgta caacgaaga aacgcaacc aagttatta 
      ctgagcaag gaagagaac aaaccgtca gttcaagga 
      tggctaacg aaagtgaaa aatggtcac gcgaaaaaa 
      tgctggtgc gtgctaatc ggaaaaatg tttttgtat 
      tttaaatcg ccaacagac acgacgcca gcaggtcaa 
      atcaatatg cgtgacgcg cgggttgaa gaagtcgag 
      catttatca gattccgat tccgaggaa cgagatacg 
      gaacatccg gacttaacc atcgggata tttcccaca 
      aatcaaggc cctacgtat ttattgtgt ttagggaaa 
      caagagaaa gattcgtgg ctgtatcat ttgactgta 
      gccagcggc ggcggcccc agcactggc acacaatac 
      gaacaattg atacataaa ttgatggag gtcgatggc 
      gattcgaac tgcgtcttg tggaggcat ccggtgttg 
      ttacatgtg ccttgtacg aaagacggg gctatacct 
      tcgtatttc ccgcttaca acactaccg actgaaacg 
      ctacaggcc gaagcaatc aaactattc aaatgtctg 
      caattattc atgtcggcg gctgttgac caagccgga 
      atcgattat cacgtagtg ttggcacaa aatgcattg 
      cagcaatgt ttggacatg ccggagcta caggccgaa 
      ctcatttgt gcgttaatt aagcaaacg agcaaaagc 
      tcgcccttg cccaagctc ggagtccag gcggtccag 
      gaagagaaa tacaaagag cacaaggaa tgttacaag 
      gagcataaa gaccataaa ggacccccg agttacacg 
      ttgttgcaa ggatggcag ttgctcgcg ttggctgtc 
      agtttgttc gttccgaag tcgtcaaga ttattgtgg 
      tttttgaag ctgcatctg cagagaaac gctgacaat 
      cggacagag tgtgggaaa tatgcggcg tattgtgag 
      cgggccttg gaaaggacg atacaaaca ggaggtcgc 
      gagttgaag ccgtctcgc atggaagtc ctcagcatc 
      ctgctgaag aacccgcaa caccactct ttgccccat 
      tcgatgccc gtccacctc ctcaacggc acatatcac 
      ataacgagt ttcgacggc tctaccaca atcaaagag 
      tttcaggat agtctagcg cacgagatt tgctgtcgg 
      gtcagcaac ggcttcgcg atttttagc gatgacccg 
      attgagaaa gacctagag catacgctt gacccgaac 
      gcgaaactt tgtgatttg atatcgaaa tgggagatt 
      gccttacgt gagaaaggc ttagggaaa tttgaaaac 
      agtcgtgtg attcgactg acttacaaa aatcgactg 
      tggtggaag aacagtgct cgcttagaa acggacaaa 
      gagaggtta cttctgtgc taccaagtc aataaacag 
      attgtttcg ggacggttt ccgctaagc cgggaactg 
      gccttggag ttggcagca ctgatggca cagctcgat 
      atgggcgat tgcaccttc gggtcgcat aaaaacact 
      agtgtaaat aatacttta agtaatcaa gggacttcc 
      aatcgacac caaagcccc aaatcgacg acgaatggg 
      acgataagc ccgccaact gccattcaa aattctaat 
      actctaagc gccttgaat aacgtgcac gggatcctt 
      acagctcac tcgaatcaa gctctcaac gccattgat 
      aagttctat ccgtacaga tatcgggat cagttgacg 
      caagaaggc ctaacggag ctgcaagca aagcttttg 
      gagaagtgg cgagggctc aaaggccga actcagcta 
      gattgtgtc cggatttac ctcacctgc acccgcaaa 
      tggcccttc ttcggggcc acgctcttc caggcgcgt 
      ttgcgccag cacgactcg cccatgatc tggctggcg 
      gtgaacgaa gactccatc acaatcctc gaccttgca 
      acgatgcag caacgcgtc agataccca tacaccaac 
      gtgctcaca ttcggcggt tgtcaagaa gacttcatg 
      ttggtcgtg acccaaaac gaccaacaa ccctcccaa 
      aaactaatc ttttcgtta agtaaaccg aaaatactc 
      gaactgact ttactcata gctgactac atgaatctc 
      ttgataaat aatcccggt acgcctttg ttaggtact 
      ttaagtagg ggaacaatg gtttccgtt aatcagtct 
      gtggtaaat caatctatc attagccaa gctatcgcc 
      aatcaaagt caacccgat attttaaaa tcgacgcct 
      gatcatggg gtgcagagg tcggattcg aagaggagg 
      acgcatgtg gactcggga cttgcgtga
    • >TC008185 RC
      atgcttcaa acattcaaa gactcaatt caattaggg 
      gcgagagca aatagctac cggctaggc aacatacaa 
      tacaaggga tcgaatttg catccccta tttcgcgac 
      gggggcgac atctacgag agactagtc cactgcgtc 
      cgttcggtg ccagagggc ggcacctgt ggtctttcc 
      gaactggaa cagagggtt ttggaggcg gaaagtcga 
      gcggaagaa gccgaggac aaggtgcga ataatggaa 
      cagaagctt gcatggtcg cagcaaagt gacccgaac 
      aacctccag atccaccgt ttagagggc gaagtggaa 
      gagcaacgc cagttgcga ctccacgac gcccgccaa 
      gtcgaagcc aaagcggcc cgcatcaaa gaatgggtg 
      acgaataaa ctccgcgaa ctcgaacaa caaaaccaa 
      acccttcgc gaacagaat atcaagtgc aatcaacaa 
      ctggaactg ctccgaaac cacttagcc ctggcggac 
      cgccggaga tcagagagc tgctcacca gagcccaga 
      caacacaca tccacttcc aattcgacg aggagcaat 
      cgacacagg agacatcag tcggtttgc ctgagcagt 
      aatttcgag ccggcagtg ccgagcact cttgtaaca 
      agcgtcaat ttcggaacg agtaattcg agcatggat 
      ccactgacg gacgagctg agagcggcg gttgataat 
      ctgagcatt gggagggtt cccagcagc agtgcttct 
      gatccggat ttggcacat gattatgcc gagatttac 
      acgcccagc agagaaaaa gtcccctgg cctagggct 
      cccacgccg ccgattcac aggttcccc agctgggag 
      gatcggatt tatcaggta gcggctgac gggttaacg 
      ttgaccgga accaccccc agcgacatc ggcccggag 
      caggcgggg taccaggac atcccagtt ccggtctac 
      gctactgtc aaaggccgg gcgagccag atccggtcc 
      atgcccttc actggcgac tcttcggac gattcgagt 
      gacggtgaa ggtaataac accaccgct gtcgacggt 
      accaatact catagtaga agttccggt gatacttcg 
      ggttcaagt ccggggaaa cggacggct tccagtagc 
      agtggatcc ccgagtaaa agcaaaact cgagacact 
      tcgttcgag agtggcatg tcggatgat tatgccgta 
      ccaccggat gctctcagt tgtgatacg actagtcta 
      gaatcgtca atgttgctt agagcgtct tatttagat 
      agtcccaag cggattgaa agtttagaa aaaagtggg 
      tctttagca aaattaggg gggaagtta aaaacgtgg 
      cgtaagaaa tggttcgtg ttgaaaaac ggcgtcttg 
      acttactac aagagtcaa agtgatata aaccgcaaa 
      cctcagggt caaataatc ttagacgaa gtttgtaaa 
      ataacgaga gccgaaggc tccaacact ttcgagata 
      gacacgggc aaaaaaacg tactattta acagccgac 
      tcaataact gcgatggaa gactgggtt agaatcctt 
      caaaacgta caacgaaga aacgcaacc aagttatta 
      ctgagcaag gaagagaac aaaccgtca gttcaagga 
      tggctaacg aaagtgaaa aatggtcac gcgaaaaaa 
      tgctggtgc gtgctaatc ggaaaaatg tttttgtat 
      tttaaatcg ccaacagac acgacgcca gcaggtcaa 
      atcaatatg cgtgacgcg cgggttgaa gaagtcgag 
      catttatca gattccgat tccgaggaa cgagatacg 
      gaacatccg gacttaacc atcgggata tttcccaca 
      aatcaaggc cctacgtat ttattgtgt ttagggaaa 
      caagagaaa gattcgtgg ctgtatcat ttgactgta 
      gccagcggc ggcggcccc agcactggc acacaatac 
      gaacaattg atacataaa ttgatggag gtcgatggc 
      gattcgaac tgcgtcttg tggaggcat ccggtgttg 
      ttacatgtg ccttgtacg aaagacggg gctatacct 
      tcgtatttc ccgcttaca acactaccg actgaaacg 
      ctacaggcc gaagcaatc aaactattc aaatgtctg 
      caattattc atgtcggcg gctgttgac caagccgga 
      atcgattat cacgtagtg ttggcacaa aatgcattg 
      cagcaatgt ttggacatg ccggagcta caggccgaa 
      ctcatttgt gcgttaatt aagcaaacg agcaaaagc 
      tcgcccttg cccaagctc ggagtccag gcggtccag 
      gaagagaaa tacaaagag cacaaggaa tgttacaag 
      gagcataaa gaccataaa ggacccccg agttacacg 
      ttgttgcaa ggatggcag ttgctcgcg ttggctgtc 
      agtttgttc gttccgaag tcgtcaaga ttattgtgg 
      tttttgaag ctgcatctg cagagaaac gctgacaat 
      cggacagag tgtgggaaa tatgcggcg tattgtgag 
      cgggccttg gaaaggacg atacaaaca ggttag
    mRNA sequence
    • >TC008185 RA
      gaagagaga attggaatg tttgcagga ggtcgcgag 
      ttgaagccg tctcgcatg gaagtcctc agcatcctg 
      ctgaagaac ccgcaacac cactctttg ccccattcg 
      atgcccgtc cacctcctc aacggcaca tatcacata 
      acgagtttc gacggctct accacaatc aaagagttt 
      caggatagt ctagcgcac gagatttgc tgtcgggtc 
      agcaacggc ttcgcgatt tttagcgat gacccgatt 
      gagaaagac ctagagcat acgcttgac ccgaacgcg 
      aaactttgt gatttgata tcgaaatgg gagattgcc 
      ttacgtgag aaaggctta gggaaattt gaaaacagt 
      cgtgtgatt cgactgact tacaaaaat cgactgtgg 
      tggaagaac agtgctcgc ttagaaacg gacaaagag 
      aggttactt ctgtgctac caagtcaat aaacagatt 
      gtttcggga cggtttccg ctaagccgg gaactggcc 
      ttggagttg gcagcactg atggcacag ctcgatatg 
      ggcgattgc accttcggg tcgcataaa aacactagt 
      gtaaataat actttaagt aatcaaggg acttccaat 
      cgacaccaa agccccaaa tcgacgacg aatgggacg 
      ataagcccg ccaactgcc attcaaaat tctaatact 
      ctaagcgcc ttgaataac gtgcacggg atccttaca 
      gctcactcg aatcaagct ctcaacgcc attgataag 
      ttctatccg tacagatat cgggatcag ttgacgcaa 
      gaaggccta acggagctg caagcaaag cttttggag 
      aagtggcga gggctcaaa ggccgaact cagctagat 
      tgtgtccgg atttacctc acctgcacc cgcaaatgg 
      cccttcttc ggggccacg ctcttccag gcgcgtttg 
      cgccagcac gactcgccc atgatctgg ctggcggtg 
      aacgaagac tccatcaca atcctcgac cttgcaacg 
      atgcagcaa cgcgtcaga tacccatac accaacgtg 
      ctcacattc ggcggttgt caagaagac ttcatgttg 
      gtcgtgacc caaaacgac caacaaccc tcccaaaaa 
      ctaatcttt tcgttaagt aaaccgaaa atactcgaa 
      ctgacttta ctcatagct gactacatg aatctcttg 
      ataaataat cccggtacg cctttgtta ggtacttta 
      agtagggga acaatggtt tccgttaat cagtctgtg 
      gtaaatcaa tctatcatt agccaagct atcgccaat 
      caaagtcaa cccgatatt ttaaaatcg acgcctgat 
      catggggtg cagaggtcg gattcgaag aggaggacg 
      catgtggac tcgggactt gcgtgatat tgacgatat 
      cacaaaagt tgcgacggc tgacggtgt cacaagacg 
      gtttcgttt ttttaattg tgatttttc gctttttct 
      gtggcagat cggagatgt atatagcgt gtatgtgga 
      tgtatatag cgactattt attacgttg gacagtttt 
      ataaattgc ttttttggt tgattcgct cgttttttt 
      aatcccggg gatgatcga ggcctacga tttacagta 
      acaactttt atacaaacc taagtgtca ttcaaactg 
      gtgttttga aatagcaaa ttttaaaac atcagcgtg 
      ctaagttga ttttgttgt ggtttccta gtcttttat 
      aaagtgtat gcatgtttg acaatagtt accattatg 
      agatttttt atgatgata caaggggca ccaatattt 
      ttggatgta taagaaaat tttggtgtt ttattttcc 
      tgtaataat cgatctaat aagtgataa ttttctcat 
      atacatttg atattaatg atccctagg tattattaa 
      tttattatt tatatatga ataaagatt ttaaataaa 
      a
    • >TC008185 RB
      gtaaactaa taaagttaa atacgaatg cttcaaaca 
      ttcaaagac tcaattcaa ttaggggcg agagcaaat 
      agctaccgg ctaggcaac atacaatac aagggatcg 
      aatttgcat cccctattt cgcgacggg ggcgacatc 
      tacgagaga ctagtccac tgcgtccgt tcggtgcca 
      gagggcggc acctgtggt ctttccgaa ctggaacag 
      agggttttg gaggcggaa agtcgagcg gaagaagcc 
      gaggacaag gtgcgaata atggaacag aagcttgca 
      tggtcgcag caaagtgac ccgaacaac ctccagatc 
      caccgttta gagggcgaa gtggaagag caacgccag 
      ttgcgactc cacgacgcc cgccaagtc gaagccaaa 
      gcggcccgc atcaaagaa tgggtgacg aataaactc 
      cgcgaactc gaacaacaa aaccaaacc cttcgcgaa 
      cagaatatc aagtgcaat caacaactg gaactgctc 
      cgaaaccac ttagccctg gcggaccgc cggagatca 
      gagagctgc tcaccagag cccagacaa cacacatcc 
      acttccaat tcgacgagg agcaatcga cacaggaga 
      catcagtcg gtttgcctg agcagtaat ttcgagccg 
      gcagtgccg agcactctt gtaacaagc gtcaatttc 
      ggaacgagt aattcgagc atggatcca ctgacggac 
      gagctgaga gcggcggtt gataatctg agcattggg 
      agggttccc agcagcagt gcttctgat ccggatttg 
      gcacatgat tatgccgag atttacacg cccagcaga 
      gaaaaagtc ccctggcct agggctccc acgccgccg 
      attcacagg ttccccagc tgggaggat cggatttat 
      caggtagcg gctgacggg ttaacgttg accggaacc 
      acccccagc gacatcggc ccggagcag gcggggtac 
      caggacatc ccagttccg gtctacgct actgtcaaa 
      ggccgggcg agccagatc cggtccatg cccttcact 
      ggcgactct tcggacgat tcgagtgac ggtgaaggt 
      aataacacc accgctgtc gacggtacc aatactcat 
      agtagaagt tccggtgat acttcgggt tcaagtccg 
      gggaaacgg acggcttcc agtagcagt ggatccccg 
      agtaaaagc aaaactcga gacacttcg ttcgagagt 
      ggcatgtcg gatgattat gccgtacca ccggatgct 
      ctcagttgt gatacgact agtctagaa tcgtcaatg 
      ttgcttaga gcgtcttat ttagatagt cccaagcgg 
      attgaaagt ttagaaaaa agtgggtct ttagcaaaa 
      ttagggggg aagttaaaa acgtggcgt aagaaatgg 
      ttcgtgttg aaaaacggc gtcttgact tactacaag 
      agtcaaagt gatataaac cgcaaacct cagggtcaa 
      ataatctta gacgaagtt tgtaaaata acgagagcc 
      gaaggctcc aacactttc gagatagac acgggcaaa 
      aaaacgtac tatttaaca gccgactca ataactgcg 
      atggaagac tgggttaga atccttcaa aacgtacaa 
      cgaagaaac gcaaccaag ttattactg agcaaggaa 
      gagaacaaa ccgtcagtt caaggatgg ctaacgaaa 
      gtgaaaaat ggtcacgcg aaaaaatgc tggtgcgtg 
      ctaatcgga aaaatgttt ttgtatttt aaatcgcca 
      acagacacg acgccagca ggtcaaatc aatatgcgt 
      gacgcgcgg gttgaagaa gtcgagcat ttatcagat 
      tccgattcc gaggaacga gatacggaa catccggac 
      ttaaccatc gggatattt cccacaaat caaggccct 
      acgtattta ttgtgttta gggaaacaa gagaaagat 
      tcgtggctg tatcatttg actgtagcc agcggcggc 
      ggccccagc actggcaca caatacgaa caattgata 
      cataaattg atggaggtc gatggcgat tcgaactgc 
      gtcttgtgg aggcatccg gtgttgtta catgtgcct 
      tgtacgaaa gacggggct ataccttcg tatttcccg 
      cttacaaca ctaccgact gaaacgcta caggccgaa 
      gcaatcaaa ctattcaaa tgtctgcaa ttattcatg 
      tcggcggct gttgaccaa gccggaatc gattatcac 
      gtagtgttg gcacaaaat gcattgcag caatgtttg 
      gacatgccg gagctacag gccgaactc atttgtgcg 
      ttaattaag caaacgagc aaaagctcg cccttgccc 
      aagctcgga gtccaggcg gtccaggaa gagaaatac 
      aaagagcac aaggaatgt tacaaggag cataaagac 
      cataaagga cccccgagt tacacgttg ttgcaagga 
      tggcagttg ctcgcgttg gctgtcagt ttgttcgtt 
      ccgaagtcg tcaagatta ttgtggttt ttgaagctg 
      catctgcag agaaacgct gacaatcgg acagagtgt 
      gggaaatat gcggcgtat tgtgagcgg gccttggaa 
      aggacgata caaacagga ggtcgcgag ttgaagccg 
      tctcgcatg gaagtcctc agcatcctg ctgaagaac 
      ccgcaacac cactctttg ccccattcg atgcccgtc 
      cacctcctc aacggcaca tatcacata acgagtttc 
      gacggctct accacaatc aaagagttt caggatagt 
      ctagcgcac gagatttgc tgtcgggtc agcaacggc 
      ttcgcgatt tttagcgat gacccgatt gagaaagac 
      ctagagcat acgcttgac ccgaacgcg aaactttgt 
      gatttgata tcgaaatgg gagattgcc ttacgtgag 
      aaaggctta gggaaattt gaaaacagt cgtgtgatt 
      cgactgact tacaaaaat cgactgtgg tggaagaac 
      agtgctcgc ttagaaacg gacaaagag aggttactt 
      ctgtgctac caagtcaat aaacagatt gtttcggga 
      cggtttccg ctaagccgg gaactggcc ttggagttg 
      gcagcactg atggcacag ctcgatatg ggcgattgc 
      accttcggg tcgcataaa aacactagt gtaaataat 
      actttaagt aatcaaggg acttccaat cgacaccaa 
      agccccaaa tcgacgacg aatgggacg ataagcccg 
      ccaactgcc attcaaaat tctaatact ctaagcgcc 
      ttgaataac gtgcacggg atccttaca gctcactcg 
      aatcaagct ctcaacgcc attgataag ttctatccg 
      tacagatat cgggatcag ttgacgcaa gaaggccta 
      acggagctg caagcaaag cttttggag aagtggcga 
      gggctcaaa ggccgaact cagctagat tgtgtccgg 
      atttacctc acctgcacc cgcaaatgg cccttcttc 
      ggggccacg ctcttccag gcgcgtttg cgccagcac 
      gactcgccc atgatctgg ctggcggtg aacgaagac 
      tccatcaca atcctcgac cttgcaacg atgcagcaa 
      cgcgtcaga tacccatac accaacgtg ctcacattc 
      ggcggttgt caagaagac ttcatgttg gtcgtgacc 
      caaaacgac caacaaccc tcccaaaaa ctaatcttt 
      tcgttaagt aaaccgaaa atactcgaa ctgacttta 
      ctcatagct gactacatg aatctcttg ataaataat 
      cccggtacg cctttgtta ggtacttta agtagggga 
      acaatggtt tccgttaat cagtctgtg gtaaatcaa 
      tctatcatt agccaagct atcgccaat caaagtcaa 
      cccgatatt ttaaaatcg acgcctgat catggggtg 
      cagaggtcg gattcgaag aggaggacg catgtggac 
      tcgggactt gcgtgatat tgacgatat cacaaaagt 
      tgcgacggc tgacggtgt cacaagacg gtttcgttt 
      ttttaattg tgatttttc gctttttct gtggcagat 
      cggagatgt atatagcgt gtatgtgga tgtatatag 
      cgactattt attacgttg gacagtttt ataaattgc 
      ttttttggt tgattcgct cgttttttt aatcccggg 
      gatgatcga ggcctacga tttacagta acaactttt 
      atacaaacc taagtgtca ttcaaactg gtgttttga 
      aatagcaaa ttttaaaac atcagcgtg ctaagttga 
      ttttgttgt ggtttccta gtcttttat aaagtgtat 
      gcatgtttg acaatagtt accattatg agatttttt 
      atgatgata caaggggca ccaatattt ttggatgta 
      taagaaaat tttggtgtt ttattttcc tgtaataat 
      cgatctaat aagtgataa ttttctcat atacatttg 
      atattaatg atccctagg tattattaa tttattatt 
      tatatatga ataaagatt ttaaataaa a
    • >TC008185 RC
      gtaaactaa taaagttaa atacgaatg cttcaaaca 
      ttcaaagac tcaattcaa ttaggggcg agagcaaat 
      agctaccgg ctaggcaac atacaatac aagggatcg 
      aatttgcat cccctattt cgcgacggg ggcgacatc 
      tacgagaga ctagtccac tgcgtccgt tcggtgcca 
      gagggcggc acctgtggt ctttccgaa ctggaacag 
      agggttttg gaggcggaa agtcgagcg gaagaagcc 
      gaggacaag gtgcgaata atggaacag aagcttgca 
      tggtcgcag caaagtgac ccgaacaac ctccagatc 
      caccgttta gagggcgaa gtggaagag caacgccag 
      ttgcgactc cacgacgcc cgccaagtc gaagccaaa 
      gcggcccgc atcaaagaa tgggtgacg aataaactc 
      cgcgaactc gaacaacaa aaccaaacc cttcgcgaa 
      cagaatatc aagtgcaat caacaactg gaactgctc 
      cgaaaccac ttagccctg gcggaccgc cggagatca 
      gagagctgc tcaccagag cccagacaa cacacatcc 
      acttccaat tcgacgagg agcaatcga cacaggaga 
      catcagtcg gtttgcctg agcagtaat ttcgagccg 
      gcagtgccg agcactctt gtaacaagc gtcaatttc 
      ggaacgagt aattcgagc atggatcca ctgacggac 
      gagctgaga gcggcggtt gataatctg agcattggg 
      agggttccc agcagcagt gcttctgat ccggatttg 
      gcacatgat tatgccgag atttacacg cccagcaga 
      gaaaaagtc ccctggcct agggctccc acgccgccg 
      attcacagg ttccccagc tgggaggat cggatttat 
      caggtagcg gctgacggg ttaacgttg accggaacc 
      acccccagc gacatcggc ccggagcag gcggggtac 
      caggacatc ccagttccg gtctacgct actgtcaaa 
      ggccgggcg agccagatc cggtccatg cccttcact 
      ggcgactct tcggacgat tcgagtgac ggtgaaggt 
      aataacacc accgctgtc gacggtacc aatactcat 
      agtagaagt tccggtgat acttcgggt tcaagtccg 
      gggaaacgg acggcttcc agtagcagt ggatccccg 
      agtaaaagc aaaactcga gacacttcg ttcgagagt 
      ggcatgtcg gatgattat gccgtacca ccggatgct 
      ctcagttgt gatacgact agtctagaa tcgtcaatg 
      ttgcttaga gcgtcttat ttagatagt cccaagcgg 
      attgaaagt ttagaaaaa agtgggtct ttagcaaaa 
      ttagggggg aagttaaaa acgtggcgt aagaaatgg 
      ttcgtgttg aaaaacggc gtcttgact tactacaag 
      agtcaaagt gatataaac cgcaaacct cagggtcaa 
      ataatctta gacgaagtt tgtaaaata acgagagcc 
      gaaggctcc aacactttc gagatagac acgggcaaa 
      aaaacgtac tatttaaca gccgactca ataactgcg 
      atggaagac tgggttaga atccttcaa aacgtacaa 
      cgaagaaac gcaaccaag ttattactg agcaaggaa 
      gagaacaaa ccgtcagtt caaggatgg ctaacgaaa 
      gtgaaaaat ggtcacgcg aaaaaatgc tggtgcgtg 
      ctaatcgga aaaatgttt ttgtatttt aaatcgcca 
      acagacacg acgccagca ggtcaaatc aatatgcgt 
      gacgcgcgg gttgaagaa gtcgagcat ttatcagat 
      tccgattcc gaggaacga gatacggaa catccggac 
      ttaaccatc gggatattt cccacaaat caaggccct 
      acgtattta ttgtgttta gggaaacaa gagaaagat 
      tcgtggctg tatcatttg actgtagcc agcggcggc 
      ggccccagc actggcaca caatacgaa caattgata 
      cataaattg atggaggtc gatggcgat tcgaactgc 
      gtcttgtgg aggcatccg gtgttgtta catgtgcct 
      tgtacgaaa gacggggct ataccttcg tatttcccg 
      cttacaaca ctaccgact gaaacgcta caggccgaa 
      gcaatcaaa ctattcaaa tgtctgcaa ttattcatg 
      tcggcggct gttgaccaa gccggaatc gattatcac 
      gtagtgttg gcacaaaat gcattgcag caatgtttg 
      gacatgccg gagctacag gccgaactc atttgtgcg 
      ttaattaag caaacgagc aaaagctcg cccttgccc 
      aagctcgga gtccaggcg gtccaggaa gagaaatac 
      aaagagcac aaggaatgt tacaaggag cataaagac 
      cataaagga cccccgagt tacacgttg ttgcaagga 
      tggcagttg ctcgcgttg gctgtcagt ttgttcgtt 
      ccgaagtcg tcaagatta ttgtggttt ttgaagctg 
      catctgcag agaaacgct gacaatcgg acagagtgt 
      gggaaatat gcggcgtat tgtgagcgg gccttggaa 
      aggacgata caaacaggt tagcaattt tttattcac 
      gaaaatttg atataaact agtctattg taagagcag 
      cagcggcat agctcaccg acgagtcgc tcatagaaa 
      cgcattgat tc
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